Article
Engineering, Biomedical
Ho-Ying Wan, Rita Lih Ying Shin, Jack Chun Hin Chen, Marisa Assuncao, Dan Wang, Susie K. Nilsson, Rocky S. Tuan, Anna Blocki
Summary: The study demonstrated that supplementation of Dextran sulfate (DxS) in osteogenically induced cultures of human mesenchymal stem cells (MSCs) amplified the deposition of osteoblastic extracellular matrix (ECM). This enhancement led to increased collagen type I deposition and osteogenic gene expression, as well as enrichment in bone morphogenetic protein 2 (BMP-2), suggesting a potential role of DxS in promoting osteogenic differentiation by MSCs.
ACTA BIOMATERIALIA
(2022)
Article
Cell Biology
Ali Salehi, Mohammad A. Mobarhan, Javad Mohammadi, Hosein Shahsavarani, Mohammad Ali Shokrgozar, Atefeh Alipour
Summary: The study investigated the characteristics of decellularized cabbage leaves and their potential for promoting bone differentiation, as demonstrated by enhanced osteogenic differentiation of BM-MSCs on the scaffold. These results suggest that decellularized cabbage leaves have great potential for tissue engineering applications in bone regeneration.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Review
Engineering, Biomedical
Xiurui Zhang, Yuwei Liu, Karen L. Clark, Anne-Marie Padget, Peter G. Alexander, Jianwu Dai, Weimin Zhu, Hang Lin
Summary: Mesenchymal stem cell-derived extracellular matrix (mECM) is gaining attention in tissue engineering due to its bioactivity, biocompatibility, and potential for autologous tissue engineering. However, current mECM methods have limitations such as low mechanical properties and lack of internal architecture. This review summarizes the development of mECM, recent methods to improve its properties, and proposes future studies for its application in regenerating tissues in humans.
BIOMEDICAL MATERIALS
(2021)
Article
Anatomy & Morphology
Aida Nahumi, Maryam Peymani, Asadollah Asadi, Arash Abdolmaleki, Yassin Panahi
Summary: Tissue engineering research has focused on the characteristics and biocompatibility of decellularized sheep tracheal scaffolds, as well as the differentiation of Adipose-derived stem cells (AD-MSCs) into tracheal cells. The prepared scaffolds show potential for engineering applications and provide a suitable environment for stem cell differentiation. This research serves as a foundation for future studies on tracheal decellularization scaffolds and paves the way for their use in organ regeneration and clinical medicine.
Article
Materials Science, Ceramics
Patricia Ros-Tarraga, Carlos M. Martinez, Miguel A. Rodriguez, Piedad N. De Aza
Summary: The elderly population is increasing, leading to an increase in bone and joint problems. Scaffold for bone tissue regeneration can solve these problems, and material selection is crucial for satisfactory results. This study designed a three-dimensional porous ceramic scaffold and analyzed its biocompatibility and osteoinductive capacity. The results suggest that it has potential applications as bone substitutes.
CERAMICS INTERNATIONAL
(2022)
Article
Medicine, Research & Experimental
Yamini Chandramohan, Kavya Jeganathan, Sanjana Sivanesan, Pavani Koka, Trikkur Madom Seetaraman Amritha, Selvaraj Vimalraj, Anuradha Dhanasekaran
Summary: This study successfully isolated mesenchymal stem cells (MSCs) from human Ovarian Follicular Fluid (OFF) for the first time, and characterized them through various methods. The growth of MSCs with the conditioned medium obtained from a biocomposite scaffold promoted osteoblast differentiation, indicating good culture conditions and applicability.
Article
Biotechnology & Applied Microbiology
Guillaume Rougier, Louis Maistriaux, Lies Fieve, Daela Xhema, Robin Evrard, Julie Manon, Raphael Olszewski, Fabien Szmytka, Nicolas Thurieau, Jean Boisson, Natacha Kadlub, Pierre Gianello, Catherine Behets, Benoit Lengele
Summary: This paper investigates the possibility of creating a living bone allograft using the perfusion/decellularization/recellularization (PDR) technique. The results show that the decellularized vascularized bone grafts successfully preserve the 3D morphology and extracellular matrix (ECM) microarchitecture, promote cell growth and differentiation, and offer new perspectives for repairing large bone defects and bone tissue engineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Yanqing Lu, Jingzhi Li, Nan Hou, Li Zhou, Xiaoxuan Quan, Ying Tang, Xiaoming Luo, Shi Huang, Ruina Ma
Summary: Decellularized tympanic membrane scaffold with bone marrow mesenchymal stem cells showed retained anatomical structure and low immunogenicity. Recellularized TM patch displayed natural ultrastructure suitable for cell migration and proliferation, with potential for TM reconstruction.
Article
Chemistry, Multidisciplinary
Ryan Y. Nguyen, Aidan T. Cabral, Alejandro Rossello-Martinez, Alessandro Zulli, Xiangyu Gong, Qiuting Zhang, Jing Yan, Michael Mak
Summary: This study develops a collagen hydrogel that induces the formation of heterogeneous mesoscopic architectures, known as collagen islands. These island-containing gels have highly tunable inclusions and mechanical properties, with regional enrichment in collagen concentration at the cell-scale. The collagen island architectures are used to investigate mesenchymal stem cell behavior and demonstrate alterations in cell migration and osteogenic differentiation. Moreover, induced pluripotent stem cells cultured in island-containing gels show sufficient architecture to induce mesodermal differentiation. Overall, this work highlights the importance of complex mesoscopic tissue architectures as bioactive cues in regulating cell behavior and presents a novel collagen-based hydrogel for tissue engineering applications.
ADVANCED MATERIALS
(2023)
Article
Engineering, Biomedical
Hidenori Tani, Eiji Kobayashi, Shinomi Yagi, Keisuke Tanaka, Kotaro Kameda-Haga, Shinsuke Shibata, Nobuko Moritoki, Kaworu Takatsuna, Taijun Moriwaki, Otoya Sekine, Tomohiko C. Umei, Yuika Morita, Yusuke Soma, Yoshikazu Kishino, Hideaki Kanazawa, Jun Fujita, Shunji Hattori, Shugo Tohyama
Summary: This study found that heart-derived collagen is more effective in heart tissue engineering and can lead to more mature engineered heart tissue.
Article
Cell Biology
Sandra Stamnitz, Agnieszka Krawczenko, Urszula Szalaj, Zaneta Gorecka, Agnieszka Antonczyk, Zdzislaw Kielbowicz, Wojciech Swieszkowski, Witold Lojkowski, Aleksandra Klimczak
Summary: FGF-2 and BMP-2 enhance the osteogenic differentiation potential of MSCs grown on a scaffold, making them promising tools for bone regeneration. This was confirmed through in vitro and in vivo experiments.
Article
Engineering, Biomedical
Salma Abolgheit, Sally Abdelkader, Moustafa Aboushelib, Enas Omar, Radwa Mehanna
Summary: The study demonstrated that collagen chitosan scaffolds enriched with either BM-MSCs or their EVs accelerated skin wound healing, increased macrophage count, improved collagen deposition and alignment, and enhanced the tensile strength of healed skin. EVs showed higher levels of collagen deposition and better alignment compared to the MSCs treated group.
JOURNAL OF BIOMATERIALS APPLICATIONS
(2021)
Review
Biotechnology & Applied Microbiology
Qian Zhang, Yixin Hu, Xuan Long, Lingling Hu, Yu Wu, Ji Wu, Xiaobing Shi, Runqi Xie, Yu Bi, Fangyuan Yu, Pinxue Li, Yu Yang
Summary: Cartilage regeneration relies on cellular-ECM interactions. Traditional tissue engineering methods have not been able to replicate the physiological structure of natural cartilage, but recent advances in ECM-based scaffolds show promise in improving cartilage regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Engineering, Biomedical
Xiaojie Liu, Tao Fang, Ting Shi, Yun Wang, Guanying Liu
Summary: The anatomical and physiological architecture of the craniofacial bone is complex, making precise management of osteogenesis crucial for regeneration of any deficiencies in this area. Stem-based tissue engineering approaches offer a safer and more cost-effective way to induce bone growth compared to traditional surgical intervention. Mesenchymal stem/stromal cells (MSCs) have pluripotent differentiation potential, anti-inflammatory and immunomodulatory properties that make them versatile in bone tissue therapies. Hydrogels, with their excellent swelling capabilities and similarity to natural extracellular matrices, are preferred choices for mediating cell growth and adapting to 3D environments. Due to their biocompatibility and ability to stimulate bone regeneration, hydrogels have become a topic of great interest in bone tissue engineering. This review explores the potential of MSC-based regenerative skeletal therapies and the application of hydrogel scaffolds as artificial bone microenvironments for stem cells in craniofacial bone tissue engineering.
JOURNAL OF BIOMATERIALS APPLICATIONS
(2023)
Article
Biophysics
Lin Tang, Xiaoying Chen, Mei Wang, Yuhua Liu, Bowen Li, Yuke Li, Yi Zhang
Summary: This study explores the construction of an organic-inorganic composite scaffold for bone regeneration, using decellularized small intestinal submucosa (SIS) as the organic component and phosphorylated hydroxyapatite nanocrystals (nHA) as the inorganic component. The nHA@SIS scaffold showed superior physicochemical properties and osteogenic ability compared to the SIS scaffold, indicating great potential for bone tissue engineering.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Cell & Tissue Engineering
Jitske Jansen, Katharina C. Reimer, James S. Nagai, Finny S. Varghese, Gijs J. Overheul, Marit de Beer, Rona Roverts, Deniz Daviran, Liline A. S. Fermin, Brigith Willemsen, Marcel Beukenboom, Sonja Djudjaj, Saskia von Stillfried, Larissa E. van Eijk, Mirjam Mastik, Marian Bulthuis, Wilfred den Dunnen, Harry van Goor, Jan-Luuk Hillebrands, Sergio H. Triana, Theodore Alexandrov, Marie-Cherelle Timm, Bartholomeus T. van den Berge, Martijn van den Broek, Quincy Nlandu, Joelle Heijnert, Eric M. J. Bindels, Remco M. Hoogenboezem, Fieke Mooren, Christoph Kuppe, Pascal Miesen, Katrien Grunberg, Ties Ijzermans, Eric J. Steenbergen, Jan Czogalla, Michiel F. Schreuder, Nico Sommerdijk, Anat Akiva, Peter Boor, Victor G. Puelles, Juergen Floege, Tobias B. Huber, Ronald P. van Rij, Ivan G. Costa, Rebekka K. Schneider, Bart Smeets, Rafael Kramann
Summary: The study found that SARS-CoV-2 can directly infect kidney cells, leading to cell injury and fibrosis, causing kidney failure. Inhibitors can alleviate the infection.
Article
Hematology
Roberto Avellino, Roger Mulet-Lazaro, Marije Havermans, Remco Hoogenboezem, Leonie Smeenk, Nathan Salomonis, Rebekka K. Schneider, Elwin Rombouts, Eric Bindels, Lee Grimes, Ruud Delwel
Summary: The transcription factor C/EBPa plays a key role in initiating the gene expression program of neutrophils in the bone marrow. Knockouts of the Cebpa gene or its +37kb enhancer in mice result in decreased neutrophil count and reduced numbers of long-term hematopoietic stem cells (LT-HSCs). The reduction in LT-HSC numbers is proportional to the degree of neutropenia, suggesting an extrinsic effect. Transplantation experiments demonstrated that deletion of the +37kb enhancer leads to cell-autonomous neutropenia and disruption of HSC quiescence, impairing the hematopoietic process systemically.
Article
Hematology
Ursula S. A. Stalmann, Fabio Ticconi, Inge A. M. Snoeren, Ronghui Li, Helene F. E. Gleitz, Glenn S. Cowley, Marie E. McConkey, Aaron B. Wong, Stephani Schmitz, Stijn N. R. Fuchs, Shubhankar Sood, Nils B. Leimkuehler, Sergio Martinez-Hoyer, Bella Banjanin, David Root, Tim H. Bruemmendorf, Juliette E. Pearce, Andreas Schuppert, Eric M. J. Bindels, Marieke A. Essers, Dirk Heckl, Thomas Stiehl, Ivan G. Costa, Benjamin L. Ebert, Rebekka K. Schneider
Summary: Genetic haploinsufficiency plays a crucial role in the clonal dominance of 5q-HSCs, providing a unique competitive advantage through increased HSC self-renewal and proliferation capacity, as well as increased fitness under inflammatory stress.
Article
Hematology
Guntram Buesche, Huesniye Teoman, Rebekka K. Schneider, Flavia Ribezzo, Benjamin L. Ebert, Aristoteles Giagounidis, Gudrun Goehring, Brigitte Schlegelberger, Oliver Bock, Arnold Ganser, Carlo Aul, Ulrich Germing, Hans Kreipe
Summary: RBC-TD anemia associated with RPS14 haploinsufficiency is a complication of MDS.del(5q), characterized by the loss of erythroblastic islands (Ery-Is) due to alterations in marrow macrophages. This loss plays a significant role in the progression of anemia.
BRITISH JOURNAL OF HAEMATOLOGY
(2022)
Article
Developmental Biology
Jitske Jansen, Bartholomeus T. van den Berge, Martijn van den Broek, Rutger J. Maas, Deniz Daviran, Brigith Willemsen, Rona Roverts, Marit van der Kruit, Christoph Kuppe, Katharina C. Reimer, Gianluca Di Giovanni, Fieke Mooren, Quincy Nlandu, Helmer Mudde, Roy Wetzels, Dirk den Braanker, Naomi Parr, James S. Nagai, Vedran Drenic, Ivan G. Costa, Eric Steenbergen, Tom Nijenhuis, Henry Dijkman, Nicole Endlich, Nicole C. A. J. van de Kar, Rebekka K. Schneider, Jack F. M. Wetzels, Anat Akiva, Johan van der Vlag, Rafael Kramann, Michiel F. Schreuder, Bart Smeets
Summary: Using organoid podocytes as a model, this study investigated idiopathic nephrotic syndrome and found that repaired organoids exhibited improved functionality, providing a valuable tool for disease modeling and therapy development.
Article
Hematology
Ursula S. A. Stalmann, Bella Banjanin, Inge A. M. Snoeren, James S. Nagai, Nils B. Leimkuehler, Ronghui Li, Adam Benabid, Jessica E. Pritchard, Hanna Malyaran, Sabine Neuss, Eric M. Bindels, Ivan G. Costa, Rebekka K. Schneider
Summary: Among bone marrow stromal cells, mesenchymal stromal cells (MSCs) are of particular interest due to their support for hematopoiesis, contribution to disease progression, therapy resistance, and leukemic initiation. In this study, cultured bone marrow-derived stromal cells (cBMSCs) were compared with in vivo counterparts on a transcriptome level. The results showed that cultured BMSCs are a homogeneous cell population, independent of isolation method, and more similar to in vivo fibroblast populations. Furthermore, TGFb inhibition in vitro can preserve a more MSC-like phenotype.
EXPERIMENTAL HEMATOLOGY
(2022)
Article
Genetics & Heredity
Yaoxian Xu, Christoph Kuppe, Javier Perales-Paton, Sikander Hayat, Jennifer Kranz, Ali T. Abdallah, James Nagai, Zhijian Li, Fabian Peisker, Turgay Saritas, Maurice Halder, Sylvia Menzel, Konrad Hoeft, Annegien Kenter, Hyojin Kim, Claudia R. C. van Roeyen, Michael Lehrke, Julia Moellmann, Thimoteus Speer, Eva M. Buhl, Remco Hoogenboezem, Peter Boor, Jitske Jansen, Cordula Knopp, Ingo Kurth, Bart Smeets, Eric Bindels, Marlies E. J. Reinders, Carla Baan, Joost Gribnau, Ewout J. Hoorn, Joachim Steffens, Tobias B. Huber, Ivan Costa, Jurgen Floege, Rebekka K. Schneider, Julio Saez-Rodriguez, Benjamin S. Freedman, Rafael Kramann
Summary: Adult kidney organoids, known as tubuloids, are derived from a distinct CD24(+) epithelial subpopulation and can be used to model autosomal dominant polycystic kidney disease (ADPKD) and study drug response. The study found similarities in gene expression between gene-edited tubuloids and ADPKD patients' tissue, demonstrating the potential of tubuloids in ADPKD disease modeling. Additionally, the approved drug for ADPKD, tolvaptan, was found to have a significant effect on cyst size in tubuloids but not in pluripotent stem cell-derived models.
Article
Multidisciplinary Sciences
Christian Niederau, Shruti Bhargava, Rebekka Schneider-Kramman, Joachim Jankowski, Rogerio B. Craveiro, Michael Wolf
Summary: Xanthohumol (XN) has been found to have a selective anti-inflammatory effect in cementoblasts, making it a potential candidate for inflammation modulation in orthodontic therapy and treatment of periodontitis.
SCIENTIFIC REPORTS
(2022)
Article
Hematology
Ruth-Miriam Koerber, Rebekka K. Schneider, Jessica Ellen Pritchard, Lino L. Teichmann, Udo Schumacher, Peter Brossart, Ines Guetgemann
Summary: In this study, we investigated the cellular expression of nestin in bone marrow trephine biopsies of MPN patients. The results showed that nestin expression following myeloablation is inversely correlated with cellularity in the bone marrow. Nestin is highly expressed in mature osteocytes, arteriolar endothelial and perivascular cells, and small capillaries within the bone marrow space.
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Review
Oncology
John Mascarenhas, Helene F. E. Gleitz, Helen T. Chifotides, Claire N. Harrison, Srdan Verstovsek, Alessandro Maria Vannucchi, Raajit K. Rampal, Jean-Jacques Kiladjian, William Vainchenker, Ronald Hoffman, Rebekka K. Schneider, Alan F. List
Summary: Myelofibrosis is a myeloproliferative disorder with significant biological and clinical heterogeneity. It can manifest as a cytopenic phenotype or a myeloproliferative phenotype, each with different prognosis and response to treatment. Patients with the cytopenic phenotype have worse outcomes and pose challenges in treatment. Recent data suggest that an innate immune deregulated state may be associated with the cytopenic phenotype, providing potential for novel treatment approaches.
Article
Immunology
Felix Schreibing, Monica T. Hannani, Hyojin Kim, James S. Nagai, Fabio Ticconi, Eleanor Fewings, Tore Bleckwehl, Matthias Begemann, Natalia Torow, Christoph Kuppe, Ingo Kurth, Jennifer Kranz, Dario Frank, Teresa M. Anslinger, Patrick Ziegler, Thomas Kraus, Juergen Enczmann, Vera Balz, Frank Windhofer, Paul Balfanz, Christian Kurts, Gernot Marx, Nikolaus Marx, Michael Dreher, Rebekka K. Schneider, Julio Saez-Rodriguez, Ivan Costa, Sikander Hayat, Rafael Kramann
Summary: We investigated the CD8(+) T cell response to SARS-CoV-2 infection and found enhanced CD8(+) T cell exhaustion in severe cases. We identified a population of NK-like, terminally differentiated CD8(+) effector T cells characterized by the expression of FCGR3A (encoding CD16). Further analysis revealed heterogeneity among CD16(+) NK-like CD8(+) T cells and significant differences in cytotoxicity, exhaustion, and NK-like differentiation between mild and severe cases.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Dermatology
Ahmed E. I. Hamouda, Carmen Schalla, Antonio Sechi, Martin Zenke, Rebekka K. Schneider, Thomas Hieronymus
Summary: This study investigates the importance of HGF receptor/Met signaling in the migration of skin-resident Langerhans cells (LCs) and dermal dendritic cells (DCs) towards draining lymph nodes. It demonstrates that Met deficiency impairs podosome formation and gelatin degradation in DCs, leading to inefficient crossing of the basement membrane. HGF-dependent Met activation reduces adhesion and enhances motility of DCs in collagen matrices, while Met signaling has no impact on integrin-independent amoeboid migration.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
Article
Biochemical Research Methods
Veronica Miguel, Katharina Charlotte Reimer, Anna Katharina Galyga, Jitske Jansen, Julia Moellmann, Luisa Meyer, Rebekka K. Schneider, Rafael Kramann
Summary: Metabolic derangement plays a crucial role in kidney pathophysiology, and organoids have shown great promise as an in vitro tool for kidney research. In this study, we present a refined protocol for analyzing bioenergetics in single kidney organoids derived from human induced pluripotent stem cells (iPSCs) using Seahorse XF96. Our protocol includes generating self-organized three-dimensional kidney organoids and preparing them for Seahorse XF96 analysis, followed by stress tests to determine mitochondrial and glycolytic rates in the organoids.
Article
Genetics & Heredity
Adam Benabid, Rebekka K. Schneider
Summary: Single-cell, multi-omic characterization reveals the role of inflammation in the transformation from myeloproliferative neoplasm to secondary acute myeloid leukemia, affecting both mutant clones and non-mutant counterparts.
Article
Biochemical Research Methods
Katharina C. Reimer, Jitske Jansen, Gijs J. Overheul, Pascal Miesen, Ronald P. van Rij, Sergio H. Triana, Bart Smeets, Rebekka K. Schneider, Rafael Kramann
Summary: We present a protocol for single-cell RNA sequencing of SARS-CoV-2-infected human induced pluripotent stem cell (iPSC)-derived kidney organoids. The protocol involves obtaining single-cell suspensions through mechanical and enzymatic disruption of the organoids, followed by processing the cells into sequencing-ready SARS-CoV-2-targeted libraries. Sequencing analysis reveals alterations in kidney cells post virus infection.
