Journal
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
Volume 466, Issue 5, Pages 973-985Publisher
SPRINGER
DOI: 10.1007/s00424-013-1346-5
Keywords
Calcium homeostasis; Excitation-contraction coupling; Ryanodine receptor; Sarcoplasmic reticulum Ca2+ release; Phosphatidylinositol phosphate
Categories
Funding
- Centre National de la Recherche Scientifique (CNRS)
- Universite Lyon 1
- Association Francaise contre les Myopathies (AFM)
- Hubert Curien Partnership Balaton [TeT_10-1-2011-0723]
- OTKA [K107765]
- Spanish Ministry of Education and Science (MEC)
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Skeletal muscle excitation-contraction (E-C) coupling is altered in several models of phosphatidylinositol phosphate (PtdInsP) phosphatase deficiency and ryanodine receptor activity measured in vitro was reported to be affected by certain PtdInsPs, thus prompting investigation of the physiological role of PtdInsPs in E-C coupling. We measured intracellular Ca2+ transients in voltage-clamped mouse muscle fibres microinjected with a solution containing a PtdInsP substrate (PtdIns(3,5)P (2) or PtdIns(3)P) or product (PtdIns(5)P or PtdIns) of the myotubularin phosphatase MTM1. No significant change was observed in the presence of either PtdIns(5)P or PtdIns but peak SR Ca2+ release was depressed by similar to 30% and 50% in fibres injected with PtdIns(3,5)P (2) and PtdIns(3)P, respectively, with no concurrent alteration in the membrane current signals associated with the DHPR function as well as in the voltage dependence of Ca2+ release inactivation. In permeabilized muscle fibres, the frequency of spontaneous Ca2+ release events was depressed in the presence of the three tested phosphorylated forms of PtdInsP with PtdIns(3,5)P (2) being the most effective, leading to an almost complete disappearance of Ca2+ release events. Results support the possibility that pathological accumulation of MTM1 substrates may acutely depress ryanodine receptor-mediated Ca2+ release. Overexpression of a mCherry-tagged form of MTM1 in muscle fibres revealed a striated pattern consistent with the triadic area. Ca2+ release remained although unaffected by MTM1 overexpression and was also unaffected by the PtdIns-3-kinase inhibitor LY2940002, suggesting that the 3-phosphorylated PtdIns lipids active on voltage-activated Ca2+ release are inherently maintained at a low level, inefficient on Ca2+ release in normal conditions.
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