Journal
OPTICS LETTERS
Volume 34, Issue 23, Pages 3668-3670Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.34.003668
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Funding
- National Institutes of Health (NIH) [P41-RR02594-18]
- National Science Foundation [DBI-0754339]
- Hamamatsu Corporation
- Samsung Fellowship
- Direct For Biological Sciences
- Div Of Biological Infrastructure [0754339] Funding Source: National Science Foundation
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We report a practical method for label-free quantification of specific molecules using spectroscopic imaging of sample-induced phase shifts. Diffraction phase microscopy equipped with various wavelengths of light source is used to record wavelength-dependent phase images. We first perform dispersion measurements on pure solutions of single molecular species present in the cells, such as albumin and hemoglobin (Hb). With this prior calibration of molecular specific dispersion, we demonstrate the extraction of Hb concentration from individual human red blood cells. The end point of this study is noninvasive monitoring of physiological states of intact living cells. (C) 2009 Optical Society of America
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