Article
Biotechnology & Applied Microbiology
Alexander Yermanos, Kai-Lin Hong, Andreas Agrafiotis, Jiami Han, Sarah Nadeau, Cecilia Valenzuela, Asli Azizoglu, Roy Ehling, Beichen Gao, Michael Spahr, Daniel Neumeier, Ching-Hsiang Chang, Andreas Dounas, Ezequiel Petrillo, Ina Nissen, Elodie Burcklen, Mirjam Feldkamp, Christian Beisel, Annette Oxenius, Miodrag Savic, Tanja Stadler, Fabian Rudolf, Sai T. Reddy
Summary: DeepSARS is a high-throughput platform that enables simultaneous diagnostic detection and genomic surveillance of SARS-CoV-2. It combines multiple techniques to achieve highly sensitive viral detection and capture genomic diversity and viral evolution.
Article
Microbiology
Adrienne E. Shapiro, Alaina M. Olson, Lara Kidoguchi, Xin Niu, Zinhle Ngcobo, Zanele P. Magcaba, Mduduzi W. Ngwane, Grant R. Whitman, Kris M. Weigel, Rachel C. Wood, Doug P. K. Wilson, Paul K. Drain, Gerard A. Cangelosi
Summary: This study evaluated the use of urine LAM and tongue swabs for the diagnosis of tuberculosis. The results showed that combined testing of urine LAM and tongue swabs was more sensitive than using either nonsputum sample alone. Participants with tongue swab-positive results had higher sputum signal strength, suggesting that tongue swabs may serve as a complementary nonsputum sample for TB diagnosis.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Microbiology
Florentina Dumitrescu, Catalina-Gabriela Pisoschi, Vlad Padureanu, Andreea Cristina Stoian, Livia Dragonu, Lucian Giubelan
Summary: A 31-year-old HIV-infected patient was hospitalized for dyspnea, cough with mucopurulent sputum, and asthenia in July 2021. He was diagnosed with Serratia liquefaciens pneumonia and acute respiratory failure. Despite initial unfavorable outcomes, initiation of antituberculosis therapy led to significant improvement and discharge.
Article
Infectious Diseases
Sarah Welby, Mickael Cargnel, Claude Saegerman
Summary: Despite eradication efforts, bovine tuberculosis remains a threat in Europe. A study in Belgium found discrepancies in the surveillance system's performance and proposed using interferon gamma and serological testing as alternative methods to improve cost effectiveness. The results emphasized the importance of specificity in surveillance performance.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Medicine, General & Internal
Liyan Mao, Sylvia M. LaCourse, Soyeon Kim, Chang Liu, Bo Ning, Duran Bao, Jia Fan, Christopher J. Lyon, Ziyong Sun, Sharon Nachman, Charles D. Mitchell, Tony Y. Hu
Summary: This study evaluated the use of serum CFP-10 signal for diagnosing tuberculosis in HIV-infected and HIV-uninfected infants, demonstrating high sensitivity and specificity for TB diagnosis. The serum CFP-10 signal showed potential utility for early TB detection and monitoring of anti-TB treatment responses in children.
Article
Public, Environmental & Occupational Health
Helena Huerga, Mathieu Bastard, Alex Vicent Lubega, Milcah Akinyi, Natalia Tamayo Antabak, Liesbet Ohler, Winnie Muyindike, Ivan Mugisha Taremwa, Rosanna Stewart, Claire Bossard, Nothando Nkosi, Zibusiso Ndlovu, Catherine Hewison, Turyahabwe Stavia, Gordon Okomo, Jeremiah Okari Ogoro, Jacqueline Ngozo, Mduduzi Mbatha, Couto Aleny, Stephen Wanjala, Mohammed Musoke, Daniel Atwine, Alexandra Ascorra, Elisa Ardizzoni, Martina Casenghi, Gabriella Ferlazzo, Lydia Nakiyingi, Ankur Gupta-Wright, Maryline Bonnet
Summary: This study aimed to compare the diagnostic accuracy of the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay with the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) in HIV-positive patients. The results showed that the sensitivity of FujiLAM was 60%, while AlereLAM was 40%. The specificity of FujiLAM was 87%, while AlereLAM was 86%. The study suggests that urine-based genetic testing methods have potential clinical application value for the diagnosis of tuberculosis in HIV-positive patients, but the accuracy differences between FujiLAM lot numbers need to be addressed.
LANCET GLOBAL HEALTH
(2023)
Article
Multidisciplinary Sciences
Shrikala Acharya, Prashant Deshpande, Edwin Sam Asirvatham, Amol Palkar, Charishma Jones Sarman, Chinmay Laxmeshwar, Maninder Singh Setia, Dhirubhai Rathod, Sagar Koli, Jayesh Dale, Vijay Yeldandi, Ramesh Allam, Reshu Agarwal, Sanjeev Verma, Sunita Upadhyaya, Melissa Nyendak
Summary: The study demonstrated the utility and acceptability of using urine LF-LAM assay for TB diagnosis among AHD patients. The simple and user-friendly test can be included in the current diagnostic algorithm to facilitate early TB diagnosis among AHD patients.
Article
Microbiology
Mohammad Khaja Mafij Uddin, Md. Fahim Ather, Senjuti Kabir, Arfatur Rahman, Sabrina Choudhury, Rumana Nasrin, Tanjina Rahman, S. M. Mazidur Rahman, Shahriar Ahmed, Sayera Banu
Summary: Accurate and appropriate diagnosis of extrapulmonary tuberculosis (EPTB) remains challenging. This study investigated the diagnostic performance of different methods for EPTB diagnosis and found that the Xpert MTB/RIF assay showed the highest sensitivity. Considering its short turnaround time and promising findings, the Xpert MTB/RIF assay should be integrated into national TB guidelines as a routine diagnostic test.
Article
Multidisciplinary Sciences
Sylvia M. LaCourse, Evans Seko, Rachel Wood, Wilfred Bundi, Gregory S. Ouma, Janet Agaya, Barbra A. Richardson, Grace John-Stewart, Steve Wandiga, Gerard A. Cangelosi
Summary: In this study conducted in Kenya, the diagnostic performance of oral swab analysis (OSA) for tuberculosis (TB) was evaluated. The results showed that the sensitivity of buccal swabs varied depending on the reference standard used, with a sensitivity of 65.0% for sputum Xpert/culture and 76.5% for sputum Xpert alone. The sensitivity of buccal swabs was higher among HIV-infected individuals, but further optimization and evaluation of its utility in different settings are needed.
Article
Multidisciplinary Sciences
Hellen Hiza, Jerry Hella, Ainhoa Arbues, Beatrice Magani, Mohamed Sasamalo, Sebastien Gagneux, Klaus Reither, Damien Portevin
Summary: The study evaluated a simplified T cell activation marker (TAM)-TB assay for real-time detection of tuberculosis in adults, showing high accuracy and unaffected by HIV status.
SCIENTIFIC REPORTS
(2021)
Editorial Material
Medicine, General & Internal
Aneta Kacprzak, Karina Oniszh, Regina Podlasin, Maria Marczak, Iwona Cielniak, Ewa Augustynowicz-Kopec, Witold Tomkowski, Monika Szturmowicz
Summary: Tuberculosis (TB) is the major cause of illness, hospitalization, and death in individuals living with HIV (PLWH). The decrease in CD4+ T-lymphocyte count during HIV infection increases the risk of active TB and atypical clinical and radiologic presentation. This case emphasizes the importance of screening for HIV in patients with newly diagnosed TB.
Article
Multidisciplinary Sciences
Belen Saavedra, Edson Mambuque, Neide Gomes, Dinis Nguenha, Rita Mabunda, Luis Faife, Ruben Langa, Shilzia Munguambe, Filomena Manjate, Anelsio Cossa, Lesley Scott, Alberto L. Garcia-Basteiro
Summary: This study evaluated the field use of Abbott RealTime MTB and Xpert MTB/RIF assays in Southern Mozambique among HIV-positive and TB presumptive cases. Results showed improved sensitivity but reduced specificity for the Abbott RT-MTB compared to the Xpert MTB/RIF.
SCIENTIFIC REPORTS
(2021)
Article
Pathology
Alaine Umubyeyi Nyaruhirira, Jerod N. Scholten, Mustapha Gidado, Pedro G. Suarez
Summary: This opinion paper explores the importance of leveraging diagnostics for COVID-19 while retaining diagnostics for other infectious diseases. Integrated laboratory systems are crucial for efficient testing and increasing access to critical diagnostics. However, diagnostic capacity for one infectious disease may displace another, highlighting the need for mapping laboratory networks and allocating resources appropriately.
JOURNAL OF MOLECULAR DIAGNOSTICS
(2022)
Article
Microbiology
Edwin Nuwagira, Kathy Huppler Hullsiek, Samuel Jjunju, Morris Rutakingirwa, John Kasibante, Kiiza Kandole Tadeo, Enock Kagimu, Lillian Tugume, Kenneth Ssebambulidde, Abdu K. Musubire, Ananta Bangdiwala, Conrad Muzoora, David B. Meya, David R. Boulware, Nathan C. Bahr, Fiona V. Creswell
Summary: This study evaluated the role of CSF lactate in diagnosing and predicting outcomes of TBM in HIV-positive individuals. High CSF lactate levels were found to be associated with the diagnosis of TBM, but not predictive of 2-week mortality. CSF lactate may be a useful tool in diagnosing TBM in the clinical setting.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Gina Faraci, Sung Yong Park, Tanzy M. T. Love, Michael P. Dube, Ha Youn Lee
Summary: HIV microdrop sequencing is a powerful tool for large-scale and high-precision HIV genomic surveillance. The study successfully identified individuals with recent infections and achieved high detection accuracies. Moreover, the analysis of chronic HIV infection confirmed the false recency rate and estimated the time since infection with high consistency.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Olanrewaju Oladimeji, Bamidele Paul Atiba, Felix Emeka Anyiam, Babatunde A. A. Odugbemi, Tolulope Afolaranmi, Ayuba Ibrahim Zoakah, C. Robert Horsburgh
Summary: A retrospective study of 2555 DR-TB patients in Nigeria found that the majority were male, and males aged 20-29 and HIV-positive males had a lower likelihood of MDR-TB, while males from the south-south region and those aged >=60 had a higher probability of DR-TB. Tailored interventions targeting women of childbearing potential, young and older men, are necessary strategies to reduce DR-TB in Nigeria.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2023)
Article
Multidisciplinary Sciences
Amos Buh, Raywat Deonandan, James Gomes, Alison Krentel, Olanrewaju Oladimeji, Sanni Yaya
Summary: This study aims to assess the prevalence and predictors of antiretroviral therapy (ART) non-adherence among people living with HIV (PLWH) in Cameroon. The results showed that age, education, and alcohol consumption were significantly associated with ART non-adherence. Overall, a high proportion of participants exhibited non-adherence to antiretroviral therapy.
Editorial Material
Surgery
Busisiwe Mrara, Olanrewaju Oladimeji
WORLD JOURNAL OF SURGERY
(2023)
Article
Health Care Sciences & Services
Victor Abiola Adepoju, Ademola Adelekan, Olanrewaju Oladimeji
Summary: This study examined the stocking and dispensing practices of anti-TB medications among patent medicine vendors and community pharmacists in Nigeria, and identified the determinants of these practices. The results showed that the number of apprentices was significantly associated with medication stocking. Therefore, it is recommended that capacity-building and regulatory efforts for these healthcare providers should consider including apprentices.
Article
Public, Environmental & Occupational Health
Sylvia K. Mahoto, Honore K. Mitonga, Olanrewaju Oladimeji
Summary: This study aimed to identify and explore the perceived barriers preventing healthcare workers in Zambezi region, Namibia from delivering smoking cessation counselling to patients. The study found barriers including lack of time, inadequate training, insufficient knowledge, lack of guidelines and educational materials, and lack of patient interest and adherence. Targeted interventions and improvement of healthcare workers' skills and knowledge are crucial.
JOURNAL OF PUBLIC HEALTH IN AFRICA
(2023)
Article
Public, Environmental & Occupational Health
Edward K. Ameyaw, Kenneth S. Adde, Jones A. Paintsil, Kwamena S. Dickson, Olanrewaju Oladimeji, Sanni Yaya
Summary: Early initiation of breastfeeding is positively associated with healthcare delivery, and this study found a significant correlation between health facility delivery and early initiation of breastfeeding in sub-Saharan Africa.
HEALTH SCIENCE REPORTS
(2023)
Article
Infectious Diseases
Muhammed Rabiu Sahal, Gaetan Senelle, Kevin La, Tukur Wada Panda, Dalha Wada Taura, Christophe Guyeux, Emmanuelle Cambau, Christophe Sola
Summary: In this article, an in-depth analysis is provided on the drug-resistance phenotypic characteristics of tuberculosis in Nigeria. The study also characterizes the genetic features of different lineages, such as L4, L5, and L6, through whole genome sequencing. The results indicate a high rate of drug-resistance in the L4.6.2.2 lineage and a diverse L5 lineage. The study highlights the importance of sequencing more tuberculosis genomes in Nigeria and West Africa for public health and academic reasons, as well as the need to gather historical knowledge on tuberculosis in the region.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Health Care Sciences & Services
Victor Abiola Adepoju, Olanrewaju Oladimeji
Summary: This study examined the socio-demographic profiles and health service utilization patterns of Patent Medicine Vendors (PMVs) and Community Pharmacists (CPs) in Nigeria. The findings showed significant differences in the utilization of STD services, services by age <15, and utilization by males. Men visited PMVs more, while CPs used more STI services and childhood visits.
Article
Medicine, General & Internal
Ezile Julie Ninise, Busisiwe Mrara, Olanrewaju Oladimeji
Summary: This study investigated ICU refusal rates, reasons for refusal, characteristics, and outcomes at a resource-constrained rural setting in South Africa. The most common reason for refusal was being considered too sick to benefit from ICU. Additionally, being too well and lack of resources were also contributing factors. The refused patients had a mortality rate of 47%.
CLINICS AND PRACTICE
(2023)
Article
Nursing
Bukelwa Green, Daniel Ter Goon, Tobeka Mtise, Olanrewaju Oladimeji
Summary: This study investigated the attitudes, level of knowledge, and practices of professional nurses working in critical care units in the Eastern Cape Province regarding organ donation. The results showed that more than half of the nurses had a positive attitude towards organ donation, but their practice readiness was inadequate. This study is of significant importance in improving the knowledge and practice levels of organ donation and meeting the needs of patients requiring organ transplantation.
Article
Infectious Diseases
Maureen Nokuthula Sibiya, Kelechi Elizabeth Oladimeji, Felix Emeka Anyiam, Olanrewaju Oladimeji
Summary: Amid the COVID-19 pandemic, preventive measures such as hand hygiene, social distancing, and mask-wearing have been crucial in mitigating its spread. This study examines the COVID-19 prevention practices among staff and students of Durban University of Technology in South Africa. The findings indicate that these preventive practices are influenced by socio-demographic factors.
INFECTIOUS DISEASE REPORTS
(2023)
Review
Microbiology
Olanrewaju Oladimeji, Yasir Othman, Kelechi Elizabeth Oladimeji, Bamidele Paul Atiba, Victor Abiola Adepoju, Babatunde Adeniran Odugbemi
Summary: This study investigated patterns of drug-resistant tuberculosis (DR-TB) in Nigeria across six geopolitical zones from 2010 to 2016. The study found an increasing trend in the prevalence of DR-TB, with patients who received multiple treatments and those in the Northeastern region being more likely to have DR-TB. These patterns should be considered by clinicians when deciding on optimal drug combinations for the treatment of DR-TB patients.
MICROBIOLOGY RESEARCH
(2022)
Article
Infectious Diseases
Victor Abiola Adepoju, Kelechi Elizabeth Oladimeji, Olusola Adedeji Adejumo, Oluwatoyin Elizabeth Adepoju, Ademola Adelekan, Olanrewaju Oladimeji
Summary: This study found significant gaps in knowledge of International Standards for Tuberculosis Care (ISTC) among private non-NTP providers in Nigeria. Being female and being a nurse were associated with lower TB knowledge scores, while having previous experience managing a large number of TB patients was associated with higher knowledge scores. These findings highlight the importance of tailored mentorship and TB education, particularly for nurses and female providers in private healthcare settings.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2022)
Article
Infectious Diseases
Olanrewaju Oladimeji, Kelechi Elizabeth Oladimeji, Mirabel Nanjoh, Lucas Banda, Olukayode Ademola Adeleke, Teke Apalata, Jabu Mbokazi, Francis Leonard Mpotte Hyera
Summary: This study investigates the factors influencing TB treatment outcomes in Southwest Nigeria. The results show that reactive HIV status, positive attitude of healthcare workers, excellent services offered at healthcare facilities, and few people seeking care are independent significant determinants of successful treatment outcome.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2022)
Article
Health Care Sciences & Services
Victor Abiola Adepoju, Olanrewaju Oladimeji, C. Robert Horsburgh
Summary: In many high TB burden countries, a large proportion of people seek healthcare in the private sector when they fall sick. The private-sector is often perceived to offer poorer-quality health services and contribute to gaps in TB notification and the spread of MDR-TB. Efforts have been made to expand TB services among private providers through public-private mix (PPM) initiatives. However, evaluating the performance of PPM programs solely based on the proportion of private-sector seeking healthcare and undernotification is not a perfect approach. It is suggested to establish a uniform definition of PPM and standardize reporting tools to track patients who initially seek care in the private sector but are ultimately notified publicly.