Article
Biochemistry & Molecular Biology
Magdalena Klimek-Chodacka, Miron Gieniec, Rafal Baranski
Summary: The study demonstrates an efficient, recombinant DNA-free, multiplex gene-editing method using gRNA:Cas9 ribonucleoprotein (RNP) complexes delivered directly to plant protoplasts. By forming three RNPs in vitro and delivering them to protoplasts, various editing effects were achieved with high efficiency, showcasing the potential of this method.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Tomasz Oleszkiewicz, Michal Kruczek, Rafal Baranski
Summary: This study demonstrates that the accumulation of carotenoids in carrot cells is affected by nitrogen concentration and the ratio of nitrate to ammonium, while other components in the media do not significantly impact carotenoid content or callus growth.
Article
Chemistry, Applied
Bruno Stefani Esposto, Sabrina Guarda Botelho Pinho, Marcelo Thomazini, Ana Paula Ramos, Delia Rita Tapia-Blacido, Milena Martelli-Tosi
Summary: This study aimed to extract bioactive compounds from carrot by-products and evaluated their chemical stability after encapsulation in chitosan-coated TPP-Ch particles. The results showed that the encapsulation efficiency for carotenoids and total phenolic compounds reached over 75%. Furthermore, the TPP-Ch particles demonstrated better stability for carotenoids in various experimental conditions, making them suitable for application in the food industry and aqueous matrices.
Article
Biotechnology & Applied Microbiology
Abraham Palacios-Velasquez, Violeta Quispe-Coquil, Enzo Martin Casimiro-Soriano, Karla Milagros Tapia-Zarate, Alex Ruben Huaman-de la Cruz
Summary: Bioethanol was successfully obtained from carrot residues using a specific enzymatic hydrolysis and fermentation process. The resulting product exhibited superior ethanol content and net calorific value compared to previous studies.
FERMENTATION-BASEL
(2023)
Article
Plant Sciences
Maria Belen Perez, Sofia Carvajal, Vanesa Beretta, Florencia Bannoud, Maria Florencia Fangio, Federico Berli, Ariel Fontana, Maria Victoria Salomon, Roxana Gonzalez, Lucia Valerga, Jorgelina C. Altamirano, Mehtap Yildiz, Massimo Iorizzo, Philipp W. Simon, Pablo F. Cavagnaro
Summary: The present study characterized a diverse collection of purple and non-purple carrot accessions for their concentration of anthocyanins, phenolics, carotenoids, and antioxidant capacity. Significant variation was found among the accessions, with acylated anthocyanins being the most dominant pigment. Purple carrots exhibited higher levels of anthocyanins, phenolics, and antioxidant capacity compared to non-purple carrots. Antioxidant capacity was positively correlated with anthocyanin content and total phenolic content, but not with carotenoids. Certain accessions were identified as potential sources for food dyes or increased nutraceutical value.
Article
Plant Sciences
Petar Mitrovic, Ivica Djalovic, Biljana Kiprovski, Sonja Veljovic Jovanovic, Vojislav Trkulja, Aleksandra Jelusic, Tatjana Popovic
Summary: The study found that infection by Candidatus Phytoplasma solani caused abnormal growth in carrot plants, leading to symptoms such as leaf chlorosis and reddening, affecting antioxidative metabolism and chlorophyll content.
Article
Biotechnology & Applied Microbiology
Muhammad Omer Iqbal, Asad Saleem Sial, Imran Akhtar, Muhammad Naeem, Abu Hazafa, Rais A. Ansari, Syed A. A. Rizvi
Summary: The study investigated the nephroprotective effects of Daucus carota and Eclipta prostrata extracts on cisplatin-induced nephrotoxicity, showing significant improvements at a dose rate of 600 mg/kg.
Article
Environmental Sciences
Samia Faiz, Anis Ali Shah, Naima Huma Naveed, Anila Nijabat, Nasim Ahmad Yasin, Aima Iram Batool, Hayssam M. Ali, Talha Javed, Phillip W. Simon, Aamir Ali
Summary: The study investigated the synergistic role of AgNPs and IAA in improving resilience against Cd toxicity in carrot plants, enhancing plant growth attributes and detoxifying reactive oxygen species. Genotypic variation for Cd tolerance in carrots was observed, with Pre breed 22 identified as a tolerant genotype with increased Cd content in roots compared to shoots.
Article
Green & Sustainable Science & Technology
Anna Bonasia, Giulia Conversa, Corrado Lazzizera, Giuseppe Gambacorta, Antonio Elia
Summary: The exploration in the sandy shores of Salterns of Margherita di Savoia revealed the cultivation of local carrot landraces with high nutritional and nutraceutical properties, driven by the pedological conditions of the area. Variability in morpho-qualitative traits among carrot genotypes and the potential of a specific landrace, Carota a punta tonda, as a healthy product rich in phenols and beta-carotene were identified, suggesting the importance of on-farm conservation for biodiversity preservation.
Review
Food Science & Technology
Gemala Anjani, Fitriyono Ayustaningwarno, Rafika Eviana
Summary: Carrots are rich in bioactive compounds that have immunomodulatory effects, contributing to their health benefits. They regulate immune response through anti-inflammatory, antioxidant, and overall immune response modulation mechanisms.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Plant Sciences
Vincenzo D'Amelia, Julien Curaba, Muhammad Ali Abid, Salvatore Esposito, Pablo Cavagnaro, Domenico Carputo, Massimo Iorizzo
Summary: The R2R3 MYB gene DcMYB11 is associated with petiole anthocyanin pigmentation in carrot, and a putative enhancer sequence that increases DcMYB11 activity was discovered. This study provides novel insight into the molecular mechanisms controlling anthocyanin accumulation in carrot and expands our knowledge on cis-regulatory sequences in plants.
Article
Biochemistry & Molecular Biology
Joo Tae Hwang, Hye Jin Kim, Jin Ah Ryuk, Dong Ho Jung, Byoung Seob Ko
Summary: This study explored the medicinal value of carrot leaves and isolated six flavone glycosides from them. A method using NMR and HPLC/UV was developed for their quantitation. Enzymatic deglycosylation of the flavone glycosides was assessed, and the enzyme-treated carrot leaves showed higher inhibitory effects on TNF-a and IL-2 expression. These findings highlight the importance of carrot leaves and provide baseline data for commercial development.
Article
Horticulture
Arthur Domblides, Elena Domblides
Summary: The biodiversity of carrot is important for human diet as it provides useful and necessary components. Eight carrot genotypes with different root colors were distinguished using amplified fragment length polymorphism (AFLP) markers. Three sets of primer pairs produced 92 loci, including 60 polymorphic ones. The genetic distances between the genotypes were calculated using Nei's coefficient. The AFLP method can be used for genetic diversity assessment in carrot breeding accessions with slight modifications including gel staining.
Article
Plant Sciences
Maria Doroteia Campos, Catarina Campos, Amaia Nogales, Helia Cardoso
Summary: The experiment revealed that DcAOX2a plays a dual role in carrot tap root secondary growth and cold stress response, while DcAOX1 shows less correlation. Cold stress generally up-regulated DcAOX2a without consistent effects on DcAOX1, indicating a potential key role of DcAOX2a in the response to stress. The identification of cis-acting regulatory elements at the promoters of both genes showed putative sequences involved in cold stress responsiveness and growth, with DcAOX2a promoter presenting more CAREs related to hormonal pathways than DcAOX1.
Article
Biochemistry & Molecular Biology
Claude Emmanuel Koutouan, Valerie Le Clerc, Anita Suel, Latifa Hamama, Patricia Claudel, David Halter, Raymonde Baltenweck, Philippe Hugueney, Jean-Francois Chich, Sitti Anlati Moussa, Clementine Champlain, Sebastien Huet, Linda Voisine, Sandra Pelletier, Sandrine Balzergue, Wilfried Chevalier, Emmanuel Geoffriau, Mathilde Briard
Summary: Alternaria leaf blight (ALB) is a destructive foliar disease of carrots caused by Alternaria dauci. Some carrot genotypes show partial resistance to ALB and resistance Quantitative Trait Loci (rQTL) have been identified. Metabolic and rQTL co-localization study revealed the potential involvement of specific terpenes, including camphene, alpha-pinene, alpha-bisabolene, beta-cubebene, caryophyllene, germacrene D, and alpha-humulene, in carrot resistance against ALB. Genomic and transcriptomic analyses identified differentially expressed terpene-related genes, including five terpene synthases and twenty transcription factors, in the co-localization regions between a resistant and a susceptible carrot genotype. Furthermore, alpha-humulene and caryophyllene displayed significant inhibition of mycelial growth.
Article
Chemistry, Multidisciplinary
Nicolas Gillaizeau-Simonian, Etienne Barde, Amandine Guerinot, Janine Cossy
Summary: A cobalt-catalyzed reaction method with high chemoselectivity was developed for the preparation of alpha-aryl amides with various functional groups. This method was successfully applied to the synthesis of alkaloids. Mechanistic studies suggest a radical process.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Review
Chemistry, Medicinal
Guillaume Masson, Domingo Gomez Pardo, Janine Cossy
Summary: This microreview focuses on the nucleophilic ring-opening of azetidiniums with various substitution patterns at C2, C3, and C4. The reactions often exhibit stereoselectivity and regioselectivity, leading to functionalized linear amines. Experimental selectivities correlated with Density Functional Theory (DFT) calculations provide insights into the factors governing regioselectivity.
Article
Chemistry, Organic
Andrea Zanetti, Geoffrey Schwertz, Marllon Nascimento de Oliveira, Mario Andres Gomez Fernandez, Zacharias Amara, Janine Cossy
Summary: A regioselective Pd-catalyzed allylic oxidation of amorphadiene, a key precursor to artemisinin, is described, which can substitute P450 enzymes in artemisinin biosynthesis and enhance the value of waste in the industrial semisynthetic process.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Chemistry, Organic
Mario Andres Gomez Fernandez, Marllon Nascimento de Oliveira, Andrea Zanetti, Geoffrey Schwertz, Janine Cossy, Zacharias Amara
Summary: A new method for the preparation of artemisinin was reported based on selective photochemical hydrothiolation of amorphadiene. Two distinctive activation pathways were discovered under solvent-free conditions or using a photocatalyst promoting H-abstraction. The key intermediate dihydroartemisinic aldehyde in the synthesis of artemisinin was obtained through chemoselective oxidation of the photochemically generated thioether followed by Pummerer rearrangement.
Review
Chemistry, Physical
Youssef Nassar, Fabien Rodier, Vincent Ferey, Janine Cossy
Summary: This review discusses the scope and limitations of stereoselective transformation of ketones to substituted olefins using first-row transition metal-catalyzed cross-couplings with various Grignard and zinc reagents.
Article
Chemistry, Multidisciplinary
Peter Polak, Janine Cossy
Summary: Good yields of 2-C-glycals and 2-C-ribals were achieved through a nickel-catalyzed cross-coupling reaction between 2-iodoglycals and 2-iodoribals using Grignard reagents. The synthesized compounds were further transformed into 2-C-2-deoxyglycosides, 2-C-diglycosides, and 2'-C-2'-deoxynucleosides. This method was successfully applied to the synthesis of a 2-chloroadenine 2'-deoxyribonucleoside, an important analogue of cladribine and clofarabine used in the treatment of multiple sclerosis and hairy cell leukemia.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Chemistry, Organic
Tomas J. Saiegh, Christophe Meyer, Janine Cossy
Summary: Hydroxamates derived from 3-sulfolene-3-carboxylic acid can undergo intra- and intermolecular rhodium(III)-catalyzed heteroannulations, involving C(sp(2))-H bond activation. These reactions provide a direct route to diversely substituted pyridones and pyridines fused to a sulfolene ring. The subsequent cheletropic elimination under microwave irradiation leads to the generation of pyridine ortho-quinodimethanes.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Chemistry, Organic
Thomas Abegg, Janine Cossy, Christophe Meyer
Summary: cis-1,2-Dialkenylcyclopropanes with a vinyl azide group were generated by Knoevenagel condensations and underwent cascade Cope and Winstein [3,3]-sigmatropic rearrangements under mild conditions. This reaction sequence allows for the synthesis of diversely substituted 1,4-cycloheptadienes with a secondary allylic azide containing up to three stereocenters.
Article
Chemistry, Organic
Peter Polak, Janine Cossy
Summary: This study reports the synthesis of 2-C-substituted 5-deoxyglucals from D-ribose using a nickel-catalyzed cross-coupling reaction. The obtained compounds were further transformed into 2-C-substituted 5-deoxyglycosides and 2-C-substituted 5-deoxynucleosides. The work also involves the reassignment of previously published structures.
Article
Chemistry, Organic
Janine Cossy
Summary: Due to advancements in techniques and technologies, biocatalysts are increasingly important in the field of chemistry. They have transitioned from being used on a laboratory scale to an industrial scale, allowing for efficient synthesis of complex molecules. Biocatalysis opens new areas of research and addresses challenges that traditional chemistry struggles with in terms of chemoselectivity and efficiency.
Article
Chemistry, Organic
Paul C. Ruer, Youssef Nassar, Peter Polak, Janine Cossy
Summary: Five-membered exo-glycals were synthesized from six-membered 2-iodo-endo-glycals through a metal/halogen exchange/ring-opening sequence followed by a cyclization catalyzed by Ag2CO3.
JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Elias Abedelnour, Stephanie Ognier, Olivier Venier, Laurent Schio, Michael Tatoulian, Janine Cossy
Summary: This study presents the direct synthesis of trifluoromethyl N,N-aminals from nitrogen containing heterocycles using argon plasma in a continuous flow microreactor without any additives or metal catalysts. The transformation of these compounds to N-trifluoroethyl amines is also reported.
CHEMICAL COMMUNICATIONS
(2023)
Review
Chemistry, Organic
Tomohiro Yasukawa, Katja S. Haheim, Janine Cossy
Summary: This article introduces the synthesis of 1,3-disubstituted bicyclo[1.1.1]pentanes (BCPs) by forming a C-C bond through cross-coupling reactions using transition metal catalysts. Two main strategies are described to obtain these compounds from nucleophilic BCPs or electrophilic BCPs, including relevant mechanisms.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
Review
Chemistry, Organic
Janine Cossy, Peter Polak, Paul C. Ruer
Summary: This review focuses on the incorporation of a cyclobutyl substituent in molecules by transition metal-catalyzed cross-coupling, specifically the formation of C-C bonds. Three main strategies are discussed, involving electrophilic or nucleophilic cyclobutane derivatives.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Elias Abedelnour, Stephanie Ognier, Mengxue Zhang, Laurent Schio, Olivier Venier, Janine Cossy, Michael Tatoulian
Summary: The direct N-acylation of amines by esters in a microreactor using argon plasma is presented, resulting in high yields of amides.
CHEMICAL COMMUNICATIONS
(2022)
