Journal
OPTICS LETTERS
Volume 33, Issue 7, Pages 714-716Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.33.000714
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Funding
- NIDCR NIH HHS [1-R21-DE016111-01A1] Funding Source: Medline
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A microscopy system has been constructed that is capable of simultaneously acquiring both traditional Raman spectra as well as angle-resolved elastic-scattering patterns using a single focused laser spot less than 10 mu m wide. The elastic-scattering signal was analyzed by generalized Lorenz-Mie theory, representing what we believe to be the first experimental validation of the theory's prediction of angular backscatter from single spheres. The microscope system exhibits 3 nm precision in predicting sphere diameters, while simultaneously yielding high-quality Raman signals. Applications to single cell analysis are envisioned. (C) 2008 Optical Society of America.
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