Article
Environmental Sciences
Stefanie Reinold, Alicia Herrera, Francesco Saliu, Carlos Hernandez-Gonzalez, Ico Martinez, Marina Lasagni, May Gomez
Summary: The presence of microplastics in seafood, particularly in farmed fish, is a concerning issue as it poses potential risks to human health. A study conducted in Tenerife, Spain found that 65% of the examined fish cultivated in coastal waters contained microplastics in their gastrointestinal tracts. Fibres and fragments were the predominant shapes of microplastics detected, with varying compositions.
MARINE POLLUTION BULLETIN
(2021)
Article
Environmental Sciences
Agata Egea-Corbacho, Ana Pilar Martin-Garcia, Ana A. Franco, Gemma Albendin, Juana M. Arellano, Rocio Rodriguez-Barroso, M. Dolores Coello, Dolores Coello, Jose F. Cabello, Iria Iglesias Prado, Erik-jan Malta
Summary: This study investigates the impact of microplastics (MP) on aquaculture systems, covering water intake, purification treatment, culture tanks, feed, and the organism. The study shows that purification systems can reduce the entry of MP from the environment, but new MP are observed in the sea bass tank, mainly due to added feed and materials used in aquaculture systems. Different types of MP are found in the sea bass samples. Consideration and reduction of other entry routes are necessary to prevent MP from reaching the human consumer in aquaculture facilities.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Engineering, Marine
Mary E. M. Larkin, Allen R. Place
Summary: Incorporating environmentally sustainable plant-based protein sources into aquafeeds is important, but the effects of specific plant proteins on different fish species need to be evaluated. Previous studies showed that wheat gluten at less than 4% inclusion had a negative impact on the growth and survival rate of juvenile cobia. In this study, the effects of wheat gluten inclusion in a plant-based diet for juvenile European sea bass were evaluated, and it was found that 4% dietary wheat gluten was well tolerated by the fish.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2023)
Article
Immunology
Ines A. Ferreira, Diogo Peixoto, Ana Paula Losada, Maria Isabel Quiroga, Ana do Vale, Benjamin Costas
Summary: This study aimed to understand the early innate immune response of European sea bass to T. maritimum infection. The results showed that T. maritimum induced a local immune response in the skin, gills, and posterior-intestine of the fish. This may be due to the bacteria's ability to adhere, colonize, and damage these organs. The infected fish also exhibited systemic responses, including increased neutrophils, signs of anemia, and decreased bactericidal and lysozyme activities in the plasma.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Fisheries
Laura Sempere, Soledad Ibanez, Conrado Marin, Gregorio Moles, Sara Puchol, Jesus Rosel, Jaume Perez-Sanchez, Alicia Felip
Summary: This study compares the growth performance and reproductive physiology of 2-year-old female sea bass with early or late onset puberty. The study provides valuable information on the factors associated with early onset puberty in this species.
AQUACULTURE REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Marina Machado, Diogo Peixoto, Paulo Santos, Ana Ricardo, Ines Duarte, Ines Carvalho, Claudia Aragao, Rita Azeredo, Benjamin Costas
Summary: This study aimed to investigate the effects of dietary tryptophan supplementation on the immune condition of European seabass during stressful rearing conditions and their response to bacterial pathogens. The results showed that stress did not compromise the health indicators of seabass, but exacerbated the inflammatory response. Tryptophan had minimal effects on immune indicators but improved the inflammatory response against bacterial pathogens during stressful conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Helena Fernandes, Nicole Martins, Lucia Vieira, Jose Manuel Salgado, Carolina Castro, Aires Oliva-Teles, Isabel Belo, Helena Peres
Summary: The study found that solid-state fermentation (SSF) was the most effective processing method for Ulva rigida, as it improved feed efficiency without affecting the growth performance of fish.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Fisheries
S. Torrecillas, S. Rimoldi, D. Montero, A. Serradell, F. Acosta, R. Fontanillas, F. Allal, P. Haffray, A. Bajek, G. Terova
Summary: The study aimed to replace dietary fishmeal/fish oil without affecting European sea bass performance and health status. The study found that diet and genotype have a significant impact on the gut bacterial composition of sea bass, but without negative effects on their health and growth. Selectively bred sea bass had a more adaptable gut microbiota composition.
Article
Agriculture, Dairy & Animal Science
Charles Rodde, Hugues de Verdal, Marc Vandeputte, Francois Allal, Julie Nati, Mathieu Besson, Felipe R. Blasco, John A. H. Benzie, David J. McKenzie
Summary: The study investigated the prediction of individual FE in European sea bass, identifying significant effects of temperature and population on FE and RMR. However, individual FE and RMR were not significantly correlated, indicating a need for further investigation into the relationship between metabolic rate and feed efficiency.
JOURNAL OF ANIMAL SCIENCE
(2021)
Article
Environmental Sciences
Stephen Knobloch, Joris Philip, Sebastien Ferrari, David Benhaim, Martine Bertrand, Isabelle Poirier
Summary: This study examined the impact of ultrasound on farmed European sea bass and found that while growth and survival were not affected, ultrasound exposure led to disruption of gill and skin microbiota, including an increase in potential pathogenic bacteria. This highlights the need for further research on the long-term effects of ultrasonic antifouling control on the health and well-being of farmed fish.
MARINE POLLUTION BULLETIN
(2021)
Article
Environmental Sciences
Nicole Francesca Pelusio, Luca Parma, Enrico Volpe, Sara Ciulli, Francesca Errani, Silvia Natale, Alessandra De Cesare, Valentina Indio, Paolo Carcano, Oliviero Mordenti, Pier Paolo Gatta, Alessio Bonaldo
Summary: Functional ingredients, such as nucleotides and nucleic acids, have shown potential for improving fish meal aquafeed formulation in Mediterranean aquaculture. In a study on European sea bass juveniles, the addition of nucleotides and nucleic acids to the feed increased growth performance and enhanced lipid efficiency. These ingredients also had prebiotic effects on the gut microbiome, promoting beneficial bacteria. However, the optimal dosage and combination of nucleotides and nucleic acids in fish meal require further investigation.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Multidisciplinary Sciences
Claudia R. Serra, Aires Oliva-Teles, Paula Enes, Fernando Tavares
Summary: A healthy gastrointestinal microbiota is vital for host fitness and is strongly affected by host diet. Feeding carnivorous fish plant-based diets high in non-starch polysaccharides (NSP) can lead to changes in gut microbiota composition, favoring bacterial families with carbohydrate active enzymes. Overall, the gut communities of European seabass respond to plant-based diets by adjusting to the presence of transient microorganisms with carbohydrolytic potential while maintaining a balanced core microbiota.
SCIENTIFIC REPORTS
(2021)
Article
Fisheries
Carlos Orduna, Lourdes Encina, Amadora Rodriguez-Ruiz, Victoria Rodriguez-Sanchez
Summary: The study found that horizontal hydroacoustics is a useful technique for monitoring size distribution of sea bass in farming cages, with the most suitable sampling method being the use of a vertically moving transducer positioned outside the cage.
Article
Biotechnology & Applied Microbiology
Helena Fernandes, Jose Manuel Salgado, Marta Ferreira, Martina Vrsanska, Nelson Fernandes, Carolina Castro, Aires Oliva-Teles, Helena Peres, Isabel Belo
Summary: In this study, Brewer's spent grain (BSG) was fermented with Aspergillus ibericus to produce a crude extract rich in cellulase and xylanase. The fermented BSG showed increased protein content and reduced fiber content, making it a potential supplement in aquafeeds. The crude extract was also found to significantly improve feed and protein utilization in European seabass juveniles.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Marine
Ana Ramljak, Irena Vardic Smrzlic, Damir Kapetanovic, Fran Barac, Anamarija Kolda, Lorena Peric, Ivana Balenovic, Tin Klanjscek, Ana Gavrilovic
Summary: This study examined the culturable skin microbiota associated with farmed European seabass and found that the majority of bacteria on the skin belonged to the genera Pseudomonas and Vibrio. Some of the identified microbiota were pathogenic to fish and showed higher resistance to certain antibiotics. This study provides valuable information on the culturable skin bacteria of healthy European seabass under different antibiotic usage conditions and highlights the potential problems for fish health management.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2022)
Article
Environmental Sciences
Federico Moroni, Fernando Naya-Catala, M. Carla Piazzon, Simona Rimoldi, Josep Calduch-Giner, Alberto Giardini, Ines Martinez, Fabio Brambilla, Jaume Perez-Sanchez, Genciana Terova
Summary: The addition of nisin-producing Lactococcus lactis in gilthead sea bream had effects on growth performance, transcriptional response, and microbiota, without colonization in the host's intestinal mucosa. The probiotic in the diets modulated the gut microbiota of fish, leading to host modifications without necessarily colonizing the intestine mucosa.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Michela Castagna, Raffaella Cinquetti, Tiziano Verri, Francesca Vacca, Matteo Giovanola, Amilcare Barca, Tiziana Romanazzi, Cristina Roseti, Alessandra Galli, Elena Bossi
Summary: The SLC6 family includes 20 human transporters classified into neurotransmitter transporters and nutrient amino acid transporters. Insects' orthologs of SLC6 family transporters have served as important tools for investigating mammalian counterparts.
NEUROCHEMICAL RESEARCH
(2022)
Article
Clinical Neurology
Ludovica Iovino, Veronica Giusti, Francesca Pischedda, Elena Giusto, Nicoletta Plotegher, Antonella Marte, Ilaria Battisti, Angela Di Iacovo, Algerta Marku, Giovanni Piccoli, Rina Bandopadhyay, Carla Perego, Tiziana Bonifacino, Giambattista Bonanno, Cristina Roseti, Elena Bossi, Giorgio Arrigoni, Luigi Bubacco, Elisa Greggio, Sabine Hilfiker, Laura Civiero
Summary: This study demonstrates that the pathogenic variant G2019S of leucine-rich repeat kinase 2 (LRRK2) impairs the function of Excitatory Amino Acid Transporter 2 (EAAT2), resulting in glutamate overload in the brain, which may contribute to neurodegeneration in Parkinson's disease (PD).
ACTA NEUROPATHOLOGICA
(2022)
Article
Neurosciences
Francesca Vacca, Ana S. Gomes, Koji Murashita, Raffella Cinquetti, Cristina Roseti, Amilcare Barca, Ivar Ronnestad, Tiziano Verri, Elena Bossi
Summary: Two slc15a2-type genes were identified in the Atlantic salmon genome, encoding PepT2-type peptide transporters. While slc15a2a transcripts are widely distributed in fish tissues, abundant in the brain and gills, slc15a2b transcripts are mainly expressed in the kidney and distal gastrointestinal tract. Both PepT2a and PepT2b function as high-affinity transporters.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Review
Biochemistry & Molecular Biology
Manan Bhatt, Angela Di Iacovo, Tiziana Romanazzi, Cristina Roseti, Raffaella Cinquetti, Elena Bossi
Summary: The heterologous expression of proteins in Xenopus laevis oocytes remains essential in many research fields. New approaches and revised protocols are applied to study membrane transporters, and both new and classic methods for investigating the activity and expression of Solute Carriers (SLC) are reviewed.
Review
Pharmacology & Pharmacy
Manan Bhatt, Angela Di Iacovo, Tiziana Romanazzi, Cristina Roseti, Elena Bossi
Summary: The role and function of betaine in the brain have been little studied and reviewed, despite its well-documented effects in the liver and kidney. This review analyzes the beneficial effects of betaine in neurodegeneration, excitatory and inhibitory imbalance, and oxidative stress in the central nervous system. However, there are still many unknown aspects regarding its transport and the actions beyond the known protein, betaine-GABA transporter 1. This article highlights the lack of detailed molecular and cellular information and the importance of studying the interaction of betaine with CNS components for its therapeutic use.
BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Antonio Serradell, Daniel Montero, Alvaro Fernandez-Montero, Genciana Terova, Alex Makol, Victoria Valdenegro, Felix Acosta, Maria Soledad Izquierdo, Silvia Torrecillas
Summary: The study evaluates the potential of two functional additives (GMOS and PHYTO) as boosters of gill endogenous antioxidant capacity in European sea-bass. The results show that both additives attenuate fish stress response and improve antioxidant status, indicating their potential to reduce the negative effects of stress in European sea bass.
Article
Agriculture, Dairy & Animal Science
Giovanni S. Molinari, Michal Wojno, Genciana Terova, Macdonald Wick, Hayden Riley, Jeffrey T. Caminiti, Karolina Kwasek
Summary: The use of adult Largemouth Bass muscle hydrolysates as a protein source for larval fish did not improve their growth performance. Further research is needed to determine the optimal protein source and form to reduce reliance on live feed during the larval stage of Largemouth Bass.
Article
Neurosciences
Tiziana Romanazzi, Daniele Zanella, Manan Bhatt, Angela Di Iacovo, Aurelio Galli, Elena Bossi
Summary: Bile acids (BAs) synthesized from cholesterol in the liver play a key role in emulsifying fats for absorption. Recent evidence suggests that BAs can modulate the activity of neuronal receptors and transporters, including the dopamine transporter (DAT), involved in gut-brain signaling. This study investigated the effects of BAs on three transporters and found that obeticholic acid (OCA), a semi-synthetic BA, generated an inward current in DAT, GABA transporter 1 (GAT1), and glycine transporter 1 (GlyT1b), proportional to the current generated by their respective substrates. The findings support a molecular model suggesting the ability of BAs to occlude the transporter, avoiding small depolarizations and enhancing neurotransmitter action.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Review
Physiology
Manan Bhatt, Laure Gauthier-Manuel, Erika Lazzarin, Rocco Zerlotti, Christine Ziegler, Andre Bazzone, Thomas Stockner, Elena Bossi
Summary: γ-Aminobutyric acid (GABA) acts as the primary inhibitory neurotransmitter in the CNS, regulated by GABA transporters (GATs). Although GAT1 has been extensively studied, the roles of other GABA transporters, especially BGT-1, remain unclear. This review aims to compare GAT1 and BGT-1, leveraging the knowledge of GAT1 to shed light on the unanswered questions regarding BGT-1.
FRONTIERS IN PHYSIOLOGY
(2023)
Review
Agriculture, Dairy & Animal Science
Imam Hasan, Simona Rimoldi, Giulio Saroglia, Genciana Terova
Summary: Aquaculture is a rapidly growing industry, but the use of fishmeal as a feed ingredient is not sustainable in the long-term. Insect meals and probiotics have emerged as potential alternatives to fishmeal and have shown positive effects on fish growth and health.
Article
Physiology
Francesca Vacca, Ana S. Gomes, Marco De Gennaro, Ivar Ronnestad, Elena Bossi, Tiziano Verri
Summary: The transport capability of charged dipeptides in teleosts is related to the position of the charged amino acid. In Atlantic salmon, the transport currents of PepT1a and PepT1b are similar, while in zebrafish, PepT1a is highly dependent on the position of Asp and PepT1b has low transport currents.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Antonio Serradell, Daniel Montero, Genciana Terova, Simona Rimoldi, Alex Makol, Felix Acosta, Aline Bajek, Pierrick Haffray, Francois Allal, Silvia Torrecillas
Summary: Husbandry practices in aquaculture may cause stress and oxidative damage to fish tissues. Functional ingredients can reinforce the fish's antioxidant response and stress tolerance. Selective breeding strategies have also shown a correlation between fish growth and stress reactiveness, which is important for species domestication.
Meeting Abstract
Biochemistry & Molecular Biology
Ludovica Iovino, Veronica Giusti, Francesca Pischedda, Elena Giusto, Nicoletta Plotegher, Antonella Marte, Ilaria Battisti, Angela Di Iacovo, Algerta Marku, Giovanni Piccoli, Rina Bandopadhyay, Carla Perego, Tiziana Bonifacino, Giambattista Bonanno, Cristina Roseti, Elena Bossi, Giorgio Arrigono, Luigi Bubacco, Elisa Greggio, Sabine Hilfiker, Laura Civiero
JOURNAL OF NEUROCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Chiara Ceccotti, Ilaria Biasato, Laura Gasco, Christian Caimi, Sara Bellezza Oddon, Simona Rimoldi, Fabio Brambilla, Genciana Terova
Summary: In this study, a feeding trial was conducted with rainbow trout to evaluate the bioavailability and efficacy of five different forms of methionine. The results showed that different forms of methionine had no effects on growth performance but led to changes in the whole-body composition of the fish. Additionally, specific forms of methionine affected hepatic methionine metabolism through different molecular pathways.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
