Journal
PEPTIDES
Volume 61, Issue -, Pages 75-82Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2014.09.006
Keywords
Anorexia; Body mass index; Glucagon-like peptide 1; Hormone; Pancreatic polypeptide; Plasma
Funding
- German Research Foundation [STE 1765/3-1]
- Charite University Funding [UFF 89-441-176]
- Sonnenfeld Foundation Berlin
Ask authors/readers for more resources
Dipeptidyl peptidase IV (DPPIV) is a protease with broad distribution involved in various homeostatic processes such as immune defense, psychoneuroendocrine functions and nutrition. While DPPIV protein levels were investigated in patients with hyporectic disorders, less is known under conditions of obesity. Therefore, we investigated DPPIV across a broad range of body mass index (BMI). Blood samples from hospitalized patients with normal weight (BMI 18.5-25 kg/m(2)), anorexia nervosa (BMI < 17.5 kg/m(2)) and obesity (BMI 30-40, 40-50 and >50 kg/m(2), n = 15/group) were tested cross-sectionally and DPPIV concentration and total enzyme activity and the DPPIV targets, pancreatic polypeptide (PP) and glucagon-like peptide (GLP-1) were measured. DPPIV protein expression was detected in human plasma indicated by a strong band at the expected size of 110 kDa and another major band at 50 kDa, likely representing a fragment comprised of two heavy chains. Obese patients had higher DPPIV protein levels compared to normal weight and anorexics (+50%, p < 0.05) resulting in a positive correlation with BMI (r = 0.34, p = 0.004). DPPIV serum activity was similar in all groups (p > 0.05), while the concentration/activity ratio was higher in obese patients (p < 0.05). Plasma PP levels were highest in anorexic patients (similar to 2-fold increase compared to other groups, p < 0.05), whereas GLP-1 did not differ among groups (p < 0.05). Taken together, circulating DPPIV protein levels depend on body weight with increased levels in obese resulting in an increased concentration/activity ratio. Since DPPIV deactivates food intake-inhibitory hormones like PP, an increased DPPIV concentration/activity ratio might contribute to reduced food intake-inhibitory signaling under conditions of obesity. (C) 2014 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available