Journal
TOXICOLOGY IN VITRO
Volume 29, Issue 3, Pages 479-488Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tiv.2014.12.022
Keywords
Cyanotoxin; Cylindrospermopsin; Cell lineage; HepG2; CYPs
Categories
Funding
- CNPq (Brazilian Agency for Science and Technology)
- CAPES
Ask authors/readers for more resources
Human hepatoma cells (HepG2) were exposed to purified cylindrospermopsin (CYN), a potent toxicant for eukaryotic cells produced by several cyanobacteria. CYN was not toxic at concentrations up to 10 mu g l(-1), leading to increased viability and metabolism in cells cultured with 10% fetal bovine serum (FBS). Reduction of FBS concentration to 2% and induction of cytochrome P450 (CYP) isoforms were performed in order to make xenobiotic-metabolizing capacity of HepG2 cells closest to that of 'normal' cells. HepG2 cells proliferated less after CYPs-induction, and this induction has lead to similar results of noninduced cells, except for few individual parameters such lipid peroxidation. Foremost, low concentrations of CYN (below or equal 10 mu g l(-1) have induced HepG2 cells proliferation and metabolism increase, which was not expected. (c) 2015 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available