Article
Immunology
Min Chen, Pei Yang, Zixuan Xin, Jiating Chen, Weihao Zou, Lijuan Zhou, Lili Yang, Jiao Peng, Hongjuan Peng
Summary: Toxoplasma gondii is a pathogen causing toxoplasmosis and has similarities with immunotherapy for cancers. The knockout of GRA5 gene in T. gondii resulted in an avirulent strain that stimulated immune responses. ME49 & UDelta;gra5 vaccination provided protection against T. gondii infection and breast cancer by boosting anti-tumor responses. The vaccine upregulated Th1 cytokines and tumor-infiltrating T cells, and increased the number of immune cells in the spleen.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Jian He, Yongheng Hou, Fangli Lu
Summary: The interaction between Galectin-9 and its receptors plays an important regulatory role in anti-toxoplasmic activities. Blocking Galectin-receptor interactions can improve the survival rate of mice infected with Toxoplasma gondii, reduce liver histopathology, and increase the expression levels of CD137, IFN gamma, IL-4, and IL-10 in the liver.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Iti Saraav, Luisa Cervantes-Barragan, Philipp Olias, Yong Fu, Qiuling Wang, Leran Wang, Yi Wang, Matthias Mack, Megan T. Baldridge, Thaddeus Stappenbeck, Marco Colonna, L. David Sibley
Summary: Oral infection with Toxoplasma gondii exacerbates damage to the colon caused by chemical irritant and impairs wound healing by suppressing stem cell regeneration. Enhanced tissue damage is due to inflammatory monocytes releasing inflammatory mediators.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Ting-Ting Li, Dan-Yu Zhao, Qin-Li Liang, Hany M. Elsheikha, Meng Wang, Li-Xiu Sun, Zhi-Wei Zhang, Xiao-Qing Chen, Xing-Quan Zhu, Jin-Lei Wang
Summary: This study focuses on the roles of glutaredoxins (Grxs) in the pathogenicity of Toxoplasma gondii. The researchers found that grx1 plays a crucial role in protecting the parasite against oxidative killing and is essential for its growth in cultured cells and pathogenicity in mice.
Review
Parasitology
Rajshekhar Y. Gaji, Amanda K. Sharp, Anne M. Brown
Summary: Toxoplasma gondii is a life-threatening intracellular pathogen that depends on protein kinases for its life cycle. Research on Toxoplasma kinases aims to understand their functions and potentially develop therapeutics against this parasite.
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2021)
Article
Parasitology
Anamaria Ioana Pastiu, Viorica Mircean, Aurelien Mercier, Karine Passebosc-Faure, Nicolas Plault, Marie-Laure Darde, Radu Blaga, Isabelle Villena, Dana Liana Pusta, Anamaria Cozma-Petrut, Adriana Gyoerke
Summary: This study investigated the epidemiology of Toxoplasma gondii infection in sheep in Romania and successfully isolated the infective strains. The results showed a high prevalence of T. gondii infection in sheep and lambs, highlighting the significant risk of human infection through consuming raw or undercooked sheep/lamb meat.
PARASITES & VECTORS
(2023)
Article
Immunology
Jing-Mei Lu, Xiang Xu, Fumie Aosai, Ming-Yue Zhang, Lian-Xun Piao
Summary: This study found that arctiin can improve allergic acute liver injury caused by T.g.HSP70 by inhibiting TLR4 signaling and reducing the production of inflammatory mediators.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2024)
Article
Engineering, Multidisciplinary
Wen Jiang, Mengyue Wang
Summary: Through studying the genotype of Toxoplasma gondii, it was found that there are genetic differences in Central and South America. From the perspective of pathogen virulence evolution, it is discovered that transmission route is a key factor causing global genetic differences in Toxoplasma gondii, and the diversity of hosts does not always increase its virulence differences. The reproductive mode of Toxoplasma gondii and frequent human trades are prerequisites for the development of its virulence.
APPLIED MATHEMATICAL MODELLING
(2023)
Article
Microbiology
Cristiana Luiza Radoi, Ovidiu Mircea Zlatian, Maria Balasoiu, Lucian Giubelan, Andreea Cristina Stoian, Livia Dragonu, Alexandru Neacsu, Dominic Gabriel Iliescu
Summary: This study examined the prevalence of anti-T. gondii IgM and IgG antibodies in two groups of childbearing age women. The results showed a decrease in the overall positivity rate of anti-T. gondii IgM antibodies, indicating improved public health interventions. However, there were variations among different age groups and between rural and urban environments, with a significant decrease in urban areas.
Article
Microbiology
Farlen J. B. Miranda, Bruno C. Rocha, Milton C. A. Pereira, Larissa M. N. Pereira, Erikson H. M. de Souza, Ana P. Marino, Pedro A. C. Costa, Daniel V. Vasconcelos-Santos, Lis R. Antonelli, Ricardo T. Gazzinelli
Summary: Toxoplasmosis affects a significant portion of the global population, with humans becoming accidental hosts through consumption of undercooked meat and contaminated water. Neutrophils play a crucial role in controlling T. gondii infections, with their activation and formation of NETs amplifying both innate and adaptive immune responses.
Review
Immunology
Chanjin Yoon, Yu Seong Ham, Woo Jin Gil, Chul-Su Yang
Summary: This review article provides an overview of the activation and specific role of the NLRP3 inflammasome in Toxoplasma gondii infection. It points out that the role of NLRP3 inflammasome in T. gondii infection is still uncertain and the mechanisms of its activation in different cell types are still unknown.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Food Science & Technology
Pablo-Jesus Marin-Garcia, Nuria Planas, Lola Llobat
Summary: This article reviews the detection methods, prevalence, and control measures of Toxoplasma gondii in the food industry. T. gondii is a common parasite that causes infection through food contamination, but standardized detection methods are currently lacking in the industry. The main detection methods include bioassays, cell culture, molecular and microscopic techniques, and serological methods. Due to the high prevalence of T. gondii in many food products, standardized detection methods and control measures are necessary.
Article
Immunology
Hong-chao Sun, Pu-ming Deng, Yuan Fu, Jin-hua Deng, Rong-hui Xie, Jing Huang, Meng Qi, Tuan-yuan Shi
Summary: In this study, PLGA nanoparticles were used as a delivery system for T. gondii GRA12 and GRA7 antigens. Mice immunized with PLGA+GRA12 showed higher levels of IgG antibodies, increased lymphocyte proliferation, and a predominant Th1 immune response. Immunization with PLGA+GRA12 vaccine resulted in significantly reduced parasite loads and longer survival times compared to other vaccinated groups. However, PLGA was ineffective in enhancing the immune response induced by T. gondii GRA7.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Jing-Mei Lu, Guang-Nan Jin, Yu-Nan Lu, Xu-Dong Zhao, Hui-Wen Lan, Shuai-Ru Mu, Xin-Yu Shen, Guang-Hua Xu, Cheng-Hua Jin, Juan Ma, Xuejun Jin, Xiang Xu, Lian-Xun Piao
Summary: The study demonstrates that resveratrol can protect liver injury induced by T. gondii infection by intervening in the HMGB1/TLR4/NF-kappa B signaling pathway.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Microbiology
Tadakimi Tomita, Debanjan Mukhopadhyay, Bing Han, Rama Yakubu, Vincent Tu, Joshua Mayoral, Tatsuki Sugi, Yanfen Ma, Jeroen P. J. Saeij, Louis M. Weiss
Summary: The study reveals that the cyst matrix protein MAG1 functions as a secreted immunomodulatory effector, regulating parasite virulence and dissemination by suppressing inflammasome activation. The balance between GRA15-induced inflammasome activation and MAG1-mediated suppression plays a crucial role in host-parasite interactions and the establishment of chronic infection.
Article
Parasitology
Fagner D'ambroso Fernandes, Renata Rojas Guerra, Ananda Segabinazzi Ries, Juliana Felipetto Cargnelutti, Luis Antonio Sangioni, Fernanda Silveira Flores Vogel
Summary: This study aimed to identify antiparasitic-resistant helminths and evaluate the infection rate and risk factors for parasitism in canines. The results showed a high prevalence of Ancylostoma caninum infection in dogs, and variations in the efficacy of different drugs. The environment and breed of the dogs were found to be important risk factors for infection.
PARASITOLOGY RESEARCH
(2022)
Article
Parasitology
Bibiana Rodrigues de Freitas, Isac Junior Roman, Bibiana Rossi Reis, Fernando de Souza Rodrigues, Patricia Brauenig, Juliana Felipetto Cargnelutti, Rodrigo Casquero Cunha, Fernanda Silveira Flores Vogel
Summary: The purpose of this study was to detect visible cysts of Sarcocystis in cattle hearts from slaughterhouses and evaluate the viability of sarcocysts after freezing treatment. Cysts were observed in all analyzed tissues, with a density of 21.73 cysts/g of tissue. Freezing treatment at -35℃ for ≥3 h and -20℃ for ≥8 h rendered the cysts and bradyzoites unviable.
ACTA PARASITOLOGICA
(2023)
Article
Agronomy
Gabriela Dowich Pradella, Claudia Acosta Duarte, Luisa Zuravski, Taiane Acunha Escobar, Roberto Thiesen, Mario Celso Sperotto Brum, Isac Junior Roman, Fernanda Silveira Flores Vogel, Irina Lubeck
Summary: Leishmaniosis is a serious public health problem affecting both humans and animals. In this study, an in-house indirect ELISA method was evaluated for the detection of immune response to Leishmania spp. in canines. The ELISA technique showed high sensitivity and specificity compared to other methods and was valuable for screening tests in endemic areas.
Article
Parasitology
Fagner D. 'ambroso Fernandes, Patricia Braunig, Bruna Marquardt Lucio, Maiana Martins Flores, Cinthia Melazzo de Andrade, Fernanda Silveira Flores Vogel
Summary: This study aimed to detect anti-Sarcocystis spp.-specific antibodies in domestic cats and found that 4.82% of the cat serum samples tested positive. The presence of these antibodies confirms the occurrence of natural Sarcocystis infections in cats and highlights their role as hosts in the parasite's life cycle.
PARASITOLOGY RESEARCH
(2023)
Review
Parasitology
Fabiana Raquel Ratzlaff, Vanessa Osmari, Daniele da Silva, Jaine Soares de Paula Vasconcellos, Luciana Potter, Fagner D'ambroso Fernandes, Jose Americo de Mello Filho, Sonia de Avila Botton, Fernanda Silveira Flores Vogel, Luis Antonio Sangioni
Summary: This systematic review identified the host species of Leishmania spp. in Brazil, excluding domestic dogs. Horses, domestic cats, rodents, and marsupials were found to be the most commonly infected species, while bats were identified as potential reservoirs. Molecular tests were frequently used for diagnosis. Understanding the species involved in the epidemiology and biological cycle of Leishmania can improve control measures for zoonotic leishmaniasis.
PARASITOLOGY RESEARCH
(2023)
Article
Parasitology
Leticia da Silva Giacomini, Fagner D'ambroso Fernandes, Renata Rojas Guerra, Sonia de Avila Botton, Luis Antonio Sangioni, Fernanda Silveira Flores Vogel
Summary: The objective of this study was to evaluate the effectiveness of a commercial vaccine against avian coccidiosis. The results showed that the disease has a significant impact on the productivity and economy of broiler chickens, and vaccination can prevent the disease and reduce subsequent losses.
PARASITOLOGY RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Ticiane da Rosa Pinheiro, Gabrielle Aguiar Dantas, Jean Lucas Gutknecht da Silva, Daniela Bitencourt Rosa Leal, Ricardo Barreto da Silva, Thiago Augusto de Lima Burgo, Roberto Christ Vianna Santos, Bernardo Almeida Iglesias
Summary: This study evaluated the in vitro activity of cationic porphyrins with platinum(II) complexes against Candida albicans. The results showed that 3PtTPyP exhibited excellent antifungal activity and reduced preformed biofilms effectively.
Article
Cell Biology
Pedro Henrique Doleski, Fernanda Licker Cabral, Matheus Henrique Jantsch, Renan Silva Ebone, Stephen Adeniyi Adefegha, Daniela Bitencourt Rosa Leal, Maria Rosa Chitolina Schetinger
Summary: Plasma membrane anchored nucleotidases (E-ATPDases) play an important role in the hydrolysis and regulation of nucleotides in lymphocytes. The activities of E-ATPDases vary in different immune organs and resident subpopulations of lymphocytes. This study characterizes the E-ATPDases activities in bone marrow, thymus, and mesenteric lymph node lymphocytes of Wistar rats. The results suggest that the kinetic values of E-NTPDase activity in immune tissue lymphocytes could be an important therapeutic target for immune-related diseases.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Jessica Brandao Reolon, Camila Parcianello Saccol, Barbara Felin Osmari, Daiane Britto de Oliveira, Vinicius Costa Prado, Fernanda Licker Cabral, Lucas Saldanha da Rosa, Giancarlo Cervo Rechia, Daniela Bitencourt Rosa Leal, Leticia Cruz
Summary: This study aimed to develop a bilayer film for topical melanoma therapy by incorporating nanocapsules containing 3,3 '-diindolylmethane (DIM) with antitumor activity. The nanofilms showed appropriate behavior for cutaneous application and controlled DIM release profile, and increased the permeation of DIM into the epidermis. The combination of karaya and gellan gum in the bilayer films demonstrated potential in the topical treatment of melanoma.
Article
Parasitology
Bibiana Rodrigues de Freitas, Gilneia da Rosa, Isac Junior Roman, Leticia Trevisan Gressler, Rodrigo Casquero Cunha, Juliana Felipetto Cargnelutti, Fernanda Silveira Flores Vogel
Summary: The aim of this study was to determine the presence of DNA from three protozoa in tissues of wild boars slaughtered in southern Brazil. The DNA of at least one of the studied pathogens was detected in each animal, with Toxoplasma gondii being the most prevalent. Coinfection between different pathogens was also observed. Considering the high frequency of detection and the zoonotic risk, it is important to prioritize monitoring measures for wild boar populations.
REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA
(2023)
Article
Veterinary Sciences
Maiara S. T. Ferreira, Fagner D'ambroso Fernandes, Patricia Braeunig, Renata R. Guerra, Luis Antonio Sangioni, Fernanda S. F. Vogel
Summary: This study investigated the detection of Sarcocystis spp. infection in cattle using fresh microscopic examination, PCR, and serological methods. The results showed that microscopic Sarcocystis were observed in 100% of the myocardial samples, and Sarcocystis DNA was present in 86% of these samples. Antibodies against Sarcocystis spp. were detected in 96% and 80% of the serum samples at 1:25 and 1:200 dilutions respectively. The combination of these three methods showed good sensitivity and detection for Sarcocystis spp., providing a useful approach for diagnosing live animals and monitoring the herd status on a large scale.
PESQUISA VETERINARIA BRASILEIRA
(2023)
Article
Oncology
Jean Gutknecht L. da Silva, Daniela F. Passos, Fernanda L. Cabral, Vanessa V. Miron, Maria R. C. Schetinger, Andrieli A. Cardoso, Camile H. Dal Piva, Carolina O. Gomes, Renan S. Ebone, Daniela B. R. Leal
Summary: This study aimed to investigate the effects of A2AR antagonism with IST on purinergic signaling in melanoma tumor and immune compartments. The results showed that IST inhibited AKT/mTOR pathway, leading to reduced tumor growth. It also promoted the production of pro-inflammatory cytokines, suggesting IST as a promising drug for cancer treatment.
Article
Parasitology
Mauricio Tatto, Fagner D'ambroso Fernandes, Eliesse Pereira Costa, Fabio Yuji Shibuya, Luiza Isaia de Freitas, Vanessa Osmari, Isac Junior Roman, Patricia Braunig, Fernanda Silveira Flores Vogel, Sonia de Avila Botton, Luis Antonio Sangioni
Summary: This study aimed to detect the presence of anti-Leishmania spp. antibodies in domestic birds from a rural area in southern Brazil. The results showed that all the rural areas sampled had positive birds for the presence of Leishmania spp. This suggests that domestic birds can be used as sentinels for the circulation of this parasite, and their blood can serve as a preferred food source for the insects that transmit the disease.
REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA
(2023)
Article
Veterinary Sciences
Gabriela Tormes Nunes, Diego Cristiano Correa, Maysa Bigolin Chitolina, Gilneia da Rosa, Roberta Carneiro da Fontoura Pereira, Juliana Felipetto Cargnelutti, Fernanda Silveira Flores Vogel
Summary: This study evaluated the in vitro efficacy of the commercial formulation Bioverm in reducing gastrointestinal nematode larvae in equine feces. The formulation demonstrated effectiveness in predating Cyathostomum sp. and controlling infective larvae.
JOURNAL OF EQUINE VETERINARY SCIENCE
(2023)
Article
Agronomy
Camila Tochetto, Guilherme Augusto Marietto Goncalves, Diogo Souza Zanoni, Renato Lemos Pereira, Gisele Torres Climaco De Araujo, Renan Mori Rocha, Bruna Carolina Ulsenheimer, Alexandre Alberto Tonin
Summary: This is a case report on a captive female jaguar in the Amazonas region of Brazil. The 12-year-old jaguar presented with anorexia and lethargy, and post-mortem examination revealed abdominal multinodular ventral plaque and multiple metastatic nodules. The diagnosis of metastatic anaplastic mammary carcinoma was confirmed through histopathological examination.
Article
Pathology
Xiaobo Zhang, Chen Wang, Danhua Shen
Summary: This study investigated the Clinicopathological features and FH mutation in FH-dUL. FH-dUL is rare and the combination of predictive Clinicopathological evaluation, FH and 2SC IHC test, and molecular test were helpful for the screening of FH-dUL or even HLRCC.
PATHOLOGY RESEARCH AND PRACTICE
(2024)
Review
Pathology
Gaurav Dubey, Mithilesh Singh, Himmat Singh, Mohit Agarwal, Shailendra Singh Chandel, Anurag Mishra, Ravindra Pal Singh, Neelima Kukreti
Summary: SnoRNAs play a crucial role in autoimmune diseases, influencing gene expression, immune cell development, and immune regulation. They have the potential to be biomarkers or therapeutic targets for disease management. However, further research is needed to elucidate their precise molecular mechanisms.
PATHOLOGY RESEARCH AND PRACTICE
(2024)
Article
Pathology
P. Suhaj, D. Do, T. Olejar, R. Pichova, O. Lang, R. Matej
Summary: Pancreatic polypeptide cell hyperplasia (PPY-H) is a multiplication of neuroendocrine cells producing pancreatic polypeptide (PPY). The development and role of PPY-H still need to be further elucidated. This study analyzed 12 cases of PPY-H accompanying pancreatic neuroendocrine neoplasias (NEN) and found that gastrointestinal symptoms may be more related to PPY-H rather than NEN hormonal production. Strong SSTR2 expression in PPY-H suggests the potential utility of radiotracers in clinical diagnostics, but further studies are needed.
PATHOLOGY RESEARCH AND PRACTICE
(2024)
Article
Pathology
Ruyun Zhang, Xiaofei Liao, Bin Zhang, Xiaohong Huang, Guanjie Qin, Xiangyun Kong, Yuan Xie, Yunyan Mo, Jinxuan Dai, Chunqiao Gan, Zan Luo, Jingyan Lu, Wei Jiang
Summary: This study found that patients with high COV+TIL score had worse prognosis in nasopharyngeal carcinoma.
PATHOLOGY RESEARCH AND PRACTICE
(2024)
Review
Pathology
Waleed Hassan Almalki
Summary: Understanding the molecular pathways behind cardiovascular illnesses is crucial. Recent studies have revealed the role of Xist RNA in cardiovascular diseases, including epigenetic changes, gene expression, cellular identity, and sex chromosomal inactivation. Dysregulation of Xist RNA expression is associated with conditions such as atherosclerosis, hypertrophy, and cardiac fibrosis.
PATHOLOGY RESEARCH AND PRACTICE
(2024)
Article
Pathology
Yanxin Dong, Boshi Fan, Mingyang Li, Jiale Zhang, Shun Xie, Shouyin Dia, Qingge Jia, Taiqian Gong
Summary: Tescalcin (TESC) is upregulated in esophageal squamous carcinoma (ESCC) and is associated with poor prognosis, tumor infiltration, staging, and lymph node metastasis. TESC promotes proliferation, invasion, migration, and epithelial-mesenchymal transition (EMT) progression in ESCC, as well as activates the AKT pathway. TESC may serve as a prognostic factor and provide a new therapeutic strategy for ESCC.
PATHOLOGY RESEARCH AND PRACTICE
(2024)
Article
Pathology
Ozge Sukruoglu Erdogan, Demet Akdeniz Odemis, Zubeyde Yalniz Kayim, Orkun Gurbuz, Seref Bugra Tuncer, Seda Kilic, Betul Celik, Samuray Tuncer, Sema Buyukkapu Bay, Rejin Kebudi, Hulya Yazici
Summary: This study found that methylation of the RB1 gene promoter does not influence the hereditary transmission of familial retinoblastoma.
PATHOLOGY RESEARCH AND PRACTICE
(2024)
Review
Pathology
Syam Mohan, Mohammed Ageeli Hakami, Hamad Ghaleb Dailah, Asaad Khalid, Asim Najmi, Khalid Zoghebi, Maryam A. Halawi, Thaifallah Munahi Alotaibi
Summary: Cancer is a complex disease caused by multiple factors, and miR-155 plays a significant role as a regulator of the NF-kappa B signaling pathway, contributing to cancer development and progression.
PATHOLOGY RESEARCH AND PRACTICE
(2024)
