Journal
OPTICS EXPRESS
Volume 22, Issue 1, Pages 1094-1104Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.22.001094
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Funding
- National Major Scientific Research Program of China [2011CB910401]
- Science Fund for Creative Research Group of China [61121004]
- National Natural Science Foundation of China [81201067, 81171376]
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Tissue optical clearing technique has shown great potential for enhancing the imaging depth and contrast of optical imaging modalities. However, the mechanism of optical clearing is still in controversy. In this manuscript, we combined photoacoustic microscopy with ultrasonography to monitor the dermic changes induced by optical clearing agents at different immersion time points. The measured parameters were correlated with the optical clearing process, and could be used to assess the optical clearing effect. Both in vitro and in vivo results demonstrated that photoacoustic microscopy and ultrasonography can potentially be used as a powerful tool in screening optical clearing agents and exploring the mechanism of optical clearing. (C)2014 Optical Society of America
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