Article
Chemistry, Applied
Alba M. Garcia-Lino, Alex Gomez-Gomez, Dafne Garcia-Mateos, Alvaro de la Fuente, Ana I. Alvarez, Oscar J. Pozo, Gracia Merino
Summary: ABCG2 is involved in the secretion of several compounds in milk, with higher levels of tryptophan-related compounds in wild-type mice compared to Abcg2(-/-) mice. The milk-to-plasma ratio of these compounds was also higher in dairy cows carrying the ABCG2 Y581S polymorphism compared to noncarrier animals. In vitro transport assays confirmed these findings and validated differences in the bovine protein variants.
Article
Nanoscience & Nanotechnology
Yan Zhu, Yanni Xu, Dong Han, Xiujin Zhang, Cheng Qin, Jing Liu, Lei Tian, Mengqi Xu, Yan Fang, Yang Zhang, Yabin Wang, Feng Cao
Summary: In this study, a scavenger receptors AI (SR-AI) targeted theranostic nanoparticles were constructed to target foam cells and atherosclerotic plaques via ABCA1 activation. The SAU-NPs showed more active targeting to plaque lesions compared to non-SR-AI targeted nanoparticles. SAU-NPs carrying 5242331 inhibited the transformation to foam cells and effectively reduced cholesterol deposition through activating the LXR & alpha;-ABCA1/ABCG1/SR-BI pathway.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2023)
Article
Biochemical Research Methods
Holly R. Smith, Emily H. Canessa, Runia Roy, Rita Spathis, Michel Shamoon Pour, Yetrib Hathout
Summary: This study used mass spectrometry to detect pathogen proteins in black-legged ticks, and found that these ticks carried various pathogens, including Lyme disease and Rickettsia. Analysis of individual ticks revealed the presence of different pathogens.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Yubing Dong, Yi Lin, Wanyu Liu, Wei Zhang, Yinong Jiang, Wei Song
Summary: The study investigated the lipid profile in hypertensive disorders of pregnancy (HDP) patients, finding significantly altered levels of plasma triglycerides, total cholesterol, LDL-C, and HDL-C compared to normotensive controls. ANP was shown to inhibit ABCA1 and ABCG1 expression at both mRNA and protein levels, leading to impaired cholesterol efflux, possibly explaining the decreased HDL-C levels in HDP patients.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemical Research Methods
Ryuichi Hirota, Zen-ichiro Katsuura, Naoki Momokawa, Hiroki Murakami, Satoru Watanabe, Takenori Ishida, Takeshi Ikeda, Hisakage Funabashi, Akio Kuroda
Summary: In this study, it was found that under specific conditions, a phosphite-dependent strain of Escherichia coli can generate escape mutants. The mutation occurs at position 210 of the transmembrane domain protein HtxC of the phosphorus compound transporter HtxBCDE. The replacement of a specific amino acid residue at this position can reduce the rate of escape mutant generation.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Chemistry, Analytical
Pelin Yildiz, Sureyya Ozcan
Summary: Recent advances in proteomics technologies have allowed for high-throughput analysis of thousands of proteins. In this study, the correlation between protein concentration and corresponding unique peptide responses was investigated under conventional proteolytic digestion conditions. The results indicate that reproducible peptide signals could be misleading in clinical studies and that peptide selection could significantly impact the protein level outcome. This study is the first to explore quantitative protein-peptide correlations in biological samples using all unique peptides representing the same protein, and it opens up a discussion on peptide-based proteomics.
Article
Biochemistry & Molecular Biology
Alexey S. Kononikhin, Alexander G. Brzhozovskiy, Anna E. Bugrova, Natalia V. Chebotareva, Natalia V. Zakharova, Savva Semenov, Anatoliy Vinogradov, Maria I. Indeykina, Sergey Moiseev, Irina M. Larina, Evgeny N. Nikolaev
Summary: Targeted quantitative urine proteome analysis by mass spectrometry (MS) is a promising tool for early diagnosis of chronic kidney disease (CKD) and can replace invasive biopsy.
Article
Plant Sciences
Fiza Liaquat, Muhammad Farooq Hussain Munis, Samiah Arif, Muhammad Aamir Manzoor, Urooj Haroon, Iftikhar Hussain Shah, Muhammad Ashraf, Hyun Seok Kim, Shengquan Che, Liu Qunlu
Summary: This study investigated the differential gene expression of Schima superba under Mn metal stress through transcriptomic analysis. The differentially expressed genes were found to be involved in defense, signaling pathways, oxidative burst, transcription factors, and stress responses. The results provide insights into the metal resistance mechanisms and contribute to the understanding of heavy metal tolerance and detoxification.
FRONTIERS IN PLANT SCIENCE
(2022)
Editorial Material
Cell Biology
Joseph E. Aslan
Summary: Proteomics tools have greatly contributed to our understanding of platelet biology, with the ability to identify, detect, and quantify protein-related details. The platelet proteome is composed of thousands of different proteins, and changes in this proteome can affect platelet function in health and disease. Challenges still remain in conducting, validating, and interpreting platelet proteomics experiments, but future studies using advanced proteomics technologies may shed more light on platelet-mediated mechanisms in human health and disease.
Article
Biochemistry & Molecular Biology
Yaolei Mi, Xue Cao, Xuewen Zhu, Weiqiang Chen, Xiangxiao Meng, Huihua Wan, Wei Sun, Sifan Wang, Shilin Chen
Summary: This study identified and characterized 113 ABC transporters in C. sativa, and proposed seven core transporters that may participate in cannabinoid transport. These candidate genes showed high correlation with cannabinoid biosynthetic pathway genes and content, and were highly expressed in cannabinoid biosynthesis and accumulation sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Biochemistry & Molecular Biology
Samiksha Kukal, Debleena Guin, Chitra Rawat, Shivangi Bora, Manish Kumar Mishra, Priya Sharma, Priyanka Rani Paul, Neha Kanojia, Gurpreet Kaur Grewal, Shrikant Kukreti, Luciano Saso, Ritushree Kukreti
Summary: ABCG2 is a widely distributed protein in different cell types that plays a crucial role in maintaining cellular homeostasis and protecting tissues against xenobiotic insults, but its expression is influenced by various pathophysiological conditions. Therapeutic interventions should focus on regulating levels of ABCG2 rather than directly inhibiting its function to avoid serious side effects.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Solene Marie, Irene Hernandez-Lozano, Oliver Langer, Nicolas Tournier
Summary: Hepatocyte transporters play a crucial role in the hepatobiliary elimination of various substances, influenced by different pathophysiological conditions and specific drugs; Tc-99m-mebrofenin has been used for hepatobiliary scintigraphy for years, but the specific transporters controlling its liver kinetics have only recently been characterized; Pharmacokinetic modeling offers a translational method to study transporter-mediated drug interactions, providing insights into liver function as a biomarker.
JOURNAL OF NUCLEAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Silpa Narayanan, Zhuo-Xun Wu, Jing-Quan Wang, Hansu Ma, Nikita Acharekar, Jagadish Koya, Sabesan Yoganathan, Shuo Fang, Zhe-Sheng Chen, Yihang Pan
Summary: In this study, GS-9973 showed efficacy in overcoming ABCG2-mediated multidrug resistance in non-small cell lung cancer cells. The drug reversed drug resistance by blocking ABCG2 efflux activity and downregulating ABCG2 expression at the protein level, suggesting it as a potential strategy to overcome MDR in cancers.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Nutrition & Dietetics
Yoon-Young Sung, Dong-Seon Kim
Summary: The study found that eggshell membrane (EM) exhibits antihyperuricemic effects by promoting renal urate excretion and regulating renal urate transporters, resulting in reduced serum uric acid levels in hyperuricemic rats.
Article
Pharmacology & Pharmacy
Louise Breuil, Nora Ziani, Sarah Leterrier, Gaelle Hugon, Fabien Caille, Viviane Bouilleret, Charles Truillet, Maud Goislard, Myriam El Biali, Martin Bauer, Oliver Langer, Sebastien Goutal, Nicolas Tournier
Summary: This study investigated the impact of CYP inducers and inhibitors on the brain and plasma kinetics of [C-11]metoclopramide using PET imaging. The results showed that CYP induction or inhibition had negligible effects on the plasma kinetics and metabolism of [C-11]metoclopramide, but ritonavir significantly increased brain penetration.
Article
Cell Biology
Hansjorg Schwertz, Jesse W. Rowley, Irina Portier, Elizabeth A. Middleton, Neal D. Tolley, Robert A. Campbell, Alicia S. Eustes, Karin Chen, Matthew T. Rondina
Summary: Platelets play a central role in host responses during sepsis, and abnormalities in autophagic functions in platelets during sepsis are partly due to reduced EPG5-LC3 interactions, regulated by TLR4 engagement, leading to the accumulation of autophagosomes.
Article
Biochemistry & Molecular Biology
Kenta Tezuka, Masayoshi Suzuki, Risa Sato, Shohei Kawarada, Tetsuya Terasaki, Yasuo Uchida
Summary: Blood-brain barrier (BBB) dysfunction is a fundamental cause of multiple sclerosis. In this study, protein expression at the BBB of experimental autoimmune encephalomyelitis (EAE) mice was comprehensively quantified using the SWATH method. The down-regulation of tight junction molecules and the up-regulation of molecules involved in cell infiltration and tight junction disruption, including ANXA2, were observed. Interestingly, daily administration of an ANXA2 inhibitor significantly suppressed the development of EAE in mice. These findings suggest that ANXA2 signaling plays an important role in the pathogenesis of EAE.
JOURNAL OF NEUROCHEMISTRY
(2022)
Article
Chemistry, Medicinal
Hinako Nagano, Seiryo Ogata, Shingo Ito, Takeshi Masuda, Sumio Ohtsuki
Summary: This study reveals the role of Podocalyxin (PODXL) in blood-brain barrier function. PODXL knockdown affects immune response and intracellular trafficking, and regulates the protein expression and efflux activity of ABCB1/MDR1.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Hinako Nagano, Shingo Ito, Takeshi Masuda, Sumio Ohtsuki
Summary: The study suggests that INSR-mediated signaling is involved in regulating the expression of functional proteins at the blood-brain barrier (BBB), contributing to BBB function maintenance. Changes in protein expressions, particularly those related to amyloid-beta peptides, in INSR knockdown cells may contribute to the development of cerebral amyloid angiopathy through the suppression of INSR-mediated signaling.
PHARMACEUTICAL RESEARCH
(2022)
Article
Oncology
Seiryo Ogata, Takeshi Masuda, Shingo Ito, Sumio Ohtsuki
Summary: Targeted proteomics is a method that quantifies target proteins using liquid chromatography-tandem mass spectrometry. It allows rapid development, simultaneous measurement of multiple proteins, and high-specificity detection of modifications. It can seamlessly transfer candidate cancer biomarker proteins from discovery to verification and validation, accelerating the cancer biomarker pipeline.
Article
Biochemistry & Molecular Biology
Yasuo Uchida, Hina Takeuchi, Ryohei Goto, Clemens Braun, Holger Fuchs, Naoki Ishiguro, Masaki Takao, Mitsutoshi Tano, Tetsuya Terasaki
Summary: The purpose of this study was to determine the absolute abundance of proteins at the human blood-arachnoid barrier (BAB) and compare with dogs and pigs. The results showed that there were 52 highly expressed proteins at the BAB in humans, and the differences between humans and dogs or pigs were within 3-fold for most proteins. These findings provide important insights into the physiological roles of the BAB and the differences between humans and animals.
JOURNAL OF NEUROCHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Kristiina M. Huttunen, Tetsuya Terasaki, Arto Urtti, Ahmed B. Montaser, Yasuo Uchida
Summary: One of the major challenges in CNS-drug development is the presence of barriers that prevent substances from entering the brain. This review discusses the expression profiles of transporters in these barriers and how they affect drug disposition in the brain. It also highlights the advancements in structural biology and computational resources that have improved our understanding of transporter dynamics and aided in the design of transporter-utilizing compounds.
PHARMACEUTICAL RESEARCH
(2022)
Article
Chemistry, Analytical
Takeshi Masuda, Yuma Inamori, Arisu Furukawa, Maki Yamahiro, Kazuki Momosaki, Chih-Hsiang Chang, Daiki Kobayashi, Hiroto Ohguchi, Yawara Kawano, Shingo Ito, Norie Araki, Shao-En Ong, Sumio Ohtsuki
Summary: This study presents a water droplet in-oil digestion (WinO) method for proteomic analysis in limited sample amounts. The method increases protein and peptide recovery by minimizing the contact area between the sample solution and the container. It has been successfully applied to single-cell proteomics and shows promise for its wide applicability and suitability.
ANALYTICAL CHEMISTRY
(2022)
Article
Medicine, Research & Experimental
Keita Kitamura, Ayaka Okamoto, Hanae Morio, Ryuto Isogai, Ryo Ito, Yoshiyuki Yamaura, Saki Izumi, Takafumi Komori, Shingo Ito, Sumio Ohtsuki, Hidetaka Akita, Tomomi Furihata
Summary: This study characterizes the ability of the hiMCS-BBB models to evaluate receptor-mediated transcytosis (RMT)-mediated blood-brain barrier (BBB) penetration properties of middle/macromolecules. The results show that the hiMCS-BBB models have the potential to provide valuable tools for exploring and identifying middle/macromolecules with excellent BBB permeability levels.
MOLECULAR PHARMACEUTICS
(2022)
Article
Biochemistry & Molecular Biology
Haruki Horiguchi, Tsuyoshi Kadomatsu, Shinsei Yumoto, Takeshi Masuda, Keishi Miyata, Shuji Yamamura, Michio Sato, Jun Morinaga, Sumio Ohtsuki, Hideo Baba, Toshiro Moroishi, Yuichi Oike
Summary: Tumor cell-derived ANGPTL2 promotes beta-catenin-driven intestinal tumorigenesis by upregulating OB-cadherin expression and blocking destruction complex-independent proteasomal degradation of beta-catenin proteins. It also leads to decreased cell surface integrin alpha 5 beta 1 expression.
Article
Pharmacology & Pharmacy
Toshiki Kurosawa, Yuma Tega, Yasuo Uchida, Kei Higuchi, Hidetsugu Tabata, Takaaki Sumiyoshi, Yoshiyuki Kubo, Tetsuya Terasaki, Yoshiharu Deguchi
Summary: The PICK method successfully identified TM7SF3 and LHFPL6 as candidate proteins for the H+/OC antiporter and their functional analysis confirmed their role as components of this antiporter.
Article
Chemistry, Multidisciplinary
Ayano Mori, Takeshi Masuda, Shingo Ito, Sumio Ohtsuki
Summary: This study comprehensively assessed the digestion efficiency and stability of tryptic peptides, identified optimal signature peptides for human hepatic transporters and membrane marker proteins, and provided reliable quantification of human hepatic transporters for integrated pharmacokinetic studies.
PHARMACEUTICAL RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Yuki Yamasaki, Takashi Moriwaki, Seiryo Ogata, Shingo Ito, Sumio Ohtsuki, Genki Minegishi, Satoshi Abe, Yumi Ohta, Kanako Kazuki, Kaoru Kobayashi, Yasuhiro Kazuki
Summary: This study developed a novel P-glycoprotein humanized mouse line carrying the 2677G>T mutation and evaluated its influence on the expression and function of P-glycoprotein at the blood-brain barrier. The results showed that the mutation did not alter the expression levels of P-glycoprotein but increased the brain penetration of P-glycoprotein substrates. This mutant mouse line provides a useful model for studying the impact of MDR1 gene mutations on P-glycoprotein at the blood-brain barrier.
PHARMACOGENETICS AND GENOMICS
(2022)
Article
Hematology
Hansjorg Schwertz, Elizabeth A. Middleton
Summary: Autophagy plays an important role in megakaryopoiesis and platelet function, contributing to cellular function and homeostasis. Autophagy in platelets is involved in platelet function and clinical implications, but further research is needed to fully understand its role.
THROMBOSIS RESEARCH
(2023)
Article
Cell Biology
Takayuki Hoshii, Sarah Perlee, Sota Kikuchi, Bahityar Rahmutulla, Masaki Fukuyo, Takeshi Masuda, Sumio Ohtsuki, Tomoyoshi Soga, Behnam Nabet, Atsushi Kaneda
Summary: The protein degrader technology was used in this study to degrade SETD1A and investigate its role in acute myeloid leukemia (AML) cells. The results showed that SETD1A degradation led to downregulation of DNA repair and heme biosynthesis pathways, and SETD1A was also found to be essential for maintaining mitochondrial respiration in AML cells. Furthermore, the study revealed a non-enzymatic role of SETD1A in transcriptional pause release, providing insight into the mechanism of RNA polymerase II (RNAPII) pausing and its function in cancer.
