Article
Oncology
Martha E. Luitje, Anna-Karoline Israel, Michael A. Cummings, Ellen J. Giampoli, Paul D. Allen, Shawn D. Newlands, Catherine E. Ovitt
Summary: This study investigated the effects of radiation therapy on human salivary glands, finding that acinar cell loss was observed after radiation but regenerative potential remained. Changes in cell markers were seen in irradiated glands, suggesting potential impacts on regeneration mechanisms.
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2021)
Article
Toxicology
Yi Chen, Dan Tang, Hongping Wu, Yuling Wu, Tianjie Yuan, Hongdan Zhang, Yingfu Jiao, Weifeng Yu, Hexin Yan
Summary: In this study, a conditioned culture system was developed to maintain hepatocyte morphology and functions over the long term. The culture model showed the ability to stably preserve hepatic functions and can be used to assess drug-induced hepatotoxicity in vitro, providing a promising model for long-term toxicological studies and drug development.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Materials Science, Multidisciplinary
Rahul Rimal, Yvonne Marquardt, Thomas Nevolianis, Suzana Djeljadini, Andrea Bonnin Marquez, Sebastian Huth, Dmitry N. Chigrin, Matthias Wessling, Jens M. Baron, Martin Moeller, Smriti Singh
Summary: Utilizing a dynamic flow culture system improved the stability of scaffold-free vHSEs, enhancing skin barrier properties and promoting thicker tissue fabrication and wound closure. Balancing the expression of MMPs and their inhibitors in dynamic flow culture restored tissue homeostasis by modulating angiogenesis.
APPLIED MATERIALS TODAY
(2021)
Article
Agriculture, Dairy & Animal Science
Fahar Ibtisham, Tat-Chuan Cham, Mohammad Amin Fayaz, Ali Honaramooz
Summary: One out of every 650 children is affected by cancer, and about half of survivors become permanently infertile due to the side effects of cancer treatments. Pre-treatment testicular biopsies can be collected and cryopreserved for future use to uphold biological fatherhood. This study explored optimal conditions for the long-term culture of neonatal porcine testicular tissues as a first step in achieving in vitro spermatogenesis (IVS) in pigs.
Article
Multidisciplinary Sciences
Mariliis Klaas, Kaidi Moll, Kristina Maemets-Allas, Mart Loog, Martin Jarvekulg, Viljar Jaks
Summary: Solution blow spinning (SBS) combined with gelatin is an effective method to generate nano- and microfiber structures suitable for tissue engineering. 3D gelatin scaffolds generated by SBS were used as culturing substrates for creating 3D human liver tissue equivalents in 96-well format, supporting hepatocyte-specific gene expression and maintaining hepatocyte differentiation and polarization status in long term cultures.
SCIENTIFIC REPORTS
(2021)
Article
Biology
Timothy M. Waring, Zachary T. Wood
Summary: There is a potential evolutionary transition in individuality within the human species, with culture appearing to have greater adaptive potential than genes and likely driving human evolution. Culturally organized groups have come to dominate human affairs and culture's role is growing, bypassing genetic evolution and weakening genetic adaptive potential. This suggests an ongoing transition from genetic inheritance to cultural inheritance and a shift from genetic individuality to cultural group identity in human long-term gene-culture coevolution.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2021)
Article
Computer Science, Interdisciplinary Applications
Yang Hu, Xuewen Miao, Jun Zhang, Jie Liu, Ershun Pan
Summary: This paper proposes a novel reinforcement learning-driven aircraft maintenance strategy, which can provide real-time maintenance decisions based on aircraft's future mission requirements, repair costs, spare parts storage, and PHM outputs. Testing in simulated scenarios demonstrates that the strategy can better adapt to the varying requirements of different maintenance situations.
COMPUTERS & INDUSTRIAL ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Tatsuya Kometani, Koki Kamo, Taketomo Kido, Nobuyoshi Hiraoka, Taku Chibazakura, Kenji Unno, Keisuke Sekine
Summary: Pancreatic ductal adenocarcinoma (PDAC) is a serious and chemotherapy-resistant disease. We established a co-culture system using PDAC patient-derived cancer cells and stellate cells from human induced pluripotent stem cells to mimic the tumor microenvironment (TME) of PDAC. This system successfully demonstrated the interaction between cancer cells and stellate cells and replicated some features of PDAC in vitro. Furthermore, the co-culture system showed potential for drug treatment testing in vitro.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Amanda Marchini, Maria Gessica Ciulla, Barbara Antonioli, Alessandro Agnoli, Umberto Bovio, Virginia Visnoviz, Federico Bertuzzi, Fabrizio Gelain
Summary: Human pancreatic islets transplantation is an experimental treatment for Type I Diabetes, but limited lifespan in culture remains a challenge. This study proposes the use of self-assembling peptides to recreate a pancreatic extracellular matrix and provide mechanical and biological support to human pancreatic islets in vitro. The results show that the three-dimensional support provided by the HYDROSAP scaffold maintains islet functionality and morphology for up to 4 weeks, similar to freshly-isolated islets. Preliminary data suggests that pre-cultured islets in HYDROSAP hydrogels can restore normoglycemia in diabetic mice when transplanted.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Marie Cuvellier, Sophie Rose, Frederic Ezan, Ulrich Jarry, Hugo de Oliveira, Arnaud Bruyere, Christophe Drieu La Rochelle, Vincent Legagneux, Sophie Langouet, Georges Baffet
Summary: In recent decades, the development of 3D in vitro cultures of primary human hepatocytes (PHHs) has become increasingly important for mimicking liver functions. This study utilized 3D bioprinting technology to print PHH in a GelMa matrix, resulting in the rapid organization of polarized hollow spheroids that remained viable for at least 28 days. The bioprinted PHH exhibited high differentiation and maintained liver-specific functions over time, including the activities of phase I and II biotransformation enzymes. The study also demonstrated the ability of the bioprinted structures to vascularize when implanted in mice. This model shows promise for generating human liver tissue and advancing biotechnological developments.
Article
Pharmacology & Pharmacy
Goknil Pelin Coskun, Yagmur Ozhan, Vladimir Dobricic, Jelena Boskovic, Rengin Reis, Hande Sipahi, Zafer Sahin, Seref Demirayak
Summary: In this study, ten new diarylthiophene-2-carbohydrazide derivatives were synthesized and evaluated for their pharmacological activity. Compounds 7a, 7d, and 7f showed promising anticancer activity against pancreatic cancer cell line. However, the compounds exhibited lower COX-2 and 5-LOX inhibitory activity compared to the standard drugs. Molecular docking studies revealed the binding modes of compounds 7c, 7e, and 7f to the 5-LOX enzyme were non-redox or redox types, but not the iron-binding type. Among the compounds, 7a and 7f were identified as the most promising dual inhibitors of 5-LOX and pancreatic cancer cell line.
Article
Physiology
Maximilian J. Klumm, Christian Heim, Dominik J. Fiegle, Michael Weyand, Tilmann Volk, Thomas Seidel
Summary: This study presents a method for long-term cultivation of beating human atrial myocardium. The authors obtained tissues from the right-atrial appendage of patients with sinus rhythm and cultivated trabeculae for up to 3 weeks. They analyzed the functional parameters, gene expression, and tissue microstructure, and confirmed the viability and electromechanical function of the cultivated myocardium. This method allows for studying the physiology and pathophysiology of human atrial myocardium.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Horticulture
Carlos Lopez Encina, Manuel Lopez Granero, Jose Javier Regalado
Summary: In this article, the authors present a reliable method for the long-term micropropagation of papaya, using microshoots developed from the axillary buds of papaya shoots as primary explants. The proliferation medium (PPRM) was found to maintain papaya shoots under continuous proliferation for 20 years, while the elongation medium (PELM) and rooting medium (PROM) were designed to recover shoots with poor growth and induce high-quality roots from nonrooted shoots, respectively. The main objective of this work was to provide a micropropagation method that can maintain healthy elite genotypes of papaya for long periods of time and produce a high number of good quality plants.
Article
Biochemistry & Molecular Biology
Go Sugahara, Yuji Ishida, Jae Jin Lee, Meng Li, Yasuhito Tanaka, Hyungjin Eoh, Yusuke Higuchi, Takeshi Saito
Summary: This study aimed to define the environmental requirements necessary for maintaining the homeostasis of terminally differentiated hepatocytes. The supplementation of dimethyl sulfoxide (DMSO) was found to be indispensable for mitigating fate deterioration and promoting adaptation to the in vitro environment. Dimethyl sulfone (DMSO2) was identified as a substitute for DMSO, supporting the long-term maintenance of hepatocyte morphology, marker gene expression, and functionality.
Article
Biochemistry & Molecular Biology
Myroslava Mytsyk, Giulia Cerino, Gregory Reid, Laia Gili Sole, Friedrich S. Eckstein, David Santer, Anna Marsano
Summary: The study showed that SVF cells cultured in hypoxic conditions increased the release of vascular endothelial growth factor and the number of proliferating cells, as well as enhanced vessel length density in engineered tissues after 28 days of implantation. Compared to normoxia, the number of human cells and proliferating cells in hypoxia-cultured constructs significantly increased after 3 and 28 days in vivo. These findings suggest that hypoxic culture may not impair the vascularization potential of SVF patches in a myocardial ischemia model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
