Journal
PEPTIDES
Volume 48, Issue -, Pages 137-146Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2013.08.010
Keywords
Arcuate nucleus of hypothalamus; Diabetes mellitus; Ghrelin; Gastric distention sensitive neurons; Gastric motility; Rats
Funding
- National Natural Science Foundation of China [30470642, 30670780, 31071014, 81100260, 81270460]
- Shandong Province Tackle Key Problems in Science and Technology Program [2008GG10002006]
- Qingdao Municipal Science and Technology Commission [13-1-4-170-jch, 11-2-3-3-(2)-nsh]
- Shandong Province Health Department [2007HZ026]
Ask authors/readers for more resources
This study was performed to observe the effects of ghrelin on the activity of gastric distention (GD) sensitive neurons in the arcuate nucleus of hypothalamus (Arc) and on gastric motility in vivo in streptozocin (STZ) induced diabetes mellitus (DM) rats. Electrophysiological results showed that ghrelin could excite GD-excitatory (GD-E) neurons and inhibit GD-inhibitory (GD-I) neurons in the Arc. However, fewer GD-E neurons were excited by ghrelin and the excitatory effect of ghrelin on GD-E neurons was much weaker in DM rats. Gastric motility research in vivo showed that microinjection of ghrelin into the Arc could significantly promote gastric motility and it showed a dose-dependent manner. The effect of ghrelin promoting gastric motility in DM rats was weaker than that in normal rats. The effects induced by ghrelin could be blocked by growth hormone secretagogue receptor (GHSR) antagonist [D-Lys-3]-GHRP-6 or BIM28163. RIA and real-time PCR data showed that the levels of ghrelin in the plasma, stomach and ghrelin mRNA in the Arc increased at first but decreased later and the expression of GHSR-1a mRNA in the Arc maintained a low level in DM rats. The present findings indicate that ghrelin could regulate the activity of GD sensitive neurons and gastric motility via ghrelin receptors in the Arc. The reduced effects of promoting gastric motility induced by ghrelin could be connected with the decreased expression of ghrelin receptors in the Arc in diabetes. Our data provide new experimental evidence for the role of ghrelin in gastric motility disorder in diabetes. (C) 2013 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available