Article
Agricultural Engineering
Dhruv Agrawal, Adrian Tsang, Bhupinder Singh Chadha
Summary: Two lignocellulolytic accessory enzymes were cloned and produced successfully in this study. They showed optimal activity under specific conditions and played important roles in the hydrolysis of pre-treated lignocellulosic biomass.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Laura Ramirez-Gonzalez, Elizabeth Undiano, Ivan Flores-Perez, Luis Carrillo-D'Lacoste, Monica Salmeron, Alejandra Verastegui, Gabriela Lara, Antonio Monroy-Noyola
Summary: This study demonstrates the hydrolysis ability of O-hexyl 2,5-dichlorophenyl phosphoramidate (HDCP) compound in reptile sera, which is resistant to EDTA and dependent on Ca2+ and Cu2+ concentrations. Cu2+ activates the hydrolysis of HDCP in freshwater turtle sera, while marine turtles and crocodiles show decreased activity. Ca2+ does not show a significant activating effect on HDCPase activity in any reptile sera. The study also identifies serum albumin as the cuproprotein responsible for this activity.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Agricultural Engineering
Hongling Wang, Xianghui Qi, Song Gao, Yifeng Zhang, Yingfeng An
Summary: The xylanase and feruloyl esterase domains of the bifunctional enzyme Xyn-Fae were identified, and a more efficient bifunctional enzyme XynII-Fae was constructed. The synergistic action of XynII-Fae with cellulase dramatically improved the hydrolysis efficiency of lignocellulose.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Leslie Lam, Marc A. Ilies
Summary: Esterases and lipases can process esters used as drugs and prodrugs, affecting their pharmacokinetics and biodistribution. The activity of these enzymes is highly dependent on the lipophilicity of the substrate, with classic esterases showing decreased activity and lipases generally displaying increased activity as lipophilicity increases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Xiangyu Zhang, Xiaowen Lei, Kehui Ouyang, Wenjing Zhang, Chanjuan Liu, Yanjiao Li, Qinghua Qiu, Yitian Zang, Mingren Qu, Ke Pan, Xianghui Zhao
Summary: Ferulic acid esterases can enzymatically break down hemicellulose to release ferulic acid. This study investigated a ferulic acid esterase from Lentinula edodes (LeFae) and its effects on wheat straw fermentation. LeFae showed optimal pH and temperature at pH 7.0 and 60°C, respectively, exhibited broad adaptability to temperature and pH, and had good thermal stability. LeFae enhanced wheat straw hydrolysis and inhibited microbial fermentation, reducing digestibility and affecting rumen microorganisms.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Lukas R. Dahlin, Alex W. Meyers, Skylar W. Stefani, Ellsbeth G. Webb, Benton Wachter, Venkataramanan Subramanian, Michael T. Guarnieri
Summary: Rising global greenhouse gas emissions and climate change necessitate the development of carbon capture and conversion technologies. A bio-based conversion method, known as the formate bio-economy, has been proposed to use CO2-derived formate as a substrate for microbial systems. While this approach has been explored in some organisms, its utilization in phototrophic organisms has not been reported. In this study, researchers have successfully established formate utilization in a eukaryotic microalga, Picochlorum renovo, using a formate dehydrogenase enzyme. This work has significant implications for applied phototrophic cultivation systems and the bio-economy as a whole.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Zijian Wang, Qingzhu Zhang, Guoqiang Wang, Wenxing Wang, Qiao Wang
Summary: This study systematically investigated the hydrolysis mechanism of methomyl catalyzed by esterase PestE. The results provided atomic-level details of the mechanism and free energy profiles. Serine-initiated nucleophilic attack was identified as the rate-determining step, and the importance of protein-substrate interactions and key active site residues was elucidated.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Organic
Olga Gherbovet, Fernando Ferreira, Apolline Clement, Melanie Ragon, Julien Durand, Sophie Bozonnet, Michael J. O'Donohue, Regis Faure
Summary: This study utilized a chemoenzymatic approach to synthesize different ferulate compounds, shortening synthetic routes and enhancing transesterification yields. A novel feruloylated compound was also successfully synthesized. These findings provide new substrate options for characterizing feruloyl esterases.
BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Xin Zhao, Yuchao Wang, Longsheng Zhan, Mengjie Liu, Jiao Wu, Danni Deng, Jiabi Jiang, Xinran Zheng, Xiang Xiong, Yongpeng Lei
Summary: This study achieved higher selectivity and activity in CO2 reduction using In-doped SnO2 catalyst, addressing the issues of low electrical conductivity and weak CO2 activation.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Minghui Li, Dapeng Zhang, Kaifang Wu, Yuhang Liu, Peng Wang, Yonggang Cao, Jian Yang
Summary: Electroreduction of CO2 to valuable chemicals powered by renewable electricity provides a sustainable approach to reduce environmental issues from CO2 emission. Precisely regulating the strain of atomically dispersed NiN4 active sites enhances CO selectivity and CO2 electroreduction performance.
Article
Chemistry, Multidisciplinary
Minghui Li, Dapeng Zhang, Kaifang Wu, Yuhang Liu, Peng Wang, Yonggang Cao, Jian Yang
Summary: Electroreduction of CO2 to valuable chemicals using renewable electricity can reduce environmental issues caused by CO2 emissions. Precise regulation of the active sites in the CO2 reduction reaction (CO2RR) can improve reaction efficiency and selectivity.
Article
Plant Sciences
Qiyu He, Jianfeng Jin, Pengfei Li, Huihui Zhu, Zhanqi Wang, Wei Fan, Jian Li Yang
Summary: The study identified and characterized the SlFDH gene in tomato and found that it plays a role in aluminum tolerance. The expression of SlFDH was induced by aluminum stress but not by other metals. CRISPR/Cas9-generated mutants lacking the SlFDH gene were more sensitive to aluminum stress and formate. The transcription factor SlSTOP1 was found to positively regulate SlFDH expression by directly binding to its promoter.
Article
Microbiology
Michael Agne, Lena Appel, Carola Seelmann, Matthias Boll
Summary: Syntrophic bacteria play a key role in the conversion of biological matter to methane. They can convert fatty acids or alcohols to H-2, formate, and acetate that serve as substrates for methanogenic archaea. The mechanism by which syntrophic bacteria convert unsaturated fatty acids such as crotonate without a partner has remained unresolved. This study provides evidence that during growth with crotonate, the oxidation of 3-hydroxybutyryl-CoA to acetoacetyl-CoA is coupled to the reduction of crotonyl-CoA via formate cycling. This unique energy metabolism, known as enoyl-CoA respiration, generates a proton motive force via a redox-loop.
Article
Chemistry, Medicinal
Javier Rodriguez-Salarichs, Mario Garcia de Lacoba, Alicia Prieto, Maria Jesus Martinez, Jorge Barriuso
Summary: The study reveals that certain lipases from the Candida rugosa-like family exhibit wide substrate specificity on both triglycerides and sterol esters. By analyzing the full catalytic mechanisms of these enzymes, insights into their mechanistic properties have been gained. A computational protocol was developed to predict substrate specificity, using C. rugosa and O. piceae lipases as model enzymes and different triglycerides and cholesterol esters as model substrates, and the protocol was validated by comparing results with existing literature, offering a valuable tool for virtual screening of substrates for enzymes of the C. rugosa-like family with unknown catalytic properties.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2021)
Article
Biotechnology & Applied Microbiology
Jaehyun Cha, Jinhee Lee, Byoung Wook Jeon, Yong Hwan Kim, Inchan Kwon
Summary: This study proposes a CO2 hydrogenation system that utilizes hydrogenase from Ralstonia eutropha H16 (ReSH) and formate dehydrogenase from Methylobacterium extorquens AM1 (MeFDH1) to efficiently convert carbon dioxide in industrial flue gas into formate via a nicotinamide adenine dinucleotide-dependent cascade reaction. The enzyme system is tolerant to inhibitors such as carbon monoxide and oxygen, and it can utilize diverse sources of hydrogen and carbon dioxide.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)