Article
Multidisciplinary Sciences
Dina M. Metwally, Reem Alajmi, Muslimah N. Alsulami, Isra M. Al-Turaiki, Rewaida Abdel-Gaber, Afrah F. Alkhuriji, Haleema H. Albohiri, Khalil Mohamed, Hanadi B. Baghdadi, Manal F. El-Khadragy, Guillermo T. Isaias, Saeed El-Ashram
Summary: The study found that Theileria spp. were detected in 57.8% of sheep and 51.9% of goats in Jeddah, Saudi Arabia. Phylogenetic analysis of the sequence data revealed that the T. ovis identified in this study were closely related to an isolate from Turkey, with pairwise identity ranging from 84.4% to 99.8% and coverage ranging from 52.35% to 99.79%.
Article
Multidisciplinary Sciences
Shakeel Hussain, Syed Mohsin Bukhari, Lixin Wang, Nimra Khalid, Zhijun Hou
Summary: The study found Cryptosporidium spp. in captive zoo felids in North-Eastern China with an overall prevalence of 9.43%. Among the isolates, 6 were identified as Cryptosporidium parvum while the rest resembled Cryptosporidium NEV10. This research suggests the importance of considering control policies for zoonotic diseases in zoo settings.
Article
Microbiology
Rauni Kivisto, Sofia Kamarainen, Otso Huitu, Jukka Niemimaa, Heikki Henttonen
Summary: This study reveals the presence of Cryptosporidium protozoa in mouse, vole, and shrew populations around Finland, with the highest zoonotic risk associated with C. ditrichi in Apodemus flavicollis and C. andersoni in Craseomys rufocanus. The most common zoonotic species in human infections, C. parvum, was not detected in the study.
Article
Immunology
Jose L. Huaman, Carlo Pacioni, Lily Kenchington-Evans, Mark Doyle, Karla J. Helbig, Teresa G. Carvalho
Summary: Wild deer populations in Australia have grown significantly, posing a biosecurity risk to the livestock industry. This study investigated the presence of the intestinal parasite Entamoeba bovis in wild deer and domestic cattle in south-eastern Australia. The study found high prevalence of E. bovis in both cattle and wild deer, and identified new variants of Entamoeba in different deer species. Cross-species transmission of E. bovis between wild deer and cattle has not yet occurred in Australia. Continued surveillance and epidemiological investigations are needed to understand the emergence and transmission of Entamoeba parasites to livestock.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Ahmet Efe Koseoglu, Huseyin Can, Muhammet Karakavuk, Mervenur Guvendi, Aysu Degirmenci Doskaya, Pumla Bhekiwe Manyatsi, Mert Doskaya, Adnan Yuksel Guruz, Cemal Un
Summary: This study investigated the molecular prevalence of Cryptosporidium spp. in stray cats in Turkey. It found that a small percentage of the fecal samples were positive for Cryptosporidium felis, and these samples belonged to the XIXa subtype family, which is closely related to human subtypes of the parasite.
BMC VETERINARY RESEARCH
(2022)
Article
Parasitology
Sandor Hornok, Sandor A. Boldogh, Nora Takacs, Jeno Kontschan, Sandor Szekeres, Endre Sos, Attila D. Sandor, Yuanzhi Wang, Barbara Tuska-Szalay
Summary: This study reports the occurrence of tick-borne protozoan parasites in ticks and tissues of wild cats, mustelids, and red squirrels in Hungary. Several new genotypes of Hepatozoon and Cytauxzoon species were identified, including a new genotype of Hepatozoon fells in wild cats. New tick-host associations were also observed. The study suggests that H. fells and H. mortis can survive transstadially in different tick species.
PARASITES & VECTORS
(2022)
Article
Immunology
Gael Even, Ana Lokmer, Jules Rodrigues, Christophe Audebert, Eric Viscogliosi, Laure Segurel, Magali Chabe
Summary: The gut microbial communities of humans are influenced by the presence of gut protozoa such as Blastocystis and Entamoeba, showing associations with diversity and composition of bacterial microbiota in the gut. Different gut protozoa have distinct impacts on bacterial microbiome diversity, with Blastocystis associated with both richness and evenness, while Entamoeba associated only with richness. Further experimental studies are needed to explore the specific mechanisms of interaction between these protozoa and gut bacteria.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Food Science & Technology
Niki Mougiou, Antiopi Tsoureki, Spyros Didos, Ioanna Bouzouka, Sofia Michailidou, Anagnostis Argiriou
Summary: The analysis of table olives microbiome using next-generation sequencing has provided valuable information about the microbial community composition of this popular fermented food. This study used 16S and 18S rRNA sequencing to evaluate the microbial communities of table olives from five Greek popular cultivars, fermented by Greek or Spanish style. The results showed that different curing and fermentation methods had an impact on the microbial composition and biochemical properties of the olives.
Article
Veterinary Sciences
Anil Kumar Nehra, Ansu Kumari, Aman Dev Moudgil, Sukhdeep Vohra
Summary: This study investigated the genetic diversity of T. annulata and identified other Theileria spp. sequences through analysis of 18S rRNA gene sequences in GenBank. It was found that some sequences previously identified as T. annulata were misidentified, and a high level of sequence conservation was observed in the 18S rRNA gene of T. annulata and T. orientalis sequences.
BMC VETERINARY RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Chenghao Jia, Yue Zhang, Qiang Xu, Chunyang Sun, Yanan Wang, Fei Gao
Summary: This study investigated the eukaryotic food sources of three tropical sea cucumber species and compared their differences. The results showed that the feeding preference differed among the species, possibly due to differences in tentacle morphology and habitat preferences. The sea cucumber gut enriched certain microorganisms and exhibited selective feeding for sediment patches.
Article
Parasitology
Sandor Hornok, Andrei D. Mihalca, Jeno Kontschan, Nora Takacs, Denis Fedorov, Olivier Plantard, Attila D. Sandor
Summary: This study provides high-resolution digital pictures and molecular data of male and female I. rugicollis for the first time, and morphological comparison with I. cornutus could not resolve uncertainties in the latter species' validity. Further molecular comparisons with new specimens of I. cornutus are needed to confirm its status. This study represents the first comprehensive molecular phylogenetic analysis of western Palearctic Pholeoixodes species, including I. rugicollis, and confirms the phylogenetic position of the subgenus Eschatocephalus within Pholeoixodes.
PARASITES & VECTORS
(2023)
Article
Biochemistry & Molecular Biology
Daniel Vaulot, Clarence Wei Hung Sim, Denise Ong, Bryan Teo, Charlie Biwer, Mahwash Jamy, Adriana Lopes dos Santos
Summary: Metabarcoding has become a popular method for studying microbial eukaryotic communities, with a newly-assembled database metaPR(2) providing processed 18S rRNA metabarcodes annotated with the PR2 reference sequence database. This database, containing over 4000 samples and 90,000 ASVs, is accessible through web-based interface and an R package, offering valuable resources for researchers studying protist diversity in various systems.
MOLECULAR ECOLOGY RESOURCES
(2022)
Article
Immunology
Vikrant Sudan, Daya Shanker, Sanjhi Paliwal, Rahul Kumar, Amit Singh
Summary: Sarcocystis fusiformis is the most prevalent species in buffaloes globally, with Indian isolates showing high nucleotide homologies in both 18S rRNA and cox 1 genes, suggesting the cox 1 gene is better for delineating evolutionary phylogenetics.
MICROBIAL PATHOGENESIS
(2021)
Article
Environmental Sciences
Thuy Tram Nguyen, Thi Trang Le, Huy Hoang Le, Thi Nga Tang, Thi Thu Hang Phung, Thi Mai Hien Vu, Thi Hai Au Tran, Dong Tu Nguyen, Thi Phuong Lien Nguyen, Duc Phuc Pham, Panagiotis Karanis
Summary: This study aimed to investigate the presence of Cryptosporidium and Giardia in environmental samples and fecal samples of biogas users in Bac Giang province, Vietnam. The results showed that a small percentage of samples tested positive for Cryptosporidium oocysts and Giardia cysts. The presence of certain genotypes and assemblages of these protozoa were identified, including some that were previously unknown in humans in Bac Giang.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Blaire M. Padayhag, Michael Angelou L. Nada, Jake Ivan P. Baquiran, Marilou P. Sison-Mangus, Maria Lourdes San Diego-McGlone, Patrick C. Cabaitan, Cecilia Conaco
Summary: This study found that the microbial biofilm communities near mariculture areas can affect the settlement of coral larvae. The biofilm farthest from the mariculture area had a higher effectiveness in inducing the settlement of Acropora tenuis larvae. These biofilms were characterized by a greater proportion of crustose coralline algae and gammaproteobacterial taxa, while biofilms closer to the mariculture zone had a higher proportion of cyanobacteria and no crustose coralline algae. These findings suggest that nutrient enrichment from mariculture activities can alter the composition of biofilm-associated microbiome and indirectly affect coral larval settlement.
MARINE POLLUTION BULLETIN
(2023)
Article
Parasitology
Yvonne Qvarnstrom, Theresa Benedict, Paula L. Marcet, Ryan E. Wiegand, Barbara L. Herwaldt, Alexandre J. da Silva
Article
Biotechnology & Applied Microbiology
Helen R. Murphy, Hediye Nese Cinar, Gopal Gopinath, Kathy E. Noe, Lacresha D. Chatman, Nancy E. Miranda, June H. Wetherington, Jason Neal-McKinney, Gabrielle S. Pires, Elizabeth Sachs, Kristopher J. Stanya, Cynthia L. Johnson, Fernanda S. Nascimento, Monica Santin, Aleksey Molokin, Mansour Samadpour, Harish Janagama, Amy Kahler, Candace Miller, Alexandre J. da Silva
Article
Multidisciplinary Sciences
Carolina Valenca Barbosa, Magali Muniz Barreto, Rosemary de Jesus Andrade, Fernando Sodre, Claudia Masini d'Avila-Levy, Jose Mauro Peralta, Ricardo Pereira Igreja, Heloisa Werneck de Macedo, Helena Lucia Carneiro Santos
Article
Multidisciplinary Sciences
Carolina Valenca-Barbosa, Teresa Cristina Bergamo do Bomfim, Bernardo Rodrigues Teixeira, Rosana Gentile, Socrates Fraga da Costa Neto, Barbara Souza Neil Magalhaes, Daniel de Almeida Balthazar, Fabio Alves da Silva, Renata Biot, Claudia Masini d'Avila Levy, Helena Lucia Carneiro Santos
Article
Pathology
Rita C. P. Rampazzo, Ana Claudia Graziani, Keren K. Leite, Jhully Anni Surdi, Cheysa A. Biondo, Maykon L. N. Costa, Thiago Jacomasso, Marco Cereda, Marco De Fazio, Marco A. Bianchessi, Otacilio C. Moreira, Constanca Britto, Joana D. N. Costa, Viviane M. Goes, Alexandre J. da Silva, Marco A. Krieger, Alexandre D. T. Costa
JOURNAL OF MOLECULAR DIAGNOSTICS
(2019)
Article
Public, Environmental & Occupational Health
Marcos de Almeida, Fernanda S. Nascimento, Blaine A. Mathison, Henry Bishop, Richard S. Bradbury, Vitaliano A. Cama, Alexandre J. da Silva
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2020)
Article
Veterinary Sciences
Alexandre J. da Silva, Alessandra L. Morassutti
Summary: This review highlights the importance of human angiostrongyliasis as a significant foodborne zoonosis caused by Angiostrongylus costaricensis and Angiostrongylus cantonensis. The re-emergence and urban expansion of angiostrongyliasis pose significant concerns for the control of these infections worldwide. The article focuses on various aspects of the disease, with a special emphasis on the illness caused by A. cantonensis.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Agronomy
Lidiane Tavares Duarte Berriel, Adriana Pittella Sudre, Helena Lucia Carneiro Santos, Teresa Cristina Bergamo do Bomfim
Summary: This study investigated the presence of Cryptosporidium spp. in domestic ducks and found that 10 out of 315 samples collected from the ducks were positive for Cryptosporidium baileyi, a species closely related to gastric species and of importance in animal health.
Article
Microbiology
Mauricio Durigan, Emma Patregnani, Gopal R. Gopinath, Laura Ewing-Peeples, Chaeyoon Lee, Helen R. Murphy, Sonia Almeria, Hediye Nese Cinar, Flavia Negrete, Alexandre J. da Silva
Summary: This study developed a new sequencing-based method for detecting the coccidian parasite Cyclospora cayetanensis, which causes diarrheal illness outbreaks. The method showed high specificity and was able to accurately detect C. cayetanensis in food and water samples. This sensitive detection method adds to the range of screening strategies for food and water samples.
Article
Multidisciplinary Sciences
Hediye Nese Cinar, Gopal Gopinath, Sonia Almeria, Joyce M. Njoroge, Helen R. Murphy, Alexandre da Silva
Summary: This study developed a highly sensitive custom-designed targeted sequencing method capable of obtaining near-complete mitochondrial genome sequences of C. cayetanensis from food samples with low levels of contamination. The success of this method represents a critical milestone towards the potential development of a comprehensive genotyping method for C. cayetanensis.
Article
Parasitology
Amy M. Kahler, Mia C. Mattioli, Alexandre J. da Silva, Vincent Hill
Summary: This study evaluated three sample collection methods for recovery of C. cayetanensis oocysts from water samples, with DEUF and CFC being suitable for high turbidity and organic matter content water sources, respectively.
FOOD AND WATERBORNE PARASITOLOGY
(2021)
Article
Parasitology
Alexandre D. T. Costa, Thiago Jacomasso, Elaine C. Mattos, Aline B. Farias, Rita C. P. Rampazzo, Rebeka S. Pinto, Walleyd Tassi, Maria Aparecida M. Marciano, Vera Lucia Pereira-Chioccola, Helen R. Murphy, Alexandre J. da Silva, Marco A. Krieger
Summary: This study introduces a new gelified reaction format for qPCR reagents, which improves stability, reduces operator errors, and allows for storage at lower temperatures for up to 90 days. Results suggest that this gelification method may help achieve more reproducible qPCR data across laboratories, supporting surveillance actions.
FOOD AND WATERBORNE PARASITOLOGY
(2021)
Article
Biodiversity Conservation
Priscilla da Silva Pereira, Natalia Freitas de Souza, Darcilio Fernandes Baptista, Marcelo Ribeiro-Alves, Helena Lucia Carneiro Santos, Daniel Forsin Buss
Summary: This study used benthic macroinvertebrates Functional Feeding Group (FFG) as indicators to assess the effects of stream variables on FFG abundance and richness, and evaluate FFG ratios as a tool to assess ecological conditions. Results showed significant differences in FFG richness among sites with different impairment conditions, and FFGs responded differently to abiotic variables. The FFG ratio analysis was found to be a fast and cost-effective tool for monitoring aquatic ecosystems in the Atlantic Forest biome, but further calibration is needed for this region.
Article
Infectious Diseases
Claudia M. d'Avila-Levy, Bertrand Bearzatto, Jerome Ambroise, Raphael Helaers, Anzhelika Butenko, Vyacheslav Yurchenko, Karina A. Morelli, Helena L. C. Santos, Pascal Brouillard, Philippe Grellier, Jean-Luc Gala, Miikka Vikkula
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2020)
Article
Parasitology
Carolina Boucinha, Amanda R. Caetano, Helena L. C. Santos, Raphael Healers, Miikka Vikkula, Marta Helena Branquinha, Andre Luis Souza dos Santos, Philippe Grellier, Karina Alessandra Morelli, Claudia M. d'Avila-Levy
MEMORIAS DO INSTITUTO OSWALDO CRUZ
(2020)