Article
Pharmacology & Pharmacy
Daichi Saito, Chihiro Suzuki, Saori Tanaka, Shigekuni Hosogi, Kotoku Kawaguchi, Shinji Asano, Shohta Okamoto, Makoto Yasuda, Shigeru Hirano, Toshio Inui, Yoshinori Marunaka, Takashi Nakahari
Summary: This study investigated the effect of Ambroxol (ABX) on airway ciliary beating. It was found that ABX increased ciliary beat frequency and ciliary bend distance by stimulating Ca2+ release and activation of CaV1.2 channels. These findings suggest that ABX can enhance mucociliary clearance by modulating the calcium signaling pathway.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Multidisciplinary Sciences
Yankun Lyu, Phung N. Thai, Lu Ren, Valeriy Timofeyev, Zhong Jian, Seojin Park, Kenneth S. Ginsburg, James Overton, Julie Bossuyt, Donald M. Bers, Ebenezer N. Yamoah, Ye Chen-Izu, Nipavan Chiamvimonvat, Xiao-Dong Zhang
Summary: This study investigates the regulation of pH(i) in cardiomyocytes and finds a correlation between cardiac contractions and pH(i) transients. The pH(i) transients are influenced by various factors, including pacing rate, transporters, buffering capacity, and β-adrenergic signaling. Mitochondrial activity plays a role in pH(i) regulation, and a decrease in pH(i) may act as a negative feedback to cardiac contractions.
Article
Biology
Anatoly Mikhailik, Tatyana Michurina, Krikor Dikranian, Stephen Hearn, Vladimir Maxakov, Saul S. Siller, Ken-Ichi Takemaru, Grigori Enikolopov, Natalia Peunova
Summary: This passage discusses the crucial role of nitric oxide in regulating the polarity and ciliary beat frequency of multiciliated cells in the airway, and how this impacts airway flow. It also suggests a potential explanation for the low levels of exhaled nitric oxide in ciliopathies due to deficiencies in NO availability in ciliated cells.
LIFE SCIENCE ALLIANCE
(2021)
Article
Biology
Han Xiao, Tao Zhang, Changjun Li, Yong Cao, Linfeng Wang, Huabin Chen, Shengcan Li, Changbiao Guan, Jianzhong Hu, Di Chen, Can Chen, Hongbin Lu
Summary: Proper mechanical stimulation enhances the migration of Prrx1(+) cells and the release of active TGF-beta 1, promoting the repair of rotator cuff enthesis injuries through ciliary TGF-beta signaling.
Article
Cell Biology
Roberto Berra-Romani, Ajelet Vargaz-Guadarrama, Josue Sanchez-Gomez, Nayeli Coyotl-Santiago, Efrain Hernandez-Arambide, Jose Everardo Avelino-Cruz, Mario Garcia-Carrasco, Monica Savio, Giorgia Pellavio, Umberto Laforenza, Alfredo Lagunas-Martinez, Francesco Moccia
Summary: Histamine plays a role in inflammation and remodeling of lung fibroblasts by regulating intracellular calcium levels. This study investigated the signaling pathways involved in histamine-induced calcium signals in WI-38 fetal lung fibroblasts.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Goetz Schmidt, Gerrit Rienas, Sabrina Mueller, Katrin Richter, Michael Sander, Christian Koch, Michael Henrich
Summary: Mucociliary clearance is a vital physiological mechanism that protects the lungs from pollution and infection. The combination of cafedrine and theodrenaline can cause an increase in intracellular calcium concentration in respiratory epithelial cells.
SCIENTIFIC REPORTS
(2023)
Article
Otorhinolaryngology
W. Behr, F. Horschke, A. Nastev, C. E. Mueller, J. U. Sommer, B. Folz, H. Li, U. W. Geisthoff, B. A. Stuck, R. Birk
Summary: Research showed that TXA reduces CBF in a time and concentration dependent manner, potentially impairing nasal epithelial function significantly. Therefore, frequent or regular topical nasal application of TXA should be closely monitored for nasal function, especially in patients with co-morbidities like chronic rhinosinusitis.
EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY
(2021)
Article
Materials Science, Multidisciplinary
Yashoda Chandorkar, Celine Bastard, Jacopo Di Russo, Tamas Haraszti, Laura De Laporte
Summary: By using a light-responsive hydrogel as an actuating substrate, we investigated the response of myoblast cells to mechanical actuation and found that actuation enhances cell proliferation and migration while reducing collagen and fibronectin deposition. These findings highlight the importance of controlling the actuation time and amplitude for myoblast proliferation and mechanotransduction.
APPLIED MATERIALS TODAY
(2022)
Article
Computer Science, Interdisciplinary Applications
Woo June Choi, Jung-Ki Yoon, Bjorn Paulson, Chang-Hoon Lee, Jae-Joon Yim, Jong-Il Kim, Jun Ki Kim
Summary: The new video microscopy technique combines motion-contrast imaging and image correlation to provide objective and selective ciliary beat frequency (CBF) measurements. The method's performance was validated with in vitro human airway organoid models, showing robustness to optical focal shifts and exhibiting spatial heterogeneity and temperature dependence.
IEEE TRANSACTIONS ON MEDICAL IMAGING
(2022)
Review
Biochemistry & Molecular Biology
Carmen Costas-Ferreira, Lilian R. F. Faro
Summary: Pesticides from different chemical classes exert toxic effects on the nervous system by altering calcium homeostasis, leading to oxidative stress, neuroinflammation, and ultimately neuronal death. Proposed strategies to counteract these effects are also discussed in the review.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Sebastian Mestril, Raehyun Kim, Samuel S. Hinman, Shawn M. Gomez, Nancy L. Allbritton
Summary: The study demonstrated that colonic epithelial cultures enriched for stem/proliferative cells exhibit higher frequency and shorter pulse width of Ca2+ transients, and differentiation leads to a decrease in transient frequency by 50% and an increase in pulse width by 200%. Various metabolites and growth factors can modulate Ca2+ oscillation patterns consistently, while increasing the stiffness of the supportive matrix shifts Ca2+ transient patterns toward those resembling differentiated cells.
ADVANCED HEALTHCARE MATERIALS
(2021)
Review
Chemistry, Multidisciplinary
Yonggang Zhang, Pamela Habibovic
Summary: Biochemical signals and biophysical cues both play crucial roles in regulating cellular activities and maintaining tissue and organ function. This article provides an overview of the current state of the art in designing and developing materials and devices for delivering mechanical stimuli to cells and tissues, highlighting challenges and perspectives for future research.
ADVANCED MATERIALS
(2022)
Review
Cell Biology
Eunhee Kim, Dong Min Lee, Min Ji Seo, Hong Jae Lee, Kyeong Sook Choi
Summary: Paraptosis is a type of programmed cell death characterized by dilation of the endoplasmic reticulum and/or mitochondria. Ca2+ transport has been shown to play an important role in paraptosis induced by various agents, and perturbations of cellular proteostasis and ion homeostasis are believed to contribute critically to the process. The communication between the ER and mitochondria mediated by Ca2+ appears to be crucial in inducing paraptosis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Beibei Wu, Jin Seok Woo, Pamela Vila, Marcus Jew, Jennifer Leung, Zuoming Sun, Sonal Srikanth, Yousang Gwack
Summary: In effector T cells, the pore subunit of CRAC channels, ORAI1, is limited in plasma membrane localization with a significant fraction trapped in intracellular vesicles. NKD2, an essential component of ORAI1(+) vesicles activated by signaling pathways downstream of T cell receptors, orchestrates trafficking and insertion of ORAI1(+) vesicles to the plasma membrane. T cell receptor (TCR)-stimulation-dependent insertion of ORAI1 into the plasma membrane is essential for sustained calcium signaling and cytokine production in T cells.
Article
Cell Biology
Fumiko Matsukawa Usami, Masaki Arata, Dongbo Shi, Sanae Oka, Yoko Higuchi, Fadel Tissir, Masatoshi Takeichi, Toshihiko Fujimori
Summary: CELSR1 and CAMSAP3 play critical roles in determining cilia orientation within and between cells, with their cooperation being essential for the generation of functional multi-ciliated tissues.
JOURNAL OF CELL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Yang Liu, Huiwen Wang, Jun Wang, Bin Wei, Xinyi Zhang, Mengqi Zhang, Dong Cao, Jiang Dai, Zhen Wang, Eric Nyirimigabo, Guangju Ji
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2019)
Article
Cardiac & Cardiovascular Systems
Fabian Belau, Katharina Metzner, Torsten Christ, Ursula Ravens, Michael Schaefer, Stephan Kuenzel, Wener Li, Erich Wettwer, Dobromir Dobrev, Ali El-Armouche, Susanne Kaemmerer
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2019)
Article
Cell Biology
Xiuyun Sun, Jun Wang, Xia Yao, Wen Zheng, Yang Mao, Tianlong Lan, Liguo Wang, Yonghui Sun, Xinyi Zhang, Qiuye Zao, Jianguo Zhao, Rui-Ping Xiao, Xiuqin Zhang, Guangju Ji, Yu Rao
Article
Materials Science, Multidisciplinary
Jiuye Chen, Wei Sun, Ning Liu, Qidong Wang, Yulong Wang, Guangju Ji, Fengyi Ke, Ding Ma, Huining Zhao
Article
Cell & Tissue Engineering
Wener Li, Xiaojing Luo, Ying Ulbricht, Michael Wagner, Christopher Piorkowski, Ali El-Armouche, Kaomei Guan
STEM CELL RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Suzan J. G. Knottnerus, Isabella Mengarelli, Rob C. Wust, Antonius Baartscheer, Jeannette C. Bleeker, Ruben Coronel, Sacha Ferdinandusse, Kaomei Guan, Lodewijk IJlst, Wener Li, Xiaojing Luo, Vincent M. Portero, Ying Ulbricht, Gepke Visser, Ronald J. A. Wanders, Frits A. Wijburg, Arie O. Verkerk, Riekelt H. Houtkooper, Connie R. Bezzina
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Cell Biology
Xiaojing Luo, Wener Li, Karolina Kuenzel, Sarah Henze, Lukas Cyganek, Anna Strano, Mareike S. Poetsch, Mario Schubert, Kaomei Guan
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Cell Biology
Wener Li, Michael Stauske, Xiaojing Luo, Stefan Wagner, Meike Vollrath, Carola S. Mehnert, Mario Schubert, Lukas Cyganek, Simin Chen, Sayed-Mohammad Hasheminasab, Gerald Wulf, Ali El-Armouche, Lars S. Maier, Gerd Hasenfuss, Kaomei Guan
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Cell & Tissue Engineering
Wener Li, Sarah Henze, Xiaojing Luo, Ying Ulbricht, Anja Richter, Nataliya Di Donato, Arthur A. M. Wilde, Kaomei Guan
Summary: Catecholaminergic polymorphic ventricular tachycardia (CPVT) is a severe inherited cardiac disorder caused mainly by mutations in the RYR2 gene. In this study, two induced pluripotent stem cell lines were successfully generated from a CPVT patient, providing a valuable tool for disease modeling and research.
STEM CELL RESEARCH
(2021)
Article
Cell & Tissue Engineering
Junling Gao, Juntao Yuan, Qun Liu, Yuanli Wang, Huiwen Wang, Yingjie Chen, Wenjun Ding, Guangju Ji, Zhongbing Lu
Summary: The research found that ADSCs can protect against the adverse health effects of PM2.5 exposure by attenuating pulmonary inflammation and cell death. This suggests that ADSC transplantation may be a potential therapeutic approach for severe air pollution-associated diseases.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Genetics & Heredity
Youkun Bi, Lei Gu, Jun Wang, Yan Chang, Meng Jin, Yang Mao, Huiwen Wang, Guangju Ji
Summary: The AdBlue system, utilizing an enzymatic assembly strategy, significantly reduces the time and labor required for constructing recombinant adenoviruses, simplifies the preparation of adenoviruses carrying nuclease complexes for CRISPR/Cas9 design, and induces high levels of site-specific mutagenesis.
Article
Cell Biology
Youkun Bi, Zhiguang Yang, Meng Jin, Kui Zhai, Jun Wang, Yang Mao, Yang Liu, Mingqin Ding, Huiwen Wang, Fengchao Wang, Hong Cai, Guangju Ji
Summary: This study demonstrates that the ERp44 gene plays a crucial role in the formation of endocardial cushions during heart development. It affects the development of the endocardial cushion by influencing VEGFA-mediated endocardial-mesenchymal transition.
CELL PROLIFERATION
(2022)
Article
Cell & Tissue Engineering
Wener Li, Xiaojing Luo, Ying Ulbricht, Kaomei Guan
Summary: This study improved the method for single iPSC-CM preparation by applying 2.5 μm blebbistatin throughout the procedure, leading to significant improvements in cell morphology and electrical recording. The automated patch-clamp technique accurately recapitulated the electrophysiological phenotypes of iPSC-CMs derived from Brugada syndrome patients, demonstrating its potential to replace manual patch-clamp in modeling cardiac channelopathies with higher throughput.
STEM CELL RESEARCH
(2021)
Article
Cell & Tissue Engineering
Youkun Bi, Xuejun Guo, Mengqi Zhang, Keqi Zhu, Chentao Shi, Baoqi Fan, Yanyun Wu, Zhiguang Yang, Guangju Ji
Summary: The study found that promoting mitochondrial transfer via stem cell transplantation can effectively treat NAFLD and related metabolic diseases by restoring dysfunctional mitochondria and reducing fat accumulation.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Chemistry, Medicinal
Wener Li, Xiaojing Luo, Mareike S. Poetsch, Reinhard Oertel, Kapil Nichani, Martin Schneider, Anna Strano, Marcel Hasse, Robert-Patrick Steiner, Lukas Cyganek, Karina Hettwer, Steffen Uhlig, Kirsten Simon, Kaomei Guan, Mario Schubert
Summary: In this study, human-induced pluripotent stem cell-derived cardiomyocytes were used to investigate the effects of hydroxychloroquine and azithromycin, as well as their combination, on the structure and functionality of cardiomyocytes. The results showed synergistic adverse effects of azithromycin and hydroxychloroquine on the electrophysiological and contractile function of cardiomyocytes, leading to higher cardiotoxicity and more severe structural and functional abnormalities.