Journal
TOXICOLOGIC PATHOLOGY
Volume 44, Issue 3, Pages 434-441Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/0192623315609691
Keywords
immunohistochemistry; markers; pig; human; lung; leukocytes; mucus
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Funding
- National Institutes of Health [P01 HL051670, P01 HL091842, P30 DK054759, 1K99HL119560, DP2 HL117744]
- American Asthma Foundation
- Cystic Fibrosis Foundation Research and Development Program
- Cystic Fibrosis Foundation Mucociliary Clearance Consortium
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Genetically engineered pigs are increasingly recognized as valuable models for the study of human disease. Immunohistochemical study of cellular markers of disease is an important tool for the investigation of these novel models so as to evaluate genotype and treatment differences. Even so, there remains a lack of validated markers for pig tissues that can serve as a translational link to human disease in organs such as the lung. Herein, we evaluate markers of cellular inflammation (cluster of differentiation [CD]3, CD79a, B cell lymphoma [BCL] 6, ionized calcium-binding adapter molecule [IBA]1, and myeloperoxidase) and those that may be involved with tissue remodeling (alpha-smooth muscle actin, beta-tubulin-III, lactoferrin, mucin [MUC]5AC, MUC5B, and cystic fibrosis transmembrane conductance regulator [CFTR]) for study of lung tissues. We compare the utility of these markers between pig and human lungs to validate translational relevance of each marker. Our results suggest these markers can be a useful addition in the pathological evaluation of porcine models of human disease.
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