Journal
TOXICOLOGY
Volume 335, Issue -, Pages 27-34Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.tox.2015.07.001
Keywords
ATR-FTIR spectroscopy; Benzo[a]pyrene; DNA damage; MCF-7 cells; Nucleus isolation; Polycyclic aromatic hydrocarbon
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Funding
- Natural Environment Research Council [ceh010010] Funding Source: researchfish
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Exposure to chemicals such as benzo[alpyrene (B[a]P) can generate intracellular toxic mechanisms. Fourier-transform infrared (FTIR) spectroscopy is a novel approach that allows the non-destructive analysis of underlying chemical bond alterations in patho-physiological processes. This study set out to examine whether B[a]P-induced whole cell alterations could be distinguished from effects on nuclei of exposed cells. Using attenuated total reflection FTIR (ATR FTIR) spectroscopy, alterations in nuclei isolated from B[a]P-treated MCF-7 cells concentrated either in G(0)/G(1)- or S-phase were observed. B[a113induced effects in whole-cells included alterations to lipids, DNA and protein spectral regions. Absorbance areas for protein and DNA/RNA regions in B[a]P-treated whole cells differed significantly (P<0.0001) from vehicle controls and these observations correlated with alterations noted in isolated nuclei. Our findings provide evidence that FTIR spectroscopy has the ability to identify specific chemical-induced alterations. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
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