Article
Spectroscopy
Chunpo Ge, Huayu Wang, Tianjun Ni, Zhijun Yang, Kaiwen Chang
Summary: A red-emitting probe TMN-NCS based on ITC structure was proposed in this paper, which has high selectivity and sensitivity to Cys and shows great potential for biological applications with low toxicity, low detection limit, and good cell permeability.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Spectroscopy
Boxin Zhou, Beibei Wang, Mengqi Bai, Mingdong Dong, Xu Tang
Summary: Cysteine plays an important role in the human body, and abnormal concentration can lead to various diseases. Detecting cysteine in vivo with high selectivity and sensitivity is therefore significant. In this study, a small organic fluorescent probe ZHJ-X was designed and synthesized based on cyanobiphenyl, which specifically recognized cysteine. The probe ZHJ-X demonstrated specific selectivity, high sensitivity, short response time, good anti-interference ability, and a low detection limit of 3.8 x 10-6 M. It was successfully applied for visualizing cysteine in living cells and holds potential for cell imaging and detection.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Yuewei Zhang, Ning Zhang, Shuohang Wang, Qi Zan, Xiaodong Wang, Qianqian Yang, Xue Yu, Chuan Dong, Li Fan
Summary: In this study, a LD-targetable and biothiol-sensitive fluorescent probe, BTDA-RSS, was developed to distinguish cancerous cells/tissues from normal cells/tissues. The probe displayed a rapid and marked fluorescence turn-on response toward biothiols, and has the potential to visualize LD biothiols in surgical specimens from cancer patients.
Article
Spectroscopy
Liuqi Qiao, Yongxing Yang, Jianhua Cai, Xin Lv, Junsheng Hao, Yaping Li
Summary: The fluorescent probe PA-A shows high selectivity, sensitivity, and low detection limit for detecting Cys, with the added benefit of being visible to the naked eye. Fluorescence imaging experiments in cells successfully demonstrated excellent tissue penetration.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Analytical
Xianzhi Chai, Bohan Li, Chi Chen, Weijian Zhang, Lulu Sun, Hai-Hao Han, Yafei Zhang, Shasha Sun, Jianming Yang, Junji Zhang, Xiao-Peng He
Summary: Drug-induced liver injury (DILI) is a clinical issue associated with many commercial drugs, and traditional methods cannot monitor the dynamic changes in peroxynitrite (ONOO-) levels during DILI in real time. Therefore, the development of ONOO--activated near-infrared (NIR) fluorescent probes with high sensitivity and selectivity is crucial for early diagnosis of DILI.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Shaolong Qi, Haiyan Zhang, Xinyu Wang, Jialin Lv, Dahai Liu, Wenbin Shen, Yaoxian Li, Jianshi Du, Qingbiao Yang
Summary: Cysteine plays vital roles in the human body, and detecting it with high selectivity and sensitivity in vivo is crucial. Near-infrared fluorescent probes are widely used in biological detection, but there are few probes that can detect Cys. By synthesizing a specific structure NIR probe, high selectivity detection of Cys can be achieved, showing excellent performance and promising applications.
Article
Chemistry, Analytical
Wei Wang, Min Ji, Junqing Chen, Peng Wang
Summary: In this study, a turn-on type aggregation-induced emission (AIE) fluorescent probe BQM-NBD was developed for selective detection of Cys and Hcy in organisms, showing good selectivity. Additionally, BQM-NBD exhibited low cytotoxicity when used for imaging Cys in living cells.
Article
Chemistry, Multidisciplinary
Ya-Xi Ye, Ya-Wen Yu, Zhu-Min Xu, Xin-Yue Chen, Zhong-Chang Wang, Bao-Zhong Wang, Qing-Cai Jiao, Hai-Liang Zhu
Summary: A novel fluorescent probe, PE-YW, was designed for selectively detecting Cys by regulating its AIE-based effects. The probe utilized a specific conjugate addition cyclization reaction between Cys and an acrylate moiety to induce fluorescence turn-on. PE-YW showed high selectivity, low detection limit, and excellent performance for detecting Cys in living systems.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Fung-Kit Tang, Yanyan Chen, Daniel Nnaemaka Tritton, Zongwei Cai, Ken Cham-Fai Leung
Summary: A class of rhodamine-based fluorescent sensors that can selectively and sensitively detect Pd2+ metal ions in aqueous media have been developed. These sensors, PMS and PRS, exhibit colorimetric and fluorescent changes when exposed to Pd2+, due to the opening and restoring of their spirolactam rings. PRS has high selectivity for Pd2+ over 22 other metal ions, with a 0.6-fold ratiometric difference at I-600nm/I-515nm. The closed form of the lactam ring in PRS-Pd can be switched back in the presence of various thiols, providing a red-green traffic light detection mechanism. PRS also shows excellent cell viability and can be used to image Pd2+ and detect biothiols in cancer cells.
CHEMISTRY-AN ASIAN JOURNAL
(2023)
Article
Chemistry, Analytical
Chen Xu, Yukun Zhang, Hui Sun, Jindong Ai, Mingguang Ren
Summary: We have developed a new two-photon fluorescent probe (T-HS) for the detection of H2S. The probe exhibits regular changes in absorption and fluorescence spectra upon addition of hydrogen sulfide. It demonstrates advantageous properties such as a large turn-on fluorescence signal, good selectivity, and low cytotoxicity. Furthermore, the probe T-HS has been successfully utilized for fluorescence imaging of H2S in live cells and zebrafish.
ANALYTICAL METHODS
(2023)
Article
Biochemistry & Molecular Biology
Cheng Zheng, Xiaoqin Zhou, Huizhe Wang, Min Ji, Peng Wang
Summary: In this study, a novel ratiometric fluorescent probe N-1 was designed for sensitive and selective detection of cysteine. It showed excellent sensitivity and selectivity to cysteine and could be used for imaging cysteine in living cells. This probe has potential biomedical application value.
BIOORGANIC CHEMISTRY
(2022)
Article
Spectroscopy
Yongpeng Wang, Jia Chen, Yang Shu, Jianhua Wang, Hongdeng Qiu
Summary: In this study, a fluorescent probe TNP-SBD-Cl was developed to specifically identify cysteine (Cys) and avoid interference from other biological analytes. The probe can monitor Cys in living HeLa cells with low cytotoxicity, showing significant diagnostic potential in physiological and pathological processes.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Biochemistry & Molecular Biology
Wei Wang, Zihao Peng, Min Ji, Junqing Chen, Peng Wang
Summary: In this study, a fluorescent probe SQM-NBD was synthesized for selective detection of Cys/Hcy. The probe showed superior sensitivity to Cys/Hcy and was able to achieve fluorescence imaging of Cys/Hcy in HeLa cells, demonstrating its potential for Cys/Hcy identification under physiological and pathological conditions.
BIOORGANIC CHEMISTRY
(2022)
Article
Spectroscopy
Rui Guo, Qing Liu, Yonghe Tang, Shushun Cai, Xiaoya Li, Xi Gong, Yingjie Yang, Weiying Lin
Summary: Small molecule biological thiols play crucial roles in maintaining cellular activities and abnormal levels are associated with diseases. A new fluorescent probe Ty-Cys1 was designed in this study for effective monitoring of Cys changes.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Optics
Zongyue Cheng, Yiyong Han, Bowen Wei, Baoling Lai, Baoming Li, Meng Cui, Wen-Biao Gan
Summary: The AFL-TPM system developed in this study enables targeted cell ablation and real-time monitoring of neuronal network changes in the mouse cortex. Precise control of the laser power for ablation revealed that the ablation of certain cells can increase the activity of nearby neurons, and also demonstrated the structural and functional independence of different dendritic branches.
Article
Chemistry, Multidisciplinary
Yonghwang Ha, Dhiraj P. Murale, Changsuk Yun, Sudesh T. Manjare, Hyungjun Kim, Juhyoun Kwak, Yoon Sup Leea, David G. Churchill
CHEMICAL COMMUNICATIONS
(2015)
Article
Chemistry, Multidisciplinary
Dhiraj P. Murale, Seong Cheol Hong, JiHyeon Yun, Chang No Yoon, Jun-Seok Lee
CHEMICAL COMMUNICATIONS
(2015)
Article
Chemistry, Multidisciplinary
Yonghwang Ha, Dhiraj P. Murale, Sudesh T. Manjare, Minseong Kim, Jeong A. Jeong, David G. Churchill
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
(2016)
Article
Biochemical Research Methods
Seong Cheol Hong, Dhiraj P. Murale, Jun-Seok Lee
COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING
(2016)
Review
Biochemical Research Methods
Dhiraj P. Murale, Seong Cheol Hong, Md. Mamunul Haque, Jun-Seok Lee
Article
Biochemistry & Molecular Biology
Dhiraj P. Murale, Seong Cheol Hong, Md Mamunul Haque, Jun-Seok Lee
Article
Chemistry, Multidisciplinary
Dhiraj P. Murale, Hwajin Kim, Wan Sung Choi, David G. Churchill
Article
Biochemistry & Molecular Biology
Dhiraj P. Murale, Seong Cheol Hong, Se-young Jang, Jun-Seok Lee
Review
Biochemistry & Molecular Biology
Se-Young Jang, Dhiraj P. Murale, Anne Doyoung Kim, Jun-Seok Lee
Review
Biochemistry & Molecular Biology
Md. Mamunul Haque, Dhiraj P. Murale, Yun Kyung Kim, Jun-Seok Lee
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Review
Biochemistry & Molecular Biology
Md Mamunul Haque, Dhiraj P. Murale, Jun-Seok Lee
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Chemistry, Multidisciplinary
Dhiraj P. Murale, Md Mamunul Haque, Kyung Tae Hong, Jun-Seok Lee
Summary: The schematic illustrates the use of pyridinyl-pyrazole BODIPY for lipid droplet imaging.
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
(2021)
Review
Biochemistry & Molecular Biology
Nicholas Asiimwe, Mohammad Faysal Al Mazid, Dhiraj P. Murale, Yun Kyung Kim, Jun-Seok Lee
Summary: N-terminal protein modifications play critical roles in cellular processes, and chemical probes are important tools for studying these modifications. N-terminal cysteine is a practical option for overcoming regioselectivity challenges.
Article
Chemistry, Applied
Dhiraj P. Murale, Md Mamunul Haque, Seong Cheol Hong, Se-young Jang, Jung Hoon Lee, Seo Jeong An, Jun-Seok Lee
Summary: By synthesizing five BODIPY fluorophores and investigating their photophysical and photo-crosslinking properties, pcBDaza-Mito was discovered to selectively stain mitochondria in live-cell conditions and exhibit a 2.3-fold increase in photo-crosslinking efficiency to mitochondria proteins. These results demonstrate that the bifunctional BODIPY probe has great potential for robust mitochondria imaging in live cells.
Article
Chemistry, Multidisciplinary
Seong Cheol Hong, Dhiraj P. Murale, Minju Lee, Seung Mi Lee, Joong Shin Park, Jun-Seok Lee
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2017)