Article
Cell Biology
Joanna Smeeton, Natasha Natarajan, Troy Anderson, Kuo-Chang Tseng, Peter Fabian, J. Gage Crump
Summary: The poor intrinsic repair capacity of mammalian joint cartilage likely contributes to the high incidence of arthritis worldwide. Adult zebrafish can regenerate many structures that show limited or no healing capacity in mammals, including the jawbone. Researchers have found that zebrafish can regenerate adult joint cartilage and have identified specific cell types that may be involved in regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Dora Sapede, Sarah Bahraoui, Lea Abou Nassif, Audrey Barthelaix, Marc Mathieu, Christian Jorgensen, Farida Djouad
Summary: The study aimed to test the regenerative capacity of Meckel's cartilage (MC) in zebrafish after mechanical injury and identify the underlying mechanisms. Results showed that zebrafish could fully regenerate MC through chondrocyte cell cycle re-entry and proliferation of pre-existing MC chondrocytes. The Nrg1/ErbB pathway played a pivotal role in this regeneration process and showed potential for promoting cartilage regeneration in mammals.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Orthopedics
Genevieve Housman, Ellen E. Quillen, Anne C. Stone
Summary: This study compares DNA methylation variations and their relationship with knee osteoarthritis development between baboons and humans, revealing that cartilage epigenetics may play a more significant role in OA than bone epigenetics. Genes containing OA-related DMPs in baboons show similar methylation patterns to those in humans, indicating a mixture of evolutionarily conserved and divergent OA-related methylation patterns in primates.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2021)
Article
Cell & Tissue Engineering
Angela Ruscitto, Peng Chen, Ikue Tosa, Ziyi Wang, Gan Zhou, Ingrid Safina, Ran Wei, Mallory M. Morel, Alia Koch, Michael Forman, Gwendolyn Reeve, Michael K. Lecholop, Marshall Wilson, Daniel Bonthius, Mo Chen, Mitsuaki Ono, Timothy C. Wang, Hai Yao, Mildred C. Embree
Summary: In this study, we identified Lgr5-expressing secretory cells as forming a Wnt inhibitory niche that guides Wnt-inactive chondroprogenitors to form synovial joints and regulates chondrocyte lineage and identity. Furthermore, by injecting StemJEL, we were able to restore cartilage homeostasis, chondrocyte identity, and joint function in post-traumatic osteoarthritic jaw and knee joints.
Review
Biochemistry & Molecular Biology
Maria-Luisa Perez-Lozano, Annabelle Cesaro, Marija Mazor, Eric Esteve, Sabine Berteina-Raboin, Thomas M. Best, Eric Lespessailles, Hechmi Toumi
Summary: Osteoarthritis is a complex degenerative disease that disrupts joint homeostasis, leading to inflammation, cartilage degradation, and pain. Studies show that dietary components and natural products have potential for new therapeutic strategies. Current research is focused on natural-product-based treatments for osteoarthritis management.
Article
Orthopedics
H. Willcockson, H. Ozkan, L. Arbeeva, E. Mucahit, L. Musawwir, L. Longobardi
Summary: Deletion of Ccr2 in aggrecan-expressing cells reduces the initiation but not the progression of post-traumatic osteoarthritis.
OSTEOARTHRITIS AND CARTILAGE
(2022)
Article
Multidisciplinary Sciences
Julia Steinberg, Lorraine Southam, Theodoros Roumeliotis, Matthew J. Clark, Raveen L. Jayasuriya, Diane Swift, Karan M. Shah, Natalie C. Butterfield, Roger A. Brooks, Andrew W. McCaskie, J. H. Duncan Bassett, Graham R. Williams, Jyoti S. Choudhary, J. Mark Wilkinson, Eleftheria Zeggini
Summary: Researchers identified molecular trait loci and potential drug targets by analyzing cartilage and synovium from osteoarthritis patients.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Yusuke Fujiwara, Chenyang Ding, Yohei Sanada, Dilimulati Yimiti, Masakazu Ishikawa, Tomoyuki Nakasa, Naosuke Kamei, Kazunori Imaizumi, Martin K. Lotz, Takayuki Akimoto, Shigeru Miyaki, Nobuo Adachi
Summary: This study investigated the role of miR-23a/b clusters in osteoarthritis (OA). Through experiments on genetically modified mice, it was found that miR-23a/b clusters do not significantly contribute to the pathogenesis of OA.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Endocrinology & Metabolism
Shihua Zhang, Tingting Li, Yao Feng, Keping Zhang, Jun Zou, Xiquan Weng, Yu Yuan, Lan Zhang
Summary: Articular cartilage degeneration causes various joint diseases, with osteoarthritis being the most typical. Osteoarthritis is characterized by cartilage degeneration and persistent pain, impacting patient's quality of life and placing a burden on society. The occurrence and development of osteoarthritis is connected to subchondral bone microenvironment disorder. Exercise can improve the subchondral bone microenvironment, but the specific mechanism remains unclear.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Physiology
C. Huesa, L. Dunning, K. MacDougall, M. Fegen, A. Ortiz, K. McCulloch, S. McGrath, G. J. Litherland, A. Crilly, R. J. Van 't Hof, W. R. Ferrell, C. S. Goodyear, J. C. Lockhart
Summary: This study aimed to investigate the effect of moderate exercise on early joint pathology in a mouse model of osteoarthritis. The results showed that exercise protected against cartilage damage and offered temporary protection against osteosclerosis. Additionally, exercise modified the microarchitecture of the osteoarthritic leg. These findings support previous studies indicating the importance of exercise in improving bone health in osteoarthritis.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Biology
Hope C. Ball, Andrew L. Alejo, Trinity K. Samson, Amanda M. Alejo, Fayez F. Safadi
Summary: This review provides an updated overview of the epigenetic factors involved in osteoarthritis, discussing their impact on the onset and development of the disease, and exploring current therapeutic options targeting aberrant epigenetic regulation.
Article
Plant Sciences
Wei Peng, Wenjuan Zhang, Qici Wu, Shunyou Cai, Tingting Jia, Jiarui Sun, Zhichao Lin, Gulimiran Alitongbieke, Yixuan Chen, Yi Su, Jinmei Lin, Lisheng Cai, Yuqin Sun, Yutian Pan, Yu Xue
Summary: The study reveals that Glucosamine hydrochloride extracted from the cell wall of Agaricus bisporus plays a dose-dependent role in promoting cartilage and bone development, as well as caudal fin regeneration in adult fish, by regulating the expression of bone-related genes. Furthermore, GAH improves osteoporosis defects induced by high iron and promotes skeletal development and injury repair through bone morphogenetic protein signaling at the downstream of the receptor level.
JOURNAL OF NATURAL PRODUCTS
(2021)
Article
Cell Biology
Xiangfen Li, Xiaofang Zhu, Hongle Wu, Thomas E. Van Dyke, Xiaoyang Xu, Elise F. Morgan, Wenyu Fu, Chuanju Liu, Qisheng Tu, Dingming Huang, Jake Chen
Summary: Irisin is involved in cartilage development and osteoarthritis pathogenesis, with the potential to ameliorate OA progression by inhibiting inflammation and decreasing cartilage degradation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Chenhui Cai, Wenhui Hu, Tongwei Chu
Summary: This review summarizes the findings and observations in the field of iron overload-related osteoarthritis (OA), discussing the cellular and molecular mechanisms associated with iron overload and its negative impact on joint homeostasis. It also highlights the potential value of interrupting the pathological effects of iron overload for the development of improved therapeutics in the field of OA.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Juliette Bedrossiantz, Marina Bellot, Pol Dominguez-Garcia, Melissa Faria, Eva Prats, Cristian Gomez-Canela, Raul Lopez-Arnau, Elena Escubedo, Demetrio Raldua
Summary: This study successfully established a zebrafish model of METH-induced neurotoxicity, demonstrating a concentration- and time-dependent decrease in dopamine, norepinephrine, and serotonin levels in METH-exposed fish. Behavioral changes included hyperactivity, positive geotaxis, and negative scototaxis, as well as social isolation, while transcriptional changes indicated alterations in gene expression related to neuronal injury.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Endocrinology & Metabolism
Kristin Dietrich, Imke A. K. Fiedler, Anastasia Kurzyukova, Alejandra C. Lopez-Delgado, Lucy M. McGowan, Karina Geurtzen, Chrissy L. Hammond, Bjorn Busse, Franziska Knopf
Summary: Zebrafish, as teleosts, share common features with mammals in terms of skeletal elements and ossification mechanisms, but differences exist in bone morphology and function. They have high regenerative capacity and complex bone formation mechanisms, making them suitable for skeletal research.
JOURNAL OF BONE AND MINERAL RESEARCH
(2021)
Article
Cell Biology
Paco Lopez-Cuevas, Luke Deane, Yushi Yang, Chrissy L. Hammond, Erika Kague
Summary: Notochordal cells play a critical role in vertebral column patterning, and their transformation can disrupt bone development and intervertebral disc stability. Transformed notochord cells in zebrafish skeleton cause damage and activate a chronic wound response, indicating parallels between chordomas, wounds, IVD degeneration, and inflammation.
DISEASE MODELS & MECHANISMS
(2021)
Article
Cell & Tissue Engineering
E. A. Lawrence, J. Aggleton, J. van Loon, J. Godivier, R. Harniman, J. Pei, N. Nowlan, C. Hammond
Summary: The study found that exposure to hypergravity affects the material properties of cartilage and chondrocyte morphology in zebrafish, leading to changes in cartilage structure and function.
BONE & JOINT RESEARCH
(2021)
Article
Endocrinology & Metabolism
Jonathan H. Tobias, Emma L. Duncan, Erika Kague, Chrissy L. Hammond, Celia L. Gregson, Duncan Bassett, Graham R. Williams, Josine L. Min, Tom R. Gaunt, David Karasik, Claes Ohlsson, Fernando Rivadeneira, James R. Edwards, Fadil M. Hannan, John P. Kemp, Sophie J. Gilbert, Nerea Alonso, Neelam Hassan, Juliet E. Compston, Stuart H. Ralston
Summary: Recent advancements in identifying the role of sclerostin in sclerosteosis have led to the development of anti-sclerostin antibodies as a novel treatment for osteoporosis. Despite the discovery of numerous genetic signals associated with bone mass, the challenge remains in accurately pinpointing the responsible genes and identifying potential drug targets for further treatment.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Georgina L. K. McDonald, Mengdi Wang, Chrissy L. Hammond, Dylan J. M. Bergen
Summary: Osteoporosis and other conditions related to low bone density are becoming more prevalent as the population ages and glucocorticoid use increases. Research suggests that targeting genes associated with high bone mass, such as SMAD9, could offer potential for future osteoanabolic therapies.
Article
Cell Biology
Nicola L. Stevenson, Dylan J. M. Bergen, Yinhui Lu, M. Esther Prada-Sanchez, Karl E. Kadler, Chrissy L. Hammond, David J. Stephens
Summary: Knockout of the golgin giantin leads to skeletal and craniofacial defects due to changes in glycosylation and extracellular matrix deposition. Our study reveals a conserved role for giantin in collagen biosynthesis and extracellular matrix assembly, as well as a giantin-dependent pathway for intracellular procollagen processing.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Lucy Brunt, Gediminas Greicius, Sally Rogers, Benjamin D. Evans, David M. Virshup, Kyle C. A. Wedgwood, Steffen Scholpp
Summary: Vangl2 regulates Wnt ligand distribution by controlling cytoneme behavior, enhancing paracrine Wnt/β-catenin signaling. This study reveals the crucial role of Vangl2 in intercellular Wnt signal transduction through cytonemes.
NATURE COMMUNICATIONS
(2021)
Review
Hematology
Stephanie Pellegrin, Charlotte E. Severn, Ashley M. Toye
Summary: This review discusses recent progress in the erythroid culture field as well as opportunities for further scaling up of manufactured RBC production for transfusion practice.
Article
Biology
Dylan J. M. Bergen, Qiao Tong, Ankit Shukla, Elis Newham, Jan Zethof, Mischa Lundberg, Rebecca Ryan, Scott E. Youlten, Monika Frysz, Peter I. Croucher, Gert Flik, Rebecca J. Richardson, John P. Kemp, Chrissy L. Hammond, Juriaan R. Metz
Summary: Dermal scales possess a strong osteogenic expression profile similar to bone, enriched in genes favoring collagen matrix growth. Despite differences in developmental processes between scale and endoskeleton, zebrafish scales express a subset of evolutionarily conserved genes relevant to human skeletal diseases.
Article
Biochemical Research Methods
Yushi Yang, Francesco Turci, Erika Kague, Chrissy L. Hammond, John Russo, C. Patrick Royall
Summary: In this study, the collective behavior of 50 zebrafish was investigated in a three-dimensional manner. The researchers found that the fish exhibited a transition from ordered to random behavior when they adapted to new environmental conditions. By quantifying the spatial and temporal correlation functions, the researchers identified two length scales that captured the essence of these behavioral changes. The ratio of these length scales was found to correlate with the polarization of collective motion, which was explained using a simplified model of self-propelled particles with alignment interactions.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Anatomy & Morphology
Josepha Godivier, Elizabeth A. Lawrence, Mengdi Wang, Chrissy L. Hammond, Niamh C. Nowlan
Summary: This study quantified key cellular dynamics involved in growth and morphogenesis of the zebrafish jaw joint and used computational simulations to predict joint development, highlighting the dominant influence of growth anisotropy on joint growth and morphogenesis. The findings contribute to understanding the pathogenesis of developmental joint disorders.
JOURNAL OF ANATOMY
(2022)
Article
Chemistry, Multidisciplinary
Paco Lopez-Cuevas, Can Xu, Charlotte E. Severn, Tiah C. L. Oates, Stephen J. Cross, Ashley M. Toye, Stephen Mann, Paul Martin
Summary: This study demonstrates that silencing or knocking out a specific immune cell-expressed miRNA, microRNA-223 (miR223), can reduce cancer progression. By using miniature artificial protocells (PCs) to deliver anti-inflammatory miRNAs, the interactions between immune cells and cancer cells are altered, resulting in reduced tumor burden.
Article
Cell & Tissue Engineering
Jeremie Zappia, Qiao Tong, Renee Van der Cruyssen, Frederique M. F. Cornelis, Cecile Lambert, Tiago Pinto Coelho, Juliane Grisart, Erika Kague, Rik J. Lories, Marc Muller, Dirk Elewaut, Chrissy L. Hammond, Christelle Sanchez, Yves Henrotin
Summary: Abnormal subchondral bone remodeling leading to sclerosis is a main feature of osteoarthritis. Osteomodulin (OMD), a proteoglycan, is associated with the sclerotic phenotype. This study found that OMD binds to RANKL and inhibits osteoclastogenesis, thus regulating bone remodeling and contributing to bone and cartilage homeostasis. Targeting OMD may be a promising personalized approach for osteoarthritis.
Article
Cell & Tissue Engineering
Erika Kague, Francesco Turci, Elis Newman, Yushi Yang, Kate Robson Brown, Mona S. Aglan, Ghada A. Otaify, Samia A. Temtamy, Victor L. Ruiz-Perez, Stephen Cross, C. Patrick Royall, P. Eckhard Witten, Chrissy L. Hammond
Summary: Back pain is a common condition with high social impact, largely attributed to intervertebral disc disease (IVDD). The role of bone mineral density (BMD) in IVDD remains controversial, with genetic components potentially influencing the disease. Using an aging zebrafish model, it was found that increased BMD in aging can lead to changes resembling osteoporosis, and mutations in sp7 and cathepsin K affecting BMD levels also impacted the development of IVDD.
Article
Endocrinology & Metabolism
Lucy M. McGowan, Erika Kague, Alistair Vorster, Elis Newham, Stephen Cross, Chrissy L. Hammond
Summary: In this study, CRISPR-Cas9 was used to generate mutant zebrafish lacking Wnt16 to investigate its effect on bone. The results suggest that Wnt16 plays a crucial role in bone health and repair by modulating canonical Wnt activity to facilitate osteoblast differentiation and bone matrix deposition.