4.7 Article

Resistance selection and biochemical characterization of spinosad resistance in Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae)

Journal

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
Volume 95, Issue 2, Pages 90-94

Publisher

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pestbp.2009.07.003

Keywords

Helicoverpa armigera; Spinosad; Cross-resistance; Synergism; Carboxyl esterase; Glutathione-S-transferase; p-Nitroanisole O-demethylase

Funding

  1. National Basic Research Program [2006CB102003]
  2. Shandong Natural Science Foundation of China [J2007D43]

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A Helicoverpa armigera population was collected from Shandong province, China. After 15 generations of selection in the laboratory, the H. armigera strain developed more than 20-fold resistance to spinosad. At LD50 level, no significant cross-resistance was found between spinosad and chlorpyrifos, methomyl, avermectin and chlorfenapyr except for fenvalerate with a low cross-resistance of 2.4-fold. However, LD99 values of fenvalerate against the parental and resistant strains were not different significantly. After inhibitors were used, spinosad resistance could be partially suppressed by piperonylbutoxide (PBO) and triphenylphosphate (TPP), but not by diethylmaleate (DEM). Activities of p-nitroanisole O-demethylase (ODM) developed to 8.26-fold compared with the parental strain, but no obvious changes were found in activities of carboxyl esterase (CarE) and glutathione-S-transferase (GST). The results indicated that resistance to spinosad in the cotton bollworm might be associated with an increase in cytochrome P450 monooxygenase. (C) 2009 Elsevier Inc. All rights reserved.

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