Article
Biotechnology & Applied Microbiology
Gustavo A. Higuera, Tiago Ramos, Antonio Gloria, Luigi Ambrosio, Andrea Di Luca, Nicholas Pechkov, Joost R. de Wijn, Clemens A. van Blitterswijk, Lorenzo Moroni
Summary: Additively manufactured scaffolds have custom-shaped structures with completely interconnected pore networks, and the surface properties of scaffolds greatly impact the success of engineered tissue structures. By combining microspheres with additive manufacturing technologies, 3D scaffolds with adjustable surface roughness can enhance cell adhesion and metabolic activity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Chengqi Lyu, Chi Cheng, YuShi He, Ling Qiu, Zijun He, Derong Zou, Dan Li, Jiayu Lu
Summary: Porous scaffolds are commonly used for cartilage tissue regeneration, but understanding the impact of their hierarchical structure on the regeneration process can be challenging. In this study, a graphene hydrogel with stable and adjustable structure was used as a model scaffold to investigate the effect of porous structure on matrix remodeling and chondrocyte ingrowth. The results showed that the graphene scaffold with an open pore structure accelerated and balanced cartilage remodeling. Furthermore, this enhanced remodeling selectively promoted the expression of collagen type II fibrils, providing new insights into scaffold design for cartilage repair.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Engineering, Biomedical
Minglei Yang, Jiafei Chen, Yi Chen, Weikang Lin, Hai Tang, Ziwen Fan, Long Wang, Yunlang She, Feng Jin, Lei Zhang, Weiyan Sun, Chang Chen
Summary: A scaffold-free modular strategy for tracheal engineering is developed, which constructs a complex tracheal substitute by laminating a cartilage sheet and a temperature-sensitive epithelium sheet. This substitute exhibits native-like structure and function, showing great clinical potential for tracheal reconstruction.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Mathematics
Inigo Calderon-Uriszar-Aldaca, Sergio Perez, Ravi Sinha, Maria Camara-Torres, Sara Villanueva, Carlos Mota, Alessandro Patelli, Amaia Matanza, Lorenzo Moroni, Alberto Sanchez
Summary: Finite element modeling was used to simulate AM scaffolds for bone tissue regeneration, with experimental validation using polymer and nano-filler composites. The simulations overestimated scaffold performance due to lack of printing accuracy, highlighting the importance of calibration for expected scenarios.
Article
Nanoscience & Nanotechnology
Zhiyao Liao, Liwei Fu, Pinxue Li, Jiang Wu, Xun Yuan, Chao Ning, Zhengang Ding, Xiang Sui, Shuyun Liu, Quanyi Guo
Summary: A bioactive multifunctional scaffold was developed using Mg2+ and aptamer Apt19S to promote articular cartilage regeneration and early inflammatory regulation, offering a promising strategy for AC defect treatment.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Polymer Science
Eva Petrovova, Marek Tomco, Katarina Holovska, Jan Danko, Lenka Kresakova, Katarina Vdoviakova, Veronika Simaiova, Filip Kolvek, Petra Hornakova, Teodor Toth, Jozef Zivcak, Peter Gal, David Sedmera, Lenka Luptakova, Lubomir Medvecky
Summary: Biopolymer composites provide an optimal environment for the regeneration of articular cartilage defects, with potential for clinical testing in the orthopedic field. The use of acellular polyhydroxybutyrate/chitosan implants in sheep models showed promising results, with hyaline-like cartilage formation observed in most treated animals.
Article
Biophysics
Chuan Guo, Zhenxing Cao, Yan Peng, Rui Wu, Hu Xu, Zhaoyang Yuan, Hui Xiong, Yu Wang, Ye Wu, Weilong Li, Qingquan Kong, Yi Wang, Jinrong Wu
Summary: Inspired by the microstructures of subchondral bone, a cell and cytokine free hydrogel scaffold for cartilage regeneration was developed, showing promising prospects in cartilage regeneration and various bioremediation applications.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Engineering, Multidisciplinary
Hongyi Chen, Giovanni Gonnella, Jie Huang, Lucy Di-Silvio
Summary: Treatments for osteochondral defects are currently palliative, but the increasing prevalence of this pathology necessitates the search for alternative treatment modalities. Tissue-engineered strategies for regenerating the cartilage-bone interface have been explored, with cell-laden hydrogel scaffolds being a common approach. However, these scaffolds often lack homogenous cell dispersion and patient specificity. In this study, a biphasic 3D bioprinted composite scaffold was developed using alginate-gelatin hydrogel and polycaprolactone, showing promise as an interfacial material between cartilage and bone.
Article
Engineering, Biomedical
Sinan Wang, Bin Luo, Baoshuai Bai, Qianyi Wang, Hongying Chen, Xiaoyan Tan, Zhengya Tang, Sisi Shen, Hengxing Zhou, Zhengwei You, Guangdong Zhou, Dong Lei
Summary: Tissue engineering is a promising approach for cartilage regeneration, and the modification and drug loading of poly(glycerol sebacate) (PGS) scaffolds pose a key challenge. In this study, a new strategy of super swelling-absorption and cross-linked networks locking was used to create a 3D printed PGS-CS/Gel scaffold based on FDA-approved PGS, gelatin, and chondroitin sulfate. The PGS-CS/Gel scaffold exhibited desirable properties and successfully repaired cartilage in a rabbit model.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Surgery
Jinping Ding, Shiwei Bao, Wenjiang Qian, Hongyi Zhao
Summary: The chondrocyte sheet technique successfully regenerated mature and stable engineered cartilages in a pig model, avoiding the impact of inflammatory reactions.
PLASTIC AND RECONSTRUCTIVE SURGERY
(2021)
Article
Materials Science, Multidisciplinary
Minglei Yang, Weiyan Sun, Long Wang, Hai Tang, Xiang Xu, Liangwei Yang, Junjun Ni, Kuoen Zheng, Xu Jiang, Weiwen Xu, Guofang Zhao, Yunlang She, Lei Zhang, Dong Xie, Chang Chen
Summary: This study successfully promoted tracheal cartilage regeneration and tracheal defect repair using the Cur/PCL scaffold, demonstrating significant anti-inflammatory effects and improved survival rates in experimental rabbits.
MATERIALS & DESIGN
(2022)
Review
Nanoscience & Nanotechnology
Zhen Yang, Tianyuan Zhao, Cangjian Gao, Fuyang Cao, Hao Li, Zhiyao Liao, Liwei Fu, Pinxue Li, Wei Chen, Zhiqiang Sun, Shuangpeng Jiang, Zhuang Tian, Guangzhao Tian, Kangkang Zha, Tingting Pan, Xu Li, Xiang Sui, Zhiguo Yuan, Shuyun Liu, Quanyi Guo
Summary: This study developed a 3D-bioprinted difunctional scaffold based on aptamer HM69-mediated MSC-specific recruitment and growth factor-enhanced cell chondrogenesis, which greatly improved cartilage repair in rabbit full-thickness defects. The study demonstrated that 3D bioprinting of difunctional scaffolds could be a promising strategy for in situ AC regeneration.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Engineering, Biomedical
Shuangpeng Jiang, Guangzhao Tian, Zhen Yang, Xiang Gao, Fuxin Wang, Juntan Li, Zhuang Tian, Bo Huang, Fu Wei, Xinyu Sang, Liuqi Shao, Jian Zhou, Zhenyong Wang, Shuyun Liu, Xiang Sui, Quanyi Guo, Weimin Guo, Xu Li
Summary: This study confirms that hWJMSC-Exos can enhance the effect of the ACECM scaffold and promote osteochondral regeneration. The promoting effect may be related to the polarization of macrophages and inhibition of inflammatory response by hWJMSC-Exos. Additionally, hWJMSC-Exos contain miRNAs that can promote hyaline cartilage regeneration.
BIOACTIVE MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Feng Lin, Yaping Zhuang, Lei Xiang, Tingjun Ye, Zhen Wang, Longxi Wu, Yupu Liu, Lianfu Deng, Wenguo Cui
Summary: In this study, a heat transfer microneedle system called ST-needle was constructed, which used dynamic spiral Mosaic technology and a dual delivery system for heat energy and biological factors. The ST-needle system was found to effectively inhibit chondrocyte apoptosis. This research is of great significance for lesion repair.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Engineering, Biomedical
Wei Chen, Yongsheng Li, Yuting Huang, Yao Dai, Tingfei Xi, Zheng Zhou, Hairong Liu
Summary: The study indicates that quercetin-modified PHBV fibrous scaffold has the potential to promote cartilage regeneration in tissue engineering. By regulating related genes, it can inhibit chondrocyte apoptosis and reduce oxidative stress.
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
(2021)
Review
Engineering, Biomedical
Arwa Daghrery, Isaac J. de Souza Araujo, Miguel Castilho, Jos Malda, Marco C. Bottino
Summary: For almost three decades, tissue engineering strategies have been used to create effective therapies for dental, oral, and craniofacial regenerative medicine by treating permanent deformities caused by debilitating health conditions. Additive manufacturing techniques, such as melt electrowriting (MEW), enable the creation of personalized scaffolds that can replicate native tissue characteristics using 3D printing technology. Recent advancements have shown that combining MEW with other biofabrication techniques can overcome some limitations and offer promising opportunities for tissue regeneration.
ACTA BIOMATERIALIA
(2023)
Review
Engineering, Biomedical
Leanne de Silva, Paulina N. Bernal, A. J. W. Rosenberg, Jos Malda, Riccardo Levato, Debby Gawlitta
Summary: The development of tissue engineering strategies for treatment of large bone defects is crucial, and creating a vascular network is vital for tissue engineered bone constructs.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Multidisciplinary
Bram G. Soliman, Alessia Longoni, Mian Wang, Wanlu Li, Paulina N. Bernal, Alessandro Cianciosi, Gabriella C. J. Lindberg, Jos Malda, Juergen Groll, Tomasz Jungst, Riccardo Levato, Jelena Rnjak-Kovacina, Tim B. F. Woodfield, Yu Shrike Zhang, Khoon S. Lim
Summary: Sacrificial printing involves using 3D-printed sacrificial ink to create open-channels in hydrogel constructs. Traditional sacrificial inks lack the ability to mimic tissue development dynamics. To overcome this, a new class of sacrificial inks was developed with tailorable and programmable delayed dissolution profiles. These inks showed compatibility with various biofabrication technologies and allowed precise control over the introduction of architectural features into cell-laden hydrogel constructs.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Orthopedics
Shaghayegh Basatvat, Frances C. Bach, Marcos N. Barcellona, Abbie L. Binch, Conor T. Buckley, Brian Bueno, Nadeen O. Chahine, Ana Chee, Laura B. Creemers, Stefan Dudli, Bailey Fearing, Stephen J. Ferguson, Jennifer Gansau, Benjamin Gantenbein, Rahul Gawri, Juliane D. Glaeser, Sibylle Grad, Julien Guerrero, Lisbet Haglund, Paula A. Hernandez, Judith A. Hoyland, Charles Huang, James C. Iatridis, Svenja Illien-Junger, Liufang Jing, Petra Kraus, Lisanne T. Laagland, Gernot Lang, Victor Leung, Zhen Li, Thomas Lufkin, Josette C. van Maanen, Emily E. McDonnell, Chris J. Panebianco, Steven M. Presciutti, Sanjna Rao, Stephen M. Richardson, Sarah Romereim, Tara C. Schmitz, Jordy Schol, Lori Setton, Dmitriy Sheyn, Joseph W. Snuggs, Y. Sun, Xiaohong Tan, Marianna A. Tryfonidou, Nam Vo, Dong Wang, Brandon Williams, Rebecca Williams, S. Tim Yoon, Christine L. Le Maitre
Summary: This study aims to standardize the extraction and expansion techniques for nucleus pulposus (NP) cells, in order to reduce variability between laboratories and improve comparability of results. The most commonly used methods for NP cell extraction, expansion, and re-differentiation were identified through a global survey. Recommended protocols for NP cell culture in different species were provided to support harmonization and cross-lab comparisons.
Article
Engineering, Biomedical
Madison Jade Ainsworth, Nino Chirico, Mylene de Ruijter, Andrei Hrynevich, Inge Dokter, Joost P. G. Sluijter, Jos Malda, Alain van Mil, Miguel Castilho
Summary: In order to develop thick engineered cardiac tissue construct with a pre-vascularization pathway, a combination of extrusion-based bioprinting and melt electrowriting techniques was investigated. Gelatin methacryloyl (GelMA) was used as a base hydrogel and collagen, Matrigel, and fibrinogen were utilized as interpenetrating polymers to support myocardial functionality. An anatomically inspired vascular pathway was implemented in a dual extrusion-based bioprinting setup. The optimized bioinks effectively filled the microfiber mesh and maintained cell viability, and distinct cell populations were visualized in their respective patterns up to D14.
Article
Engineering, Biomedical
Angelique Van Ombergen, Franziska Chalupa-Gantner, Parth Chansoria, Bianca Maria Colosimo, Marco Costantini, Marco Domingos, Alexandre Dufour, Carmelo De Maria, Juergen Groll, Tomasz Jungst, Riccardo Levato, Jos Malda, Alessandro Margarita, Christophe Marquette, Aleksandr Ovsianikov, Emma Petiot, Sophia Read, Leonardo Surdo, Wojciech Swieszkowski, Giovanni Vozzi, Johannes Windisch, Marcy Zenobi-Wong, Michael Gelinsky
Summary: 3D bioprinting has made significant progress in recent years, allowing for the creation of both simple and complex tissue models. The international space agencies recognize the potential of this technology for manufacturing cell and tissue models for space research, particularly in studying the effects of microgravity and cosmic radiation on human tissues. Additionally, bioprinting can produce tissue grafts for clinical applications, which can support medical treatment for astronauts in long-term space missions. The article discusses the opportunities and challenges of using bioprinters in space, particularly in microgravity. While certain steps may be challenging in microgravity, such as handling liquids, this environment can also help overcome issues like cell sedimentation in low-viscosity bioinks. The article hopes to inspire more researchers to explore this field, especially with the availability of bioprinting opportunities at the International Space Station (ISS) in the near future.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Marc Falandt, Paulina Nunez Bernal, Oksana Dudaryeva, Sammy Florczak, Gabriel Grossbacher, Matthias Schweiger, Alessia Longoni, Coralie Greant, Marisa Assuncao, Olaf Nijssen, Sandra van Vlierberghe, Jos Malda, Tina Vermonden, Riccardo Levato
Summary: This study presents a new approach using light-based volumetric printing to spatially pattern any biomolecule of interest on custom-designed hydrogels. By developing a gelatin norbornene resin with tunable mechanical properties, the resin can be quickly printed at high resolution. Thiol-ene click chemistry allows for on-demand photografting of thiolated compounds postprinting, enabling the precise biofunctionalization and modification of (bio)printed constructs to guide cell behavior and create bioactive cue gradients. This technology also opens possibilities for 4D printing to mimic the dynamic changes in morphogen presentation in biological tissues.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Engineering, Biomedical
Jonathan H. Galarraga, Hannah M. Zlotnick, Ryan C. Locke, Sachin Gupta, Natalie L. Fogarty, Kendall M. Masada, Brendan D. Stoeckl, Lorielle Laforest, Miguel Castilho, Jos Malda, Riccardo Levato, James L. Carey, Robert L. Mauck, Jason A. Burdick
Summary: Soft norbornene-modified hyaluronic acid (NorHA) hydrogels were reinforced with polycaprolactone using melt electrowriting (MEW) to fabricate composite scaffolds. These scaffolds supported chondrogenesis and cartilage formation of encapsulated porcine mesenchymal stromal cells (pMSC) and exhibited suitable mechanical properties. Fixation of the composites with biodegradable pins or fibrin glue ensured implant retention, but the use of pins resulted in inferior cartilage repair compared to the use of glue.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Orthopedics
Jasper G. Steverink, Floris R. van Tol, Suzanne Bruins, Andre J. Smorenburg, Marianna A. Tryfonidou, Bas J. Oosterman, Marijke R. van Dijk, Jos Malda, Jorrit-Jan Verlaan
Summary: This study investigates the local toxic effects of highly concentrated (5%) bupivacaine compared to clinically used concentrations in vivo following skeletal surgery. The results show that bupivacaine concentration is negatively correlated with local tissue damage, and the mode of administration and implantation site have no significant effect on tissue damage.
JOURNAL OF EXPERIMENTAL ORTHOPAEDICS
(2023)
Article
Engineering, Biomedical
Eng Kuan Moo, Mohammadhossein Ebrahimi, Andrei Hrynevich, Mylene de Ruijter, Miguel Castilho, Jos Malda, Rami K. Korhonen
Summary: The melt-electrowriting (MEW) technique allows the printing of structured fibrous mesh with small fiber diameter, which enhances the mechanical stiffness of soft hydrogels. The reinforcing mechanism of MEW meshes is not well understood, but it may involve load-induced fluid pressurization. In this study, the reinforcing effect of MEW meshes in different hydrogels was examined, and it was found that MEW meshes only enhanced fluid pressurization in gelatin methacryloyl (GelMA) hydrogel.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2023)
Article
Materials Science, Biomaterials
Gerardo Cedillo-Servin, Ana Filipa Louro, Beatriz Gamelas, Ana Meliciano, Anne Zijl, Paula M. Alves, Jos Malda, Margarida Serra, Miguel Castilho
Summary: Microfiber-reinforced GelMA hydrogels can serve as sustained delivery platforms for human induced pluripotent stem cell (hiPSC)-derived extracellular vesicles (EV). The release of EV can be delayed and the extent of release can be reduced by reinforcing the hydrogels with highly porous microfiber meshes.
BIOMATERIALS ADVANCES
(2023)
Article
Orthopedics
Andrea Schwab, Marinus A. Wesdorp, Jietao Xu, Florencia Abinzano, Claudia Loebel, Marc Falandt, Riccardo Levato, David Eglin, Roberto Narcisi, Martin J. Stoddart, Jos Malda, Jason A. Burdick, Matteo D'Este, Gerjo J. V. M. Van Osch
Summary: This study investigates the influence of various physicochemical properties of hydrogels on cell migration and related tissue formation using in vitro, ex vivo and in vivo models.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2023)
Article
Multidisciplinary Sciences
Francesca Cialdai, Austin M. Brown, Cory W. Baumann, Debora Angeloni, Sarah Baatout, Alexandra Benchoua, Juergen Bereiter-Hahn, Daniele Bottai, Judith-Irina Buchheim, Marco Calvaruso, Eugenie Carnero-Diaz, Sara Castiglioni, Duccio Cavalieri, Gabriele Ceccarelli, Alexander Chouker, Gianni Ciofani, Giuseppe Coppola, Gabriella Cusella, Andrea Degl'Innocenti, Jean-Francois Desaphy, Jean-Pol Frippiat, Michael Gelinsky, Giada Genchi, Maria Grano, Daniela Grimm, Alain Guignandon, Christiane Hahn, Jason Hatton, Raul Herranz, Christine E. Hellweg, Carlo Saverio Iorio, Thodoris Karapantsios, Jack van Loon, Matteo Lulli, Jeanette Maier, Jos Malda, Emina Mamaca, Lucia Morbidelli, Angelique van Ombergen, Andreas Osterman, Aleksandr Ovsianikov, Francesco Pampaloni, Elizabeth Pavezlorie, Veronica Pereda-Campos, Cyrille Przybyla, Christopher Puhl, Petra Rettberg, Chiara Risaliti, Angela Maria Rizzo, Kate Robson-Brown, Leonardo Rossi, Giorgio Russo, Alessandra Salvetti, Daniela Santucci, Matthias Sperl, Felice Strollo, Kevin Tabury, Sara Tavella, Christiane Thielemann, Ronnie Willaert, Nathaniel J. Szewczyk, Monica Monici
Summary: This white paper presents the indications and recommendations of the SciSpacE Science Community on filling the gaps of knowledge regarding the effects of gravity alterations on animal and human systems at a cellular and tissue level. Despite previous studies, a comprehensive integrated model of the changes occurring at different system and functional levels is still lacking, making it difficult to predict the long-term consequences of human adaptation to the space environment and implement effective medical support plans.
Article
Biotechnology & Applied Microbiology
Mylene de Ruijter, Paweena Diloksumpan, Inge Dokter, Harold Brommer, Ineke H. Smit, Riccardo Levato, P. Rene van Weeren, Miguel Castilho, Jos Malda
Summary: A bioprinted osteochondral implant, based on gelatin methacryloyl cartilage phase and reinforced with precisely patterned melt electrowritten polycaprolactone fibers, can provide long-term mechanically stable neo-tissue in an animal model. Mechanical stability is more important than the presence of cells and pre-cultured extracellular matrix for the successful survival of the implant.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Engineering, Biomedical
Shima Tavakoli, Austin Evans, Oommen P. Oommen, Laura Creemers, Jharna Barman Nandi, Jons Hilborn, Oommen P. Varghese
Summary: Labeling and visualization of extracellular matrix (ECM) components secreted by cells and living tissues is important for biomedical researchers. The use of bioorthogonal chemistry approaches allows for the specific visualization and labeling of ECM constituents without interfering with other chemical or biological events. This review summarizes recent advancements in using biorthogonal chemistry for metabolic labeling and visualization of ECM proteins and glycosaminoglycans, discusses challenges and limitations, and suggests potential solutions for improvement.
MATERIALS TODAY BIO
(2023)
