Journal
ORAL DISEASES
Volume 18, Issue 2, Pages 169-177Publisher
WILEY
DOI: 10.1111/j.1601-0825.2011.01860.x
Keywords
CD133; CD44; Tca8113 cell line; tongue squamous cell carcinoma
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Funding
- Natural Science Foundation of China [81000679]
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OBJECTIVES: To isolate the CD133+CD44+ cells from human tongue squamous cell carcinoma (TSCC) Tca8113 cell line and investigate biological characteristics of them. MATERIALS AND METHODS: Immunomagnetic microbeads were applied to sort the CD133+CD44+ cells. Flow cytometry was used to detect isolation purity. The proliferation, clone-formation efficiencies, invasion and migration, gene expressions, and tumor-formation abilities were analyzed among CD133+CD44+, CD133-CD44- and total population of cells. RESULTS: The average purities of CD133+ and CD44+ cells reached 97.3% and 98.7%, respectively. The proliferation of CD133+ CD44+ cells was significantly higher than the other two groups. The clone-forming efficiency of three groups was 70%, 8%, and 14%, respectively. The average invaded and migrated cell numbers of CD133+ CD44+ and total population cells were 132 and 36.2, 311.6, and 156.2, respectively. The expressions of Bcl2 and Sox2 in CD133+ CD44+ cells were significantly higher than those in total population cells. A total of 10 4 CD133+ CD44+ cells could form secondary tumors in nude mice, while the total population group needed 10 6 cells. CONCLUSIONS: The CD133+ CD44+ subpopulation cells possess stem-like characteristics. They appear to be the potential targets for future biology therapy of human TSCC. Oral Diseases (2012) 18, 169-177
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