Article
Medicine, Research & Experimental
Defu Chen, David W. Nauen, Hyeon-Cheol Park, Dawei Li, Wu Yuan, Ang Li, Honghua Guan, Carmen Kut, Kaisorn L. Chaichana, Chetan Bettegowda, Alfredo Quinones-Hinojosa, Xingde Li
Summary: This study systematically investigated the feasibility of using multiphoton microscopy for rapid histopathological assessment of label-and processing-free surgical specimens, focusing on infiltrating glioma. The results showed that multiphoton microscopy robustly captures diagnostic features of glioma and deep learning can assist with image recognition.
Review
Physics, Multidisciplinary
Huiling Zhan, Caihong Sun, Mingyu Xu, Tianyi Luo, Guangxing Wang, Gangqin Xi, Zhiyi Liu, Shuangmu Zhuo
Summary: Novel fast microscopy imaging technologies such as SRS, PAM, MPM, and OCM have broad application prospects in tumor resection surgeries, offering more advantages compared to traditional methods. The proposed multi-mode hybrid detection technology combined with deep learning-based artificial intelligence can serve as the basis for future intraoperative diagnosis.
FRONTIERS IN PHYSICS
(2022)
Article
Medicine, Research & Experimental
Despoina Kantere, Noora Neittaanmaki, Kristina Maltese, Ann-Marie Wennberg Larko, Petra Tunback
Summary: This study investigated the potential of reflectance confocal microscopy (RCM) for diagnosing genital lichen sclerosus (LS). Prominent fiber-like structures observed in RCM images were able to accurately diagnose LS. RCM can be a valuable tool for monitoring LS patients for abnormal growth, providing a non-invasive diagnostic tool for genital disorders.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2022)
Article
Dermatology
Mercedes Sendin-Martin, Jasmine Posner, Ucalene Harris, Matthew Moronta, Julian Conejo-Mir Sanchez, Sushmita Mukherjee, Milind Rajadhyaksha, Kivanc Kose, Manu Jain
Summary: Basal cell carcinoma (BCC) is the most common skin cancer, and non-invasive in vivo technologies such as multiphoton microscopy (MPM) can aid in diagnosing and prognosticating these tumors. This study explored the potential of MPM to differentiate collagen changes associated with different BCC subtypes compared to normal skin structures and benign lesions. The results showed that collagen distribution is more aligned surrounding BCCs nests compared to normal structures and benign lesions, and collagen is orientated more parallelly surrounding indolent BCC subtypes versus those with aggressive behavior.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Cell Biology
Svetlana Rodimova, Nikolai Bobrov, Artem Mozherov, Vadim Elagin, Maria Karabut, Ilya Shchechkin, Dmitry Kozlov, Dmitry Krylov, Alena Gavrina, Vladimir Zagainov, Elena Zagaynova, Daria Kuznetsova
Summary: In order to reduce the risk of post-hepatectomy liver failure in patients with hepatic pathologies, a method to assess the liver's regenerative potential during surgery is necessary. Traditional clinical methods are not effective in predicting the function of the liver remnant. Modern label-free bioimaging techniques, such as multiphoton microscopy combined with second harmonic generation and fluorescence lifetime imaging microscopy, can provide insights into liver pathologies and regenerative potential. By using these techniques, we were able to identify markers for impaired regenerative potential and metabolic changes in pathological liver tissue.
Article
Biochemical Research Methods
Jiajia He, Deyong Kang, Tingfeng Shen, Liqin Zheng, Zhenlin Zhan, Gangqin Xi, Wenjiao Ren, Zhong Chen, Lida Qiu, Shuoyu Xu, Lianhuang Li, Jianxin Chen
Summary: In this study, multiphoton microscopy is used as a new method to accurately visualize the morphological features of invasive micropapillary carcinoma of the breast (IMPC). The results show that different morphologies of IMPC have different invasive abilities.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Multidisciplinary Sciences
Fouzi Benboujja, Chri Stopher Hartnick
Summary: Identifying distinct features of the vocal fold extracellular matrix is crucial for laryngeal diagnostics and therapy. Optical imaging techniques have shown promise in unraveling the complexity of vocal fold microanatomy. This study revealed a diverse fibrillar arrangement in the vocal fold ECM, challenging traditional descriptions of its structure.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Bohan Zhang, Tengteng Yao, Yaxin Chen, Chuqiao Wang, Yongyang Bao, Zhaoyang Wang, Keke Zhao, Minbiao Ji
Summary: Femtosecond time-resolved pump-probe microscopy can provide label-free and chemical-specific detection of melanin species in uveal melanoma, and delineate the interface between melanoma and paracancerous regions on various tissue conditions. Transcriptome sequencing confirms the presence of active eumelanin synthesis in uveal melanoma.
FRONTIERS IN ONCOLOGY
(2022)
Article
Dermatology
Won Yeong Park, Bumju Kim, Ji Hyun Chun, Seung-Mo Hong, Byung Ho Oh, Ki Hean Kim
Summary: The study successfully performed sensitive cancer cell detection in various skin cancer specimens using combined reflection confocal and moxifloxacin-based two-photon microscopy, effectively distinguishing between normal and cancer cell structures. This combined microscopy technique shows potential as an effective tool for skin cancer detection and in vivo guidance.
LASERS IN SURGERY AND MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Zahra Khabir, Amy M. Holmes, Yi-Jen Lai, Liuen Liang, Anand Deva, Michael A. Polikarpov, Michael S. Roberts, Andrei V. Zvyagin
Summary: The study demonstrated that zinc oxide nanoparticles are retained on the surface or within the superficial layers of the stratum corneum, and the released zinc ions penetrate into the viable epidermis as ionic zinc instead of zinc oxide nanoparticles. This finding supports the safety of zinc oxide NP sunscreens on intact human skin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Christoph Krafft, Juergen Popp, Peter Bronsert, Arkadiusz Miernik
Summary: Raman spectroscopy allows for label-free assessment of bladder tissue in intraoperative applications. In a retrospective study, Raman microspectroscopic images were used to identify different components of bladder tissue, including normal bladder, bladder cancer, necrosis, epithelium, and lipid inclusions. Additionally, foreign materials such as microplastic particles and pigments were detected in the specimens. This study demonstrates the potential of Raman spectroscopy for tumor detection and material identification in complex matrices.
Article
Biochemical Research Methods
Gangqin Xi, Chen Huang, Jie Lin, Tianyi Luo, Bingzi Kang, Mingyu Xu, Huizhen Xu, Xiaolu Li, Jianxin Chen, Lida Qiu, Shuangmu Zhuo
Summary: Lung cancer is highly prevalent in China and contributes to the majority of cancer-related deaths. This study investigates the potential of using label-free multiphoton microscopy (MPM) for imaging adenocarcinoma tissues in the lung. The researchers found that second harmonic generation (SHG) signals can effectively quantify collagen features in lung adenocarcinomas, and a collagen score was developed to distinguish between normal and tumor areas at the tumor boundary. Overall, this study demonstrates the ability of MPM imaging combined with collagen feature extraction to accurately detect early-stage lung adenocarcinoma tissues.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Biochemistry & Molecular Biology
Cornelia Peterson, Rupin N. Parikh, Meleha T. Ahmad, Ashley A. Campbell, Yassine Daoud, Nicholas Mahoney, Sepideh Siadati, Charles G. Eberhart
Summary: In-situ hybridization is a convenient and reliable method for detecting human papillomavirus infection in formalin-fixed paraffin-embedded tissue. This study found that RNA in-situ hybridization is effective in detecting HPV infection in squamous lesions of the conjunctiva and can help distinguish between reactive atypia and true dysplasia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Optics
Tomohiro Ishikawa, Keisuke Isobe, Kenta Inazawa, Kana Namiki, Atsushi Miyawaki, Fumihiko Kannari, Katsumi Midorikawa
Summary: In this study, adaptive optics (AO) correction was demonstrated in a thick sample to address wavefront distortion in temporal focusing microscopy (TFM). The use of structured illumination microscopy (SIM) assisted AO correction successfully reduced the change in pulse duration in the signal detection volume, which was challenging to correct with traditional TFM AO correction.
Article
Chemistry, Analytical
Juliana Schons Gularte, Roana de Oliveira Hansen, Meriane Demoliner, Jacek Fiutowski, Ana Karolina Antunes Eisen, Fagner Henrique Heldt, Paula Rodrigues de Almeida, Daniela Muller de Quevedo, Horst-Gunter Rubahn, Fernando Rosado Spilki
Summary: The study aimed to detect Human mastadenovirus species C (HAdV-C) and F (HAdV-F) in water samples using magnetic bead surface functionalization with immunomagnetic separation combined with real-time polymerase chain reaction (IMS-qPCR), and found that IMS was more effective than ultracentrifugation in concentrating HAdV, improving the assessment of infectious HAdV in water resources. IMS-qPCR could detect HAdV dilutions and IMS-concentrated samples were able to infect cell cultures.
Article
Cardiac & Cardiovascular Systems
Hein Heidbuchel, Paolo E. Adami, Matthias Antz, Frieder Braunschweig, Pietro Delise, Daniel Scherr, Erik E. Solberg, Matthias Wilhelm, Antonio Pelliccia
Summary: Cardiologists and sports physicians need to carefully consider the impact of arrhythmias on sports participation and provide balanced recommendations based on individual circumstances.
EUROPEAN JOURNAL OF PREVENTIVE CARDIOLOGY
(2021)
Article
Oncology
Ahmad Shabsigh, Nir Kleinmann, Angela B. Smith, Douglas Scherr, Elyse Seltzer, Mark Schoenberg, Seth P. Lerner
Summary: This study evaluated the pharmacokinetic properties of UGN-101 in treating patients with low-grade UTUC, showing that UGN-101 reverse thermal gel leads to longer duration and higher concentration of mitomycin in contact with the urothelium of the upper urinary tract while limiting systemic absorption, with good clinical safety profile.
CANCER CHEMOTHERAPY AND PHARMACOLOGY
(2021)
Article
Urology & Nephrology
Bashir Al Hussein Al Awamlh, Jonathan E. Shoag, Spyridon P. Basourakos, Patrick J. Lewicki, Line Posada, Xiaoyue Ma, Jay D. Raman, Shahrokh F. Shariat, Douglas Scherr
Summary: Factors such as black race, larger tumors, advanced tumor stage, and high-grade tumors are associated with inadvertent radical nephrectomy for upper tract urothelial carcinoma. However, patients who underwent surgery at a facility performing a higher volume of nephroureterectomy per year had lower odds of having radical nephrectomy performed. Patients who underwent radical nephrectomy had significantly worse overall survival compared to those treated with radical nephroureterectomy, highlighting the importance of accurate diagnosis in UTUC.
Article
Oncology
Siv Venkat, Patrick J. Lewicki, Spyridon P. Basourakos, Douglas S. Scherr
Summary: The study found that the overall rates of pathological complete response and downstaging were 8.74% and 29.77% respectively after multi-agent neoadjuvant chemotherapy. Node-negative and node-positive diseases had equivalent rates of complete response, but node-positive disease had a significantly higher rate of downstaging. The presence of lymphovascular invasion (LVI) was associated with worse overall survival.
Article
Oncology
Siv Venkat, Patrick J. Lewicki, Aleem Khan, Chris Gaffney, Leonardo Borregales, Douglas S. Scherr
Summary: Significant sociodemographic disparities exist in the United States in access to neoadjuvant and adjuvant chemotherapy for bladder cancer, with uninsured and Medicaid insurance status being the strongest predictors of not receiving chemotherapy.
CLINICAL GENITOURINARY CANCER
(2022)
Article
Urology & Nephrology
Surena F. Matin, Phillip M. Pierorazio, Nir Kleinmann, John L. Gore, Ahmad Shabsigh, Brian Hu, Karim Chamie, Guilherme Godoy, Scott G. Hubosky, Marcelino Rivera, Michael O'Donnell, Marcus Quek, Jay D. Raman, John J. Knoedler, Douglas Scherr, Christopher Weight, Alon Weizer, Michael Woods, Hristos Kaimakliotis, Angela B. Smith, Jennifer Linehan, Jonathan Coleman, Mitchell R. Humphreys, Raymond Pak, David Lifshitz, Michael Verni, Ifat Klein, Marina Konorty, Dalit Strauss-Ayali, Gil Hakim, Elyse Seltzer, Mark Schoenberg, Seth P. Lerner
Summary: The study aimed to evaluate the long-term safety and durability of UGN-101 as a primary chemoablative treatment for low-grade upper tract urothelial carcinoma. The results showed that UGN-101 has a clinically meaningful durability of response, providing a kidney-sparing therapeutic alternative for patients with low-grade disease.
JOURNAL OF UROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ekaterina Laukhtina, Victor M. Schuettfort, David D. Andrea, Benjamin Pradere, Keiichiro Mori, Fahad Quhal, Reza Sari Motlagh, Hadi Mostafaei, Satoshi Katayama, Nico C. Grossmann, Pawel Rajwa, Flora Zeinler, Mohammad Abufaraj, Marco Moschini, Kristin Zimmermann, Pierre Karakiewicz, Harun Fajkovic, Douglas Scherr, Eva Comperat, Peter Nyirady, Michael Rink, Dmitry Enikeev, Shahrokh F. Shariat
Summary: Elevated preoperative plasma endoglin levels are associated with adverse pathological features and survival outcomes in patients with urothelial carcinoma of the bladder, indicating its potential as a predictor for intensified therapy benefit.
MOLECULAR CARCINOGENESIS
(2022)
Article
Oncology
Siv Venkat, Aleem Khan, Patrick J. Lewicki, Leonardo Borregales, Douglas S. Scherr
Summary: Accurate preoperative nomograms were developed to predict muscle-invasive disease and lymph node metastasis in upper tract urothelial carcinoma (UTUC). These nomograms, based on factors such as age, tumor grade, lymphovascular invasion (LVI) status, tumor size, and clinical lymph node status, can assist surgeons in risk stratifying patients and guiding treatment decisions.
UROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONS
(2022)
Article
Oncology
Siv Venkat, Aleem Khan, Benjamin L. Taylor, Neal A. Patel, Bashir Al Hussein Al Awamlh, Lina Posada Calderon, Jonathan Fainberg, Jonathan Shoag, Douglas S. Scherr
Summary: The use of neoadjuvant chemotherapy (NAC) reduces the sex-specific survival disparities in patients with advanced bladder cancer, although female patients still have poorer survival outcomes in the absence of NAC. Strategies should be implemented to improve the utilization of NAC in women to overcome potential sex-specific differences in diagnosis, disease stage, or tumor biology that contribute to differences in survival outcomes.
UROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONS
(2022)
Article
Medicine, Research & Experimental
Jacqueline Fontugne, Peter Y. Cai, Hussein Alnajar, Bhavneet Bhinder, Kyung Park, Huihui Ye, Shaham Beg, Verena Sailer, Javed Siddiqui, Mirjam Blattner-Johnson, Jaclyn A. Croyle, Zohal Noorzad, Carla Calagua, Theresa Y. MacDonald, Ulrika Axcrona, Mari Bogaard, Karol Axcrona, Douglas S. Scherr, Martin G. Sanda, Bjarne Johannessen, Arul M. Chinnaiyan, Olivier Elemento, Rolf Skotheim, Mark A. Rubin, Christopher E. Barbieri, Juan Miguel Mosquera
Summary: Prostate cancer is a multifocal disease with distinct molecular subtypes. This study reveals the presence of inter- and intrafocal molecular heterogeneity in primary prostate cancer, with approximately 60% of multifocal specimens showing discordant molecular alterations between foci. Collision tumors, characterized by discrepant molecular status within a single focus, are identified in about 10% of the specimens. These findings highlight the complexity of molecular heterogeneity in prostate cancer and have important implications for future genomic studies.
Letter
Medicine, General & Internal
Patrick Lewicki, Spyridon P. Basourakos, Camilo Arenas-Gallo, Yuqing Qiu, Megan Prunty, Douglas S. Scherr, Jonathan E. Shoag
Meeting Abstract
Medicine, Research & Experimental
Muhammad Asad, Peter Cai, Francesca Khani, Michael Augello, Maria Laura Martin, Christopher Louie, Spyridon Basourakos, Christopher Gaffney, Jonathan Shoag, Douglas Scherr, Juan Miguel Mosquera, Christopher Barbieri, Brian Robinson
LABORATORY INVESTIGATION
(2021)
Meeting Abstract
Pathology
Muhammad Asad, Peter Cai, Francesca Khani, Michael Augello, Maria Laura Martin, Christopher Louie, Spyridon Basourakos, Christopher Gaffney, Jonathan Shoag, Douglas Scherr, Juan Miguel Mosquera, Christopher Barbieri, Brian Robinson
Review
Surgery
Hudson Pierce, Brittany Roberts, Douglas Scherr, Christopher Barbieri, Jennifer Anger, Timothy McClure, Bilal Chughtai
Summary: Robotic prostatectomy is the most common robotic procedure in the US, and a review of adverse events involving the intuitive surgical system found that malfunctions, mostly instrument failures, are the main issue during the procedure. Despite the high number of malfunctions reported, they are usually recoverable and rarely result in patient injury.
JOURNAL OF ROBOTIC SURGERY
(2021)
Article
Cardiac & Cardiovascular Systems
D. Scherr, M. Manninger-Wunscher
JOURNAL FUR KARDIOLOGIE
(2020)