Article
Biochemistry & Molecular Biology
Young Yun Jung, Chakrabhavi Dhananjaya Mohan, Huiyan Eng, Acharan S. Narula, Ojas A. Namjoshi, Bruce E. Blough, Kanchugarakoppal S. Rangappa, Gautam Sethi, Alan Prem Kumar, Kwang Seok Ahn
Summary: This study demonstrates that 2,3,5,6-Tetramethylpyrazine (TMP) can suppress the EMT program in colon cancer cells by regulating MnSOD and related proteins, and inhibit the proliferation, migration, and invasion of tumor cells.
Article
Medicine, Research & Experimental
Yan Sun, Lang Xiong, Xiaoran Wang, Liping Wang, Baoxin Chen, Jingqi Huang, Mi Huang, Jieping Chen, Jing Wu, Shan Huang, Yizhi Liu
Summary: This study demonstrated that TGF-beta 2 induces autophagy flux during EMT progression of LECs, with autophagy activation enhancing fibrogenic responses and cell migration, while autophagy inhibition alleviates EMT markers and cell migration. Additionally, modulation of Smad signaling by autophagy was observed, and ATG7-silenced LECs showed antifibrosis effect induced by TGF-beta 2. Overall, intervention/inhibition of autophagy could attenuate TGF-beta 2-induced EMT in LECs, providing insights for preventing and treating fibrotic cataracts and other fibrotic diseases.
Article
Cell Biology
Sham Jdeed, Mate Lengyel, Ivan P. Uray
Summary: This study reveals the chromatin-modulating mechanisms of ARID1A in breast epithelial cells and its suppression effects on genes related to TGF-beta signaling, which is of great significance for cancer prevention.
Article
Biochemistry & Molecular Biology
Soo-Jin Park, Tae-hyoun Kim, Kiram Lee, Min-Ah Kang, Hyun-Jae Jang, Hyung-Won Ryu, Sei-Ryang Oh, Hyun-Jun Lee
Summary: Idiopathic pulmonary fibrosis (IPF) is a difficult-to-treat interstitial lung disease with no effective treatment currently available. Kurarinone, a flavonoid isolated from Sophora Flavescens, has shown therapeutic potential in attenuating lung fibrosis by suppressing TGF-beta signaling pathways. This study suggests that Kurarinone may serve as a novel drug candidate for pulmonary fibrosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Cheng Xiong, Youwei Zhu, Meilin Xue, Yongsheng Jiang, Yiming Zhong, Lingxi Jiang, Minmin Shi, Hao Chen
Summary: This study revealed that TAMs promote PDAC progression through the TGF-beta signaling pathway, and the pro-tumorigenic effects of TAMs or TAM-CM can be abolished by inhibiting the TGF-beta signaling pathway or neutralizing TGF-beta antibody.
Article
Biochemistry & Molecular Biology
Lauren A. Griggs, Christopher A. Lemmon
Summary: Epithelial to Mesenchymal Transition (EMT) is a process in which epithelial cells transition to a motile and invasive mesenchymal phenotype. The EMT process is associated with an increase in fibronectin (FN) assembly and the localization of Transforming Growth Factor-beta 1 (TGF-beta 1) to FN fibrils. This study investigated the spatial gradients of EMT and FN fibrillogenesis in mammary epithelial cells cultured on square micropatterns in response to TGF-beta 1. The results showed that increasing TGF-beta 1 concentration induced a spatial gradient of both E-Cadherin and FN fibrils, and inhibition of FN fibril assembly globally reduced both E-Cadherin-containing adherens junctions and FN fibrillogenesis, but did not eliminate the spatial gradients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Xuke Qin, Lin Liu, Yanze Li, Hongbo Luo, Hui Chen, Xiaodong Weng
Summary: GOLM1 is upregulated in prostate cancer and promotes migration and invasion abilities by activating the TGF-beta 1/Smad2 signaling pathway to drive epithelial-mesenchymal transition (EMT). Therefore, GOLM1 has the potential to serve as a biomarker for prostate cancer diagnosis and prognosis prediction.
TECHNOLOGY IN CANCER RESEARCH & TREATMENT
(2023)
Article
Cell & Tissue Engineering
Le Phuong Hoang Anh, Ken Nishimura, Akihiro Kuno, Nguyen Thuy Linh, Tetsuo Kato, Manami Ohtaka, Mahito Nakanishi, Eiji Sugihara, Taka-Aki Sato, Yohei Hayashi, Aya Fukuda, Koji Hisatake
Summary: Somatic cell reprogramming proceeds through a series of events to generate induced pluripotent stem cells (iPSCs). In this study, the researchers found that the downregulation of Osr2 is crucial for efficient reprogramming by using a cell-based model. Osr2 acts as a novel EMT regulator and its downregulation diminishes TGF-beta signaling and activates Wnt signaling, promoting MET and acquiring pluripotency.
Article
Biochemistry & Molecular Biology
Kai-Zhou Jin, Ying Wu, Xiao-Xiao Zheng, Tian-Jiao Li, Zhen-Yu Liao, Qing-Lin Fei, Hui-Ru Zhang, Sai-Meng Shi, Xin Sha, Xian-Jun Yu, Wei Chen, Long-Yun Ye, Wei-Ding Wu
Summary: Intrinsic drug resistance mechanisms in tumor cells and epithelial-to-mesenchymal transition (EMT) significantly reduce the effectiveness of cancer treatment. This study developed cSN38 nanoparticles and a TGF-beta 1 inhibitor, LY364947, as a strategy to overcome drug resistance in pancreatic ductal adenocarcinoma (PDAC). The combination of cSN38 and LY364947 nanoparticles improved drug sensitivity, reduced EMT, and inhibited PDAC tumor growth both in vitro and in vivo. These findings highlight the potential of nanoscale therapeutics in combating PDAC.
Article
Biochemistry & Molecular Biology
Guangwen Shu, Arslan Yusuf, Chenxi Dai, Hui Sun, Xukun Deng
Summary: PIP exhibits anti-fibrotic and hepatoprotective effects in both cells and mouse liver, possibly through activation of the Nrf2 signaling pathway and inhibition of the TGF-beta 1/Smad pathway.
Article
Oncology
Hyun-Wook Lee, Cynthia C. Jose, Suresh Cuddapah
Summary: Nickel compounds, prevalent in the atmosphere due to industrial processes and fossil fuel burning, are associated with chronic inflammatory lung diseases, including asthma and lung cancer, primarily through the mechanism of epithelial-mesenchymal transition (EMT).
SEMINARS IN CANCER BIOLOGY
(2021)
Article
Biophysics
Naba Mukhtar, Eric N. Cytrynbaum, Leah Edelstein-Keshet
Summary: Epithelial-mesenchymal transition (EMT) plays a crucial role in wound healing, embryonic development, and cancer metastasis. This study presents a multiscale model that investigates the role of the protein YAP in promoting EMT and validates the simulation results with experimental observations.
BIOPHYSICAL JOURNAL
(2022)
Article
Oncology
Feifeng Lan, Menghua Chen, Xiaowei Xie, Yanyan Mo, Fengti Chen, Renbin Huang, Wenqi Liu
Summary: YLSPS, derived from the root of Millettia pulchra var. laxior, exhibits anticancer effects in lung cancer cells by reversing EMT and inhibiting the TGF-beta 1-induced ERK signaling pathway. YLSPS significantly inhibits the growth of xenograft tumors and reduces the levels of proteins associated with EMT. Importantly, YLSPS has fewer toxic side effects than cisplatin.
AMERICAN JOURNAL OF CANCER RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Long Xu, Jian Wang, Buhan Liu, Jiaying Fu, Yuanxin Zhao, Sihang Yu, Luyan Shen, Xiaoyu Yan, Jing Su
Summary: Epithelial ovarian cancer has the highest mortality rate among gynecological malignant tumors, largely due to metastasis. This study found that HDAC9 plays different roles in different histological subtypes of ovarian cancer and may be a potential target for personalized treatment.
Article
Chemistry, Medicinal
Yifan Wang, Yixiang Wang, Yang Wu, Yiqing Wang
Summary: The study aimed to investigate the effect of dulaglutide on intrauterine adhesion (IUA) and elucidate its mechanism for potential clinical treatment of IUA. The findings showed that dulaglutide improved uterine cavity shape, increased endometrial thickness and gland number, and significantly reduced collagen fiber deposition in the endometrium. It also reduced inflammatory responses, inhibited M1 macrophage polarization and inflammatory factor release, and ameliorated fibrosis via inhibiting epithelial-mesenchymal transition (EMT) through the TGF-beta/Smad2 signaling pathway.
Article
Medicine, Research & Experimental
Lieke Joosten, Maarten Brom, Hanneke Peeters, Desiree Bos, Eddy Himpe, Luc Bouwens, Otto Boerman, Martin Gotthardt
MOLECULAR PHARMACEUTICS
(2019)
Article
Food Science & Technology
Celine Moens, Mohammed Bensellam, Eddy Himpe, Christo J. F. Muller, Jean-Christophe Jonas, Luc Bouwens
MOLECULAR NUTRITION & FOOD RESEARCH
(2020)
Retraction
Biotechnology & Applied Microbiology
Luc Baeyens, Marie Lemper, Gunter Leuckx, Sofie De Groef, Paola Bonfanti, Geert Stange, Ruth Shemer, Christoffer Nord, David W. Scheel, Fong C. Pan, Ulf Ahlgren, Guoqiang Gu, Doris A. Stoffers, Yuval Dor, Jorge Ferrer, Gerard Gradwohl, Christopher V. E. Wright, Mark Van de Casteele, Michael S. German, Luc Bouwens, Harry Heimberg
NATURE BIOTECHNOLOGY
(2020)
Article
Oncology
Mohamad Assi, Younes Achouri, Axelle Loriot, Nicolas Dauguet, Hajar Dahou, Jonathan Baldan, Maxime Libert, Jean S. Fain, Carmen Guerra, Luc Bouwens, Mariano Barbacid, Frederic P. Lemaigre, Patrick Jacquemin
Summary: The study demonstrates that pancreatic acinar cells become less sensitive to tumorigenesis induced by oncogenic Kras mutations after birth, due to low expression of KRAS and its effectors. Pancreatitis induces the expression of KRAS and its effectors, requiring the activity of EGFR. Expression of C-RAF in adult pancreas is essential for pancreatic tumorigenesis.
Article
Biochemistry & Molecular Biology
Elyne Backx, Elke Wauters, Jonathan Baldan, Mathias Van Bulck, Ellis Michiels, Yves Heremans, Diedert Luc De Paep, Mineo Kurokawa, Susumu Goyama, Luc Bouwens, Patrick Jacquemin, Isabelle Houbracken, Ilse Rooman
Summary: This study transcriptionally profiled the two major human pancreatic exocrine cell types, acinar and duct cells, during experimental stress conditions. The results showed that in dedifferentiated acinar cells, cancer pathways are upregulated in which MECOM is a critical regulator that suppresses acinar cell death by permitting cellular dedifferentiation.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Oncology
Eva Reijmen, Sven De Mey, Wout De Mey, Thierry Gevaert, Kirsten De Ridder, Hanne Locy, Sandrina Martens, Emmy De Blay, Luc Bouwens, Pieterjan Debie, Karine Breckpot, Jacques De Greve, Mark De Ridder, Cleo Goyvaerts
Summary: The combination of low-dose fractionated radiation therapy with immunotherapy in lung cancer treatment affects the therapeutic outcome and immune cells. Reprogramming of immune cells may enhance the treatment efficacy in patients with non-small cell lung cancer receiving fractionated radiation therapy.
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2021)
Article
Neurosciences
Ana Rita Pombo Antunes, Isabelle Scheyltjens, Francesca Lodi, Julie Messiaen, Asier Antoranz, Johnny Duerinck, Daliya Kancheva, Liesbet Martens, Karen De Vlaminck, Hannah Van Hove, Signe Schmidt Kjolner Hansen, Francesca Maria Bosisio, Koen Van der Borght, Steven De Vleeschouwer, Raf Sciot, Luc Bouwens, Michiel Verfaillie, Niels Vandamme, Roosmarijn E. Vandenbroucke, Olivier De Wever, Yvan Saeys, Martin Guilliams, Conny Gysemans, Bart Neyns, Frederik De Smet, Diether Lambrechts, Jo A. Van Ginderachter, Kiavash Movahedi
Summary: By utilizing single-cell RNA sequencing and CITE-seq, this study reveals the complexity of the immune landscape in glioblastomas, including conserved populations of dendritic cells and macrophages. The results indicate that microglia-derived tumor-associated macrophages dominate in newly diagnosed tumors, but are replaced by monocyte-derived TAMs in recurrences.
NATURE NEUROSCIENCE
(2021)
Letter
Gastroenterology & Hepatology
H. Buckens, S. Pirenne, Y. Achouri, J. Baldan, H. Dahou, L. Bouwens, F. P. Lemaigre, P. Jacquemin, M. Assi
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Gastroenterology & Hepatology
Sandrina Martens, Katarina Coolens, Mathias Van Bulck, Tatjana Arsenijevic, Joan Casamitjana, Angel Fernandez Ruiz, Abdessamad El Kaoutari, Jaime Martinez de Villareal, Hediel Madhloum, Farzad Esni, Yves Heremans, Gunter Leuckx, Harry Heimberg, Luc Bouwens, Patrick Jacquemin, Diedert Luc De Paep, Peter in't Veld, Nicky D'Haene, Christelle Bouchart, Nelson Dusetti, Jean-Luc Van Laethem, Wim Waelput, Pierre Lefesvre, Francisco X. Real, Meritxell Rovira, Ilse Rooman
Summary: In larger human pancreatic ducts, basal cells exist. Delta Np63 suppresses duct cell identity. These cells may play an important role in pancreatic disease, including PDAC ontogeny, but are not present in mouse models.
Article
Immunology
Eva Reijmen, Sven De Mey, Helena Van Damme, Kirsten De Ridder, Thierry Gevaert, Emmy De Blay, Luc Bouwens, Christine Collen, Lore Decoster, Marijke De Couck, Damya Laoui, Jacques De Greve, Mark De Ridder, Yori Gidron, Cleo Goyvaerts
Summary: The combination of radiotherapy with immunotherapy shows promise for NSCLC patients, with room for optimization to improve response rates. Preclinical studies suggest that VNS alone or combined with RT can enhance the activation profile of intratumoral CD8(+) T cells, while reducing the levels of regulatory T cells and monocytes in the periphery. These findings support further research into the use of non-invasive VNS to optimize response rates in lung cancer patients.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Celine Moens, Christo J. F. Muller, Luc Bouwens
Summary: This study aimed to compare the protective effects of Rooibos components and reference molecules against lipotoxicity and oxidative stress in pancreatic beta cells. The results showed that Rooibos components offered significant protection and were more effective than the reference molecules.
Article
Immunology
Karen De Vlaminck, Hannah Van Hove, Daliya Kancheva, Isabelle Scheyltjens, Ana Rita Pombo Antunes, Jonathan Bastos, Monica Vara-Perez, Leen Ali, Myrthe Mampay, Lauren Deneyer, Juliana Fabiani Miranda, Ruiyao Cai, Luc Bouwens, Dimitri De Bundel, Guy Caljon, Benoit Stijlemans, Ann Massie, Jo A. Van Ginderachter, Roosmarijn E. Vandenbroucke, Kiavash Movahedi
Summary: By examining the fate of microglia, border-associated macrophages (BAMs), and recruited macrophages during neuroinflammation and resolution, it was found that their responses and dynamics differ.
Meeting Abstract
Gastroenterology & Hepatology
E. Backx, E. Wauters, J. Baldan, M. Van Bulck, E. Michiels, Y. Heremans, D. L. De Paep, L. Bouwens, M. Kurokawa, S. Goyama, P. Jacquemin, I. Houbracken, I. Rooman
Meeting Abstract
Oncology
Mohamad Nabil Assi, Jean-Nicolas Lodewyckx, Younes Achouri, Claude Gerard, Nicolas Dauguet, Isabelle Houbracken, Yves Heremans, Donatienne Tyteca, Ilse Rooman, Luc Bouwens, Frederic Lemaigre, Patrick Jacquemin
MOLECULAR CANCER RESEARCH
(2020)
Article
Multidisciplinary Sciences
Jonathan Baldan, Isabelle Houbracken, Ilse Rooman, Luc Bouwens
SCIENTIFIC REPORTS
(2019)