Article
Oncology
Hong Jiacong, Yang Qirui, Li Haonan, Song Yichang, Chen Yan, Chen Keng
Summary: Osteosarcoma is the most common primary bone cancer in adolescents with a poor prognosis. Current treatments are not satisfactory, therefore, it is critical to develop novel therapeutic strategies. This study discovered that zoledronic acid (ZOL) induces ferroptosis by upregulating POR expression in osteosarcoma cells. Knockdown of POR attenuated ZOL-induced cytotoxicity and the effect of ferroptosis. ZOL also inhibits osteosarcoma growth in vivo. These findings suggest that POR could be used as a therapeutic target to inhibit osteosarcoma.
Article
Biochemistry & Molecular Biology
Merve Demirbugen Oz, Fezile Ozdemir, Kenan Can Tok, Emrah Dural, Yagmur Kir, Muge Ulusoy, Mehmet Gumustas, Bora Baskak, H. Sinan Suzen
Summary: This study found that genetic variations in CYP1A2 and POR did not significantly affect plasma levels of Clozapine (CLZ) and N-desmethylclozapine (DCLZ). However, in the subgroup analysis, the POR*28 genotype significantly influenced CLZ and DCLZ levels in patients who smoked and consumed caffeine. Therefore, individualized treatment with CLZ should take into account both genetic and non-genetic factors.
EXPERT OPINION ON DRUG METABOLISM & TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Bo Yan, Youwei Ai, Qi Sun, Yan Ma, Yang Cao, Jiawen Wang, Zhiyuan Zhang, Xiaodong Wang
Summary: Ferroptosis is a form of necrotic cell death caused by iron-dependent peroxidation of polyunsaturated phospholipids on cell membranes, which is carried out by oxidoreductases transferring electrons to oxygen to generate hydrogen peroxide for lipid peroxidation. This process needs to be counteracted by antioxidant systems to prevent cell death.
Article
Biochemistry & Molecular Biology
Rajalakshmi Kumar, Manikandan Jayaraman, Krishna Ramadas, Adithan Chandrasekaran
Summary: This study used computational methods to identify and analyze deleterious mutations in the human POR gene associated with diseases. The results showed that the A287P and R457H mutations alter the structure and function of POR, leading to hormone production disorders. The findings from these computational analyses are important for predicting potential harmful mutations, understanding the pathological basis of diseases, and explaining the molecular mechanism of drug metabolism.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Multidisciplinary Sciences
Sumiko Ohnami, Akane Naruoka, Mitsuhiro Isaka, Maki Mizuguchi, Sou Nakatani, Fukumi Kamada, Yuji Shimoda, Ai Sakai, Keiichi Ohshima, Keiichi Hatakeyama, Kouji Maruyama, Yasuhisa Ohde, Hirotsugu Kenmotsu, Toshiaki Takahashi, Yasuto Akiyama, Takeshi Nagashima, Kenichi Urakami, Shumpei Ohnami, Ken Yamaguchi
Summary: The differences in genetic susceptibility to lung adenocarcinoma and squamous cell carcinoma are still not clear. Researchers developed a gene sequencing panel to detect germline variants in these two types of cancer. The study found that certain gene variants were associated with an increased risk of squamous cell carcinoma, while whole-gene deletion was associated with adenocarcinoma.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Kathleen Saavedra, Karla Leal, Nicolas Saavedra, Yalena Prado, Isis Paez, Carmen G. Ubilla, Gabriel Rojas, Luis A. Salazar
Summary: The expression of hsa-miR-20a-5p was repressed after atorvastatin treatment in hypercholesteremic patients and in HepG2 cells. In HepG2 cells, repression of hsa-miR-20a-5p increased LDLR gene and protein expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Joana Rita Chora, Mafalda Bourbon
Summary: Recent research has revealed associations between statin pharmacogenomics, but the applicability of this knowledge remains uncertain in high-risk populations. New research methods and insights are helping to better understand the effects of statin therapy and the issue of patients reaching therapeutic target values.
CURRENT OPINION IN LIPIDOLOGY
(2021)
Article
Pharmacology & Pharmacy
Brandon N. S. Ooi, Raechell, Ariel F. Ying, Yong Zher Koh, Yu Jin, Sherman W. L. Yee, Justin H. S. Lee, Samuel S. Chong, Jack W. C. Tan, Jianjun Liu, Caroline G. Lee, Chester L. Drum
Summary: This study identified a combination of 15 SNPs that predict myalgia with good performance in patients on atorvastatin, with potentially functional SNPs outperforming non-functional SNPs. The findings suggest that combinations of potentially functional SNPs could be clinically useful for predicting atorvastatin-induced myalgia.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Emma A. Magavern, David van Heel, Damian Smedley, Mark Caulfield
Summary: This study aimed to explore the possible association between loss-of-function CYP2C19 genotypes and gastrointestinal bleed (GIB) in South Asian participants prescribed antidepressants. The results showed that there was an independent association between antidepressant prescriptions and GIB events, but no relationship between CYP2C19 metabolizer status and GIB in those prescribed antidepressants. Precision dosing based on CYP2C19 genetic information alone is unlikely to reduce GIB prevalence.
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Isis Paez, Yalena Prado, Pia Loren, Carmen G. Ubilla, Nelia Rodriguez, Luis A. Salazar
Summary: This study assessed the expression levels of three long non-coding RNAs (lncRNAs) associated with genes involved in cholesterol homeostasis in hypercholesterolemic patients before and after treatment with atorvastatin. The results showed that these lncRNAs had differential expression in hypercholesterolemic patients and played a role in the variability of response to atorvastatin treatment.
Article
Pharmacology & Pharmacy
Carmen Gloria Ubilla, Yalena Prado, Jeremy Angulo, Ignacio Obreque, Isis Paez, Nicolas Saavedra, Kathleen Saavedra, Tomas Zambrano, Luis A. Salazar
Summary: The study found that in patients with hypercholesterolemia, plasma miRNA-30c-5p and miRNA-29b-3p are overexpressed, and after atorvastatin treatment, three extracellular miRNAs significantly increased, with miRNA-33b-5p showing more than 37-fold increase.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Public, Environmental & Occupational Health
Jessica Bassani Borges, Victor Fernandes de Oliveira, Glaucio Monteiro Ferreira, Bruna Los, Thais Kristini Almendros Afonso Barbosa, Elisangela da Silva Rodrigues Marcal, Carolina Dagli-Hernandez, Renata Caroline Costa de Freitas, Raul Hernandes Bortolin, Augusto Akira Mori, Thiago Dominguez Crespo Hirata, Helder Takashi Imoto Nakaya, Gisele Medeiros Bastos, Helena Strelow Thurow, Rodrigo Marques Goncalves, Daniel Branco de Araujo, Henry Paulo Zatz, Adriana Bertolami, Andre Arpad Faludi, Marcelo Chiara Bertolami, Amanda Guerra de Moraes Rego Sousa, Joao Italo Dias Franca, Cinthia Elim Jannes, Alexandre da Costa Pereira, Marcelo Arruda Nakazone, Doroteia Rossi Silva Souza, Tayanne Silva Carmo, Marcelo Ferraz Sampaio, Renata Gorjao, Tania Cristina Pithon-Curi, Patricia Moriel, Vivian Nogueira Silbiger, Andre Ducati Luchessi, Jessica Nayara Goes de Araujo, Michel Satya Naslavsky, Jaqueline Yu Ting Wang, Thales Kronenberger, Alvaro Cerda, Hui Tzu Lin-Wang, Adriana Regina Garofalo, Cristina Moreno Fajardo, Rosario Dominguez Crespo Hirata, Mario Hiroyuki Hirata
Summary: The study aims to investigate the genetic, epigenetic, and pharmacogenomic factors associated with familial hypercholesterolemia (FH) and polygenic hypercholesterolemia (PH) through various experimental methods to elucidate the pathophysiological mechanisms and improve diagnosis and treatment levels.
RESEARCH IN SOCIAL & ADMINISTRATIVE PHARMACY
(2021)
Article
Chemistry, Physical
Shiva Najafi, Ali Asghar Moshtaghie, Farshid Hassanzadeh, Hashem Nayeri, Elham Jafari
Summary: This study aimed to evaluate the inhibitory activity of several atorvastatin derivatives against the HMG-CoA reductase enzyme. The results showed that compound S3 exhibited significant inhibitory activity and had a high anti-hyperlipidemic effect. Therefore, S3 could be considered as an effective medicine in preventing atherosclerosis.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Article
Biochemistry & Molecular Biology
Yvonne Carius, Michael Hutter, Flora Kiss, Rita Bernhardt, C. Roy D. Lancaster
Summary: This study revealed the crystal structure of cytochrome P450 CYP106A1 from Priestia megaterium, which shows a rare sixth beta-sheet. Comparison with the previously studied CYP106A2 and docking studies with the substrate cortisol revealed a different orientation of the steroid molecule in the active sites, explaining the observed differences in substrate conversion and product formation by the two enzymes.
Article
Biochemistry & Molecular Biology
Yuan Gao, Jingjing Ma
Summary: This study found that the A503V polymorphism can affect the metabolism of tacrolimus catalyzed by CYP3A5, and may serve as a potential biomarker for tacrolimus treatment in transplantation recipients.
EXPERT OPINION ON DRUG METABOLISM & TOXICOLOGY
(2022)
Review
Pharmacology & Pharmacy
Georgia Ragia, Vangelis G. Manolopoulos
EUROPEAN JOURNAL OF CLINICAL PHARMACOLOGY
(2020)
Review
Pharmacology & Pharmacy
Georgia Ragia, Vangelis G. Manolopoulos
Article
Cardiac & Cardiovascular Systems
Marta Futema, Uma Ramaswami, Lukas Tichy, Martin P. Bogsrud, Kirsten B. Holven, Jeanine Roeters van Lennep, Albert Wiegman, Olivier S. Descamps, Anne De Leener, Elodie Fastre, Michal Vrablik, Tomas Freiberger, Harald Esterbauer, Hans Dieplinger, Susanne Greber-Platzer, Ana M. Medeiros, Mafalda Bourbon, Vasiliki Mollaki, Euridiki Drogari, Steve E. Humphries
Summary: The most common cause of familial hypercholesterolaemia in children from eight European countries was found to be LDLR mutation, with significant variations in the prevalence of APOB p.(Arg3527Gln) mutation across different countries. LDLR-FH was associated with higher concentrations of LDL-C and a family history of coronary heart disease compared to APOB-FH in children.
Article
Oncology
Maria Panagopoulou, Makrina Karaglani, Vangelis G. Manolopoulos, Ioannis Iliopoulos, Ioannis Tsamardinos, Ekaterini Chatzaki
Summary: The study revealed the important role of DNA methylation in breast cancer pathogenesis and constructed three high-performing gene signatures for the discrimination, identification and early diagnosis of breast cancer. The selected genes in the signatures were associated with breast cancer-related pathways, highlighting the significance of gene methylation events in breast carcinogenesis.
Article
Pharmacology & Pharmacy
Charalampia Ioannou, Georgia Ragia, Ioanna Balgkouranidou, Nikolaos Xenidis, Kyriakos Amarantidis, Triantafyllia Koukaki, Eirini Biziota, Stylianos Kakolyris, Vangelis G. Manolopoulos
Summary: The study showed that TYMS-TSER 3R/2R polymorphism was associated with the incidence of adverse events in female cancer patients, potentially implicating the regulation of TS expression.
Article
Virology
Bradley Ward, Jean Cyr Yombi, Jean-Luc Balligand, Patrice D. Cani, Jean-Francois Collet, Julien de Greef, Joseph P. Dewulf, Laurent Gatto, Vincent Haufroid, Sebastien Jodogne, Benoit Kabamba, Sebastien Pyr Dit Ruys, Didier Vertommen, Laure Elens, Leila Belkhir
Summary: This paper introduces the HYGIEIA project, which aims to study the molecular mechanisms and post-COVID conditions through a multi-omic approach and network medicine analysis. By analyzing high-throughput sequencing and mass-spectrometry data from multiple biological layers, meaningful discoveries are expected and can be translated into improvements in clinical practice.
Article
Infectious Diseases
Perrin Ngougni Pokem, Xavier Wittebole, Christine Collienne, Hector Rodriguez-Villalobos, Paul M. Tulkens, Laure Elens, Francoise Van Bambeke, Pierre-Francois Laterre
Summary: This study investigated the pharmacokinetics of temocillin in plasma and ascitic fluid, and performed dosing simulations. The current dosing regimen achieved the target concentration in plasma, but not in ascitic fluid. Adjusting the dosing regimen might allow for target concentration attainment in ascitic fluid, but further evaluation is needed for safety and efficacy.
Editorial Material
Pharmacology & Pharmacy
Georgia Ragia, Vangelis G. Manolopoulos
Summary: The pharmaco-omics revolution is expected to greatly improve drug discovery and individual response to pharmacotherapy in the future.
Article
Environmental Sciences
Georgia-Eirini Deligiannidou, Chrysoula Kyrgou, Evangelia Nena, Vangelis G. Manolopoulos, Eugenia Bezirtzoglou, Christos A. Kontogiorgis, Theodoros C. Constantinidis
Summary: This study aimed to evaluate the use of medicinal and aromatic plants (MAPs) in various health disorders among residents in Thrace, NE Greece, and found that factors such as sex, employment, education, and body mass index influenced the consumption of MAPs.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Pharmacology & Pharmacy
Gavriela Voulgaridou, Theodora Paraskeva, Georgia Ragia, Natalia Atzemian, Konstantina Portokallidou, George Kolios, Konstantinos Arvanitidis, Vangelis G. Manolopoulos
Summary: The clinical implementation of therapeutic drug monitoring (TDM) is declining in Greek public hospitals, and efforts are urgently needed to reverse this trend.
Article
Biochemistry & Molecular Biology
Michail Spathakis, Gesthimani Tarapatzi, Eirini Filidou, Leonidas Kandilogiannakis, Evangelos Karatzas, Paschalis Steiropoulos, Dimitrios Mikroulis, George M. Spyrou, Vangelis G. Manolopoulos, George Kolios, Konstantinos Arvanitidis
Summary: Niclosamide, a commonly used helminthicidic drug, has been shown to attenuate the abnormal expression of collagen and fibronectin induced by cytokine stimulation, suggesting its potential as a therapeutic agent against lung fibrosis.
Article
Biochemistry & Molecular Biology
Natalia Atzemian, Nikolas Dovrolis, Georgia Ragia, Konstantina Portokallidou, George Kolios, Vangelis G. Manolopoulos
Summary: This study identified key genes in AF using bioinformatics tools and provided insights into the molecular pathogenesis of AF. Five differentially expressed genes were found in AF patients, and several pathways related to the extracellular matrix, inflammation, and structural remodeling were revealed.
Article
Biotechnology & Applied Microbiology
Dimitra Anatolou, Nikolas Dovrolis, Georgia Ragia, George Kolios, Vangelis G. G. Manolopoulos
Summary: COVID-19 is a systemic disease that affects various tissues and organs, including the lungs. This study analyzed transcriptomics data from lung and whole blood samples of patients, and identified differentially expressed genes and miRNAs related to COVID-19. These findings offer new insights into the systems biology of COVID-19.
OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Vasiliki Mollaki
Summary: Organoid technology holds great promise in biomedicine and biotechnology, but also raises complex ethical challenges. This study analyzes the multifaceted ethical issues and recommends measures to ensure ethical use and application of the technology. Despite the challenges, it is concluded that there is a moral obligation to support organoid research to maximize benefits and contribute to future governance models in the field.
Meeting Abstract
Transplantation
Athanasios Roumeliotis, Stefanos Roumeliotis, Fotis Tsetsos, Marios Theodoridis, Konstantia Kantartzi, Vangelis Manolopoulos, Ploumis Pasadakis, Stylianos Panagoutsos
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2019)