Article
Multidisciplinary Sciences
Takumi Hiyoshi, Hisanori Domon, Tomoki Maekawa, Hikaru Tamura, Toshihito Isono, Satoru Hirayama, Karin Sasagawa, Fumio Takizawa, Koichi Tabeta, Yutaka Terao
Summary: This study reveals the mechanisms by which neutrophil elastase (NE) exacerbates periodontitis. NE induces increased neutrophil recruitment and activity in the periodontal tissue, leading to worsened periodontal bone loss. Administration of an NE inhibitor reduces NE activity and attenuates periodontal bone loss. NE also promotes bacterial invasion in periodontal tissues by cleaving cell adhesion molecules and disrupting the gingival epithelial barrier.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Javier Fernandez, Constanza Jimenez, Dafna Benadof, Paulina Morales, Jessica Astorga, Felipe Caceres, Marcela Hernandez, Alejandra Fernandez, Fernando Valenzuela
Summary: The study aimed to evaluate the levels of MMP-2 and MMP-9 in gingival crevicular fluid as novel biomarkers for rosacea. The results showed a significant increase in MMP-9 concentration in the GCF of patients with rosacea compared to healthy patients. MMP-9 levels can be used as a diagnostic biomarker for rosacea.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Luigi Nibali, Abish Stephen, Eleni Hagi-Pavli, Robert Allaker, Antonino Di Pino, Valentina Terranova, Marcella Pisano, Salvatore Di Marca, Viviana Ferrara, Roberto Scicali, Mauro Giordano, Francesco Purrello, Nikolaos Donos, Lorenzo Malatino
Summary: The study suggests that GCF markers in patients with metabolic syndrome may be associated with concomitant periodontitis, particularly the levels of markers such as Aggrecan, IL-6, and IL-8 are correlated with the severity of the disease.
JOURNAL OF DENTISTRY
(2022)
Article
Health Care Sciences & Services
Hammam Ibrahim Fageeh, Hytham N. Fageeh, Shankargouda Patil
Summary: Periodontitis is an inflammatory condition caused by pathogenic bacteria in dental plaque. Macrophages derived from monocytes play a dual role in periodontitis, presenting antigens to lymphocytes while also contributing to periodontal destruction. The study found that GCF samples from periodontitis patients have higher levels of specific cytokines, and that treatment with these samples can induce monocytes to differentiate into destructive macrophages, affecting the phenotype and function of resident cells in the periodontium.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Dentistry, Oral Surgery & Medicine
Jie Zhang, Wenjing Li, Hongye Lu, Ruifang Lu, Yalin Zhan, Huanxin Meng
Summary: The level of platelets in the GCF of patients with periodontitis is higher than that of healthy individuals. Platelets can interact with periodontal pathogens and neutrophils, and promote the formation of NETs.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2022)
Article
Microbiology
Simran Preet Bhalla, Ann Maria Shaju, Carlos Marcelo da Silva Figueredo, Leticia Algarves Miranda
Summary: C5a levels were significantly increased in both gingival crevicular fluid and saliva of patients with periodontal disease, indicating a potential role of this molecule in the pathogenesis of the disease.
Article
Chemistry, Multidisciplinary
Alina-Ramona Dimofte, Dorin Nicolae Gheorghe, Dora Maria Popescu, Ioana Mitrut, Petre Costin Marasescu, Horia Octavian Manolea, Mihail Virgil Boldeanu, Petra Surlin
Summary: This study evaluated the influence of metal-ceramic crowns on periodontal parameters and inflammatory marker levels. The results showed that metal-ceramic crowns can cause changes in periodontal clinical parameters and are associated with inflammatory marker levels.
APPLIED SCIENCES-BASEL
(2023)
Article
Dentistry, Oral Surgery & Medicine
Bardia Vadiati Saberi, Farouq Yousefi, Tooba Yousefi, Durdi Qujeq
Summary: This study determined the correlation between mucin and alpha-amylase protein levels in gingival crevicular fluid (GCF) with chronic periodontitis. The results showed that the levels of both markers were higher in patients with chronic periodontitis compared to successfully recovered patients, suggesting that they could be used to determine the activity of periodontal disease.
Article
Dentistry, Oral Surgery & Medicine
Rafael Simoes Goncalves, Polliana Mendes Candia Scaffa, Mirela Sanae Shinohara, Paulo Roberto Marao de Andrade Carvalho, Marilia Afonso Rabelo Buzalaf, Ticiane Cestari Fagundes
Summary: Different restorative techniques for non-carious cervical lesions (NCCLs) showed similar performance after 2 years with initial marginal defect alterations. MMP-2 reestablished its initial levels after 2 years, while MMP-9 had few alterations over time in crevicular fluid.
CLINICAL ORAL INVESTIGATIONS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Beral Afacan, Harika Atmaca Ilhan, Timur Kose, Gulnur Emingil
Summary: This study aims to evaluate the levels of GCF Galectin-3 and IL-1 beta in different grades of stage 3 periodontitis and investigate their efficiency in discriminating periodontal diseases. ELISA was used to measure the total amounts of GCF Galectin-3 and IL-1 beta in 80 individuals. The results showed that GCF Galectin-3 is associated with the pathogenesis of periodontal diseases and has high diagnostic performance.
CLINICAL ORAL INVESTIGATIONS
(2023)
Article
Biochemical Research Methods
Xin Zhang, Xiaoping Xiao, Yue Mu, Yao Ran Liu, Xinxin Lin, Qian Li, Wei Sun
Summary: This study investigated the influence of tooth site, sex, and age on the normal GCF proteome in periodontal health. Results showed that tooth site had a small impact, while sex and age were important factors affecting the GCF proteome, with sex having a significant influence on young adults.
JOURNAL OF PROTEOMICS
(2022)
Article
Biology
Hytham N. Fageeh, Hammam Ibrahim Fageeh, Samar Saeed Khan, Prabhadevi C. Maganur, Nishant Vyas, Vikrant R. Patil, A. Thirumal Raj, Hosam Ali Baeshen, Shankargouda Patil
Summary: This study aimed to characterize monocytes isolated from the gingival crevicular fluid (GCF) in patients with periodontitis, evaluating cytokine levels, gene expression, and secreted cytokines and chemokines. CD14+ monocytes from GCF secreted higher levels of CCL2 and expressed genes responsible for monocyte migration, indicating their role in the inflammatory microenvironment of periodontitis.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Dentistry, Oral Surgery & Medicine
Zeliha Guney, Sivge Kurgan, Canan Onder, Cavid Mammadov, Muhittin A. Serdar, Meral Gunhan
Summary: This study aimed to evaluate the levels of ADMA, SDMA, and IL-1 beta in GCF from periodontitis patients. The results showed that the levels of IL-1 beta, ADMA, and SDMA in GCF were significantly higher in periodontitis patients compared to the control group, and they were positively correlated with clinical periodontal parameters. It suggests that ADMA and SDMA may be involved in the pathogenesis of periodontal disease.
JOURNAL OF PERIODONTAL RESEARCH
(2023)
Article
Biology
Ashley L. L. Gard, Rebeccah J. J. Luu, Ryan Maloney, Madeline H. H. Cooper, Brian P. P. Cain, Hesham Azizgolshani, Brett C. C. Isenberg, Jeffrey T. T. Borenstein, Jane Ong, Joseph L. L. Charest, Else M. M. Vedula
Summary: A microfluidic model of human gingival tissue allows for the study of disease progression and evaluation of therapeutic agents for gum disease. This model demonstrates physiological tissue structure and response to inflammatory triggers, providing a platform for identification of new therapeutic targets.
COMMUNICATIONS BIOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Angela Gusiyska, Elena Dyulgerova
Summary: Knowledge of dental anatomy and three-dimensional principles is necessary for effective root canal treatment. The selection of obturation techniques depends on the canal's morphology and therapeutic goals. Bioceramic sealers are the latest development in endodontic sealing materials.
APPLIED SCIENCES-BASEL
(2023)
Article
Dentistry, Oral Surgery & Medicine
James Rudolph Collins, Kenia Veras, Marcela Hernandez, Wei Hou, Houlin Hong, Georgios E. Romanos
Summary: This study compared the anti-inflammatory efficacy of sodium chloride and 0.12% chlorhexidine mouth rinses in patients undergoing minimal invasive periodontal surgery. The results indicated that sodium chloride mouth rinse had similar anti-inflammatory properties to chlorhexidine mouth rinse postoperatively and could be used regularly after periodontal surgical procedures.
CLINICAL ORAL INVESTIGATIONS
(2021)
Review
Biochemistry & Molecular Biology
Sven Niklander, Maria Jose Bordagaray, Alejandra Fernandez, Marcela Hernandez
Summary: VEGFs are crucial regulators of angiogenesis, with important roles in oral cancer and destructive periodontal inflammatory diseases, offering potential translational applications as biomarkers and therapeutic targets.
Article
Dentistry, Oral Surgery & Medicine
Luis A. Cordova, Montserrat Reyes, Ruben Soto, Marcela Hernandez, Juan E. Cortes
Summary: Analysis of samples from patients diagnosed with TMJ ankylosis revealed an association between the presence of mature/lamellar bone and osteoclasts, as well as a lack of association between previous trauma or infection history and the histological type of ankylosis. Inflammatory or infectious histological presence also did not correlate with the type of ankylosis. Tissues from retrieved TMJ ankyloses displayed aberrant ossification and reduced focal bone resorption, indicating a dysregulated healing response.
JOURNAL OF CRANIO-MAXILLOFACIAL SURGERY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Constanza Jimenez, Mauricio Garrido, Pirkko Pussinen, Maria Jose Bordagaray, Alejandra Fernandez, Claudia Vega, Alejandra Chaparro, Anilei Hoare, Marcela Hernandez
Summary: The study found that the detection rates of endodontic Porphyromonas endodontalis and Porphyromonas gingivalis in apical periodontitis patients were 33.3% and 22.9% respectively. AP patients had higher levels of serum anti-P. endodontalis and anti-P. gingivalis IgG compared to controls. Presence of endodontic P. endodontalis was associated with higher endotoxemia, while anti-P. endodontalis IgG seemed to be protective against low-grade systemic inflammation.
CLINICAL ORAL INVESTIGATIONS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Alejandra Fernandez, Jessica Astorga, Maria Jose Bordagaray, Maria Jesus Lira, Peter J. Gebicke-Haerter, Marcela Hernandez
Summary: This study explores the methylation pattern and its impact on gene transcription of the TLR9 gene in chronic periapical inflammation. The results show that demethylation of certain CpG sites in the TLR9 promoter region enhances transcriptional activity. Additionally, age and smoking were found to affect the overall methylation status of the gene.
INTERNATIONAL ENDODONTIC JOURNAL
(2022)
Article
Engineering, Chemical
Taise Alessandra Hanzen, Mario Felipe Gutierrez, Thalita de Paris Matos, Alexandra Mara de Paula, Fabiana Suelen Figueredo de Siqueira, Andres Felipe Millan Cardenas, Andres Viteri-Garcia, Marcela Hernandez, Alessandro D. Loguercio, Alessandra Reis
Summary: This study evaluated the inhibition of matrix metalloproteases by copper nanoparticles in a universal adhesive system. The results showed that copper nanoparticles not only exhibited antimicrobial activity, but also maintained stable bond strength and nanoleakage values, particularly in eroded dentin.
INTERNATIONAL JOURNAL OF ADHESION AND ADHESIVES
(2022)
Article
Immunology
Maria Jose Bordagaray, Alejandra Fernandez, Jessica Astorga, Mauricio Garrido, Patricia Hernandez, Alejandra Chaparro, Maria Jesus Lira, Peter Gebicke-Haerter, Marcela Hernandez
Summary: PBMCs from individuals with AP exhibit a proinflammatory phenotype and upregulation of TLR2, associated with CpG site methylation in the promoter region of the TLR2 gene.
FRONTIERS IN IMMUNOLOGY
(2022)
Editorial Material
Immunology
Marcela Hernandez, Marcia Pinto Alves Mayer, Julien Santi-Rocca
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
J. Astorga, M. Hernandez, D. Bravo, A. Hoare
Summary: This study evaluated the effectiveness of two PCR primers in identifying P. endodontalis in apical periodontitis, and only one primer set showed specificity for P. endodontalis clones both in silico and in vitro.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Pablo Veloso, Alejandra Fernandez, Jessica Astorga, David Gonzalez-Quintanilla, Alfredo Castro, Alejandro Escobar, Anilei Hoare, Marcela Hernandez
Summary: The study found that Pg LPS can induce a macrophage M1 phenotype (pro-inflammatory) while Pe LPS does not elicit a significant response. Additionally, the expressions of TLR2 and TLR4 were not modified by Pg or Pe LPS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Javier Fernandez, Constanza Jimenez, Dafna Benadof, Paulina Morales, Jessica Astorga, Felipe Caceres, Marcela Hernandez, Alejandra Fernandez, Fernando Valenzuela
Summary: The study aimed to evaluate the levels of MMP-2 and MMP-9 in gingival crevicular fluid as novel biomarkers for rosacea. The results showed a significant increase in MMP-9 concentration in the GCF of patients with rosacea compared to healthy patients. MMP-9 levels can be used as a diagnostic biomarker for rosacea.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Alejandra Chaparro, Mauricio Lozano, Dominique Gaedechens, Carolina Lopez, Daniela Albers, Marcela Hernandez, Andres Pascual, Jose Nart, Carlos E. Irarrazabal
Summary: This study explores the relationship between miRNAs and target genes related to inflammation and hypoxia in periodontitis and gingival mesenchymal stem cells (GMSCs). The expression of miRNA-20a, miRNA-30e, and miRNA-93 increased in periodontitis patients, while NFAT5 mRNA expression was decreased. Under hypoxic conditions, GMSCs upregulated HIF-1α and miRNA-20a expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Alejandra Fernandez, Daniela Herrera, Anilei Hoare, Marcela Hernandez, Vicente A. A. Torres
Summary: This study aimed to evaluate the angiogenesis-related effects induced by lipopolysaccharide (LPS) from Porphyromonas endodontalis and Porphyromonas gingivalis in vitro via TLR2 and TLR4. The results showed that P. endodontalis LPS stimulated endothelial cell migration and tube formation through TLR2 and TLR4 activation, while P. gingivalis LPS did not have these effects. Moreover, both LPS induced IL-6 and VEGF-A synthesis in endothelial cells.
INTERNATIONAL ENDODONTIC JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Constanza Jimenez, Javier Fernandez, Marcela Aroca, Maria Jose Bordagaray, Elizabeth Pellegrini, Javier Contador, Marcela Hernandez, Fernando Valenzuela, Alejandra Fernandez
Summary: This case-control study found a link between atopic dermatitis (AD) and periodontitis, with Th2-derived cytokines playing a key role in the development of both diseases. The study also found higher levels of IL-31 and TSLP in AD subjects, while severe periodontitis was associated with lower levels of IL-31.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Dentistry, Oral Surgery & Medicine
Nathalie Derderian, Vanesa Pereira-Prado, Marcela Hernandez, Mario Isiordia-Espinoza, Miguel Arocena, Rogelio Gonzalez-Gonzalez, Omar Tremillo-Maldonado, Marco Meleti, Nelly Molina-Frechero, Ronell Bologna-Molina
Summary: This study analyzed the expression of Ep-CAM protein in ameloblastoma and tooth germ, and found that most tooth germs expressed this protein, while the expression was less in ameloblastoma. Further studies with different experimental strategies are suggested to clarify the biological significance of this finding.
MEDICINA ORAL PATOLOGIA ORAL Y CIRUGIA BUCAL
(2022)
