4.6 Article

Application of the T-matrix method to determine the structure of spheroidal cell nuclei with angle-resolved light scattering

Journal

OPTICS LETTERS
Volume 33, Issue 21, Pages 2452-2454

Publisher

OPTICAL SOC AMER
DOI: 10.1364/OL.33.002452

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Funding

  1. National Institutes of Health (NIH) [NCI R33CA109907]
  2. National Science Foundation (NSF) [BES 03-48204]

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We demonstrate an inverse light-scattering analysis procedure based on using the T-matrix method as a light-scattering model. We measure light scattered by in vitro cell monolayers using angle-resolved low-coherence interferometry (a/LCI) and compare the data to predictions of the T-matrix theory. The comparison yields measurements of the equal volume diameter and aspect ratio of the spheroid cell nuclei with accuracy comparable to quantitative image analysis of fixed and stained samples. These improvements represent a significant upgrade for the a/LCI technique, expanding both the range of tissue in which it is applicable and potentially increasing its value as a diagnostic tool. (C) 2008 Optical Society of America

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