Article
Plant Sciences
Yue Liu, Yang Li, Huixin Guo, Bingsheng Lv, Jing Feng, Huihui Wang, Zhonghua Zhang, Sen Chai
Summary: The mechanism by which CPPU induces fruit set in melon is unclear. However, histological and morphological observations revealed that CPPU-induced fruits have higher cell density but smaller cell size compared to normal pollinated fruits. CPPU promotes the accumulation of gibberellin and auxin, and decreases the level of abscisic acid during fruit set. Transcriptome analysis further revealed that the key synthase encoding gibberellin 20-oxidase 1 (CmGA20ox1) is specifically upregulated in the GA-related pathway induced by CPPU.
HORTICULTURE RESEARCH
(2023)
Article
Developmental Biology
Aushaq B. Malla, Shannon R. Rainsford, Zachary D. Smith, Bluma J. Lesch
Summary: Unique chromatin remodeling factors regulate nuclear morphology changes during sperm differentiation. Histone-to-protamine exchange is a critical step in this process, and the histone methyltransferase DOT1L plays an essential role in this transition. Loss of DOT1L leads to abnormal sperm head formation and male sterility, due to impaired histone replacement and protamine recruitment. DOT1L also affects transcriptional regulation and gene expression during sperm differentiation.
Article
Oncology
Chiara Bellio, Marta Emperador, Pol Castellano, Albert Gris-Oliver, Francesc Canals, Alex Sanchez-Pla, Esther Zamora, Joaquin Arribas, Cristina Saura, Violeta Serra, Josep Tabernero, Bruce A. Littlefield, Josep Villanueva
Summary: Drug tolerant persister (DTP) cells enter a reversible slow-cycling state after treatment, with GDF15 identified as a key protein over-secreted and essential for their survival. Combining eribulin with an anti-GDF15 antibody may be effective in targeting and killing breast cancer DTP cells.
Article
Cell Biology
Pan Wang, Sheng Gong, Jinyu Pan, Junwei Wang, Dewei Zou, Shuanglong Xiong, Lu Zhao, Qian Yan, Yangming Deng, Nan Wu, Bin Liao
Summary: Combining hyperbaric oxygen and chemotherapy enhances chemotherapy sensitivity in GBM cells by decreasing the expression of HIF1 alpha, HIF2 alpha, and Sox2, thus promoting cell proliferation and chemosensitization.
CELL DEATH DISCOVERY
(2021)
Article
Endocrinology & Metabolism
Caitlin E. Stallings, Jyoti Kapali, Brian W. Evans, Stacey R. McGee, Buffy S. Ellsworth
Summary: Understanding the molecular mechanisms underlying pituitary organogenesis and function is crucial for improving therapeutics and diagnosing hypopituitarism. This study reveals FOXO transcription factors as important regulators of pituitary gland function, affecting somatotrope function and the expression of somatotrope differentiation factors.
Article
Biochemistry & Molecular Biology
Sabrina D'Agosto, Francesco Pezzini, Lisa Veghini, Pietro Delfino, Claudia Fiorini, Gael D. Temgue Tane, Anais Del Curatolo, Caterina Vicentini, Giorgia Ferrari, Davide Pasini, Silvia Andreani, Francesca Lupo, Elena Fiorini, Giulia Lorenzon, Rita T. Lawlor, Borislav Rusev, Antonia Malinova, Claudio Luchini, Michele Milella, Elisabetta Sereni, Antonio Pea, Claudio Bassi, Peter Bailey, Aldo Scarpa, Emilio Bria, Vincenzo Corbo
Summary: Transcriptomic analysis has shown that FGFR1 and FGFR4 play distinct roles in determining the aggressive phenotype of pancreatic ductal adenocarcinoma (PDAC). FGFR4 expression is elevated in the classical PDAC subtype and associated with better outcomes, while FGFR1 expression is associated with the EMT phenotype. Downregulation of FGFR4 leads to increased proliferation and phenotype switch in PDAC cells.
Article
Biochemistry & Molecular Biology
David Milewski, Samriddhi Shukla, Berkley E. Gryder, Arun Pradhan, Johnny Donovan, Parvathi Sudha, Sushmitha Vallabh, Athena Pyros, Yan Xu, Artem Barski, Sara Szabo, Brian Turpin, Joseph G. Pressey, Douglas P. Millay, Javed Khan, Vladimir V. Kalinichenko, Tanya Kalin
Summary: The FOXF1 transcription factor plays a crucial role in FP-RMS tumorigenesis by regulating gene expression associated with FP-RMS gene signature. By cooperating with PAX3-FOXO1 and E-box transcription factors, FOXF1 functions downstream of PAX3-FOXO1 to promote the development of FP-RMS.
Article
Biotechnology & Applied Microbiology
Xiujuan Zhou, Bin Liu, Yanhong Liu, Chunlei Shi, Pina M. Fratamico, Lida Zhang, Dapeng Wang, Jianhua Zhang, Yan Cui, Ping Xu, Xianming Shi
Summary: The deletion of genes SC0368 and SC0595 in Salmonella Choleraesuis led to defects in motility and increased invasiveness in Caco-2 cells. Additionally, SC0368 deletion resulted in up-regulation of genes encoding cytochromes, enhancing bacterial proliferation. These findings suggest that these two genes play important roles in flagella biosynthesis, LPS synthesis, bacterial growth, and virulence within Salmonella C1 serogroup.
Article
Genetics & Heredity
Valeria Scagliotti, Ruben Esse, Thea L. Willis, Mark Howard, Isabella Carrus, Emily Lodge, Cynthia L. Andoniadou, Marika Charalambous
Summary: Imprinted genes play important roles in endocrine processes, but their contributions to the development and function of the hypothalamo-pituitary axis are still not well defined. Technical difficulties in obtaining useful transcriptomic data from the pituitary gland have limited research, but new research using high-sensitivity RNA sequencing and single-cell RNA sequencing has provided insights into the imprinted transcriptome of the pituitary gland.
Article
Microbiology
Georges Potemberg, Aurore Demars, Emeline Barbieux, Angeline Reboul, Francois-Xavier Stubbe, Malissia Galia, Maxime Lagneaux, Audrey Comein, Olivier Denis, David Perez-Morga, Jean-Marie Vanderwinden, Xavier De Bolle, Eric Muraille
Summary: By analyzing the Brucella genome and lung infection, it was found that the immune status determines the genes required for optimal growth and survival of Brucella in vivo.
Article
Biochemistry & Molecular Biology
Nuria Saiz, Lisbeth Herrera-Castillo, Esther Isorna, Maria Jesus Delgado, Marta Conde-Sieira, Jose Luis Soengas, Nuria de Pedro
Summary: This study demonstrates, for the first time in fish, the effects of REV-ERB alpha activation via SR9009, promoting a negative energy balance by reducing energetic inputs and regulating lipid and glucose metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Shunpan Shu, Mingsheng Jiang, Xue Deng, Wenkai Yue, Xu Cao, Kai Zhang, Zeqing Wang, Hao He, Jihong Cui, Qiangqiang Wang, Kun Qu, Yanshan Fang
Summary: In this study, the downregulation of the Drosophila gene CG32529/GLAD in adult flies led to shortened lifespan and age-dependent brain degeneration. The regulation mainly affected the surface glia and cortex glia, disrupting the blood-brain barrier and glial meshwork. The upregulation of immune-related genes was found to be the most significant transcriptional change in response to RNAi-GLAD.
Article
Multidisciplinary Sciences
Xiaowei Sun, Jie Shen, Norbert Perrimon, Xue Kong, Dan Wang
Summary: This study demonstrates the role of the Drosophila endoribonuclease CG2145 (Arlr) in regulating lipid metabolism. Arlr affects lipid homeostasis by degrading the mRNAs of lipolysis genes and this phenotype can be rescued by high-fat or high-glucose diet.
NATURE COMMUNICATIONS
(2023)
Article
Cardiac & Cardiovascular Systems
Hiroyuki Kawagishi, Tsutomu Nakada, Takuro Numaga-Tomita, Maite Larranaga, Ang Guo, Long-Sheng Song, Mitsuhiko Yamada
Summary: Although the regulatory mechanisms and physiological importance of cardiomyocyte proliferation in the early postnatal period have not been clarified, this study found that pharmacological and genetic depletion of gp130 in preweaning mice resulted in significant impairment of cardiomyocyte proliferation, thinning of the myocardium, and systolic dysfunction of the left ventricle by perturbing JAK/STAT3 signaling. Therefore, the IL-6/gp130/JAK/STAT3 axis is crucial for the postnatal functional development of the left ventricle.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Seong Hyeon Hong, Gwidong Han, Seung Jae Lee, Julie Cocquet, Chunghee Cho
Summary: The study reveals that the testicular germ cell-specific lncRNA NR_038002 interacts with HSF2 to regulate the expression of MSYq genes, affecting male mouse fertility and offspring sex ratio. This discovery provides new insights into the molecular mechanism of testis-specific activity.
Article
Genetics & Heredity
Julian C. Lui, Youn Hee Jee, Audrey Lee, Shanna Yue, Jacob Wagner, Deirdre E. Donnelly, Karen S. Vogt, Jeffrey Baron
Article
Biotechnology & Applied Microbiology
Julian C. Lui, Max Colbert, Crystal Sao Fong Cheung, Michal Ad, Audrey Lee, Zhongyu Zhu, Kevin M. Barnes, Dimiter S. Dimitrov, Jeffrey Baron
Article
Endocrinology & Metabolism
Julian C. Lui, Shanna Yue, Audrey Lee, Bijal Kikani, Adrian Temnycky, Kevin M. Barnes, Jeffrey Baron
Article
Genetics & Heredity
Youn Hee Jee, Sehoon Won, Julian C. Lui, Melissa Jennings, Philip Whalen, Yue Shanna, Adrian G. Temnycky, Kevin M. Barnes, Tim Cheetham, Matthew G. Boden, Sally Radovick, Richard Quinton, Ellen W. Leschek, Greti Aguilera, Jack A. Yanovski, Stephanie B. Seminara, William F. Crowley, Angela Delaney, Katherine W. Roche, Jeffrey Baron
GENETICS IN MEDICINE
(2020)
Review
Endocrinology & Metabolism
Julian C. Lui
JOURNAL OF ENDOCRINOLOGY
(2020)
Review
Physiology
Elaine Zhou, Julian Lui
Summary: This review primarily discusses the mechanisms regulating bone length and skeletal proportions.
EXPERIMENTAL PHYSIOLOGY
(2021)
Article
Genetics & Heredity
Youn Hee Jee, Mariam Gangat, Olga Yeliosof, Adrian G. Temnycky, Selena Vanapruks, Philip Whalen, Evgenia Gourgari, Cortney Bleach, Christine H. Yu, Ian Marshall, Jack A. Yanovski, Kathleen Link, Svetlana Ten, Jeffrey Baron, Sally Radovick
Summary: The study found that sporadic congenital hypopituitarism has a significant genetic component, but may less frequently be monogenic with full penetrance, suggesting a more complex etiology.
FRONTIERS IN GENETICS
(2021)
Article
Endocrinology & Metabolism
Birgit Weiss, Birgit Eberle, Ralph Roeth, Christiaan de Bruin, Julian C. Lui, Nagarajan Paramasivam, Katrin Hinderhofer, Hermine A. van Duyvenvoorde, Jeffrey Baron, Jan M. Wit, Gudrun A. Rappold
Summary: Human growth is a complex trait influenced by various gene defects. This study identified genes associated with tall stature and revealed close functional connections between these genes in the context of linear growth.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Multidisciplinary Sciences
Julian C. Lui, Adalbert Raimann, Hironori Hojo, Lijin Dong, Paul Roschger, Bijal Kikani, Uwe Wintergerst, Nadja Fratzl-Zelman, Youn Hee Jee, Gabriele Haeusler, Jeffrey Baron
Summary: SP7/Osterix is a critical transcription factor for osteoblast maturation and bone formation. Mutations in SP7 can cause osteogenesis imperfecta type XII, but neomorphic mutations of SP7 have not been reported in humans. In this study, a de novo dominant neomorphic missense variant (c.926 C > G:p.S309W) in SP7 was found in a patient with craniosynostosis, cranial hyperostosis, and long bone fragility. Mice with the corresponding variant also showed a complex skeletal phenotype. The mutation altered the binding specificity of SP7 and resulted in aberrant gene expression.
NATURE COMMUNICATIONS
(2022)
Article
Medicine, Research & Experimental
Julian C. Lui, Jacob Wagner, Elaine Zhou, Lijin Dong, Kevin M. Barnes, Youn Hee Jee, Jeffrey Baron
Summary: A new type of overgrowth disorder caused by a genetic variant in the SPIN4 gene, an epigenetic reader, has been identified. The variant affects cell proliferation, canonical WNT signaling, and leads to overgrowth both in humans and mice. This study expands our understanding of epigenetic regulation of human growth.