Methods to Make Homogenous Antibody Drug Conjugates

N-Terminal Protein Modification by Substrate-Activated Reverse Proteolysis

(2014) Sandra Liebscher et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Antibody-Drug Conjugates: An Emerging Concept in Cancer Therapy

(2014) Ravi V. J. Chari et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Bridging Disulfides for Stable and Defined Antibody Drug Conjugates

(2014) George Badescu et al.   BIOCONJUGATE CHEMISTRY

Production of Site-Specific Antibody–Drug Conjugates Using Optimized Non-Natural Amino Acids in a Cell-Free Expression System

(2014) Erik S. Zimmerman et al.   BIOCONJUGATE CHEMISTRY

Site-Specific Antibody–Drug Conjugation through Glycoengineering

(2014) Qun Zhou et al.   BIOCONJUGATE CHEMISTRY

Chemoenzymatic Fc Glycosylation via Engineered Aldehyde Tags

(2014) Elizabeth L. Smith et al.   BIOCONJUGATE CHEMISTRY

Unnatural amino acids in novel antibody conjugates

(2014) Trevor J Hallam et al.   Future Medicinal Chemistry

Rapid Ligations with Equimolar Reactants in Water with the Potassium Acyltrifluoroborate (KAT) Amide Formation

(2014) Hidetoshi Noda et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Engineering toward a bacterial “endoplasmic reticulum” for the rapid expression of immunoglobulin proteins

(2014) Dan Groff et al.   mAbs

Site-specific antibody-drug conjugation through an engineered glycotransferase and a chemically reactive sugar

(2014) Zhongyu Zhu et al.   mAbs

Small Targeted Cytotoxics: Current State and Promises from DNA-Encoded Chemical Libraries

(2013) Nikolaus Krall et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Enzymatic Labeling of Proteins: Techniques and Approaches

(2013) Mohammad Rashidian et al.   BIOCONJUGATE CHEMISTRY

Mass Spectrometric Characterization of Transglutaminase Based Site-Specific Antibody–Drug Conjugates

(2013) Santiago E. Farias et al.   BIOCONJUGATE CHEMISTRY

SGN-CD33A: a novel CD33-targeting antibody-drug conjugate using a pyrrolobenzodiazepine dimer is active in models of drug-resistant AML

(2013) M. S. Kung Sutherland et al.   BLOOD

Location Matters: Site of Conjugation Modulates Stability and Pharmacokinetics of Antibody Drug Conjugates

(2013) Pavel Strop et al.   CHEMISTRY & BIOLOGY

Cell-free translation of peptides and proteins:from high throughput screening to clinical production

(2013) Christopher J Murray et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Antibody–drug conjugates: current status and future directions

(2013) Heidi L. Perez et al.   DRUG DISCOVERY TODAY

Rapid Cross-Metathesis for Reversible Protein Modifications via Chemical Access to Se-Allyl-selenocysteine in Proteins

(2013) Yuya A. Lin et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Ligand-Free Palladium-Mediated Site-Specific Protein Labeling Inside Gram-Negative Bacterial Pathogens

(2013) Jie Li et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

New Functional Handle for Use as a Self-Reporting Contrast and Delivery Agent in Nanomedicine

(2013) Mathew P. Robin et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Site-specific antibody drug conjugates for cancer therapy

(2013) Siler Panowski et al.   mAbs

Methods for site-specific drug conjugation to antibodies

(2013) Christopher R Behrens et al.   mAbs

Imaging Trans-Cellular Neurexin-Neuroligin Interactions by Enzymatic Probe Ligation

(2013) Daniel S. Liu et al.   PLoS One

Ligation of Expressed Protein α-Hydrazides via Genetic Incorporation of an α-Hydroxy Acid

(2012) Yi-Ming Li et al.   ACS Chemical Biology

Multiple-Site Labeling of Proteins with Unnatural Amino Acids

(2012) Karin V. Loscha et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Synthesis of Heterobifunctional Protein Fusions Using Copper-Free Click Chemistry and the Aldehyde Tag

(2012) Jason E. Hudak et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Local Unfolding Is Required for the Site-Specific Protein Modification by Transglutaminase

(2012) Barbara Spolaore et al.   BIOCHEMISTRY

Site-Specific Protein Modification Using Lipoic Acid Ligase and Bis-Aryl Hydrazone Formation

(2012) Justin D. Cohen et al.   CHEMBIOCHEM

Antibody-Drug Conjugates for the Treatment of Cancer

(2012) John A. Flygare et al.   Chemical Biology & Drug Design

Streamlined Expressed Protein Ligation Using Split Inteins

(2012) Miquel Vila-Perelló et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

The discovery and development of brentuximab vedotin for use in relapsed Hodgkin lymphoma and systemic anaplastic large cell lymphoma

(2012) Peter D Senter et al.   NATURE BIOTECHNOLOGY

Conjugation site modulates the in vivo stability and therapeutic activity of antibody-drug conjugates

(2012) Ben-Quan Shen et al.   NATURE BIOTECHNOLOGY

Site-specific chemical protein conjugation using genetically encoded aldehyde tags

(2012) David Rabuka et al.   Nature Protocols

A Pictet-Spengler ligation for protein chemical modification

(2012) P. Agarwal et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Synthesis of site-specific antibody-drug conjugates using unnatural amino acids

(2012) J. Y. Axup et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Enzymatic Antibody Tagging: Toward a Universal Biocompatible Targeting Tool

(2012) Hang T. Ta et al.   TRENDS IN CARDIOVASCULAR MEDICINE

SNAP-Tag Technology Mediates Site Specific Conjugation of Antibody Fragments with a Photosensitizer and Improves Target Specific Phototoxicity in Tumor Cells

(2011) Ahmad Fawzi Hussain et al.   BIOCONJUGATE CHEMISTRY

Engineering of an anti-epidermal growth factor receptor antibody to single chain format and labeling by sortase A-mediated protein ligation

(2011) Mariusz P. Madej et al.   BIOTECHNOLOGY AND BIOENGINEERING

Microscale to manufacturing scale-up of cell-free cytokine production-a new approach for shortening protein production development timelines

(2011) James F. Zawada et al.   BIOTECHNOLOGY AND BIOENGINEERING

Site-specific protein labeling with amine-containing molecules using Lactobacillus plantarum sortase

(2011) Takuya Matsumoto et al.   Biotechnology Journal

Site-specific modification of ED-B-targeting antibody using intein-fusion technology

(2011) Sina Möhlmann et al.   BMC BIOTECHNOLOGY

In vitro Sortagging of an Antibody Fab Fragment: Overcoming Unproductive Reactions of Sortase with Water and Lysine Side Chains

(2011) Sina Möhlmann et al.   CHEMBIOCHEM

Palladium-mediated site-selective Suzuki–Miyaura protein modification at genetically encoded aryl halides

(2011) Christopher D. Spicer et al.   CHEMICAL COMMUNICATIONS

Trastuzumab Emtansine: A Unique Antibody-Drug Conjugate in Development for Human Epidermal Growth Factor Receptor 2-Positive Cancer

(2011) P. M. LoRusso et al.   CLINICAL CANCER RESEARCH

Brentuximab Vedotin (SGN-35)

(2011) J. Katz et al.   CLINICAL CANCER RESEARCH

Copper-Free Sonogashira Cross-Coupling for Functionalization of Alkyne-Encoded Proteins in Aqueous Medium and in Bacterial Cells

(2011) Nan Li et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Diels–Alder Cycloaddition for Fluorophore Targeting to Specific Proteins inside Living Cells

(2011) Daniel S. Liu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites

(2011) David B F Johnson et al.   Nature Chemical Biology

A general strategy for the evolution of bond-forming enzymes using yeast display

(2011) I. Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Site-Specific and Stoichiometric Modification of Antibodies by Bacterial Transglutaminase

(2010) Simone Jeger et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Adding New Chemistries to the Genetic Code

(2010) Chang C. Liu et al.   Annual Review of Biochemistry

Synthesis of an Acyltrifluoroborate and Its Fusion with Azides To Form Amides

(2010) Gary A. Molander et al.   JOURNAL OF ORGANIC CHEMISTRY

Olefin Cross-Metathesis on Proteins: Investigation of Allylic Chalcogen Effects and Guiding Principles in Metathesis Partner Selection

(2010) Yuya A. Lin et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A fluorophore ligase for site-specific protein labeling inside living cells

(2010) C. Uttamapinant et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Molecularly Defined Antibody Conjugation through a Selenocysteine Interface

(2009) Thomas Hofer et al.   BIOCHEMISTRY

A flexizyme that selectively charges amino acids activated by a water-friendly leaving group

(2009) Nobuyoshi Niwa et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Sortase-Mediated Ligation: A Gift from Gram-Positive Bacteria to Protein Engineering

(2009) Shinya Tsukiji et al.   CHEMBIOCHEM

Translation Initiation with Initiator tRNA Charged with Exotic Peptides

(2009) Yuki Goto et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Yeast Display Evolution of a Kinetically Efficient 13-Amino Acid Substrate for Lipoic Acid Ligase

(2009) Sujiet Puthenveetil et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases

(2008) Takahito Mukai et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Olefin metathesis for chemical biology

(2008) Joseph B Binder et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Rapid identification of reactive cysteine residues for site-specific labeling of antibody-Fabs

(2008) Jagath R. Junutula et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Allyl Sulfides Are Privileged Substrates in Aqueous Cross-Metathesis: Application to Site-Selective Protein Modification

(2008) Yuya A. Lin et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Site-specific conjugation of a cytotoxic drug to an antibody improves the therapeutic index

(2008) Jagath R Junutula et al.   NATURE BIOTECHNOLOGY

Targeted Cancer Therapy: Conferring Specificity to Cytotoxic Drugs

(2007) Ravi V. J. Chari   ACCOUNTS OF CHEMICAL RESEARCH