Article
Biochemistry & Molecular Biology
Adrienn Jenei, Gergo Kallo, Zsolt Dajnoki, Krisztian Gaspar, Andrea Szegedi, Aniko Kapitany, Eva Csosz
Summary: This study developed a novel approach for examining AMPs in the outer layer of the epidermis, with hexane-free processing producing higher protein yield. Among the investigated peptides, 9 could be detected in healthy or inflamed skin, and significant topographical differences were observed in the amount of certain peptides among different regions of healthy skin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Sagar Shukla, Annette L. Bunge, Hazem E. Hassan, Audra L. Stinchcomb
Summary: The study evaluated the impact of different investigators in studies using a tape stripping protocol to minimize investigator variability. The results showed that the measurements of different investigators are reproducible within the limitations of measurement variability, which can be managed by increasing volunteer numbers.
PHARMACEUTICAL RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Konstantin Simon, Gila Oberender, Alexander Roloff
Summary: Tape stripping is a common method used in studies on drug penetration through the skin, but the amount of stratum corneum removed by each tape strip is still under debate. Some studies suggest that the amount of stratum corneum decreases with increasing depth, while others find a constant removal rate.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Review
Allergy
F. L. de Boer, H. F. van Der Molen, S. Kezic
Summary: Dysfunction of the skin barrier is crucial in inflammatory skin diseases, and epidermal biomarkers can aid in evaluating its functionality. This review provides an overview of recent studies on epidermal biomarkers associated with the skin barrier's function and integrity and their application in research on atopic dermatitis and contact dermatitis. Potential biomarkers include lipids, natural moisturizing factors, structural proteins, corneocyte surface topography, and elasticity. While these biomarkers show promise, their validation for specific diseases is still needed.
CONTACT DERMATITIS
(2023)
Article
Pharmacology & Pharmacy
Mihaela Gorcea, Majella E. Lane, David J. Moore
Summary: The purpose of this in vivo exploratory study was to investigate the conformational order and chain packing of human stratum corneum lipids in healthy face skin as a function of depth. The study found that the lipid order of the stratum corneum increases with depth in face skin, but shows significant differences compared to arm skin.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Medicine, General & Internal
Ayano Maruyama, Risa Tamagawa-Mineoka, Hiromi Nishigaki, Koji Masuda, Norito Katoh
Summary: This is an important study exploring potential local and systemic biomarkers of AD, which will have clinical significance in discovering and assessing new biomarkers for AD.
Article
Dermatology
Masataka Ota, Takashi Sasaki, Tamotsu Ebihara, Emiko Yokosawa, Yumi Murakami, Hiroshi Matsunaka, Yuko Chinuki, Masayuki Amagai, Eishin Morita
Summary: The study found that loss-of-function mutations of the filaggrin (FLG) gene do not affect the severity of atopic dermatitis (AD) or skin barrier function, and have little impact on the amount of FLG protein and total amino acids in the stratum corneum of lesional skin. The results suggest that the production of FLG in skin lesions is influenced by the activation of Th2-dominant inflammatory cells along with FLG abnormalities.
JOURNAL OF DERMATOLOGY
(2021)
Article
Pharmacology & Pharmacy
Eleonora Maretti, Eliana Leo, Cecilia Rustichelli, Eleonora Truzzi, Cristina Siligardi, Valentina Iannuccelli
Summary: This study investigated the use of Nanostructured Lipid Carriers (NLC) to enhance skin permeation of the highly lipophilic beta-carotene (BC) for improved antioxidant effects. The smallest sized NLC sample showed deeper penetration into the skin barrier, potentially providing better protection against oxidative damages.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Dermatology
Mads Almose Ropke, Anna Mekulova, Christian Pipper, Maigi Eisen, Kristi Pender, Pieter Spee, Sanja Kezic
Summary: This study evaluated the use of the non-invasive patch technique by FibroTx for skin biomarker analysis in AD patients. The results demonstrated that this method can detect key biomarkers on lesional and non-lesional skin, which can reflect changes based on treatment. These findings support the effectiveness of using patch sampling for non-invasive assessment of inflammatory processes in AD skin.
SKIN RESEARCH AND TECHNOLOGY
(2021)
Article
Allergy
Susumu Murata, Sakae Kaneko, Eishin Morita
Summary: The improvement of dermatitis in atopic dermatitis (AD) patients is correlated with a decrease in stratum corneum interleukin-8 (scIL-8) levels after topical corticosteroid treatment. The reduction in scIL-8 levels is associated with the improvement in local skin severity in AD, indicating that scIL-8 may be a suitable biomarker for monitoring therapeutic effects in AD patients.
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
(2021)
Review
Chemistry, Analytical
S. Bielfeldt, F. Bonnier, H. J. Byrne, I. Chourpa, Y. Dancik
Summary: The study of human skin is significant in various fields including dermatology, toxicology, pharmacology, and cosmetology. Current measurement methods do not provide chemically specific results regarding skin penetration and permeation. However, Raman microspectroscopy can serve as an alternative method for non-destructive, non-invasive, and chemically specific investigations.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Medicine, General & Internal
Lydia Costello, Kirsty Goncalves, Victoria Maltman, Nicole Barrett, Kous Shah, Alison Stephens, Tereasa Dicolandrea, Ilaria Ambrogio, Erica Hodgson, Stefan Przyborski
Summary: The study demonstrates a novel application of tape stripping to mimic barrier damage and inflammation associated with dry shaving. Comparing it to razor-based shaving in vitro, it shows the potential of suitable shave- and skin-care formulations to mitigate damage. This provides a new methodology to examine grooming associated damage and a platform for screening potential skin care formulations.
FRONTIERS IN MEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Andrea Lo Presti, Nerio Andres Montoya, Valeria Criscuolo, Gulaly Khan, Usman Khan, Raffaele Vecchione, Christian Falconi
Summary: A theoretical framework is established for analyzing the impedances of multilayered tissues, enabling non-invasive characterizations of tissues beyond the stratum corneum. The results can advance the development of bioimpedance systems for the characterization of viable skin and adipose tissues in several applications.
ADVANCED MATERIALS
(2023)
Article
Pharmacology & Pharmacy
Stephanye Carolyne Christino Chagas, Camila de Almeida Perez Pimenta, Juliana Kishishita, Irla Carla Franca Barbosa, Danilo Cesar Galindo Bedor, Katia Aparecida da Silva Aquino, Davi Pereira de Santana, Leila Bastos Leal
Summary: Bioequivalence assessment of topical drug products has been a long-standing challenge. Different approaches have been proposed by regulatory agencies such as FDA and EMA, including the discussion of a Topical Classification System (TCS). This study used in vitro and in vivo BE approaches to evaluate mupirocin ointments marketed in Brazil, demonstrating their capability to distinguish among different formulations.
BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Multidisciplinary Sciences
Hye-Jin Ahn, Hae Jin Kim, Hyein Ham, Ji Hwoon Baek, Young Lee, Mahin Alamgir, Babar Rao, Min Kyung Shin
Summary: Objective assessments to evaluate sensitive skin do not well correlate with symptoms. Reflectance confocal microscopy (RCM) was used to compare transepidermal application of zinc in sensitive and non-sensitive skin, showing deeper and higher zinc reflectance in sensitive skin with visualized structural differences. RCM is suggested as a useful tool for evaluating skin barrier integrity.
SCIENTIFIC REPORTS
(2021)
Article
Pharmacology & Pharmacy
Philip A. Hines, Richard H. Guy, Angela Brand, Anthony J. Humphreys, Marisa Papaluca-Amati
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2020)
Article
Pharmacology & Pharmacy
M. Hoppel, M. A. M. Tabosa, A. L. Bunge, M. B. Delgado-Charro, R. H. Guy
Summary: This study successfully tested the input rates of nicotine and lidocaine into healthy human skin using tape-stripping method, revealing differences in drug delivery between transdermal patches and creams. The experimental approach was validated and confirmed using in vitro diffusion cells with pig skin samples.
Article
Medicine, Research & Experimental
Andrea Pensado, Laura Hattam, K. A. Jane White, Anita McGrogan, Annette L. Bunge, Richard H. Guy, M. Begona Delgado-Charro
Summary: Prediction of skin absorption and local bioavailability from topical formulations remains a challenging task, with current research focusing on developing a physiologically based pharmacokinetic model to predict distribution and absorption of complex dermatological products. In vitro experimental results were consistent with commercial product performance and new skin sampling data demonstrated the input rate and drug delivery requirements of the Scopoderm patch.
MOLECULAR PHARMACEUTICS
(2021)
Article
Pharmacology & Pharmacy
M. Alice Maciel Tabosa, Sarah F. Cordery, K. A. Jane White, Annette L. Bunge, Richard H. Guy, M. Begona Delgado-Charro
Summary: The role of excipients in the partitioning and diffusion of drugs in the skin is crucial in the development of dermatological drug products. This study correlated the skin pharmacokinetics of diclofenac and the co-uptake of two key excipients, propylene glycol and butylene glycol, in human subjects. The results highlight the importance of considering the disposition of excipients in the optimization of drug delivery from topical formulations.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Instruments & Instrumentation
Andrea Pensado, Anita McGrogan, K. A. Jane White, Annette L. Bunge, Richard H. Guy, M. Begona Delgado-Charro
Summary: Predicting the dermal bioavailability of topically delivered drugs is challenging. The study found that the uptake of betamethasone valerate into the stratum corneum was significantly higher at a dose of 5 mg/cm² compared to doses of 2 and 10 mg/cm². Skin blanching responses varied widely and were not sensitive to the dose of cream applied. The tape-stripping method was able to detect differences between applied doses, providing quantitative metrics for corticosteroid delivery.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Maxim Morin, Sebastian Bjorklund, Skaidre Jankovskaja, Kieran Moore, Maria Begona Delgado-Charro, Tautgirdas Ruzgas, Richard H. Guy, Johan Engblom
Summary: Non-invasive methods for early diagnosis of skin cancer are important. This study examined the use of reverse iontophoresis (RI) to enhance the extraction of tryptophan (Trp) and kynurenine (Kyn) for non-invasive sampling. The results showed that the Trp/Kyn ratio obtained from the skin surface matched that in the epidermal tissue. Increasing the pH of the receiver solution improved extraction efficiency, and using a bicontinuous cubic liquid crystal as the receiver medium showed comparable results to simple buffer solutions. Further in vivo investigations are warranted.
Correction
Medicine, Research & Experimental
Andrea Pensado, Laura Hattam, K. A. Jane White, Anita McGrogan, Annette L. Bunge, Richard H. Guy, M. Begon Delgado-Charro
MOLECULAR PHARMACEUTICS
(2022)
Article
Medicine, Research & Experimental
Hazel Garvie-Cook, Magdalena Hoppel, Richard H. Guy
Summary: This article illustrates the application of confocal Raman microspectroscopy and imaging to investigate the performance of topical and transdermal drug products at the formulation-skin interface. These techniques provide qualitative and potentially semi-quantitative insights into the interaction between the delivery system and the skin, drug transfer mechanisms, changes in drug solubility, and drug absorption and clearance within the skin.
MOLECULAR PHARMACEUTICS
(2022)
Article
Dermatology
O. Yelamos, D. Andersen, M. Pont, P. Iglesias, M. Potrony, M. Dominguez, A. Herrero, B. Alejo, J. Mateu, M. Ropke, N. B. Danneskiold-Samsoe, J. Malvehy, R. H. Guy, S. Brix, S. Puig
Summary: We developed a minimally invasive and image-guided tape stripping method for sampling atopic skin in children. This method is safe, reliable, and provides reproducible acquisition of cDNA. By comparing and optimizing skin tape stripping procedures using multiple imaging techniques, we successfully established a protocol that produced good cDNA yield without significant adverse events in a shorter time compared to previous methods. This minimally invasive method enables rapid sample collection, which is crucial in clinical practice.
CLINICAL AND EXPERIMENTAL DERMATOLOGY
(2023)
Article
Medicine, Research & Experimental
M. Alice Maciel Tabosa, Pauline Vitry, Panagiota Zarmpi, Annette L. Bunge, Natalie A. Belsey, Dimitrios Tsikritsis, Timothy J. Woodman, K. A. Jane White, M. Begona Delgado-Charro, Richard H. Guy
Summary: The evaluation of drug bioavailability in the skin requires quantitative and non-invasive experimental tools. In this study, infrared and Raman spectroscopies were used to assess the uptake of a chemical into the stratum corneum. Experiments on excised porcine skin showed good correlations between spectroscopic results and chemical quantification using the tape-stripping method. The spectroscopic approach can potentially be used to investigate chemical disposition deeper in the skin.
MOLECULAR PHARMACEUTICS
(2023)
Article
Biochemical Research Methods
Joan Eilstein, Vimal Nair, Kieran Moore, Steve Thomas Pannakal, Sebastien Gregoire, Prashant Ekhar, Richard H. Guy, M. Begona Delgado-Charro, Nita Roy
Summary: To commercially isolate phytochemicals, it is necessary to identify a suitable plant biomass source and conduct frequent analytical verification. Reverse iontophoretic (RI) sampling offers a potential solution that allows for non-destructive extraction of phytochemicals from different biomasses.
PHYTOCHEMICAL ANALYSIS
(2023)
Article
Medicine, Research & Experimental
Panagiota Zarmpi, M. Alice Maciel Tabosa, Pauline Vitry, Annette L. Bunge, Natalie A. Belsey, Dimitrios Tsikritsis, Timothy J. Woodman, M. Begona Delgado-Charro, Richard H. Guy
Summary: Confocal Raman spectroscopy is a promising tool for quantifying drug uptake and clearance in the skin, providing valuable insights into local bioavailability. This research confirmed that Raman signals can be used to correct for signal attenuation and detect drug disposition, as well as distinguish between different formulations.
MOLECULAR PHARMACEUTICS
(2023)
Article
Chemistry, Multidisciplinary
Kieran Moore, Shaun B. B. Reeksting, Vimal Nair, Steve T. T. Pannakal, Nita Roy, Joan Eilstein, Sebastien Gregoire, M. Begona Delgado-Charro, Richard H. H. Guy
Summary: Reverse iontophoresis (RI) was employed to non-destructively extract phytochemicals from intact fruits, and the extraction of ellagic acid from pomegranate fruit was successfully demonstrated. The RI sampling protocol was optimized, and multiple additional phytochemicals were also identified by LC-MS/MS.
Correction
Medicine, Research & Experimental
Andrea Pensado, Laura Hattam, K. A. . Jane White, Anita McGrogan, Annette L. Bunge, Richard H. Guy, M. Begona Delgado-Charro
MOLECULAR PHARMACEUTICS
(2022)
Article
Instruments & Instrumentation
Maria Alice Maciel Tabosa, Magdalena Hoppel, Annette L. Bunge, Richard H. Guy, M. Begona Delgado-Charro
Summary: The study shows that drug clearance from the difficult-to-access compartment in the skin can be inferred from systemic pharmacokinetic data, and is closely related to key physical chemical properties of the drug. A multiple regression analysis was used to determine this relationship and demonstrated that the model predictions correlated well with in vitro skin experiments.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2021)