Journal
OSTEOPOROSIS INTERNATIONAL
Volume 22, Issue 2, Pages 703-709Publisher
SPRINGER LONDON LTD
DOI: 10.1007/s00198-010-1240-3
Keywords
Bone gene expression; Bone properties; Osteoporosis; Phytoestrogen; Sambucus williamsii HANCE
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Funding
- State Key Laboratory of Chinese Medicine and Molecular Pharmacology
- Research Committee of the Hong Kong Polytechnic University [I-BB8N]
- Shenzhen-Hong Kong Innovation Circle
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The purpose of this study is to investigate the dose-dependent effects of SWH on bone properties and the mechanism involved in mediating the osteoprotective actions of SWH. The results indicated that SWH could improve bone properties by inhibiting the process of bone resorption and stimulating the process of bone formation. Our previous study showed that Sambucus williamsii HANCE (SWH) improved trabecular bone mass and cortical bone strength in ovariectomized (OVX) rats. The purpose of this study is to investigate the dose-dependent effects of SWH on bone properties and the mechanism involved in mediating the osteoprotective actions of SWH. Three-month-old C57BL/6J mice were fed a phytoestrogen-free diet and subjected to either ovariectomy or sham operation. OVX mice were treated with genistein (50 mg/kg), or a low (200 mg/kg), medium (500 mg/kg), or high (1,000 mg/kg) dose of SWH extract. SWH could dose-dependently decrease urinary Ca excretion and increase serum Ca level in OVX mice. It could increase tibial bone mineral density and exert beneficial effects on the microarchitecture of trabecular bone in the OVX mice. SWH suppressed the ovariectomy-induced expression of Cbfa1 mRNA and cathepsin K mRNA and enhanced the ratio of OPG/RANKL mRNA expression in the tibia. In vitro study showed that SWH dramatically reduced the number of TRAP-positive cells in RANKL-induced RAW 264.7 cells. The present study indicated that SWH could improve bone properties by inhibiting the process of bone resorption and stimulating the process of bone formation.
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