Article
Biochemistry & Molecular Biology
Hao Cui, Rui Xu, Yu Li, Yaqiong Guo, Ziding Zhang, Lihua Xiao, Yaoyu Feng, Na Li
Summary: This study suggests that INS-16 may play important roles in the development of C. parvum and could be a valid target for the development of effective treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Justyna Jaskiewicz, Denise Ann E. D. Dayao, Donald Girouard, Derin Sevenler, Giovanni Widmer, Mehmet Toner, Saul Tzipori, Rebecca Sandlin
Summary: Cryptosporidium hominis is a major cause of childhood diarrhea in developing countries, and the lack of cryopreservation and simple culturing methods hinders the development of therapeutics. This study successfully preserved Cryptosporidium hominis TU502 oocysts by vitrification, allowing for wider access to biological specimens for research purposes. The successful cryopreservation method is significant for the scientific and clinical research of this parasite.
Article
Multidisciplinary Sciences
Naimat Ullah Khan, Tahir Usman, Mian Saeed Sarwar, Hazrat Ali, Ali Gohar, Muhammad Asif, Fazli Rabbani, Rifat Ullah Khan, Nighat Sultana, Nazir Ahmad Khan, Muhammad Mobashar, Assar Ali Shah, Metha Wanapat
Summary: The study investigated the prevalence and risk factors of Cryptosporidium infection in diarrheic sheep in Pakistan using simple microscopy and PCR techniques. Results showed age and sex were important factors affecting prevalence, with molecular detection being more efficient and sensitive compared to simple microscopy.
Article
Multidisciplinary Sciences
Naimat Ullah Khan, Tahir Usman, Mian Saeed Sarwar, Hazrat Ali, Ali Gohar, Muhammad Asif, Fazli Rabbani, Rifat Ullah Khan, Nighat Sultana, Nazir Ahmad Khan, Muhammad Mobashar, Assar Ali Shah, Metha Wanapat
Summary: This study investigated the prevalence and risk factors of Cryptosporidium infection in diarrheic sheep in Pakistan, comparing the sensitivity of two diagnostic techniques. The research concluded that molecular detection through PCR is more efficient, specific, and sensitive than simple microscopy for detecting Cryptosporidium infection.
Article
Chemistry, Multidisciplinary
Roozbeh Siavash Moakhar, Rohan Mahimkar, Arash Khorrami Jahromi, Sahar Sadat Mahshid, Carolina del Real Mata, Yao Lu, Fabio Vasquez Camargo, Brent Dixon, John Gilleard, Alexandre J. Da Silva, Momar Ndao, Sara Mahshid
Summary: Cryptosporidium parvum is a waterborne parasitic pathogen with highly infectious oocysts. Traditional detection methods are slow and labor-intensive. Therefore, the development of new sensing platforms for rapid and accurate identification is crucial.
Article
Biotechnology & Applied Microbiology
Elisa A. Mayerberger, Sajedehalsadat Yazdanparast Tafti, Sabrina S. Jedlicka, Kristen L. Jellison
Summary: Polydopamine surfaces functionalized with glycosaminoglycans facilitate the attachment and excystation of Cryptosporidium parvum oocysts. A surface-expressed receptor on the oocyst wall plays a key role in excystation, with glycosaminoglycans serving as ligands that trigger the initiation of the process.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Parasitology
Dong-Fang Li, Zhao-Hui Cui, Lu-Yang Wang, Kai-Hui Zhang, Le-Tian Cao, Shuang-Jian Zheng, Long-Xian Zhang
Summary: This study identified proteins involved in the excystation of Cryptosporidium andersoni oocysts using proteomic analysis. A total of 17 differentially expressed proteins were found, with functions related to gene expression, transcription, biosynthesis, and metabolism. The data may contribute to the identification of genes for diagnosis, vaccine development, and immunotherapy for Cryptosporidium.
PARASITES & VECTORS
(2021)
Article
Biotechnology & Applied Microbiology
Alyaa Farid, Silvia Aiad, Gehan Safwat
Summary: Cryptosporidiosis is an intestinal infection caused by the protozoan parasite Cryptosporidium spp. This study aimed to produce antibodies for the diagnosis of cryptosporidiosis and investigate their protective effects in infected calves and mice. The results showed that the produced antibodies had high sensitivity in detecting the disease and significantly reduced oocyst shedding and inflammatory cytokine production.
Article
Veterinary Sciences
Jeong-Byoung Chae, Seung-Uk Shin, Serim Kim, Young-Mi Jo, Hyunsoo Roh, Hansong Chae, Won-Gyeong Kim, Joon-Seok Chae, Hyuk Song, Jung-Won Kang
Summary: This study aimed to investigate the prevalence and molecular traits of CSpV1 in diarrheal feces of Hanwoo calves in Korea. PCR analysis revealed a higher infection rate of CSpV1 in C. parvum-positive samples, and the CSpV1 samples detected in the same farm clustered together. This is the first report on the prevalence and molecular characteristics of CSpV1 in Hanwoo calves in Korea, providing important insights into the relationship between C. parvum and CSpV1 in bovine hosts.
VETERINARY SCIENCES
(2023)
Article
Microbiology
Rui Xu, Cong Lai, Fuxian Yang, Qiang Zhang, Na Li, Yaqiong Guo, Lihua Xiao, Yaoyu Feng
Summary: This study identified that INS-21 and INS-23 of Cryptosporidium parvum are expressed in different organelles and may have distinct functions in the development of the parasite.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Parasitology
Peng Lai, Xin Yang, Yun-Hui Li, Yan-Ling Yin, Qian Yao, Shuang Huang, Ying-Ying Fan, Jun-Ke Song, Guang-Hui Zhao
Summary: Cryptosporidium parvum is a significant parasite that causes severe diarrhea in both humans and animals. This study suggests that CpCaM may be involved in the growth of C. parvum.
PARASITOLOGY RESEARCH
(2023)
Article
Microbiology
Amandine Guerin, Nathan H. Roy, Emily M. Kugler, Laurence Berry, Janis K. Burkhardt, Jung-Bum Shin, Boris Striepen
Summary: This study reveals the molecular mechanisms of Cryptosporidium invading host cells, identifying multiple parasite proteins injected into the host cell and a unique mechanism. One identified effector, ROP1, accumulates in the host cell's terminal web through direct interaction with the host protein LMO7, impacting parasite burden in vivo when genetically ablated.
CELL HOST & MICROBE
(2021)
Letter
Parasitology
Yuqing Wang, Heng Zhao, Yanan Zhang, Lei Yan
Summary: The study by Yin et al. on Cryptosporidium infection-induced aberrant expression of circular RNA profiles in HCT-8 cells is of great interest. The authors recommend careful selection of statistical methods to avoid false positives in microarray analysis, and provide suggestions for more appropriate methods in the article. Accurate analysis results are crucial for providing a convincing basis for subsequent experiments.
PARASITES & VECTORS
(2021)
Article
Immunology
Ruchika Shakya, Alejandro Jimenez Melendez, Lucy J. Robertson, Mette Myrmel
Summary: Neonatal diarrhea in calves is a major health problem in the cattle industry. This study investigated the influence of co-infections of Cryptosporidium parvum and bovine coronavirus (BCoV) in human HCT-8 cells. The results showed that prior inoculation with either pathogen had no influence on the other, but simultaneous co-inoculation increased the entry of viral particles when C. parvum sporozoites were present. Flow cytometry and confocal microscopy results indicated different infection patterns for the two pathogens in HCT-8 cells.
MICROBES AND INFECTION
(2022)
Article
Green & Sustainable Science & Technology
Inhyeong Jeon, Eric C. Ryberg, Pedro J. J. Alvarez, Jae-Hong Kim
Summary: Poor access to safe drinking water is a major global sustainability issue. Solar disinfection provides a feasible solution. Here the authors examine the potential of five most typical types of this technology, revealing their unique challenges and opportunities.
NATURE SUSTAINABILITY
(2022)