Article
Biochemistry & Molecular Biology
Gagan Deep Jhingan, Maria Manich, Jean-Christophe Olivo-Marin, Nancy Guillen
Summary: Entamoeba histolytica is a protozoan parasite that causes amoebiasis in humans. It utilizes its actin-rich cytoskeleton to invade human tissues, kill and phagocytose human cells. The parasite has developed sophisticated systems to integrate internal and external signals, coordinate cell shape changes, and facilitate motility. Phosphorylation events and related signalling mechanisms are important in this process, as inhibition of phosphatidylinositol 3-kinases leads to changes in motility, morphology, and actin-rich adhesive structures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Ruofan Peng, Shigeo Yoshinari, Tetsuro Kawano-Sugaya, Ghulam Jeelani, Tomoyoshi Nozaki
Summary: HSPC117/RtcB, a 3'-phosphate tRNA ligase, is involved in tRNA splicing and maturation, as well as mRNA splicing of certain protein-coding genes. In Entamoeba histolytica, EhRtcB1 is nuclear-localized while EhRtcB2 is cytosolic, with EhRtcB1 playing a predominant role in tRNA processing. Both EhRtcB1 and EhRtcB2 exhibit RNA ligation activity in vitro. The study demonstrates conservation of tRNA splicing and functional diversification of RtcBs in this lineage.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yana Shaulov, Lotem Sarid, Meirav Trebicz-Geffen, Serge Ankri
Summary: The study demonstrates that Auranofin-adapted Entamoeba histolytica trophozoites exhibit impaired growth, increased sensitivity to oxidative and nitrosative stress, and reduced levels of oxidative products compared to acute AF-exposed trophozoites. Additionally, overexpression of the mammalian thioredoxin reductase does not protect the parasite against AF, indicating that it is not central to the adaptation mechanism.
Article
Immunology
Xia Li, Meng Feng, Yanqing Zhao, Yuhan Zhang, Ruixue Zhou, Hang Zhou, Zhen Pang, Hiroshi Tachibana, Xunjia Cheng
Summary: Macrophages play a crucial role in combating amoebiasis by releasing pro-inflammatory cytokines in response to infection. Entamoeba histolytica can activate the NLRP3 inflammasome through secretion of peroxiredoxins during interaction with host cells, potentially offering a novel therapeutic approach for this global threat.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Microbiology
Yasuaki Yanagawa, Shinji Izumiyama, Yumiko Saito-Nakano, Kumiko Nakada-Tsukui, Seiki Kobayashi, Naoko Yoshida, Yoshimi Kikuchi, Hiroyuki Gatanaga, Shinichi Oka, Tomoyoshi Nozaki, Koji Watanabe
Summary: The severity of Entamoeba histolytica infection is determined by host immunology, pathogen virulence, and the intestinal environment. Conventional research for assessing pathogen virulence has been mainly performed using laboratory strains, and it is unclear whether this knowledge can be applied to the true pathogenesis. Transcriptomic analysis of clinical and laboratory strains revealed significant differences in gene expression, indicating that the changes caused by liver challenge may not be consistent between clinical and laboratory strains. Environmental stress can also enhance the virulence of the laboratory strain through a shift in gene expression profile.
Review
Biochemistry & Molecular Biology
Sudha Bhattacharya
Summary: Entamoeba histolytica, the causative agent of amoebiasis, initiates DNA replication from multiple sites on episomal circles and uses different origins under various growth conditions. Biochemical studies have characterized replication and recombination proteins in this parasite, providing a valuable resource for the exploration of novel anti-amoebic drug targets.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Infectious Diseases
Sanjib Sardar, Ajanta Ghosal, Tapas Haldar, Maimoon Maruf, Koushik Das, Yumiko Saito-Nakano, Seiki Kobayashi, Shanta Dutta, Tomoyoshi Nozaki, Sandipan Ganguly
Summary: This study determined the prevalence and characterized the E. moshkovskii isolates in eastern India. The results showed a high prevalence rate of E. moshkovskii infection with a unique seasonal pattern. Additionally, certain SNPs in the 18S rRNA gene were associated with clinical features, indicating their potential as markers for identifying pathogenic isolates of E. moshkovskii. These findings highlight the epidemiological significance of E. moshkovskii as a possible emerging enteric pathogen in India.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Review
Microbiology
Ascel Samba-Louaka, Elisabeth Labruyere, Mariette Matondo, Marie Locard-Paulet, Jean-Christophe Olivo-Marin, Nancy Guillen
Summary: Amoebae, including pathogenic species and free-living species, are found in both aquatic and terrestrial environments and pose significant threats to public health. They exist in two cellular forms, trophozoites and cysts, with the cyst stage being the resistant and disseminating form. Encystation is accompanied by stress responses and limited data suggest that it shares factors and mechanisms with stress responses in trophozoites exposed to toxic compounds. Understanding the molecular mechanisms underlying amoebae-specific stress responses is crucial for the development of effective therapies.
Article
Immunology
Shijiang Mi, Lihua Wang, Hongwei Li, Fei Bao, Rachel Madera, Xiju Shi, Liying Zhang, Yingying Mao, Renhe Yan, Xianzhu Xia, Wenjie Gong, Jishu Shi, Changchun Tu
Summary: This study systematically characterized monoclonal antibodies against classical swine fever virus (CSFV) and identified key antigenic epitopes associated with these antibodies. The results suggest that certain mutations may arise from long-term vaccination and result in viral evasion of host immune response. This study provides new insights into the development of a diagnostic strategy to differentiate vaccine strains from field isolates.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Francisco Sierra-Lopez, Lidia Baylon-Pacheco, Sonia Cynthia Vanegas-Villa, Jose Luis Rosales-Encina
Summary: Entamoeba histolytica, an intestinal protozoan parasite, has two low molecular weight protein tyrosine phosphatase genes, EhLMW-PTP1 and EhLMW-PTP2, with one amino acid difference between them. These genes show different activities and stabilities under various conditions.
Review
Cell Biology
Lotem Sarid, Serge Ankri
Summary: This article discusses the role of host microbiota in regulating epigenetic processes in Entamoeba histolytica, as well as the implications of this knowledge for the development of new anti-amebic strategies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Microbiology
Yanqing Zhao, Xia Li, Ruixue Zhou, Lei Zhang, Lijun Chen, Hiroshi Tachibana, Meng Feng, Xunjia Cheng
Summary: In this study, the proteomic changes in host cells incubated with E. histolytica trophozoites or stimulated by purified native Igl protein were analyzed. The results showed that central carbon metabolism of host cells was suppressed in both E. histolytica-treated and Igl-treated groups, with evidence of decreased expression levels of key enzymes. Additionally, Igl had potential physical interactions with central carbon metabolism enzymes and proteasome subunit alpha and beta, suggesting a role in the degradation of key enzymes.
Article
Biochemistry & Molecular Biology
Cesar Diaz-Godinez, Joshue Fabian Jorge-Rosas, Mario Nequiz, Santiago Martinez-Calvillo, Juan P. Laclette, Carlos Rosales, Julio C. Carrero
Summary: In the interaction between Entamoeba histolytica trophozoites and neutrophils, the ROS generated by trophozoites and processed by MPO during contact are essential for inducing NETosis.
Article
Immunology
Cesar Diaz-Godinez, Diana G. Rios-Valencia, Samuel Garcia-Aguirre, Santiago Martinez-Calvillo, Julio Cesar Carrero
Summary: This study isolated and characterized extracellular vesicles (EVs) released by the human protozoan parasite Entamoeba histolytica. The EVs were found to modulate the immune response of neutrophils, reducing oxidative burst and NET release. Proteomic analysis showed differences in protein expression and abundance between EVs from amoebae and neutrophils. These findings highlight the importance of EVs in the immunomodulatory effects exerted by amoeba on neutrophils.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Yu-Ling Chang, Maura Rossetti, David W. Gjertson, Liudmilla Rubbi, Michael Thompson, Dennis J. Montoya, Marco Morselli, Felicia Ruffin, Alexander Hoffmann, Matteo Pellegrini, Vance G. Fowler Jr, Michael R. Yeaman, Elaine F. Reed
Summary: The study found that persistent methicillin-resistant Staphylococcus aureus (MRSA) bacteremia may be related to the DNA methylation profiles of patients, with different methylation signatures existing in patients experiencing antibiotic-persistent MRSA infection (APMB) compared to those with antibiotic-resolving MRSA infection (ARMB). These distinct methylation patterns, enriched in neutrophils, allowed for the prediction of APMB with an average area under the curve of 0.85, indicating a potential risk stratification strategy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
Hiroshi Tachibana, Tetsuo Yanagi, Meng Feng, K. B. Anura T. Bandara, Seiki Kobayashi, Xunjia Cheng, Kenji Hirayama, R. P. V. Jayanthe Rajapakse
JOURNAL OF EUKARYOTIC MICROBIOLOGY
(2016)
Article
Microbiology
Duo Wu, Ke Qiao, Meng Feng, Yongfeng Fu, Junlong Cai, Yihong Deng, Hiroshi Tachibana, Xunjia Cheng
JOURNAL OF EUKARYOTIC MICROBIOLOGY
(2018)
Article
Parasitology
Hiroshi Tachibana, Azumi Kakinol, Makoto Kazama, Meng Feng, Satomi Asai, Kazuo Umezawa, Tsukasa Nozaki, Takashi Makiuchil, Takayuki Kamada, Hideki Watanabe, Noriyuki Horiki, Xunjia Cheng, Gohta Masuda
Article
Parasitology
Meng Feng, Kishor Pandey, Tetsuo Yanagi, Ting Wang, Chaturong Putaporntip, Somchai Jongwutiwes, Xunjia Cheng, Jeevan B. Sherchand, Basu Dev Pandey, Hiroshi Tachibana
PARASITOLOGY RESEARCH
(2018)
Article
Infectious Diseases
Yue Guan, Meng Feng, Xiangyang Min, Hang Zhou, Yongfeng Fu, Hiroshi Tachibana, Xunjia Cheng
PLOS NEGLECTED TROPICAL DISEASES
(2018)
Article
Multidisciplinary Sciences
Makoto Kazama, Sanae Ogiwara, Takashi Makiuchi, Kazuhiro Yoshida, Kumiko Nakada-Tsukui, Tomoyoshi Nozaki, Hiroshi Tachibana
SCIENTIFIC REPORTS
(2017)
Article
Multidisciplinary Sciences
Takashi Makiuchi, Herbert J. Santos, Hiroshi Tachibana, Tomoyoshi Nozaki
SCIENTIFIC REPORTS
(2017)
Article
Microbiology
Makoto Kazama, Kazuhiro Yoshida, Sanae Ogiwara, Takashi Makiuchi, Hiroshi Tachibana
JOURNAL OF EUKARYOTIC MICROBIOLOGY
(2019)
Article
Infectious Diseases
Meng Feng, Tetsuo Yanagi, Chaturong Putaporntip, Urassaya Pattanawong, Xunjia Cheng, Somchai Jongwutiwes, Hiroshi Tachibana
INFECTION GENETICS AND EVOLUTION
(2019)
Article
Infectious Diseases
Masayuki Tanaka, Takashi Makiuchi, Tomoyoshi Komiyama, Takashi Shiina, Ken Osaki, Hiroshi Tachibana
PLOS NEGLECTED TROPICAL DISEASES
(2019)
Article
Microbiology
Meng Feng, Yuhan Zhang, Hang Zhou, Xia Li, Yongfeng Fu, Hiroshi Tachibana, Xunjia Cheng
Article
Infectious Diseases
Urassaya Pattanawong, Chaturong Putaporntip, Azumi Kakino, Naoko Yoshida, Seiki Kobayashi, Surasuk Yanmanee, Somchai Jongwutiwes, Hiroshi Tachibana
Summary: The study found that E. histolytica and/or E. dispar were prevalent among school-aged children in three rural schools in the Thai-Myanmar border region of northwest Thailand. Transmission of these Entamoeba species was suggested to occur within classrooms and at home, possibly through person-to-person contact.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Microbiology
Yanqing Zhao, Xia Li, Ruixue Zhou, Lei Zhang, Lijun Chen, Hiroshi Tachibana, Meng Feng, Xunjia Cheng
Summary: In this study, the proteomic changes in host cells incubated with E. histolytica trophozoites or stimulated by purified native Igl protein were analyzed. The results showed that central carbon metabolism of host cells was suppressed in both E. histolytica-treated and Igl-treated groups, with evidence of decreased expression levels of key enzymes. Additionally, Igl had potential physical interactions with central carbon metabolism enzymes and proteasome subunit alpha and beta, suggesting a role in the degradation of key enzymes.
Article
Biochemistry & Molecular Biology
Kentaro Kato, Hiroshi Tachibana
Summary: The protein Igl1 of Entamoeba histolytica has been found to exhibit hemolytic and cytotoxic activities. The amino acid regions 787-846, 968-1028, and 1029-1088 of Igl1 are responsible for these activities. This is the first report of a protozoan protein with hemolytic and cytotoxic activities in multiple domains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Xia Li, Yuhan Zhang, Yanqing Zhao, Ke Qiao, Meng Feng, Hang Zhou, Hiroshi Tachibana, Xunjia Cheng