Article
Environmental Sciences
Yipeng Sun, Zhaowen Shi, Yuhua Lin, Mengzhe Zhang, Jinjin Liu, Linyun Zhu, Qingge Chen, Junjie Bi, Shanqun Li, Zhenhua Ni, Xiongbiao Wang
Summary: This study demonstrated that BaP stimulation increased MUC5AC expression through the activation of the AhR/mitochondrial ROS/ERK pathway. Inhibition of ERK or knockdown of ERK suppressed BaP-induced MUC5AC expression. BaP-induced MUC5AC overexpression was mediated by AhR translocation, mitochondrial ROS production, and ERK activation.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Review
Biochemistry & Molecular Biology
Marco Gargaro, Giulia Scalisi, Giorgia Manni, Giada Mondanelli, Ursula Grohmann, Francesca Fallarino
Summary: The aryl-hydrocarbon receptor (AhR) is a ligand-activated transcription factor that mediates various cellular responses and has attracted significant attention in the field of immunology in recent years. In addition to its role in toxicology, AhR also plays a crucial role in normal cell physiology, mediating toxic or physiologic effects in response to multiple exogenous and endogenous molecules. Various factors, such as the nature of the ligand, its metabolism by AhR-induced enzymes, tissue microenvironment, and the presence of coregulators or specific transcription factors, contribute to the outcome of gene transcriptional regulation by AhR.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Gastroenterology & Hepatology
Marta Vuerich, Rasika Harshe, Luiza Abrahao Frank, Samiran Mukherjee, Barbora Gromova, Eva Csizmadia, Imad A. M. Nasser, Yun Ma, Alan Bonder, Vilas Patwardhan, Simon C. Robson, Maria Serena Longhi
Summary: In patients with autoimmune hepatitis, dysfunction of the aryl hydrocarbon receptor pathway, resulting from aberrant inhibition or non-canonical activation, impairs Treg- and Th17 cell-induced upregulation of CD39, a key ectoenzyme for immuno-regulation. Blockade of excessive inhibition or non-canonical activation of the aryl hydrocarbon receptor pathway might represent a novel therapeutic strategy to control inflammation while restoring immune balance in autoimmune hepatitis.
JOURNAL OF HEPATOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Maciej Jaskiewicz, Adrianna Moszynska, Jaroslaw Kroliczewski, Aleksandra Cabaj, Sylwia Bartoszewska, Agata Charzynska, Magda Gebert, Michal Dabrowski, James F. Collawn, Rafal Bartoszewski
Summary: HIF is a transcription factor that activates the adaptive hypoxic response during low oxygen levels. HIF-1 and HIF-2 mediate this response in a sequential manner, but their transcriptional profiles differ and the transition between them is not well understood.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Shaoying Gong, Jiachen Zheng, Junjie Zhang, Jianchun Han
Summary: This study investigated the effect of benzo[a]pyrene (B[a]P) on the function of intestinal epithelial barrier (IEB) and the protective effect of arabinogalactan (AG) against B[a]P-induced IEB dysfunction using a Caco-2 cell monolayer model. B[a]P was found to damage the IEB integrity by inducing cell cytotoxicity, oxidative stress, pro-inflammatory cytokine secretion, down-regulation of tight junction proteins, and activation of the AhR/MAPK signaling pathway. However, AG mitigated B[a]P-induced IEB dysfunction by inhibiting oxidative stress and pro-inflammatory factor secretion.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Medicine, Research & Experimental
Tripti Singh, Pallabi Banerjee, Uditi, Sarita Kumari, Anita Chopra, Nagendra Singh, Imteyaz Qamar
Summary: Regucalcin regulates intracellular Ca2+ homeostasis and intracellular signaling pathways. Its expression in hypoxia is upregulated by HIF-1α through direct binding to the HRE binding motifs within the Regucalcin promoter.
Review
Biochemistry & Molecular Biology
Thuy-Hang Nguyen, Stephanie Conotte, Alexandra Belayew, Anne-Emilie Decleves, Alexandre Legrand, Alexandra Tassin
Summary: Muscular dystrophies are genetic degenerative muscle disorders characterized by muscle wasting, often accompanied by respiratory impairments and hypoxemia. Hypoxic stress activates compensatory mechanisms, with HIF-1α playing a key role. Alterations in HIF-1α in muscle may impact the pathophysiology of muscular dystrophies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Xiao-Yi Chen, Qian-Long Wang, Wei-Xue Huang, Long-Wei Li, Lan-Cong Liu, Yi Yang, You-Jiao Wu, Shan-Shan Song, Hao Ma, Hua Zhou, Pei Luo
Summary: This study aimed to analyze and evaluate the dynamic changes in HIF-1 alpha concentration in serum exosomes during bacterial peritonitis. A new colorimetric method to quantitate these changes was established. The results showed that the serum exosomal HIF-1 alpha levels increased in the early stage of bacterial peritonitis, reached a peak at 24-48 hours, and then gradually decreased. Intervention treatment reduced the abdominal infection extent, HIF-1 alpha concentration in serum exosomes, and the serum pro-inflammatory factors levels. These findings suggest that serum exosomal HIF-1 alpha can be used as a biomarker for predicting and monitoring the pathological process of bacterial peritonitis.
Article
Plant Sciences
Bo-Han Wang, Ling-Ling Tang, Xian-Hong Sun, Qian Zhang, Chun-Yang Liu, Xiao-Na Zhang, Ke-Yao Yu, Ying Yang, Jun Hu, Xiao-Lu Shi, Yue Wang, Li Liu
Summary: QFXBF can inhibit airway mucus secretion exacerbated by air pollutants, possibly by suppressing the AhR/ROS/ERK signaling pathway.
JOURNAL OF ETHNOPHARMACOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Jangsoon Kim, See-Hyoung Park, Seyoung Yang, Sae Woong Oh, Kitae Kwon, Se Jung Park, Eunbi Yu, Hyeyoun Kim, Jung Yoen Park, Seoyoung Choi, Seoyeon Yang, Minkyung Song, Jae Youl Cho, Jongsung Lee
Summary: Maclurin exhibited antagonistic activity against B[a]P effects by inhibiting AHR signaling and activating Nrf2-mediated signaling, suggesting its potential in protecting from B[a]P-containing pollutants. Additionally, its antioxidant property was demonstrated through ROS inhibition and activation of ARE signaling.
Article
Biochemistry & Molecular Biology
Yu-Kun Jie, Jian-Jun Jiang, Hong-Ling Ma, Chang -Hong Cheng, Guang-Xin Liu, Si-Gang Fan, Juan Feng, Zhi-Xun Guo
Summary: In this study, a novel PHD2 gene, SpPHD2, was cloned and identified in the mud crab Scylla paramamosain. The mRNA expression of SpPHD2 was widely distributed across all examined tissues, and the protein was mainly localized in the cytoplasm. Silencing SpPHD2 in vivo upregulated SpHIF-1 alpha and downstream genes, while overexpression of SpPHD2 in vitro dose-dependently reduced SpHIF-1 alpha transcriptional activity, indicating the crucial role of SpPHD2 in regulating SpHIF-1 alpha.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Genetics & Heredity
Sarah R. Poll, Renan Martin, Elizabeth Wohler, Elizabeth S. Partan, Elizabeth Walek, Shaima Salman, Daniel Groepper, Lisa Kratz, Mirlene Cernach, Reynaldo Jesus-Garcia, Chad Haldeman-Englert, Yoon Jae Choi, Carol D. Morris, Bernard Cohen, Julie Hoover-Fong, David Valle, Gregg L. Semenza, Nara L. M. Sobreira
Summary: Ollier disease and Maffucci Syndrome are rare disorders characterized by multiple enchondromas and have a high risk of developing malignancies. Variants in HIF1A, VHL, and IDH1 genes have been found to be significantly associated with these disorders. Functional studies are needed to confirm the role of these genetic variants in the development of phenotypic abnormalities in individuals with Ollier disease and Maffucci Syndrome.
Review
Cell Biology
Minghui Zhang, Yu Zhang, Yubo Ding, Jialu Huang, Jingwei Yao, Zhuoyi Xie, Yufan Lv, Jianhong Zuo
Summary: This review examines the regulatory roles of HIF-1 alpha and lncRNAs in the EMT process of tumor cells and their relationship with tumorigenesis, and summarizes current drugs that inhibit tumor progression by modulating HIF-1 alpha.
Article
Engineering, Biomedical
Ming-Xuan Liu, Li Xu, Yu-Ting Cai, Ruo-Jia Wang, Ying-Ying Gu, Yan-Chao Liu, Yu-Jin Zou, Yong-Mei Zhao, Jing Chen, Xiao-Ling Zhang
Summary: This study established a safe and effective method to downregulate the expression of HIF-1 alpha in hepatic stellate cells (HSCs) and treat liver fibrosis. By delivering HIF-1 alpha small interfering RNA (HIF-1 alpha-siRNA) via VA-PEG-modified CNs-based nanosheets encapsulated with GQDs, the expression of HIF-1 alpha can be decreased. Additionally, the nanosheets can trigger water splitting under near-infrared (NIR) irradiation, improving the hypoxic liver fibrosis site and achieving better therapeutic effects.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Kenta Kaihara, Shuji Nakagawa, Yuji Arai, Hiroaki Inoue, Shinji Tsuchida, Yuta Fujii, Yoichiro Kamada, Tsunao Kishida, Osam Mazda, Kenji Takahashi
Summary: The study found that hypoxia can suppress the development of rheumatoid arthritis by controlling HIF-1 alpha expression, delaying joint swelling and reducing joint destruction. Deferoxamine-mediated activation of HIF-1 alpha can counteract the inhibitory effect of sustained hypoxia on pro-inflammatory cytokines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Toxicology
Cheng-Hui Lin, Po-Lin Liao, George Hsiao, Ching-Hao Li, Shih-Hsuan Huang, Chi-Hao Tsai, Man-Ru Wu, Fan-Li Lin, Jau-Der Ho, Hui-Wen Cheng, Yu-Wen Cheng
TOXICOLOGICAL SCIENCES
(2015)
Article
Toxicology
Cheng-Hui Lin, Man-Ru Wu, Ching-Hao Li, Hui-Wen Cheng, Shih-Hsuan Huang, Chi-Hao Tsai, Fan-Li Lin, Jau-Der Ho, Jaw-Jou Kang, George Hsiao, Yu-Wen Cheng
TOXICOLOGICAL SCIENCES
(2017)
Article
Multidisciplinary Sciences
Fan-Li Lin, Cheng-Hui Lin, Jau-Der Ho, Jing-Lun Yen, Hung-Ming Chang, George C. Y. Chiou, Yu-Wen Cheng, George Hsiao
SCIENTIFIC REPORTS
(2017)
Article
Multidisciplinary Sciences
Po-Lin Liao, Cheng-Hui Lin, Ching-Hao Li, Chi-Hao Tsai, Jau-Der Ho, George C. Y. Chiou, Jaw-Jou Kang, Yu-Wen Cheng
SCIENTIFIC REPORTS
(2017)
Article
Toxicology
Ching-Hao Li, Po-Lin Liao, Ya-Ting Yang, Shih-Hsuan Huang, Cheng-Hui Lin, Yu-Wen Cheng, Jaw-Jou Kang
ARCHIVES OF TOXICOLOGY
(2014)
Article
Multidisciplinary Sciences
Chi-Hao Tsai, Ching-Hao Li, Yu-Wen Cheng, Chen-Chen Lee, Po-Lin Liao, Cheng-Hui Lin, Shih-Hsuan Huang, Jaw-Jou Kang
SCIENTIFIC REPORTS
(2017)
Article
Biochemistry & Molecular Biology
Cheng-Hui Lin, Man-Ru Wu, Wei-Jan Huang, Diana Shu-Lian Chow, George Hsiao, Yu-Wen Cheng
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Developmental Biology
Candace S. Y. Chan, Nicolas Lonfat, Rong Zhao, Alexander E. Davis, Liang Li, Man-Ru Wu, Cheng-Hui Lin, Zhe Ji, Constance L. Cepko, Sui Wang
Article
Immunology
Zuojia Chen, Jialie Luo, Jian Li, Girak Kim, Eric S. Chen, Sheng Xiao, Scott B. Snapper, Bin Bao, Dingding An, Richard S. Blumberg, Cheng-hui Lin, Sui Wang, Jiaxin Zhong, Kuai Liu, Qiyuan Li, Chuan Wu, Vijay K. Kuchroo
Summary: Foxo1 protein regulates mucus secretion by goblet cells to maintain intestinal homeostasis. Loss of Foxo1 leads to defects in autophagy and mucus secretion by goblet cells, impacting gut microbiota and causing increased susceptibility to intestinal inflammation.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Cheng-Hui Lin, Yue Sun, Candace S. Y. Chan, Man-Ru Wu, Lei Gu, Alexander E. Davis, Baokun Gu, Wenlin Zhang, Bogdan Tanasa, Lei R. Zhong, Mark M. Emerson, Lu Chen, Jun B. Ding, Sui Wang
Summary: Adeno-associated viruses (AAVs) can be used as powerful tools to study specific cell types in the central nervous system (CNS). Chromatin accessibility data can be utilized to identify active cis-regulatory modules (CRMs) for AAV-based cell-type specific labeling and manipulation. Strategies such as prescreening based on cell-type-specific transcription factor binding sites (TFBSs) density and generation of synthetic CRMs can improve the efficiency of identifying active CRMs.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Cheng-Hui Lin, Young Joo Sun, Soo Hyeon Lee, Elena M. Mujica, Caitlin R. Kunchur, Man-Ru Wu, Jing Yang, Youn Soo Jung, Bryce Chiang, Sui Wang, Vinit B. Mahajan
Summary: The study presents a cost-effective and minimally invasive protocol, MI3, for delivering ODIs into the mouse vitreous. This approach provides an alternative injection method for small-eyed model organisms and expands the preclinical platforms for evaluating ODIs' efficacy, toxicity, and pharmacokinetics.
Article
Biochemical Research Methods
Young Joo Sun, Cheng-Hui Lin, Man-Ru Wu, Soo Hyeon Lee, Jing Yang, Caitlin R. Kunchur, Elena M. Mujica, Bryce Chiang, Youn Soo Jung, Sui Wang, Vinit B. Mahajan
Summary: The translation discusses the limitations of using small molecule drugs to treat eye diseases and introduces a new method, MI3, to deliver ODIs into mouse eyes. This method aims to expand ODI research to cover a wide range of human eye diseases modeled in mice.
CELL REPORTS METHODS
(2021)
Meeting Abstract
Ophthalmology
Cheng-Hui Lin, Candace Chan, Man-Ru Wu, Alexander Davis, Sui Wang
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Cell Biology
Man-Ru Wu, Cheng-Hui Lin, Jau-Der Ho, George Hsiao, Yu-Wen Cheng
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
(2018)