Article
Plant Sciences
Dipesh Dhakal, Sofia Kokkaliari, Garret M. Rubin, Valerie J. Paul, Yousong Ding, Hendrik Luesch
Summary: In this study, a biosynthetic gene cluster (BGC) for Lyngbyastatins (Lbns) was identified and characterized in a marine cyanobacterium. The BGC was found to encode enzymes responsible for the production of compounds 1 and 2, which have anti-cancer activity. Similar BGCs were also identified in the genomes of other marine cyanobacteria.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Biochemistry & Molecular Biology
Julie B. Nielsen, Tetiana Gren, Omkar S. Mohite, Tue S. Jorgensen, Andreas K. Klitgaard, Anna-Sophie Mourched, Kai Blin, Daniel Oves-Costales, Olga Genilloud, Thomas O. Larsen, David Tanner, Tilmann Weber, Charlotte H. Gotfredsen, Pep Charusanti
Summary: Actinomycetes produce a wealth of complex natural products. Researchers have identified the biosynthetic gene cluster for pyracrimycin A and proposed a complete biosynthesis pathway based on feeding experiments and isotope labeling analysis.
ACS CHEMICAL BIOLOGY
(2022)
Article
Plant Sciences
Sergi H. Akone, Joachim J. Hug, Amninder Kaur, Ronald Garcia, Rolf Mueller
Summary: The study reports the discovery, isolation, structure elucidation, and biological evaluation of two new bacterial sterols, nannosterols A and B, from the terrestrial myxobacterium Nannocystis sp. These nannosterols features unique modifications and chemical features, such as a secondary alcohol group at position C-15 and a terminal vicinal diol side chain at C-24-C-25, which have not been observed in bacterial sterols before. The investigation based on myxobacterial high-resolution secondary metabolome data and genomic in silico investigations revealed that nannosterols are frequently produced sterols within the myxobacterial suborder of Nannocystineae. The discovery of nannosterols provides insights into the biosynthesis and evolution of sterols by prokaryotes.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Ecology
Laima Lukoseviciute, Jolanta Lebedeva, Nomeda Kuisiene
Summary: The study aimed to identify and characterize genes responsible for the production of secondary metabolites in the microbial community of one of the deepest caves in the world. Taxonomic diversity analysis showed that most PKS sequences were attributed to Actinobacteria, while NRPS sequences were more diverse. Only three putative metabolites were predicted, indicating the potential for novel secondary metabolites production.
Article
Microbiology
Hisayuki Komaki
Summary: This study aimed to clarify the taxonomic relationships among Streptomyces costaricanus, Streptomyces murinus and Streptomyces phaeogriseichromatogenes using multilocus sequence analysis and bioinformatics methods, revealing that these three strains should be classified as the same species. The presence of a similar set of secondary metabolite-biosynthetic gene clusters for polyketides and nonribosomal peptides further supported their similarity. Therefore, S. costaricanus and S. phaeogriseichromatogenes should be reclassified as later heterotypic synonyms of S. murinus.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Samujjal Bhattacharjee, Ankit Srivastava, Anirbana Parida, Neha Gupta, Prashansha Singh, Satya Shila Singh, Arun Kumar Mishra
Summary: This study demonstrated the bioremediation ability of cyanobacterium Fischerella sp. lmga1 towards Sodium dodecyl sulfate (SDS), as well as the uneven distribution of SDS hydrolysis enzyme in cyanobacteria. The results indicated that lmga1 exhibited strong resilience and removal capability towards SDS.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Madhaiyan Munusamy, Kenneth Tan, Choy Eng Nge, Martin Muthee Gakuubi, Sharon Crasta, Yoganathan Kanagasundaram, Siew Bee Ng
Summary: In this study, 30 Ascomycetes fungi were investigated for their biosynthetic potential and ability to produce antimicrobial secondary metabolites. Screening led to the identification of PKSs and NRPSs genes, suggesting the conserved biosynthesis of closely related compounds from different fungi. Seven compounds were isolated, including Vioxanthin with the best antibacterial activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Peng Hou, Vincent V. Nowak, Chanel J. Taylor, Mark J. Calcott, Allison Knight, Jeremy G. Owen
Summary: In this study, 322 actinomycetes were isolated and their genome sequences were obtained from New Zealand lichens. By enumerating biosynthetic gene clusters (BGCs) and comparing to reference collections, it was found that actinomycetes from New Zealand lichens have the genetic capacity to produce large numbers of natural products, which are expected to be different from those identified in previous soil-based studies. This study sheds light on the actinomycete assemblage in New Zealand lichens and highlights the potential of lichen-sourced actinobacteria for discovering new secondary metabolites.
Review
Chemistry, Physical
Maria L. Adrover-Castellano, Jennifer J. Schmidt, David H. Sherman
Summary: This review explores the general cyclization mechanism of TE domain in NRPS and PKS, including oligomerization and CLCs. It also discusses other cyclization catalysts such as C-T domains, R domains, R-D domains, Dieckmann cyclases, among others. The diversity of cyclization catalysts highlighted in the studies for the production of biologically relevant, complex cyclic natural products and related compounds is summarized here.
Article
Biochemistry & Molecular Biology
Lauren A. Mills, Jose Angel Moreno-Cabezuelo, Artur Wlodarczyk, Angelo J. Victoria, Rebeca Mejias, Anja Nenninger, Simon Moxon, Paolo Bombelli, Tiago T. Selao, Alistair J. McCormick, David J. Lea-Smith
Summary: PCC 11901 is a cyanobacterium that demonstrates the highest and most sustained growth under optimized conditions. It has superior light use, reduced photoinhibition, and higher photosynthetic and respiratory rates compared to other model cyanobacteria. This study establishes PCC 11901 as a promising species for cyanobacterial biotechnology and provides valuable tools and strains for advancing this field.
Article
Multidisciplinary Sciences
Bo Pang, Rijing Liao, Zhijun Tang, Shengjie Guo, Zhuhua Wu, Wen Liu
Summary: The study elucidates the biosynthetic pathways of caerulomycins and collismycins, validates the generality of 2,2'-bipyridine formation, and clarifies the process for 2,2'-bipyridine furcation.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Mahir Bozan, Denny Popp, Rene Kallies, Ulisses Nunes da Rocha, Stephan Klaehn, Katja Buehler
Summary: Cyanobacteria have great potential in biotechnologies, and this study focuses on the development of molecular tools for genetic engineering, as well as the design of chassis strains and novel reactor concepts. The genome sequence of Tolypothrix sp. PCC 7712 is provided, and it is found to have high similarities with Tolypothrix sp. PCC 7601, but also some specific variations. These findings are important for targeted strain engineering.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Engineering, Environmental
Javier Garcia-Calleja, Thibaut Cossart, Zoyne Pedrero, Joao P. Santos, Laurent Ouerdane, Emmanuel Tessier, Vera Slaveykova, David Amouroux
Summary: Understanding the complexation of mercury (Hg) with low molecular weight (LMW) bioligands can help elucidate its speciation, and the use of isotopically labeled Hg species in cyanobacteria has provided insights into the role of intracellular biogenic ligands in the speciation of iHg and MeHg.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Biochemical Research Methods
Maurice Mager, Hugo Pineda Hernandez, Fabian Brandenburg, Luis Lopez-Maury, Alistair J. McCormick, Dennis J. Nuernberg, Tim Orthwein, David A. Russo, Angelo Joshua Victoria, Xiaoran Wang, Julie A. Z. Zedler, Filipe Branco dos Santos, Nicolas M. Schmelling
Summary: In recent years, many new synthetic biology tools have been developed for use in cyanobacteria. However, the reported characterizations of these tools often cannot be reproduced, which greatly limits comparability of results and applicability. This study assessed the reproducibility of a standard microbiological experiment in Synechocystis sp. PCC 6803 and found significant differences in spectrophotometer measurements and growth rates between laboratories. These findings highlight the need for additional reporting requirements of growth conditions for phototrophic organisms beyond light intensity and CO2 supply.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Wei-Yue Xing, Jing Liu, Ju-Yuan Zhang, Xiaoli Zeng, Cheng-Cai Zhang
Summary: In this study, a new negative regulator called PatU3 was identified in the prokaryote Anabaena, which regulates cell division and heterocyst development. The results showed that PatU3 is a specific substrate of the protease activity of HetF, and HetF controls cell division and heterocyst differentiation by controlling the inhibitory effects of PatU3.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Claire Pancrace, Keishi Ishida, Enora Briand, Douglas Gatte Pichi, Annika R. Weiz, Arthur Guljarnow, Thibault Scalvenzi, Nathalie Sassoon, Christian Hertweck, Elke Dittmann, Muriel Gugger
ACS CHEMICAL BIOLOGY
(2019)
Article
Biology
Alexis De Wever, Karim Benzerara, Margot Coutaud, Geraldine Caumes, Melanie Poinsot, Feriel Skouri-Panet, Thierry Laurent, Elodie Duprat, Muriel Gugger
Article
Biochemistry & Molecular Biology
Eric J. N. Helfrich, Reiko Ueoka, Alon Dolev, Michael Rust, Roy A. Meodedl, Agneya Bhushan, Gianmaria Califano, Rodrigo Costa, Muriel Gugger, Christoph Steinbeck, Pablo Moreno, Jorn Piel
NATURE CHEMICAL BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Aysegul Dilsizoglu Senol, Anna Repe, Clara Grudina, Nathalie Sassoon, Ueoka Reiko, Luc Bousset, Ronald Melki, Joern Pie, Muriel Guggert, Chiara Zurzolo
SCIENTIFIC REPORTS
(2019)
Article
Multidisciplinary Sciences
Sandra Kim Tiam, Katia Comte, Caroline Dalle, Charlotte Duval, Claire Pancrace, Muriel Gugger, Benjamin Marie, Claude Yepremian, Cecile Bernard
Article
Food Science & Technology
Sandra Kim Tiam, Muriel Gugger, Justine Demay, Severine Le Manach, Charlotte Duval, Cecile Bernard, Benjamin Marie
Article
Biochemistry & Molecular Biology
Antti Mattila, Rose-Marie Andsten, Mikael Jumppanen, Michele Assante, Jouni Jokela, Matti Wahlsten, Kornelia M. Mikula, Cihad Sigindere, Daniel H. Kwak, Muriel Gugger, Harri Koskela, Kaarina Sivonen, Xinyu Liu, Jari Yli-Kauhaluoma, Hideo Iwai, David P. Fewer
ACS CHEMICAL BIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Thorsten Bauersachs, Scott R. Miller, Muriel Gugger, Opayi Mudimu, Thomas Friedl, Lorenz Schwark
Article
Biochemistry & Molecular Biology
Nadia Melo, Gabriella H. Wolff, Andre Luis Costa-da-Silva, Robert Arribas, Merybeth Fernandez Triana, Muriel Gugger, Jeffrey A. Riffell, Matthew DeGennaro, Marcus C. Stensmyr
Article
Chemistry, Multidisciplinary
Nina M. Bosch, Mariana Borsa, Ute Greczmiel, Brandon I. Morinaka, Muriel Gugger, Annette Oxenius, Anna L. Vagstad, Jorn Piel
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2020)
Article
Chemistry, Multidisciplinary
Philipp Moosmann, Felix Ecker, Stefan Leopold-Messer, Jackson K. B. Cahn, Cora L. Dieterich, Michael Groll, Joern Piel
Article
Microbiology
Angela Brito, Jorge Vieira, Cristina P. Vieira, Tao Zhu, Pedro N. Leao, Vitor Ramos, Xuefeng Lu, Vitor M. Vasconcelos, Muriel Gugger, Paula Tamagnini
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Chemistry, Multidisciplinary
Thi Quynh Ngoc Nguyen, Yi Wei Tooh, Ryosuke Sugiyama, Thi Phuong Diep Nguyen, Mugilarasi Purushothaman, Li Chuan Leow, Karyna Hanif, Rubin How Sheng Yong, Irene Agatha, Fernaldo R. Winnerdy, Muriel Gugger, Anh Tuan Phan, Brandon Morinaka
Article
Plant Sciences
Meng Li, Alexandra Calteau, Dmitry A. Semchonok, Thomas A. Witt, Jonathan T. Nguyen, Nathalie Sassoon, Egbert J. Boekema, Julian Whitelegge, Muriel Gugger, Barry D. Bruce
