Article
Immunology
Ehsan Gharib, Vanessa Veilleux, Luc H. Boudreau, Nicolas Pichaud, Gilles A. Robichaud
Summary: Inflammation and platelets promote cancer malignancy. Platelet-derived microparticles (PMPs) induce metabolic plasticity and disease progression in chronic lymphocytic leukemia (CLL). PMPs enhance CLL tumor growth and invasiveness through mitochondrial internalization and OXPHOS stimulation, as well as increase resistance to chemotherapy drugs.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Marta Brambilla, Maria Talmon, Paola Canzano, Luigia G. Fresu, Sandra Brunelleschi, Elena Tremoli, Marina Camera
Summary: This study evaluated the effects of microvesicles derived from platelets and monocytes on endothelial function, and found that platelet-derived microvesicles can impair endothelial function in the acute phase.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Hematology
E. O. Artemenko, S. I. Obydennyi, K. S. Troyanova, G. A. Novichkova, D. Y. Nechipurenko, M. A. Panteleev
Summary: In this study, we investigated the adhesive properties of circulating plasma-derived and platelet-derived microvesicles (MVs) and their potential role in thrombus growth. The results showed that both types of MVs weakly bound fibrinogen but did not stably adhere to immobilized fibrinogen. Moreover, the presence of these MVs did not significantly affect thrombus formation. Therefore, MVs present in the plasma of healthy individuals are unlikely to directly contribute to thrombus formation under arterial flow conditions.
THROMBOSIS RESEARCH
(2024)
Article
Multidisciplinary Sciences
Zhaohan Wei, Xiaoqiong Zhang, Tuying Yong, Nana Bie, Guiting Zhan, Xin Li, Qingle Liang, Jianye Li, Jingjing Yu, Gang Huang, Yuchen Yan, Zelong Zhang, Bixiang Zhang, Lu Gan, Bo Huang, Xiangliang Yang
Summary: This study demonstrates that metformin-loaded mannose-modified macrophage-derived microparticles can reprogram the tumor immune microenvironment, enhance responses to anti-PD-1 therapy, and promote infiltration of CD8(+) T cells into tumor tissues.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Hyun Ji Kim, Matthew J. Rames, Samuel Tassi Yunga, Randall Armstrong, Mayu Morita, Anh T. P. Ngo, Owen J. T. McCarty, Fehmi Civitci, Terry K. Morgan, Thuy T. M. Ngo
Summary: This study examined the impact of differential centrifugation and freeze/thaw on the profiles of extracellular vesicles (EVs) and cell-free messenger RNA (cf-mRNA) in plasma using flow cytometric analysis. The results revealed that the differential centrifugation and post-freeze/thaw processing affected the EV subpopulations and cf-mRNA levels. Freezing plasma containing residual platelets resulted in irreversible ex vivo generation of EV subpopulations and cf-mRNA transcripts.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Asmaa M. Zahran, Omnia El-Badawy, Wageeh A. Ali, Zainab Gaber Mahran, Essam Eldeen M. O. Mahran, Amal Rayan
Summary: This study found significant differences in hematologic parameters between COVID-19 malignant and non-malignant patients, with a significant accumulation of microparticles and activated platelets in the blood of affected patients. These results suggest that circulating microparticles and activated platelets may be promising prognostic biomarkers for the outcome of COVID-19 patients.
Review
Pharmacology & Pharmacy
Zhenghua Li, Zhen Liu, Jiacai Wu, Bin Li
Summary: This review provides an overview of recent advances in naturally occurring mRNA delivery platforms and their biomedical applications, as well as discusses the future perspectives on the clinical translation of cell-derived vesicles.
Article
Immunology
Minjie Xu, Rong Du, Wenping Xing, Xueting Chen, Jian Wan, Shengqing Wang, Li Xiong, Kutty Selva Nandakumar, Rikard Holmdahl, Hui Geng
Summary: Citrullinated neoepitopes have been identified as key triggers for the synthesis of anti-citrullinated protein antibodies (ACPA) in rheumatoid arthritis (RA) patients. This study investigated the expression of citrullinated proteins and enzymes in human platelets and platelet-derived microparticles (PDP). The results revealed the presence of citrullinated autoantigens in platelets and PDPs, suggesting that they could be potential targets for ACPA in RA. These findings provide valuable insights into the role of platelets in triggering ACPA production in RA.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Analytical
Gugu Kubheka, Estela Climent, Charlie Tobias, Knut Rurack, John Mack, Tebello Nyokong
Summary: This study used azaBODIPY dyes doped into polystyrene particles as optical probes for the detection of high-risk HPV types. The particles were successfully attached with HPV DNA single strands, and demonstrated low detection limits and a narrow detection range for HPV11. The results suggest that these particles have the potential for multiplex assays and further study.
Article
Biochemistry & Molecular Biology
Matteo Sibilano, Valentina Tullio, Gaspare Adorno, Isabella Savini, Valeria Gasperi, Maria Valeria Catani
Summary: Platelets release microvesicles that modulate cell growth and dissemination in breast cancer. Enriched miR-126 in these microvesicles can suppress cell proliferation and invasion by targeting AKT2. High miR-126 and low AKT2 levels are associated with favorable prognosis in breast cancer patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Rosa Suades, Teresa Padro, Gemma Vilahur, Lina Badimon
Summary: Platelets play essential roles in thrombosis, inflammation, and angiogenesis. Stimulation by thrombin triggers the release of pEVs from platelets, which are rich in procoagulant and proadhesive proteins. Differential proteomic analysis revealed significant changes in protein composition between pEVs from activated platelets and non-activated platelets.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cell Biology
Pooja Yadav, Abhishek R. Panigrahi, Samir K. Beura, Sunil K. Singh
Summary: Platelet-derived microvesicles (PMVs) are an important component of microvesicles in circulation and have been associated with various pathophysiological complications. Recent research shows that PMVs carry significant cargo that can influence cellular functions by affecting calcium oscillations in target cells. This study aimed to investigate the impact of PMVs on platelet calcium mobilization. It found that PMVs increase platelet intracellular calcium levels in a dose-dependent manner by utilizing intracellular storage and extracellular space.
CELL BIOLOGY INTERNATIONAL
(2023)
Article
Nanoscience & Nanotechnology
Wenjie Zhao, Jiawei Hu, Jinlong Liu, Xin Li, Sheng Sun, Xiaofeng Luan, Yang Zhao, Shuhua Wei, Mingxiao Li, Qingzhu Zhang, Chengjun Huang
Summary: In this study, a Si nanowire field-effect transistor (Si-NW Bio-FET) device modified with specific antibodies was proposed for the electrical and label-free detection of exosomes. The developed Si-NW Bio-FET showed high sensitivity and rapid response, allowing for direct quantification and real-time detection of exosomes without labeling, indicating its potential as a tool for early cancer diagnosis.
MICROSYSTEMS & NANOENGINEERING
(2022)
Review
Biochemistry & Molecular Biology
Hara T. Georgatzakou, Sotirios P. Fortis, Effie G. Papageorgiou, Marianna H. Antonelou, Anastasios G. Kriebardis
Summary: Microvesicles, formed by outward budding of the plasma membrane, play a vital role in intercellular communication, material disposal, and membrane repair. Changes in their concentration or components are associated with various diseases.
Article
Materials Science, Multidisciplinary
Congcong Rong, Jianan Zhang, Song Chen, Yixuan Wang, Toshiyuki Ikoma, Xiaona Li, Weiyi Chen
Summary: Silica hollow microspheres were prepared as biocompatible cell carriers using emulsified gelatin microspheres as sacrificial templates. SEM observations showed their spherical morphology with a hollow structure and a diameter of 50-200 μm. FT-IR spectrum revealed the presence of Si-O-Si bonds. Gelatin microspheres served as supporting matrix and sacrificial templates for the formation of hollow structure. The silica hollow microspheres were found to be biocompatible and supported cell attachment and proliferation when seeded with osteoblast MC3T3-E1 cells, indicating their potential as biocompatible cell carriers.
Article
Hematology
E. O. Artemenko, S. I. Obydennyi, K. S. Troyanova, G. A. Novichkova, D. Y. Nechipurenko, M. A. Panteleev
Summary: In this study, we investigated the adhesive properties of circulating plasma-derived and platelet-derived microvesicles (MVs) and their potential role in thrombus growth. The results showed that both types of MVs weakly bound fibrinogen but did not stably adhere to immobilized fibrinogen. Moreover, the presence of these MVs did not significantly affect thrombus formation. Therefore, MVs present in the plasma of healthy individuals are unlikely to directly contribute to thrombus formation under arterial flow conditions.
THROMBOSIS RESEARCH
(2024)
Letter
Hematology
Giuseppe Lippi, Camilla Mattiuzzi, Emmanuel J. Favaloro
THROMBOSIS RESEARCH
(2024)
Article
Hematology
Marium Malik, Maha Al-Ghafry, Abraham Haimed, Julia Su, Maribel Lema, Linda Shore-Lessersson, Suchitra S. Acharya
Summary: This study analyzed and evaluated leukemia-associated coagulopathy in newly diagnosed pediatric leukemia patients using CCTs and ROTEM. The results showed that fibrinogen and platelets played a significant role in causing coagulopathies in this population. This suggests the utility of using CCTs and ROTEM in the pediatric population to evaluate hemostatic function and guide blood product replacement.
THROMBOSIS RESEARCH
(2024)
Review
Hematology
Mohammad M. Elahi, Alexandra N. Witt, Edward L. G. Pryzdial, Paul B. McBeth
Summary: Thrombotic disease may be underdiagnosed in microgravity conditions, and the underlying factors are still poorly understood. A case of internal jugular vein thrombosis was recently diagnosed in a low-risk female astronaut, and six out of ten additional crew members showed risk factors for jugular venous flow. Observations in space and studies on Earth suggest that microgravity affects blood flow, coagulation, and vascular function.
THROMBOSIS RESEARCH
(2024)
Review
Hematology
Stian Ingemann-Molden, Christina Krogner Caspersen, Nanna Rolving, Anette Arbjerg Hojen, Frederikus A. Klok, Erik L. Grove, Barbara Cristina Brocki, Jane Andreasen
Summary: This study found that no single PROM covered all aspects considered important by PE patients. PROMs recommended in the ICHOM core set covered most aspects, but worrisome thoughts, hypervigilance around symptoms, and uncertainty of illness were not covered.
THROMBOSIS RESEARCH
(2024)
Article
Hematology
Haomin Yan, Tsutomu Sasaki, Yasufumi Gon, Kumiko Nishiyama, Hideaki Kanki, Hideki Mochizuki
Summary: The incidence of cancer-associated stroke has increased with the prolonged survival times of cancer patients. In this study, the researchers focused on the driver gene KRAS and found that KRAS mutations exacerbated neurological deficits after stroke and promoted tumor growth. They also identified the upregulation of pro-inflammatory responses and the promotion of STAT3 phosphorylation in mice with KRAS mutations. Additionally, the researchers found that colorectal cancer patients with stroke onset within 3 months after cancer diagnosis had a worse prognosis.
THROMBOSIS RESEARCH
(2024)
Letter
Hematology
Tomoki Togashi, Satomi Nagaya, Makiko Meguro-Horike, Haruto Matsumoto, Yuta Imai, Koichi Yamaguchi, Yoshinari Fujii, Haruka Moriya, Yuika Kikuchi, Ibuki Yasuda, Shin-ichi Horike, Eriko Morishita
THROMBOSIS RESEARCH
(2024)
Article
Hematology
Marta Najarro, Winnifer Briceno, Carmen Rodriguez, Alfonso Muriel, Sara Gonzalez, Ana Castillo, Ignacio Jara, Parth Rali, Catalin Toma, Behnood Bikdeli, David Jimenez
Summary: The CPES score is a useful tool in identifying the risk of hemodynamic deterioration in normotensive patients with acute PE, with acceptable C-statistic, excellent specificity, and low positive predictive value.
THROMBOSIS RESEARCH
(2024)
Article
Hematology
Lei Liu, Huifang Xu, Jian Wang, Haiyan Wang, Saisai Ren, Qian Huang, Mingyan Zhang, Hui Zhou, Chunyan Yang, Lu Jia, Yu Huang, Hao Zhang, Yanling Tao, Ying Li, Yanan Min
Summary: This study investigated the gut microbiota metabolites and cytokines, chemokines, and growth factors in patients with ITP and found their association with treatment responsiveness. The findings suggest that imbalanced secretion of cyto(chemo)kins/growth factors and inadequate levels of TMAO are characteristic of patients with ITP.
THROMBOSIS RESEARCH
(2024)
Article
Hematology
Roxanne Mcknight, Mohammad Salameh, Lee Ann Jones, Greta Anton, Lindsay Carter
Summary: This study evaluated the safety of fixed-dose heparin titration boluses in underweight patients with VTE. The results showed that although underweight patients required more frequent holding of heparin, there was no significant increase in bleeding risk.
THROMBOSIS RESEARCH
(2024)