Article
Agronomy
Yi Cao, Xinxin Zhou, Zhaofeng Huang
Summary: The study revealed the molecular basis for resistance of Amaranthus retroflexus to ALS-inhibiting herbicides, with Trp-574-Leu and Gly-654-Tyr substitutions in ALS contributing to broad-spectrum resistance. The Gly-654-Tyr substitution, previously unreported, conferred high resistance to multiple classes of ALS-inhibiting herbicides in the R-Tyr654 population. Despite this resistance, effective control of the population was achieved with herbicides targeting other sites of action.
PEST MANAGEMENT SCIENCE
(2022)
Article
Agronomy
Lei Chen, Gang Gu, Chengxu Wang, Zhufeng Chen, Wei Yan, Man Jin, Gang Xie, Junli Zhou, Xing Wang Deng, Xiaoyan Tang
Summary: Herbicide resistance is valuable for integrated weed management in agriculture, particularly in rice fields where weedy rice poses a challenge. This study obtained three mutant variants of the OsALS gene in rice through ethyl methyl sulfonate mutagenesis, with W-548 M mutant showing high levels of resistance to a broad spectrum of herbicides. The stable inheritance of herbicide resistance in rice lines makes W-548 M mutation a valuable resource for breeding herbicide resistant rice and weed management.
Article
Biology
Rui Zhang, Sha Chen, Xiangbing Meng, Zhuangzhuang Chai, Delin Wang, Yuge Yuan, Kunling Chen, Linjian Jiang, Jiayang Li, Caixia Gao
Summary: Utilizing genome editing, various missense mutations were created in rice to confer tolerance to different herbicides, with a novel triple mutant showing high tolerance to all tested herbicides. Field trials suggested that these mutated varieties have potential practical applications in weed control.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Plant Sciences
Candelario Palma-Bautista, Jose G. Vazquez-Garcia, Maria D. Osuna, Blanca Garcia-Garcia, Joel Torra, Joao Portugal, Rafael De Prado
Summary: A study in southern Spain found that Sinapis alba populations have developed resistance to the acetolactate synthase-inhibiting herbicide tribenuron-methyl. The resistant populations showed significantly higher resistance to tribenuron-methyl compared to the susceptible population. A mutation in the ALS gene and P450-mediated metabolism were identified as contributing factors to the resistance. Alternative chemical control methods were effective in controlling both the resistant and susceptible populations.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Barbara Wrzesinska, Tadeusz Praczyk
Summary: Centaurea cyanus, a weed species commonly found in fields with cereals, sugar beet, and maize, shows resistance mainly against acetolactate synthase (ALS) inhibitors. Genetic variability in ALS sequence has been observed among resistant plants, with four amino acid sequence changes identified in some resistant plants compared to susceptible ones. Further research is needed to confirm the significance of these mutations in herbicide resistance development.
Article
Plant Sciences
Maolong Hu, Hongkun Zhang, Lingna Kong, Juanjuan Ma, Ting Wang, Xinyu Lu, Yue Guo, Jiefu Zhang, Rongzhan Guan, Pu Chu
Summary: The application of herbicides is an effective strategy for weed control, and the development of herbicide-resistant crops can enhance weed management. However, the use of the herbicide tribenuron-methyl (TBM) is limited in rapeseed fields due to rapeseed's sensitivity to TBM. A study was conducted on the TBM-resistant rapeseed mutant M342 and its wild-type plants, revealing that M342 exhibited improved tolerance to TBM and had higher levels of proteins related to non-target-site resistance (NTSR) to herbicides compared to the wild-type plants. Differentially accumulated proteins (DAPs) between the two genotypes were enriched in glutathione metabolism and oxidoreduction coenzyme metabolic process, providing protection against oxidative stress caused by TBM. These findings offer insights into the mechanism of NTSR in plants and contribute to the development of herbicide-resistant crops.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Plant Sciences
Sarah Shah, Thierry Lonhienne, Cody-Ellen Murray, Yibi Chen, Katherine E. Dougan, Yu Shang Low, Craig M. Williams, Gerhard Schenk, Gimme H. Walter, Luke W. Guddat, Cheong Xin Chan
Summary: This study identified 12 common herbicide-target genes and their proteins from seven species of Weeds of National Significance in Australia using high-throughput genome sequencing. The study found high sequence conservation at the herbicide-target sites among the diverse weed species. Mutations observed in the proteins of fireweed and parthenium suggest resistance of these weeds to certain herbicides. These findings provide important insights for herbicide discovery and development.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Caiyue Liu, Tianbao Zhang, Xinsen Yang, Liu Wang, Yan Long, Agula Hasi, Xinwu Pei
Summary: In this study, a mutant with resistance to ALS-inhibiting herbicides was obtained from the elite cultivar Longya10 through EMS mutagenesis. The mutant showed significantly increased resistance to TBM and slight resistance to imazethapyr compared to Longya10. Biochemical analysis revealed a Pro197Ser mutation in the LuALS1 gene, which conferred high resistance to sulfonylurea herbicides in the mutant. Two CAPS markers were developed based on this mutation site for marker assistant selection in breeding. The mutant did not cause yield losses in natural field conditions, making it a valuable germplasm resource for breeding herbicide-resistant flax varieties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agronomy
Nicolas Loste, Graciela M. Nestares, Silvina A. Felitti, Ana C. Ochogavia
Summary: Chemically induced male sterility (CIMS) is a valuable tool for hybrid breeding in crops, and the gametocidal effect of the herbicide imazapyr on sunflowers has been investigated at the molecular level. The comparative transcriptomic analysis revealed genes related to xenobiotic metabolism and stress as potentially involved in the male sterility induction process. These findings offer new insights into the molecular mechanisms underlying male sterility induction in sunflowers.
Article
Food Science & Technology
So Hee Yang, Euyeon Kim, Hyosun Park, Yeonjong Koo
Summary: Herbicide resistance is an important trait in improving farming methods and crop productivity. This study demonstrates the usefulness of using CRISPR-Cas9 system to edit herbicide binding protein genes in tomatoes and confirms the feasibility of targeting site editing through sgRNA efficiency testing. The success of breeding herbicide-resistant transgenic tomatoes highlights the value of CRISPR-Cas9 as a tool for crop improvement.
APPLIED BIOLOGICAL CHEMISTRY
(2022)
Article
Agriculture, Multidisciplinary
Juliusz Pernak, Michal Niemczak, Tomasz Rzemieniecki, Katarzyna Marcinkowska, Tadeusz Praczyk
Summary: This study synthesized and characterized dicationic herbicidal ionic liquids containing different herbicidal anions with various modes of action. The HILs showed better performance in weed control compared to traditional herbicides, with the alkyl chain length in the cation influencing efficacy.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agriculture, Multidisciplinary
Hossein Ghanizadeh, Christopher E. Buddenhagen, Andrew G. Griffiths, Kerry C. Harrington, Zachary Ngow
Summary: Resistance to iodosulfuron, an herbicide targeting acetolactate synthase (ALS), was identified in a population of Lolium perenne L. from Canterbury, New Zealand. The resistance was due to enhanced herbicide metabolism and a target-site gene mutation. This study provides insights into the resistance mechanisms of ALS-inhibiting herbicides in Lolium perenne and highlights the importance of integrated weed management approaches.
NEW ZEALAND JOURNAL OF AGRICULTURAL RESEARCH
(2022)
Review
Chemistry, Multidisciplinary
Jean-Paul Bourdineaud
Summary: In France, the implementation of mutant herbicide-tolerant crops and their related herbicides has caused a strong societal reaction. The toxicity of these herbicides to animals, including humans, and the environmental risks they pose are being questioned. Studies have shown that these herbicides impact molecular targets other than their intended enzyme, and they have been associated with increased risk of colon and bladder cancers, as well as miscarriages. The persistence of these herbicides in agricultural soils and their presence in drinking water also raises concerns.
DRUG AND CHEMICAL TOXICOLOGY
(2022)
Article
Plant Sciences
Ryan Widianto, Denny Kurniadie, Dedi Widayat, Uum Umiyati, Ceppy Nasahi, Santika Sari, Abdul Shukor Juraimi, Hisashi Kato-Noguchi
Summary: Monochoria vaginalis is an aquatic herbaceous plant that is often found as a noxious weed in paddy fields. This research evaluated the resistance levels of M. vaginalis to different groups of herbicides and investigated the mutations in the ALS gene of the resistant biotype. The study found that the resistant biotype had varying levels of resistance to different herbicides and had specific amino acid substitutions in the ALS protein. Further research is needed to understand the molecular basis of resistance mechanisms and to develop control strategies.
Article
Environmental Sciences
Ning-Hai Lu, Meng-Yuan Zhang, Kun Jiang, Gui-Lei Hu, Bai-Zhong Zhang
Summary: Descurainia Sophia (L.) is a common weed that causes serious damage in wheat fields worldwide. It is primarily controlled by chemical herbicides, but the unscientific application of herbicides has led to the development of resistance in different populations of D. sophia. This study determined the mutational sites of the ALS gene in four populations and found that point mutations and down-regulation of ALS mRNA expression may play a role in the resistance of D. sophia to ALS inhibitor herbicides. These findings are valuable for managing ALS inhibitor herbicide-resistant D. sophia in wheat fields in China.
FRESENIUS ENVIRONMENTAL BULLETIN
(2022)
Article
Agronomy
Andrea Milani, Ulrich Lutz, Giulio Galla, Laura Scarabel, Detlef Weigel, Maurizio Sattin
Summary: This study investigated the population structure and ALS haplotypes of Italian Amaranthus tuberculatus populations resistant to herbicides. The research revealed that resistance to ALS inhibitors arose independently at least three times, with one population already resistant when introduced in Italy. A single haplotype carrying the Trp574Leu mutation was shared among six populations, likely due to broad seed dispersal. Additionally, one population likely evolved resistance to non-target site ALS inhibitors.
PEST MANAGEMENT SCIENCE
(2021)
Article
Plant Sciences
Laura Ravazzolo, Stephanie Boutet-Mercey, Francois Perreau, Cristian Forestan, Serena Varotto, Benedetto Ruperti, Silvia Quaggiotti
Summary: Nitrate regulates root development in maize through the coordinated action of strigolactones (SLs) and auxin. The study found that nitrogen deficiency induces the biosynthesis of zealactone and carlactonoic acid in roots to a greater extent compared to phosphorus deficiency. The research also identified novel components involved in the molecular regulation of maize root response to nitrate.
PLANT AND CELL PHYSIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Gaetano Giudice, Loredana Moffa, Serena Varotto, Maria Francesca Cardone, Carlo Bergamini, Gabriella De Lorenzis, Riccardo Velasco, Luca Nerva, Walter Chitarra
Summary: This review focuses on challenging biotechnological approaches for breeding crops tolerant to biotic and abiotic stresses, as well as new techniques based on RNA interference and epigenome modifications to improve plant resilience. Recent advances in these applications are mainly reported for non-model plants and woody crops, and their combination with traditional breeding is discussed as a potential contribution to modern agriculture and climate change mitigation.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Multidisciplinary Sciences
Dongya Wu, Enhui Shen, Bowen Jiang, Yu Feng, Wei Tang, Sangting Lao, Lei Jia, Han-Yang Lin, Lingjuan Xie, Xifang Weng, Chenfeng Dong, Qinghong Qian, Feng Lin, Haiming Xu, Huabing Lu, Luan Cutti, Huajun Chen, Shuiguang Deng, Longbiao Guo, Tse-Seng Chuah, Beng-Kah Song, Laura Scarabel, Jie Qiu, Qian-Hao Zhu, Qin Yu, Michael P. Timko, Hirofumi Yamaguchi, Aldo Merotto, Yingxiong Qiu, Kenneth M. Olsen, Longjiang Fan, Chu-Yu Ye
Summary: This study provides genomic insights into the dual roles of Echinochloa species as weeds and crops, revealing the complex evolution and constrained disease-related gene copy numbers in Echinochloa. The research also uncovers deep population differentiation, herbicide resistance mutations, and limited domestication of barnyard millets. These results offer essential resources for studying plant polyploidization, adaptation, precision weed control, and millet improvements.
NATURE COMMUNICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
Monica Gandia, Elena Moreno-Gimenez, Moises Giner-Llorca, Sandra Garrigues, Carolina Ropero-Perez, Antonella Locascio, Pedro Martinez-Culebras, Jose F. Marcos, Paloma Manzanares
Summary: A new fungal biofactory system was developed to produce antifungal proteins (AFPs). Comparisons of different regulatory elements and fungal species revealed their effects on AFP production.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Agronomy
Maria Gerakari, Nikolina Cheimona, Eleni Tani, Ilias Travlos, Demosthenis Chachalis, Donato Loddo, Solvejg Kopp Mathiassen, Thomas K. Gitsopoulos, Laura Scarabel, Silvia Panozzo, Michael Kristensen, Per Kudsk, Maurizio Sattin
Summary: This study investigated the mechanisms responsible for conferring glyphosate resistance in some Lolium spp. populations. The results demonstrated that glyphosate resistance is a multifactor phenomenon. The relative transcript levels of ABC-transporter genes can be used as a useful tool to quickly identify Lolium spp. populations resistant to glyphosate.
Review
Biochemistry & Molecular Biology
Gabriella Consonni, Giulia Castorina, Serena Varotto
Summary: This paper introduces the research work carried out by the Italian scientific community on the genetic control of maize seed development and seed-related pathways, and highlights their contributions to the characterization of maize mutants and the improvement of maize genomics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Andrea Milani, Silvia Panozzo, Samuele Pinton, Renato Antonio Danielis, Maurizio Sattin, Laura Scarabel
Summary: Poor control of Amaranthus spp. with herbicides inhibiting acetolactate synthase (ALS) has been observed in soybean fields in north-eastern Italy. Extensive sampling confirmed the presence of ALS-resistant populations of Amaranthus hybridus and identified mutant ALS alleles responsible for resistance. Pre-emergence herbicides metribuzin, clomazone, and metobromuron can be used as alternative management strategies to limit resistance spread.
Article
Biochemical Research Methods
Silvia Panozzo, Silvia Farinati, Maurizio Sattin, Laura Scarabel
Summary: In this study, the AS-LAMP technique was used to detect herbicide resistance in Lolium spp. The method showed good results in detecting ACCase gene, but had limitations when applied to ALS gene.
Article
Agronomy
Alfredo Manicardi, Andrea Milani, Laura Scarabel, German Mora, Jordi Recasens, Josep Maria Llenes, Jose Maria Montull, Joel Torra
Summary: The invasive weed Amaranthus palmeri is spreading in Spain, particularly in Catalonia. It has developed resistance to acetolactate synthase-inhibiting herbicides and is now suspected to have glyphosate resistance as well. The main resistance mechanism appears to be the overexpression of the enzyme EPSPS due to copy number variation, specifically within the EPSPS cassette.
Article
Microbiology
Carolina Ropero-Perez, Begona Bolos, Moises Giner-Llorca, Antonella Locascio, Sandra Garrigues, Monica Gandia, Paloma Manzanares, Jose F. Marcos
Summary: Fungal infections pose a threat to human health and food security. Antifungal proteins (AFPs) from fungi have the potential to control fungal pathogens, but their biological role and mode of action need further understanding for future application.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biotechnology & Applied Microbiology
Moises Giner-Llorca, Antonella Locascio, Javier Alonso del Real, Jose F. Marcos, Paloma Manzanares
Summary: This study provides insights into the mode of action of PeAfpA by using a model laboratory strain of S. cerevisiae. The results highlight the role of the yeast cell wall in the interaction with PeAfpA, which can be internalized through both energy-dependent and independent mechanisms. Additionally, the study reveals the involvement of the CW integrity pathway and the cAMP-PKA signaling in the PeAfpA killing mechanism.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Agronomy
Alfredo Manicardi, Laura Scarabel, Josep Maria Llenes, Jose Maria Montull, Maria Dolores Osuna, Joel Torra Farre, Andrea Milani
Summary: Armadillo palmeri populations in Spain and Italy have shown resistance to acetolactate synthase (ALS) inhibitors. Mutant ALS alleles, especially 574-Leu, were found to be the main cause of resistance among these populations. Haplotype analyses revealed different evolutionary histories and independent introduction events in both countries.
PEST MANAGEMENT SCIENCE
(2023)
Review
Agronomy
Maor Matzrafi, Laura Scarabel, Andrea Milani, Duilio Iamonico, Joel Torra, Jordi Recasens, Jose Maria Montull, Josep Maria Llenes, Ioannis Gazoulis, Alexandros Tataridas, Baruch Rubin, Gabriel Pardo, Alicia Cirujeda, Ana Isabel Mari, Husrev Mennan, Panagiotis Kanatas, Mehmet Nedim Dogan, Roland Beffa, Ilias Travlos
Summary: Amaranthus palmeri, a fast-growing and high-seed-producing plant, is spreading in Europe and the Mediterranean region. This review highlights the botanical characteristics of A. palmeri and distinguishes it from closely related species. The occurrence of A. palmeri in Europe and the Mediterranean region is summarized, and its future invasion and spread are assessed. Early detection and control measures are reviewed, emphasizing integrated weed management to mitigate the impact of A. palmeri invasion on European agriculture.
Review
Plant Sciences
Elisa Fasani, Gianluigi Giannelli, Serena Varotto, Giovanna Visioli, Diana Bellin, Antonella Furini, Giovanni Dalcorso
Summary: This review summarizes the epigenetic response of plants to soil contamination by heavy metals and metalloids, as well as the transgenerational transfer of this epigenetic status, with consideration of the impact of the microbial community inhabiting the rhizosphere.