Article
Plant Sciences
Vanessa Vital Silva, Rafael Mendes, Andreia Suzukawa, Fernando Adegas, Francismar Marcelino-Guimaraes, Rubem Oliveira
Summary: The study confirmed cross-resistance of a weed population in Brazil to ALS inhibitors and identified a mutation in the ALS gene. This is the first study in Brazil to identify a target-site change as a survival mechanism in Erigeron sumatrensis for resistance to ALS-inhibiting herbicides.
Article
Agronomy
Yi Cao, Xinxin Zhou, Zhaofeng Huang
Summary: The study revealed the molecular basis for resistance of Amaranthus retroflexus to ALS-inhibiting herbicides, with Trp-574-Leu and Gly-654-Tyr substitutions in ALS contributing to broad-spectrum resistance. The Gly-654-Tyr substitution, previously unreported, conferred high resistance to multiple classes of ALS-inhibiting herbicides in the R-Tyr654 population. Despite this resistance, effective control of the population was achieved with herbicides targeting other sites of action.
PEST MANAGEMENT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Zhaofeng Huang, Zongzhi Lu, Hongjuan Huang, Wenyu Li, Yi Cao, Shouhui Wei
Summary: Green foxtail is a troublesome weed in maize fields in China, with many populations showing high resistance to the herbicide nicosulfuron. Resistance was found to be associated with two amino acid mutations in the ALS gene and potentially mediated by cytochrome P450s. The resistant populations were also cross-resistant to imazethapyr but sensitive to other herbicides.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nan Wang, Shuang Bai, Feng Bei, Ning Zhao, Sisi Jia, Tao Jin, Jinxin Wang, Hengzhi Wang, Weitang Liu
Summary: This study reports on a population of Myosoton aquaticum L. that exhibits resistance to herbicides in the absence of known acetolactate synthase (ALS) resistance mutations, highlighting the role of non-target-site resistance mechanisms.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jian Li, Yanbang Li, Feng Fang, Dexing Xue, Rumei Li, Xingxiang Gao, Mei Li
Summary: Herbicide-resistant weeds pose a serious threat to world food production, and the rapid and widespread development of resistance limits the application of herbicides. This study identified a highly resistant blackgrass population TW18 in Shandong Province, China, which showed resistance to multiple herbicides due to a F206Y mutation in the ALS gene. Transgenic studies confirmed that this mutation conferred resistance to imazethapyr in Arabidopsis and rice.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Plant Sciences
Ingvild Loubet, Lucie Meyer, Severine Michel, Fanny Pernin, Sebastien Carrere, Benoit Barres, Valerie Le Corre, Christophe Delye
Summary: This study explores the diversity of non-target site resistance (NTSR) mechanisms in common ragweed populations from different regions in France. Transcriptome analysis identifies a wide range of genes associated with NTSR and reveals population-specific evolution in response to herbicide selective pressure. The findings contribute to a better understanding of the complexity and diversity of NTSR mechanisms.
Article
Plant Sciences
Maolong Hu, Hongkun Zhang, Lingna Kong, Juanjuan Ma, Ting Wang, Xinyu Lu, Yue Guo, Jiefu Zhang, Rongzhan Guan, Pu Chu
Summary: The application of herbicides is an effective strategy for weed control, and the development of herbicide-resistant crops can enhance weed management. However, the use of the herbicide tribenuron-methyl (TBM) is limited in rapeseed fields due to rapeseed's sensitivity to TBM. A study was conducted on the TBM-resistant rapeseed mutant M342 and its wild-type plants, revealing that M342 exhibited improved tolerance to TBM and had higher levels of proteins related to non-target-site resistance (NTSR) to herbicides compared to the wild-type plants. Differentially accumulated proteins (DAPs) between the two genotypes were enriched in glutathione metabolism and oxidoreduction coenzyme metabolic process, providing protection against oxidative stress caused by TBM. These findings offer insights into the mechanism of NTSR in plants and contribute to the development of herbicide-resistant crops.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Bochui Zhao, Xian Xu, Binghua Li, Zhizun Qi, Jinan Huang, Ali Hu, Guiqi Wang, Xiaomin Liu
Summary: This study found that a population of D. sanguinalis from Hebei Province, China, displayed 6.9-fold resistance to nicosulfuron. Both ALS gene mutation and increased activity of GSTs enzyme were identified as the resistance mechanisms.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Hossein Ghanizadeh, Christopher E. Buddenhagen, Andrew G. Griffiths, Kerry C. Harrington, Zachary Ngow
Summary: Resistance to iodosulfuron, an herbicide targeting acetolactate synthase (ALS), was identified in a population of Lolium perenne L. from Canterbury, New Zealand. The resistance was due to enhanced herbicide metabolism and a target-site gene mutation. This study provides insights into the resistance mechanisms of ALS-inhibiting herbicides in Lolium perenne and highlights the importance of integrated weed management approaches.
NEW ZEALAND JOURNAL OF AGRICULTURAL RESEARCH
(2022)
Article
Plant Sciences
Candelario Palma-Bautista, Jose G. Vazquez-Garcia, Maria D. Osuna, Blanca Garcia-Garcia, Joel Torra, Joao Portugal, Rafael De Prado
Summary: A study in southern Spain found that Sinapis alba populations have developed resistance to the acetolactate synthase-inhibiting herbicide tribenuron-methyl. The resistant populations showed significantly higher resistance to tribenuron-methyl compared to the susceptible population. A mutation in the ALS gene and P450-mediated metabolism were identified as contributing factors to the resistance. Alternative chemical control methods were effective in controlling both the resistant and susceptible populations.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Environmental Sciences
Ning-Hai Lu, Meng-Yuan Zhang, Kun Jiang, Gui-Lei Hu, Bai-Zhong Zhang
Summary: Descurainia Sophia (L.) is a common weed that causes serious damage in wheat fields worldwide. It is primarily controlled by chemical herbicides, but the unscientific application of herbicides has led to the development of resistance in different populations of D. sophia. This study determined the mutational sites of the ALS gene in four populations and found that point mutations and down-regulation of ALS mRNA expression may play a role in the resistance of D. sophia to ALS inhibitor herbicides. These findings are valuable for managing ALS inhibitor herbicide-resistant D. sophia in wheat fields in China.
FRESENIUS ENVIRONMENTAL BULLETIN
(2022)
Article
Agronomy
Barbara Wrzesinska, Tadeusz Praczyk
Summary: Centaurea cyanus, a weed species commonly found in fields with cereals, sugar beet, and maize, shows resistance mainly against acetolactate synthase (ALS) inhibitors. Genetic variability in ALS sequence has been observed among resistant plants, with four amino acid sequence changes identified in some resistant plants compared to susceptible ones. Further research is needed to confirm the significance of these mutations in herbicide resistance development.
Article
Biology
Rui Zhang, Sha Chen, Xiangbing Meng, Zhuangzhuang Chai, Delin Wang, Yuge Yuan, Kunling Chen, Linjian Jiang, Jiayang Li, Caixia Gao
Summary: Utilizing genome editing, various missense mutations were created in rice to confer tolerance to different herbicides, with a novel triple mutant showing high tolerance to all tested herbicides. Field trials suggested that these mutated varieties have potential practical applications in weed control.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Environmental Sciences
Fan-Bin Kong, Gui-Lei Hu, Kun Jiang, Bai-Zhong Zhang
Summary: Ae. tauschii populations in China have developed resistance to herbicides, particularly to mesosulfuron. A122V mutation in the ALS gene is believed to play an important role in this resistance. Furthermore, the down-regulation of ALS mRNA relative expression quantity is associated with resistance. These findings are valuable for managing herbicide-resistant Ae. tauschii in wheat fields in China.
FRESENIUS ENVIRONMENTAL BULLETIN
(2022)
Article
Biochemistry & Molecular Biology
Caiyue Liu, Tianbao Zhang, Xinsen Yang, Liu Wang, Yan Long, Agula Hasi, Xinwu Pei
Summary: In this study, a mutant with resistance to ALS-inhibiting herbicides was obtained from the elite cultivar Longya10 through EMS mutagenesis. The mutant showed significantly increased resistance to TBM and slight resistance to imazethapyr compared to Longya10. Biochemical analysis revealed a Pro197Ser mutation in the LuALS1 gene, which conferred high resistance to sulfonylurea herbicides in the mutant. Two CAPS markers were developed based on this mutation site for marker assistant selection in breeding. The mutant did not cause yield losses in natural field conditions, making it a valuable germplasm resource for breeding herbicide-resistant flax varieties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Chun Zhang, Qin Yu, Heping Han, Chaojie Yu, Alex Nyporko, Xingshan Tian, Hugh Beckie, Stephen Powles
Summary: This study reveals a naturally occurring mutation in glutamine synthetase that confers resistance to glufosinate in Eleusine indica. The molecular basis of target site glufosinate resistance in E. indica was established, and a mutant EiGS1-1 gene was found to confer glufosinate resistance in rice. Structural modeling showed that the mutation in EiGS1-1 affects important binding residues, suggesting parallel evolution of this resistance mutation.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Agronomy
Yanhui Wang, Heping Han, Jinyi Chen, Qin Yu, Martin Vila-Aiub, Hugh J. Beckie, Stephen B. Powles
Summary: The rare resistance mutation Arg-243-Met has a detrimental effect on fitness when plants are homozygous for this mutation, causing high plant mortality, severely diminished growth, and very poor fecundity. In contrast, plants with the Val-202-Phe mutation exhibit a fitness advantage in growth and reproduction. These contrasting effects on fitness help explain the absence of plants homozygous for the Arg-243-Met mutation and the high frequency of plants carrying the Val-202-Phe mutation in dinitroaniline-resistant populations of Lolium rigidum.
PEST MANAGEMENT SCIENCE
(2022)
Article
Agronomy
Jinyi Chen, Qin Yu, Mechelle Owen, Heping Han, Eric Patterson, Chad Sayer, Stephen Powles
Summary: This study screened 21 putative resistant field survey populations from Western Australia with trifluralin, finding that 90% of them contained individuals surviving trifluralin treatment at 480 g/ha. The study identified known alpha-tubulin resistance mutations in TUA4 and newly identified mutations in TUA3/TUA4, confirming a high incidence of resistance to trifluralin in annual ryegrass populations and highlighting the need for diverse management tactics to address both target-site and non-target-site resistance.
PEST MANAGEMENT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Dongya Wu, Enhui Shen, Bowen Jiang, Yu Feng, Wei Tang, Sangting Lao, Lei Jia, Han-Yang Lin, Lingjuan Xie, Xifang Weng, Chenfeng Dong, Qinghong Qian, Feng Lin, Haiming Xu, Huabing Lu, Luan Cutti, Huajun Chen, Shuiguang Deng, Longbiao Guo, Tse-Seng Chuah, Beng-Kah Song, Laura Scarabel, Jie Qiu, Qian-Hao Zhu, Qin Yu, Michael P. Timko, Hirofumi Yamaguchi, Aldo Merotto, Yingxiong Qiu, Kenneth M. Olsen, Longjiang Fan, Chu-Yu Ye
Summary: This study provides genomic insights into the dual roles of Echinochloa species as weeds and crops, revealing the complex evolution and constrained disease-related gene copy numbers in Echinochloa. The research also uncovers deep population differentiation, herbicide resistance mutations, and limited domestication of barnyard millets. These results offer essential resources for studying plant polyploidization, adaptation, precision weed control, and millet improvements.
NATURE COMMUNICATIONS
(2022)
Article
Engineering, Environmental
Lang Pan, Qiushuang Guo, Junzhi Wang, Li Shi, Xiao Yang, Yaoyu Zhou, Qin Yu, Lianyang Bai
Summary: Long-term and excessive herbicide use has led to the evolution of herbicide resistance in weeds. This study confirmed the resistance of a global weed population, Echinochloa crus-galli, to ALS-inhibiting herbicide penoxsulam and ACCase-inhibiting herbicides cyhalofop-butyl and metamifop. The resistance was found to be due to the higher expression of a cytochrome P450 gene, CYP81A68, and enhanced metabolism via O-demethylation. Epigenetic regulation, specifically differential methylation of CpG islands, was also observed between resistant and susceptible populations. This research highlights the importance of alternative herbicide selection and nonchemical control tactics in reducing environmental impacts.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Agronomy
Julio A. Scursoni, Daniel Tuesca, Federico Balassone, Juan P. Morello, Daniela Medina Herrera, Maria C. Lescano, Nicolas Montero Bulacio, Roberto J. Crespo, Mara B. Depetris, Martin M. Vila-Aiub
Summary: This study evaluates the sensitivity of 50 smooth pigweed accessions to different herbicides. The results show that most accessions are susceptible to 2,4-D and dicamba, while showing high survival rates to glyphosate. Some accessions also show higher survival rates to fomesafen and topramezone, indicating genetic variability in smooth pigweed. Weed management practices should be prioritized to preserve the efficacy of these commonly used herbicides.
Article
Agronomy
Arthur Arrobas Martins Barroso, Thomas Bruno Michelon, Pedro Luis da Costa Aguiar Alves, Heping Han, Qin Yu, Stephen B. Powles, Martin M. Vila-Aiub
Summary: Goosegrass (Eleusine indica) with double Thr-102-Ile + Pro-106-Ser (TIPS) EPSPS mutations shows high resistance to glyphosate, but also incurs a substantial fitness cost. Soybean competition further increases this fitness cost, resulting in 95%, 95%, and 96% reductions in vegetative growth, seed mass, and seed number, respectively. Glyphosate treatment enhances growth in TIPS plants compared to untreated plants. Conversely, moderate glyphosate resistance mutation P106S shows decreased survival rate, vegetative growth, aboveground biomass (34%), seed mass (48%), and number (52%) when treated with glyphosate alone. However, under the combined effects of soybean competition and field-recommended glyphosate dose, both P106S and TIPS plants have significantly limited vegetative growth, aboveground biomass, seed mass, and number (≤99%).
PEST MANAGEMENT SCIENCE
(2022)
Article
Agronomy
Sofia Marques Hill, Martin Vila-Aiub, Manuel Hernandez, Tiago E. Kaspary, Milton A. Garcia
Summary: This study found that herbicide resistance in LOLMU is widespread in agricultural regions of Uruguay, with a significant number of cases showing resistance to multiple herbicides. If not addressed quickly and substantially, the resistance is expected to increase rapidly.
Article
Agronomy
Feng-Yan Zhou, Heping Han, Yun-Jing Han, Alex Nyporko, Qin Yu, Hugh J. J. Beckie, Stephen B. B. Powles
Summary: The expression of AKR genes (LrAKR4C10 and LrAKR1) in glyphosate-resistant populations of Lolium rigidum from Western Australia is induced by glyphosate and 2,4-D, resulting in enhanced glyphosate metabolism and moderate resistance to glyphosate.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agriculture, Multidisciplinary
Huan Lu, Yingze Liu, Mengshuo Li, Heping Han, Fengyan Zhou, Alex Nyporko, Qin Yu, Sheng Qiang, Stephen Powles
Summary: A wild radish population has developed cross-resistance to HPPD-inhibiting herbicides without previous exposure, thanks to enhanced metabolism. The study identified three highly expressed genes in resistant plants, and overexpression of these genes provided resistance against specific herbicides. The findings suggest that overexpression of multiple herbicide-metabolizing genes contributes to herbicide resistance in the wild radish population.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Plant Sciences
Junzhi Wang, Lei Lian, Jiale Qi, Yuhang Fang, Alex Nyporko, Qin Yu, Lianyang Bai, Lang Pan
Summary: Frequent herbicide use leads to the development of weed resistance. A P450 gene (BsCYP81Q32) was identified in Beckmannia syzigachne, a problematic weed, which was found to confer resistance to three herbicides in transgenic rice. The overexpression of the rice ortholog gene OsCYP81Q32 also increased resistance. In contrast, knockout of OsCYP81Q32 using CRISPR/Cas9 made rice more sensitive to one herbicide. Overexpression of BsCYP81Q32 resulted in enhanced metabolism of one herbicide, leading to reduced herbicidal effect. A transcription factor (BsTGAL6) was found to regulate BsCYP81Q32 expression. Inhibition of BsTGAL6 expression changed the plant's response to the herbicide. Sequence polymorphisms in the promoter region of BsTGAL6 might explain differences in expression between resistant and susceptible plants.
Article
Multidisciplinary Sciences
Chun Zhang, Nicholas A. Johnson, Nathan Hall, Xingshan Tian, Qin Yu, Eric L. Patterson
Summary: This study investigates the mechanism of glyphosate resistance in goosegrass, finding that resistance is associated with copy number variation (CNV) of the EPSPS gene, with the variation being driven by sub-telomeric-repeat sequences. This discovery further highlights the importance of subtelomeres in generating genetic variation and provides a unique example of herbicide resistance evolution.
NATURE COMMUNICATIONS
(2023)
