Article
Immunology
Yamini Chelpuri, Shivakumar Pabbathi, Gopala Reddy Alla, Ravi Kumar Yadala, Mounika Kamishetti, Anil Kumar Banothu, Ramya Boinepally, Kala Kumar Bharani, Amit Khurana
Summary: Hinokitiol has significant potential in preventing cerulein-induced acute pancreatitis by reducing pancreatic edema, lowering plasma enzyme levels, attenuating oxidative and nitrosative stress, improving antioxidant enzyme activity, and regulating inflammatory cytokine levels.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Marcus Hollenbach, Sebastian Sonnenberg, Ines Sommerer, Jana Lorenz, Albrecht Hoffmeister
Summary: AR42J cells serve as a reliable in-vitro model for CN-induced AP, but specific conditions are required for reproducible data. Dexamethasone treatment is essential for differentiation to an acinar-cell-like phenotype and amylase production in AR42J. CN induces amylase secretion without affecting amylase production, with peak secretion levels observed between 3 and 24 hours of incubation.
Article
Biochemistry & Molecular Biology
Katarzyna Konarska-Bajda, Piotr Ceranowicz, Jakub Cieszkowski, Grzegorz Ginter, Agnieszka Stempniewicz, Krystyna Galazka, Beata Kusnierz-Cabala, Paulina Dumnicka, Joanna Bonior, Zygmunt Warzecha
Summary: Acute pancreatitis is a severe disease characterized by inflammation and coagulation. Anticoagulants have been suggested as a potential therapeutic option. This study investigated the effects of pretreatment with warfarin on the development of edematous pancreatitis in mice. The results showed that pretreatment with low doses of warfarin reduced pancreatic damage and improved various parameters of pancreatitis. However, high doses of warfarin did not exhibit a preventive effect.
Article
Biochemistry & Molecular Biology
Marcus Hollenbach, Sebastian Sonnenberg, Ines Sommerer, Jana Lorenz, Albrecht Hoffmeister
Summary: Inflammation caused by oxidative stress plays an important role in the pathogenesis of acute pancreatitis, with MGO and AGEs being key sources of ROS. Modulation of Glo-I can reduce the release of pro-inflammatory cytokines in CN-induced AP models.
Article
Environmental Sciences
Sapana Bansod, Shrilekha Chilvery, Mohd Aslam Saifi, Tridip Jyoti Das, Hui Tag, Chandraiah Godugu
Summary: The study demonstrates that borneol has a protective effect in cerulein-induced acute pancreatitis, reducing pancreatic damage, oxidative stress, and inflammation by modulating the Nrf2/NF-κB pathway.
ENVIRONMENTAL TOXICOLOGY
(2021)
Article
Cell Biology
Heng-Fu Bu, Saravanan Subramanian, Hua Geng, Xiao Wang, Fangyi Liu, Pauline M. Chou, Chao Du, Isabelle G. De Plaen, Xiao-Di Tan
Summary: The study demonstrates that MFG-E8 plays a protective role in the pancreas and promotes tissue repair during acute pancreatitis. Knocking out MFG-E8 exacerbates the severity of pancreatitis, while administration of recombinant MFG-E8 can attenuate acute pancreatitis and facilitate pancreatic injury repair.
Article
Gastroenterology & Hepatology
Zsanett Jancso, Miklos Sahin-Toth
Summary: T7K24R mice with a trypsinogen mutation develop chronic pancreatitis after cerulein-induced acute pancreatitis, characterized by acinar cell atrophy, persistent macrophage infiltration, and fibrosis. The trypsin-triggered inflammatory process plays a crucial role in the progression from pancreatitis to chronic disease.
Article
Biotechnology & Applied Microbiology
Jiandang Zhou, Qiao Tan, Jinxue Tong, Zhekuan Tong, Chunlu Wang, Bei Sun, Min Fang, Jiachen Lv
Summary: This research explored the mechanism by which PIAS1 affects acute pancreatitis (AP) and found that PIAS1 upregulation can diminish FTO transcription by increasing Foxa2 SUMO modification, thereby ameliorating Cerulein-induced AP. The study was conducted using Cerulein-induced AR42J cells and mice, and the results showed that Cerulein-induced AP cells and mice had low PIAS1 and Foxa2 expression and high FTO expression. The findings suggest a potential therapeutic target for the treatment of acute pancreatitis.
Article
Physiology
Mattea Durst, Theresia Reding Graf, Rolf Graf, Mareike Kron, Margarete Arras, Dietmar Zechner, Rupert Palme, Steven R. Talbot, Paulin Jirkof
Summary: The study investigated the impact of acute Cerulein-induced pancreatitis on mice and found that acute pain caused by Cerulein injections could not be alleviated by the tested analgesics. However, more severe or chronic pancreatitis protocols might induce more pain, necessitating further research for effective analgesics to alleviate acute pain induced by Cerulein injections.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ewa Tomaszewska, Malgorzata Swiatkiewicz, Siemowit Muszynski, Janine Donaldson, Katarzyna Ropka-Molik, Marcin B. Arciszewski, Maciej Murawski, Tomasz Schwarz, Piotr Dobrowolski, Sylwia Szymanczyk, Slawomir Dresler, Joanna Bonior
Summary: Chronic pancreatitis (CP) is an irreversible and progressive inflammatory disease. This study aimed to investigate the changes in pro-inflammatory cytokines, antioxidant defense system, and selected serum biochemical parameters in a cerulein-induced CP model in pigs. The study found that repetitive cerulein injections not only led to CP and induced inflammation in the intestine, but also impaired the intestinal barrier.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Vanice Paula Ricardo Carvalho, Juliana Figueira da Silva, Marcelo Araujo Buzelin, Claudio Antonio da Silva Junior, Duana Carvalho dos Santos, Danuza Montijo Diniz, Nancy Scardua Binda, Marcia Helena Borges, Andre Luiz Senna Guimaraes, Elizete Maria Rita Pereira, Marcus Vinicius Gomez
Summary: Agents modulating high-voltage gated calcium channels have significant effects in relieving visceral pain. The molecular mechanisms of chronic pain associated with acute pancreatitis are poorly understood, with hypercalcemia considered as a risk factor. Blocking calcium signals may be useful in prophylactic treatment of pancreatitis.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Surgery
Yin Xian, Yi Wu, Ming He, Junming Cheng, Xiaodong Lv, Yixing Ren
Summary: The study found that obese rats undergoing SG had significantly lower body weight, food intake, and blood lipids compared to the sham surgery group, and milder symptoms of pancreatitis. The conclusion shows that SG can reduce the severity of the first onset of pancreatitis and acute episodes of recurrent pancreatitis in obese rats, and improving lipid metabolism and body weight may play an important role.
Article
Oncology
Ram Vinod Roy, Nicolas Means, Geeta Rao, Sima Asfa, Venkateshwar Madka, Anindya Dey, Yushan Zhang, Monalisa Choudhury, Kar-Ming Fung, Danny N. Dhanasekaran, Jacob E. Friedman, Howard C. Crawford, Chinthalapally V. Rao, Resham Bhattacharya, Priyabrata Mukherjee
Summary: Ubiquitin-binding associated protein 2 (UBAP2) has been found to promote macropinocytosis and pancreatic adenocarcinoma growth. However, its role in normal pancreatic function was previously unknown. In this study, UBAP2 knockout mice were generated and it was found that they exhibited slight glucose intolerance and reduced levels of pancreatic enzymes and inflammation-related cytokines upon induction of pancreatitis. Furthermore, the decreased activation of the NF-kappa B pathway was observed in cerulein-challenged UBAP2 knockout mice. These findings suggest that UBAP2 may be a potential therapeutic target for inhibiting pancreatitis.
Article
Biochemistry & Molecular Biology
Mate Nagy-Penzes, Zoltan Hajnady, Zsolt Regdon, Mate A. Demeny, Katalin Kovacs, Tarek El-Hamoly, Jozsef Maleth, Peter Hegyi, Csaba Hegedus, Laszlo Virag
Summary: Acute pancreatitis is a worldwide challenge due to its increasing incidence and life-threatening course. The flavone compound, tricetin, shows potential protective effects in acute pancreatitis by reducing inflammation, necrosis, and oxidative DNA damage.
Article
Cell Biology
Yinghui Song, Zhihua Zhang, Zhangtao Yu, Guoyi Xia, Yizhi Wang, Le Wang, Chuang Peng, Bo Jiang, Sulai Liu
Summary: This study investigated the role of Wip1 in acute pancreatitis (AP) and found that Wip1 was upregulated in AP models, involved in autophagy and STING pathway activation. Inhibition of Wip1 counteracted the inflammatory response and reduced autophagy levels in AP, suggesting its potential as a target for AP treatment.
Article
Cell Biology
Arturo Mangas, Javier Yajeya, Noelia Gonzalez, Isabel Ruiz, Marianny Pernia, Michel Geffard, Rafael Covenas
EUROPEAN JOURNAL OF HISTOCHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Arturo Mangas, Javier Yajeya, Noelia Gonzalez, Marianne Husson, Michel Geffard, Rafael Covenas
FOLIA HISTOCHEMICA ET CYTOBIOLOGICA
(2016)
Article
Multidisciplinary Sciences
Samuel Collombet, Chris van Oevelen, Jose Luis Sardina Ortega, Wassim Abou-Jaoude, Bruno Di Stefano, Morgane Thomas-Chollier, Thomas Graf, Denis Thieffry
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Biochemical Research Methods
Violeta Garcia-Hernandez, Carmen Sanchez-Bernal, Domitille Schvartz, Jose J. Calvo, Jean-Charles Sanchez, Jesus Sanchez-Yague
JOURNAL OF PROTEOMICS
(2018)
Article
Cell & Tissue Engineering
Jose Luis Sardina, Samuel Collombet, Tian Tian, Antonio Gomez, Bruno Di Stefano, Clara Berenguer, Justin Brumbaugh, Ralph Stadhouders, Carolina Segura-Morales, Marta Gut, Ivo G. Gut, Simon Heath, Sergi Aranda, Luciano Di Croce, Konrad Hochedlinger, Denis Thieffry, Thomas Graf
Article
Cell Biology
Arturo Mangas, Margarita Heredia, Adelaida Riolobos, Antonio de la Fuente, Jose Maria Criado, Javier Yajeya, Michel Geffard, Rafael Covenas
EUROPEAN JOURNAL OF HISTOCHEMISTRY
(2018)
Article
Cell Biology
Tian Tian, Bruno Di Stefano, Gregoire Stik, Maria Vila-Casadesus, Jose Luis Sardina, Enrique Vidal, Alessandro Dasti, Carolina Segura-Morales, Luisa De Andres-Aguayo, Antonio Gomez, Johanna Goldmann, Rudolf Jaenisch, Thomas Graf
NATURE CELL BIOLOGY
(2019)
Article
Hematology
Yan Xiu, Qianze Dong, Lin Fu, Aaron Bossler, Xiaobing Tang, Brendan Boyce, Nicholas Borcherding, Mariah Leidinger, Jose Luis Sardina, Hai-hui Xue, Qingchang Li, Andrew Feldman, Iannis Aifantis, Francesco Boccalatte, Lili Wang, Meiling Jin, Joseph Khoury, Wei Wang, Shimin Hu, Youzhong Yuan, Endi Wang, Ji Yuan, Siegfried Janz, John Colgan, Hasem Habelhah, Thomas Waldschmidt, Markus Muschen, Adam Bagg, Benjamin Darbro, Chen Zhao
Article
Biochemistry & Molecular Biology
Pablo Sanchez, Manuel Lisardo Sanchez, Arturo Mangas, Luis Angel Aguilar, Rafael Covenas
FOLIA HISTOCHEMICA ET CYTOBIOLOGICA
(2020)
Review
Oncology
Aleksey Lazarenkov, Jose Luis Sardina
Summary: This review summarizes the latest research on the function of the TET2 gene in hematopoietic cells and its importance in the origin and development of blood cancer. Mutations in TET2 lead to expansion of hematopoietic stem cells and eventually give rise to various types of blood malignancies. The focus of the research is to uncover the molecular mechanisms mediated by TET2 that activate aberrant transcriptional programs in the onset and development of blood cancer.
Article
Biotechnology & Applied Microbiology
Claudia Vivori, Panagiotis Papasaikas, Ralph Stadhouders, Bruno Di Stefano, Anna Ribo Rubio, Clara Berenguer Balaguer, Serena Generoso, Anna Mallol, Jose Luis Sardina, Bernhard Payer, Thomas Graf, Juan Valcarcel
Summary: This study investigates the dynamics of alternative splicing changes during reprogramming of mouse B cells and embryonic fibroblasts. Significant overlap in alternative splicing changes was observed in both reprogramming systems, independent of transcriptional levels. Identification of novel regulators and their targets provides insights into common splicing regulation programs during reprogramming of different cell types.
Article
Anatomy & Morphology
A. Mangas, J. Yajeya, N. Gonzalez, I. Ruiz, S. Duleu, M. Geffard, R. Covenas
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
(2017)
Article
Multidisciplinary Sciences
Violeta Garcia-Hernandez, Carmen Sanchez-Bernal, Domitille Schvartz, Jose J. Calvo, Jean-Charles Sanchez, Jesus Sanchez-Yague