Article
Infectious Diseases
Hung Manh Nguyen, Hien Hoang Van, Loan Thi Ho, Yulia V. Tatonova, Henry Madsen
Summary: The study aimed to clarify the suitability of two thiarid snail species, M. tuberculata and T. granifera, as first intermediate hosts of C. sinensis. Field surveys in Vietnam revealed that only Parafossarulus manchouricus was infected with C. sinensis, contradicting previous beliefs about the role of the other two snail species. Experimentally, only P. manchouricus was successfully infected with C. sinensis eggs.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Infectious Diseases
Men-Bao Qian, Hong-Mei Li, Zhi-Hua Jiang, Yi-Chao Yang, Ming-Fei Lu, Kang Wei, Si-Liang Wei, Yu Chen, Chang-Hai Zhou, Ying-Dan Chen, Xiao-Nong Zhou
Summary: The study in Hengxian county, China found a significant association between Clonorchis sinensis infection and hepatobiliary morbidity, with certain conditions strongly related to the infection intensity. Therefore, raising awareness on the harm of clonorchiasis is crucial for policymakers and villagers to implement effective interventions.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Veterinary Sciences
Weerachai Saijuntha, Paiboon Sithithaworn, Trevor N. Petney, Ross H. Andrews
Summary: Several foodborne zoonotic trematodes of the family Opisthorchiidae, including Clonorchis and Opisthorchis, have been reported to infect humans. At least 680 million people worldwide are at risk of infection from these liver flukes, with 45 million currently infected in Europe and Asia. These liver flukes have been proven to be causative agents of bile duct cancer, highlighting the need for comprehensive research and control programs to achieve opisthorchiasis and clonorchiasis free communities globally.
RESEARCH IN VETERINARY SCIENCE
(2021)
Review
Parasitology
Maria Y. Pakharukova, Viatcheslav A. Mordvinov
Summary: This review focuses on the significance of foodborne liver flukes and discusses the differences between O. felineus, O. viverrini, and C. sinensis. The discussion includes characteristics of their life cycles, range of intermediate hosts, genomic and transcriptomic features, and clinical symptoms and morbidity in humans.
Article
Multidisciplinary Sciences
Claudia Machicado, Maria Pia Soto, Luis Felipe La Chira, Joel Torres, Carlos Mendoza, Luis A. Marcos
Summary: The study revealed that Opisthorchis viverrini and Clonorchis sinensis have 31 and 22 secretory proteins targeting the host cell nucleus, respectively, while Fasciola hepatica does not. These proteins enrich transcription initiation and nucleic acid binding in O. viverrini and C. sinensis, and RNA processing function in F. hepatica.
Article
Parasitology
Aleksei Katokhin, Elena A. Serbina
Summary: The study of opisthorchiids larval stages associated with Bithyniidae snails is important for characterizing opisthorchiidoses foci due to the limited mobility of these host species. The overlapping foci of opisthorchiosis and metorchiosis pose difficulties in determining cercariae species, reducing the accuracy of epidemiological conclusions. This study used morphological and molecular genetic identification to confirm the associations between specific opisthorchiid species and Bithyniidae snails.
PARASITOLOGY RESEARCH
(2023)
Article
Parasitology
E. A. Serbina
Summary: Opisthorchiasis and related infections are significant public health burdens in eastern Europe to southeastern and northern Asia. This study evaluated the role of bithyniid snails in the transmission of Opisthorchiidae and Notocotylidae and found potentially dangerous opisthorchiid species in the Novosibirsk region.
PARASITOLOGY RESEARCH
(2022)
Article
Multidisciplinary Sciences
Maria Y. Pakharukova, Oxana Zaparina, Sung-Jong Hong, Banchob Sripa, Viatcheslav A. Mordvinov
Summary: The study found that liver flukes are not reservoirs of Helicobacter pylori, but infection significantly increases the prevalence and gene copy number of H. pylori while altering bile and gut microbiota by increasing the abundance of H. pylori. This may be a previously overlooked feature of liver fluke pathogenesis and the findings provide novel insights into the comparative assessment of host microbiota and Helicobacter abundance during epidemiologically important liver fluke infections.
SCIENTIFIC REPORTS
(2021)
Article
Urology & Nephrology
Pantipa Tonsawan, Sompote Intarak, Banchob Sripa, Anucha Puapairoj, Manop Sripa, Paiboon Sithithaworn, Sirirat Anutrakulchai
Summary: This study is the first to demonstrate the presence of Opisthorchis viverrini (OV) antigen in human kidney tissue, suggesting a possible association between OV infection and biopsy-proven glomerular diseases.
AMERICAN JOURNAL OF NEPHROLOGY
(2022)
Article
Microbiology
Natchita Chungkanchana, Paiboon Sithithaworn, Chanika Worasith, Phattharaphon Wongphutorn, Sirowan Ruantip, Kulthida Y. Kopolrat, Apinya Jusakul, Raynoo Thanan, Kunyarat Duenngai, Jiraporn Sithithaworn, Anchalee Techasen
Summary: This study evaluated the effects of urine concentration on the detection of parasite-specific IgG by urine enzyme-linked immunosorbent assay (ELISA). The results showed that the concentration of urine samples prior to analysis improved the sensitivity for diagnosis of strongyloidiasis. The concentrated-urine ELISA increased the proportion of positive results in individuals with borderline results or false-negative diagnosis.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Parasitology
Kulthida Y. Kopolrat, Paiboon Sithithaworn, Nadda Kiatsopit, Jutamas Namsanor, Opal Pitaksakulrat, Patiwat Yasaka, Prasert Saichua, Weerachai Saijuntha, Smarn Tesana, Ross H. Andrews, Trevor N. Petney
Summary: The study revealed that Bithynia siamensis goniomphalos serves as an intermediate host for various trematodes, with virgulate xiphidiocercariae and Opisthorchis viverrini being the most prevalent. The quantity of irrigation water for rice cultivation was found to be correlated with the transmission dynamics of trematodes in B. s. goniomphalos, impacting the peak prevalence of trematode infections in the snails. Presence of other trematodes in the snail was found to negatively impact the emergence of virgulate cercariae, highlighting the complex interactions between host and parasite in the transmission dynamics.
Article
Parasitology
Yaroslav K. Kapushchak, Oxana G. Zaparina, Viatcheslav A. Mordvinov, Maria Y. Pakharukova
Summary: Fish-borne trematode infections can cause liver diseases and may be related to kidney disorders. Using a hamster model, we found that Opisthorchis felineus infection leads to the development of glomerulopathy, characterized by changes in blood and urine markers.
Article
Fisheries
Ivan A. Kutyrev, Viatcheslav A. Mordvinov
Summary: This study analyzed the population genetic structure of D. dendriticus and D. ditremus parasitizing fish in the Baikal Rift Zone (BRZ) based on ITS1 and cox1 sequences. The study found that both species exhibited complex population genetic structures with different levels of genetic diversity. The formation of the genetic structure in these tapeworms was likely influenced by different palaeoecological conditions and lake recolonization processes.
DISEASES OF AQUATIC ORGANISMS
(2022)
Review
Parasitology
Maria Y. Pakharukova, Viatcheslav A. Mordvinov
Summary: This review focuses on the significance of foodborne liver flukes and discusses the differences between O. felineus, O. viverrini, and C. sinensis. The discussion includes characteristics of their life cycles, range of intermediate hosts, genomic and transcriptomic features, and clinical symptoms and morbidity in humans.
Article
Biology
Thomas Crellen, Melissa Haswell, Paiboon Sithithaworn, Somphou Sayasone, Peter Odermatt, Poppy H. L. Lamberton, Simon E. F. Spencer, T. Deirdre Hollingsworth
Summary: A study on opisthorchiasis found that parasite fecundity varies between populations and the relationship between routine diagnostics and true prevalence is influenced by parasite aggregation.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Article
Multidisciplinary Sciences
Chatanun Eamudomkarn, Sirowan Ruantip, Jiraporn Sithithaworn, Anchalee Techasen, Kulthida Y. Kopoolrat, Chanika Worasith, Phattharaphon Wongphutorn, Jeffrey M. Bethony, Thewarach Laha, Paiboon Sithithaworn
Summary: Detection of anti-Strongyloides IgG in urine by ELISA showed comparable performance to conventional serum assays for diagnosing strongyloidiasis. Optimized urine ELISA protocols using different antigens yielded higher prevalence rates and showed potential as alternative diagnostic methods. The results supported the utility of urine specimens for the diagnosis of strongyloidiasis.
Article
Infectious Diseases
Maria A. Pakharukova, Ekaterina Lishai, Oxana Zaparina, Nina Baginskaya, Sung-Jong Hong, Banchob Sripa, Viatcheslav Mordvinov
Summary: This study found that three types of parasitic infections (O. felineus, O. viverrini, and C. sinensis) can cause diseases in the hepatobiliary system, with O. viverrini having the greatest impact on the bile microbiome and O. felineus having the smallest impact on both bile and feces microbiomes. These findings can contribute to the development of more effective species-specific modalities for diagnosis, prevention, and treatment.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Microbiology
Maria N. Lvova, Denis V. Ponomarev, Alena A. Tarasenko, Anna V. Kovner, Galina A. Minkova, Michail A. Tsyganov, Meijun Li, Yan Lou, Veronica I. Evseenko, Alexander V. Dushkin, Irina V. Sorokina, Tatiana G. Tolstikova, Viatcheslav A. Mordvinov, Damira F. Avgustinovich
Summary: A micellar complex of curcumin with glycyrrhizic acid was effective in inhibiting Opisthorchis felineus, although its effect was weaker than that of praziquantel. The complex activated bile acid synthesis genes, reducing liver inflammation and periductal fibrosis.
Article
Biochemical Research Methods
Maria Y. Pakharukova, Ekaterina Savina, Dmitry V. Ponomarev, Natalya V. Gubanova, Oxana Zaparina, Elvira G. Zakirova, Guofeng Cheng, Olga V. Tikhonova, Viatcheslav A. Mordvinov
Summary: This study describes for the first time the release of extracellular vesicles (EVs) by the food-borne trematode Opisthorchis felineus, and characterizes their microscopic and proteomic properties, as well as their internalization pathways by human cholangiocytes. The differential abundance of proteins between whole adult worms and EVs was assessed, revealing enrichment of canonical EV markers and parasite specific proteins. These findings provide insights into the important role of EV cargo components in the pathogenesis of fluke infection and bile duct neoplasia.
JOURNAL OF PROTEOMICS
(2023)
Article
Agriculture, Dairy & Animal Science
Chairat Tantrawatpan, Wanchai Maleewong, Tongjit Thanchomnang, Warayutt Pilap, Takeshi Agatsuma, Ross H. Andrews, Paiboon Sithithaworn, Weerachai Saijuntha
Summary: This study aimed to characterize the intron regions of taurocyamine kinase gene (TK) in Opisthorchis viverrini and Clonorchis sinensis, and investigate their genetic variation and population genetics. The researchers found high nucleotide and haplotype diversity in O. viverrini samples, and observed heterozygous patterns specifically in humans with C. sinensis. They suggested that TkD1Int5 could be a high polymorphic genetic marker for further population genetic investigations of these liver flukes and related species.
Article
Multidisciplinary Sciences
Anna V. Kovner, Alena A. Tarasenko, Oxana Zaparina, Olga V. Tikhonova, Maria Y. Pakharukova, Viatcheslav A. Mordvinov
Summary: In this study, the wound healing potential of Opisthorchis felineus proteins in a mouse model was investigated. The results showed that these proteins could significantly enhance wound healing by reducing inflammation, modulating vascular response, and promoting dermal extracellular matrix remodeling.
SCIENTIFIC REPORTS
(2022)
Article
Parasitology
O. Pitaksakulrat, P. Sithithaworn, K. Y. Kopolrat, N. Kiatsopit, W. Saijuntha, R. H. Andrews, T. N. Petney, D. Blair
Summary: Digenetic trematodes are important parasites of humans and animals, infecting a gastropod as the first intermediate host. The morphological details of cercariae provide limited identification, so molecular data was used to improve identification and investigate the diversity of trematode species infecting B. s. goniomphalos in Thailand.
JOURNAL OF HELMINTHOLOGY
(2022)
Article
Parasitology
Maria Y. Pakharukova, Oxana Zaparina, Nina Baginskaya, Viatcheslav A. Mordvinov
Summary: This study used mRNA sequencing technology to investigate the changes in gene expression in the host liver infected with the food-borne trematode Opisthorchis felineus. The study found that the activated gene sets of cellular pathways differed between acute and chronic opisthorchiasis. Additionally, epithelial-mesenchymal transition was the most enriched gene set in all infected animals.
FOOD AND WATERBORNE PARASITOLOGY
(2022)
