Journal
OPTICS LETTERS
Volume 38, Issue 15, Pages 2683-2686Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.38.002683
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Funding
- National Institutes of Health [DP1 EB016986, R01 EB008085, R01 CA134539, U54 CA136398, R01 EB010049, R01 CA157277, R01 CA159959]
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We developed random-access optical-resolution photoacoustic microscopy using a digital micromirror device. This system can rapidly scan arbitrarily shaped regions of interest within a 40 mu m x 40 mu m imaging area with a lateral resolution of 3.6 mu m. To identify a region of interest, a global structural image is first acquired, then the selected region is scanned. The random-access ability was demonstrated by imaging two static samples, a carbon fiber cross and a monolayer of red blood cells, with an acquisition rate up to 4 kHz. The system was then used to monitor blood flow in vivo in real time within user-selected capillaries in a mouse ear. By imaging only the capillary of interest, the frame rate was increased by up to 9.2 times. (C) 2013 Optical Society of America
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