Article
Multidisciplinary Sciences
Brian Josephson, Charlie Fehl, Patrick G. Isenegger, Simon Nadal, Tom H. Wright, Adeline W. J. Poh, Ben J. Bower, Andrew M. Giltrap, Lifu Chen, Christopher Batchelor-McAuley, Grace Roper, Oluwatobi Arisa, Jeroen B. I. Sap, Akane Kawamura, Andrew J. Baldwin, Shabaz Mohammed, Richard G. Compton, Veronique Gouverneur, Benjamin G. Davis
Article
Microbiology
Suzanne Pickering, Gilberto Betancor, Rui Pedro Galao, Blair Merrick, Adrian W. Signell, Harry D. Wilson, Mark Tan Kia Ik, Jeffrey Seow, Carl Graham, Sam Acors, Neophytos Kouphou, Kathryn J. A. Steel, Oliver Hemmings, Amita Patel, Gaia Nebbia, Sam Douthwaite, Lorcan O'Connell, Jakub Luptak, Laura E. McCoy, Philip Brouwer, Marit J. van Gils, Rogier W. Sanders, Rocio Martinez Nunez, Karen Bisnauthsing, Geraldine O'Hara, Eithne MacMahon, Rahul Batra, Michael H. Malim, Stuart J. D. Neil, Katie J. Doores, Jonathan D. Edgeworth
Article
Microbiology
Jeffrey Seow, Carl Graham, Blair Merrick, Sam Acors, Suzanne Pickering, Kathryn J. A. Steel, Oliver Hemmings, Aoife O'Byrne, Neophytos Kouphou, Rui Pedro Galao, Gilberto Betancor, Harry D. Wilson, Adrian W. Signell, Helena Winstone, Claire Kerridge, Isabella Huettner, Jose M. Jimenez-Guardeno, Maria Jose Lista, Nigel Temperton, Luke B. Snell, Karen Bisnauthsing, Amelia Moore, Adrian Green, Lauren Martinez, Brielle Stokes, Johanna Honey, Alba Izquierdo-Barras, Gill Arbane, Amita Patel, Mark Kia Ik Tan, Lorcan O'Connell, Geraldine O'Hara, Eithne MacMahon, Sam Douthwaite, Gaia Nebbia, Rahul Batra, Rocio Martinez-Nunez, Manu Shankar-Hari, Jonathan D. Edgeworth, Stuart J. D. Neil, Michael H. Malim, Katie J. Doores
NATURE MICROBIOLOGY
(2020)
Article
Chemistry, Multidisciplinary
Mateusz Imiolek, Patrick G. Isenegger, Wai-Lung Ng, Aziz Khan, Veronique Gouverneur, Benjamin G. Davis
Summary: This study presents a novel method for carbonylation of native protein residues through reductive photoredox catalysis, resulting in a new unnatural protein residue CfW with selective modifications on Trp residues. CfW not only shows effectiveness in bioconjugation, ligation, and labeling reactions, but also exhibits a strong red-shift in its absorption and fluorescent emission maxima.
ACS CENTRAL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Kuan-Yu Lai, Sebastien R. G. Galan, Yibo Zeng, Tianhui Hina Zhou, Chang He, Ritu Raj, Jitka Riedl, Shi Liu, K. Phin Chooi, Neha Garg, Min Zeng, Lyn H. Jones, Graham J. Hutchings, Shabaz Mohammed, Satish K. Nair, Jie Chen, Benjamin G. Davis, Wilfred A. van der Donk
Summary: LanCL catalyzes the addition of glutathione to Dha/Dhb in proteins, driving irreversible C-glutathionylation to repair damages in proteins, indicating its physiological importance in removing reactive electrophiles and dysregulatory effects from the proteome.
Editorial Material
Chemistry, Multidisciplinary
Benjamin G. Davis
ACS CENTRAL SCIENCE
(2021)
Correction
Biochemistry & Molecular Biology
Tim A. Mollner, Patrick G. Isenegger, Brian Josephson, Charles Buchanan, Lukas Lercher, Daniel Oehlrich, D. Flemming Hansen, Shabaz Mohammed, Andrew J. Baldwin, Veronique Gouverneur, Benjamin G. Davis
NATURE CHEMICAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tim A. Mollner, Patrick G. Isenegger, Brian Josephson, Charles Buchanan, Lukas Lercher, Daniel Oehlrich, D. Flemming Hansen, Shabaz Mohammed, Andrew J. Baldwin, Veronique Gouverneur, Benjamin G. Davis
Summary: Post-translational formation of boronoalanine in proteins through C beta-B gamma bond allows for mild, direct, and site-selective access, enabling tunable interactions and de novo function.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Isidro Cobo, M. Isabel Matheu, Sergio Castillon, Benjamin G. Davis, Omar Boutureira
Summary: This study investigates the scope and limitations of common bioconjugation techniques for generating well-defined glycodendriproteins, as synthetic functional mimics of glycoproteins. A diverse reactive platform was designed using recombinant proteins containing non-canonical amino acids, resulting in the successful synthesis of multivalent carbohydrate moieties attached to proteins.
Article
Multidisciplinary Sciences
Tim A. Mollner, Andrew M. Giltrap, Yibo Zeng, Yana Demyanenko, Charles Buchanan, Daniel Oehlrich, Andrew J. Baldwin, Daniel C. Anthony, Shabaz Mohammed, Benjamin G. Davis
Summary: Biomolecule environments have the potential to enhance chemistries and mediate self-modification. This study demonstrates a system that combines protein-enhanced chemistry with tag modification to enable synthetic reductive protein chemistries. This method allows for simple and site-selective cleavage of proteins under aqueous conditions and in cell lysates, making it a valuable tool for precise chemical proteolysis and protein modification.
Article
Multidisciplinary Sciences
Charles J. Buchanan, Ben Gaunt, Peter J. Harrison, Yun Yang, Jiwei Liu, Aziz Khan, Andrew M. Giltrap, Audrey Le Bas, Philip N. Ward, Kapil Gupta, Maud Dumoux, Tiong Kit Tan, Lisa Schimaski, Sergio Daga, Nicola Picchiotti, Margherita Baldassarri, Elisa Benetti, Chiara Fallerini, Francesca Fava, Annarita Giliberti, Panagiotis Koukos, Matthew J. Davy, Abirami Lakshminarayanan, Xiaochao Xue, Georgios Papadakis, Lachlan P. Deimel, Virginia Casablancas-Antras, Timothy D. W. Claridge, Alexandre M. J. J. Bonvin, Quentin J. Sattentau, Simone Furini, Marco Gori, Jiandong Huo, Raymond J. Owens, Christiane Schaffitzel, Imre Berger, Alessandra Renieri, James H. Naismith, Andrew J. Baldwin, Benjamin G. Davis
Summary: This study utilizes universal saturation transfer analysis to reveal the interaction between the novel coronavirus and glycan compounds, and identifies protein mutations related to pathogenicity and zoonosis.
Article
Biochemical Research Methods
Patrick G. Isenegger, Brian Josephson, Ben Gaunt, Matthew J. Davy, Veronique Gouverneur, Andrew J. Baldwin, Benjamin G. Davis
Summary: This article describes a robust, mild and fast method for the posttranslational, site-directed fluorination of protein sidechains, which can be detected by F-19-based magnetic resonance methods. This chemical editing method is stable, mild, fast and highly efficient, and can be used by non-experts to prepare fluorinated proteins within 24 hours without the need for bespoke biological systems.
Article
Chemistry, Multidisciplinary
Benjamin G. Davis, Xuanxiao Chen, Brian Josephson
Summary: Methods to directly modify proteins post-translationally have potential for creating and studying protein post-translational modifications. Recent developments in single electron/radical chemistry have allowed for site-specific editing of proteins. This review gives an overview of current methods that install such edits, focusing on radical C-C bond formation and its compatibility with proteins and cells, as well as the novel applications afforded by these methods. Despite technical challenges, radical C-C bond formation on proteins is a promising and rapidly growing field in chemical biology.
ACS CENTRAL SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Xia-Ping Fu, Yizhi Yuan, Ajay Jha, Nikita Levin, Jack Ren, Dimitrios Mamalis, Shabaz Mohammed, Benjamin G. Davis, Andrew M. Giltrap
Summary: Chemical post-translational methods allow editing of proteins without genetic intervention. A light-mediated desulfurative method can generate site-selectively edited proteins with full retention of native stereochemistry from a natural amino acid precursor. This methodology has great potential in exploring protein side-chain diversity and function and constructing useful bioconjugates.
ACS CENTRAL SCIENCE
(2023)
Article
Medicine, Research & Experimental
Anna Jeffery-Smith, Alice R. Burton, Sabela Lens, Chloe Rees-Spear, Jessica Davies, Monika Patel, Robin Gopal, Luke Muir, Felicity Aiano, Katie J. Doores, J. Yimmy Chow, Shamez N. Ladhani, Maria Zambon, Laura E. McCoy, Mala K. Maini
Summary: Memory B cells specific to SARS-CoV-2 can persist beyond the loss of neutralizing antibodies and provide a reserve immunity. However, activated MBCs are expanded in the elderly, indicating ongoing antigenic stimulation or inflammation. Some individuals who have lost neutralizing antibodies still exhibit MBCs and functional immune response to SARS-CoV-2, but this is significantly impaired in the elderly.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Chemistry, Organic
Faiza Diaba, Gisela Trenchs
Summary: The first violet light-mediated synthesis of gamma- and delta-lactams from N-alkenyl trichloroacetamides is reported in this paper. The reactions are conducted in tetrahydrofuran or 2-methyltetrahydrofuran as the sole solvent without catalysts or additives, under non-anhydrous conditions in an air atmosphere where the solvent serves as the radical initiator.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
Article
Chemistry, Organic
Feroze Hussain, Sajjad Ahmed, Ashiq Hussain Padder, Qazi Naveed Ahmed
Summary: This study reports a novel and efficient one-pot synthesis method for mixed phosphorotrithioates, which does not require supplementary additives and shows broad applicability.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
Article
Chemistry, Organic
Hyunjin Oh, Ikyon Kim
Summary: A new 1,2,4-triazole-pyrrolo[1,2-a]pyrazine hybrid system, 6-acylpyrrolo[1,2-a][1,2,4]triazolo[5,1-c]pyrazine, was synthesized using a catalyst-free method. This method involved sequential exposure of pyrrole-2-carbonitrile-derived substrates to DMF-DMA and acyl hydrazide, resulting in the formation of acylated pyrazine and 1,2,4-triazole rings, enabling the installation of various substituents at specific positions on the core skeleton.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
Article
Chemistry, Organic
Ming Yan, Si-fan Wang, Yong-po Zhang, Jin-zhong Zhao, Zhuo Tang, Guang-xun Li
Summary: Here we developed an efficient photocatalytic approach for the convenient preparation of sulfinamides. The reaction allows for the gram-scale preparation of sulfinamides and the one-pot synthesis of various sulfonyl amides.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
Article
Chemistry, Organic
Farzaneh Bandehali-Naeini, Zahra Tanbakouchian, Noushin Farajinia-Lehi, Nicolas Mayer, Morteza Shiri, Martin Breugst
Summary: Two tandem catalytic systems were developed for the synthesis of novel 3,4-disubstituted maleimides using the same Ugi adducts. Different maleimide structures can be synthesized using either Pd or Cu catalysis.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
Article
Chemistry, Organic
Tanya Raghava, Anjan Chattopadhyay, Subhadeep Banerjee, Nivedita Sarkar
Summary: Amine substitution of two ortho fluorine atoms of tetrafluoroterephthalonitrile through SNAr chemistry is easily achievable. But further fluorine substitution is only possible under forcing conditions, yielding valuable fluorophores for bioimaging.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
Review
Chemistry, Organic
Anju Chadha, Santosh Kumar Padhi, Selvaraj Stella, Sowmyalakshmi Venkataraman, Thangavelu Saravanan
Summary: Alcohol dehydrogenases are enzymes that use cofactors for oxidation or reduction reactions of alcohols or carbonyl compounds. They are utilized in green chemistry and have applications in the production of pharmaceuticals. Recombinant enzymes have solved the challenge of producing purified enzymes in large quantities. Engineered alcohol dehydrogenases have been used in asymmetric synthesis in industry. Various methods have been established for regenerating expensive cofactors to make the enzymatic process more efficient and economically viable.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
