Journal
THROMBOSIS RESEARCH
Volume 135, Issue 1, Pages 121-126Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.thromres.2014.10.010
Keywords
Platelet; Thalassemia; Tricuspid regurgitant velocity; Pulmonary arterial pressure; Plasma hemoglobin; Hemolysis
Categories
Funding
- DPST [024/2557]
- Office of the Higher Education Commission and Mahidol University under the National Research Universities Initiative, and Mahidol University
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Introduction: Pulmonary arterial hypertension is a life-threatening complication in thalassemia characterized by elevated pulmonary arterial pressure. Increased platelet activation is associated with this complication; however, its role remains unclear. Methods: Platelet activation in splenectomized beta-thalassemia/hemoglobin E (Hb E) patients was measured using flow cytometric determination of P-selectin and activated glycoprotein (aGP) IIb/IIIa expression, and plateletleukocyte aggregates (platelet-neutrophil, platelet-monocyte and platelet-lymphocyte aggregates). Tricuspid regurgitant velocity (TRV) was measured and used as an indicator of pulmonary arterial pressure. Plasma hemoglobin served as markers of hemolysis. Results: Fifteen of 27 patients had elevated TRV (>2.5 m/s). Platelet expression of P-selectin and aGPIIb/IIIa, and platelet-leukocyte aggregates were higher in thalassemia patients with elevated TRV than healthy control. Platelet-neutrophil aggregates increased in thalassemia patients with elevated TRV compared to patients with normal TRV. The increase in P-selectin and aGPIIb/IIIa expression induced by adenosine diphosphate (ADP) was higher in patients with elevated TRV than those with normal TRV. Platelet P-selectin expression and platelet-neutrophil aggregates correlated positively with TRV. Plasma hemoglobin levels in patients with elevated TRV were higher than those of the control subjects, and correlated with TRV. Conclusion: Thalassemia patients with elevated TRV have a further increase in platelet activation that correlates with hemolysis. (C) 2014 Elsevier Ltd. All rights reserved.
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