Journal
PARASITOLOGY INTERNATIONAL
Volume 61, Issue 3, Pages 481-486Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.parint.2012.04.001
Keywords
Toxoplasma gondii; Adenovirus; Heterologous prime-boost; TgAMA1; DNA vaccine
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Funding
- Global COE Program from the Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Grants-in-Aid for Scientific Research [22780258] Funding Source: KAKEN
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A heterologous prime-boost strategy with priming plasmid DNA followed by recombinant virus expressing relevant antigens is known to stimulate protective immunity against intracellular parasites. In this study, we have evaluated a heterologous prime-boost strategy for immunizing mice against Toxoplasma gondii infection. Our results revealed that the prime-boost strategy using both plasmid DNA and adenoviral vector encoding TgAMA1 may stimulate both humoral and Th1/Th2 cellular immune responses specific for TgAMA1. Moreover, C57BL/6 mice immunized with the pAMA1/Ad5Null, pNull/Ad5AMA1, and pAMA1/Ad5AMA1 constructs showed survival rates of 12.5%, 37.5%, and 50%, respectively. In contrast, all the pNull/Ad5Null immunized mice died after infection with the PLK-GFP strain of T. gondii. Brain cyst burden was reduced by 23% in mice immunized with pAMA1/Ad5AMA1 compared with the pNull/Ad5AMA1 immunized mice. These results demonstrate that the heterologous DNA priming and recombinant adenovirus boost strategy may provide protective immunity against T. gondii infection. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
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