Article
Reproductive Biology
Kyungjun Uh, Alayna Hay, Paula Chen, Emily Reese, Kiho Lee
Summary: Artificial oocyte activation is essential for the application of Assisted Reproductive Technology, and the ability to control the level of cytoplasmic zinc can facilitate the oocyte activation process.
BIOLOGY OF REPRODUCTION
(2022)
Review
Obstetrics & Gynecology
Bei Sun, John Yeh
Summary: This paper reviews the effects of calcium oscillatory patterns in oocytes and early embryo development, suggesting that abnormal calcium oscillations may be the primary mechanism for total fertilization failure (TFF). Studies also found strong associations between abnormal calcium oscillatory patterns and suboptimal developmental outcomes. Empirical use of artificial oocyte activation (AOA) methods has shown initial success in helping TFF patients.
REPRODUCTIVE SCIENCES
(2021)
Article
Obstetrics & Gynecology
Lei Jia, Panyu Chen, Wenlong Su, Shujing He, Yingchun Guo, Lei Zheng, Cong Fang, Xiaoyan Liang
Summary: This retrospective cohort study compared the fertilization rates in ICSI cycles treated with artificial oocyte activation (AOA) using 10 mu mol/l ionomycin or commercial A23187. The results showed that the fertilization rates were significantly higher in the ionomycin group compared to the A23187 group for all subgroups. Therefore, AOA with 10 mu mol/l ionomycin may be more effective in improving oocyte activation, especially in cases of sperm-related defects.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Agriculture, Dairy & Animal Science
Juan P. Cabeza, Juan Camera, Olinda Briski, Minerva Yauri Felipe, Daniel F. Salamone, Andres Gambini
Summary: This study investigates the use of a novel zinc chelator for activating bovine and porcine oocytes. The results show that the zinc chelator can successfully induce blastocyst development in both species, although developmental rates and embryo quality are compromised in bovine compared to calcium-induced embryos. On the contrary, the new concentration and incubation time of the zinc chelator improve developmental rates in porcine without affecting embryo quality. These findings contribute to understanding the role of zinc in oocyte activation and preimplantation embryo development across different mammalian species.
Review
Biochemistry & Molecular Biology
Mohammad Ishraq Zafar, Shi Lu, Honggang Li
Summary: The fertilizing spermatozoon is a highly specialized cell that plays a crucial role in fertilization and embryo development. PLC zeta and PAWP are main candidates as oocyte activated factors during fertilization, but their mechanisms and treatment methods still need further research and exploration.
CELL AND BIOSCIENCE
(2021)
Article
Genetics & Heredity
Sophie Popkiss, Fabrizzio Horta, Beverley Vollenhoven, Mark P. Green, Deirdre Zander-Fox
Summary: This study aimed to investigate the effect of calcium chloride dihydrate supplementation on different fertilization rate groups during the ICSI process. The results showed that adding calcium significantly improved fertilization rate and clinical pregnancy rate in patients with low fertilization rates (< 50%), but had minimal impact on patients with normal fertilization rates.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2022)
Article
Endocrinology & Metabolism
Jing Ling Ruan, Shan Shan Liang, Jia Ping Pan, Zhi Qin Chen, Xiao Ming Teng
Summary: This retrospective study found that artificial oocyte activation (AOA) with calcium ionophore ionomycin can significantly improve the reproductive outcomes of patients with previous fertilization failure and poor embryo development after intracytoplasmic sperm injection (ICSI). AOA can increase fertilization rate, embryo quality, implantation rate, clinical pregnancy rate, and live birth rate, while decreasing miscarriage rate. The study suggests that AOA is an effective and safe treatment option for some infertility patients.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Cell Biology
Wjdan S. Bafleh, Haia M. R. Abdulsamad, Sally M. Al-Qaraghuli, Riwa Y. El Khatib, Rawdah Taha Elbahrawi, Azhar Mohamud Abdukadir, Shaima M. Alsawae, Zakia Dimassi, Hamdan Hamdan, Junaid Kashir
Summary: The recent COVID-19 pandemic has brought about significant changes in various fields, including society, law, economics, science, and medicine. The use of mRNA-based vaccines has been approved for the first time by drug regulatory authorities to combat the outbreak. While this represents a novel application of mRNA technology in vaccination medicine, the introduction of RNA into cells to produce molecules is not a new concept. It has been commonly practiced in research to manipulate oocytes and embryos and has potential therapeutic and diagnostic applications in combating infertility. This article describes the potential clinical applications, advantages, and limitations of mRNA-based platforms, and discusses how recent advances driven by the pandemic can benefit infertility treatment. Future directions for enhancing RNA therapeutics in reproductive biology, specifically in oocyte and embryo delivery, are also presented.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Endocrinology & Metabolism
Mingru Yin, Weina Yu, Wenzhi Li, Qianqian Zhu, Hui Long, Pengcheng Kong, Qifeng Lyu
Summary: The study found that AOA treatment affects the pre- and post-implantation embryo development in mice, resulting in increased expression of the maternal allele-specific gene Igf2r but significantly lower birth weight in newborn mice. However, no other abnormalities were observed in the offspring born following AOA treatment.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2021)
Article
Obstetrics & Gynecology
Mingru Yin, Menghui Li, Wenzhi Li, Ling Wu, Zhiguang Yan, Jilang Zhao, Jie Ouyang, Qifeng Lyu, Zheng Yan, Bin Li
Summary: The study found that artificial oocyte activation (AOA) using ionomycin 1 h after ICSI did not yield benefits in early or late embryonic development for patients with a history of embryo developmental problems.
ARCHIVES OF GYNECOLOGY AND OBSTETRICS
(2022)
Article
Environmental Sciences
Xin-Le Lai, Wen-Jie Xiong, Li-Shu Li, Meng-Fan Lan, Jin-Xin Zhang, Yu-Ting Zhou, Dong Niu, Xing Duan
Summary: Zinc, an essential trace mineral, plays a critical role in various cellular processes. Zinc deficiency in patients with polycystic ovary syndrome (PCOS) has been found to affect the reproductive system, particularly oocyte quality. This study established an in vitro model to investigate the regulatory function of zinc in porcine oocyte maturation. The results showed that zinc deficiency disrupted meiotic progress and cytoskeleton structure, impaired mitochondrial function, perturbed cytosolic Ca2+ homeostasis, and induced mitophagy in porcine oocytes.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Genetics & Heredity
Omar Shebl, Philip Sebastian Trautner, Sabine Enengl, Elisabeth Reiter, Christina Allerstorfer, Tamara Rechberger, Peter Oppelt, Thomas Ebner
Summary: The application of ionophore at the oocyte stage in cases of artificial oocyte activation positively influences pronuclei formation and the synchrony of the third cell cycle, improving fertilization and blastocyst utilization rates. The treatment does not affect cleavage timing negatively or lead to irregular cleavage.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Endocrinology & Metabolism
Qinli Liu, Sen Zhao, Jian Zhou, Ping Liu, Bo Huo
Summary: This study found that continuous microvibration stimulation can significantly improve the parthenogenetic developmental potential of human immature oocytes.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Environmental Sciences
Yong-Sheng Wang, Sheng-Ji Yang, Muhammad Jamil Ahmad, Zhi-Ming Ding, Ze-Qun Duan, Yang-Wu Chen, Ming Liu, Ai-Xin Liang, Guo-Hua Hua, Li-Jun Huo
Summary: This study investigated the adverse effects of Zinc Pyrithione (ZPT) on mouse oocyte maturation and quality. The results showed that ZPT compromised oocyte maturation by disrupting meiotic cell cycle and causing cytoskeletal defects. In addition, ZPT altered epigenetic modifications and resulted in zinc accumulation, which further impaired the quality of oocytes.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Reproductive Biology
Zhengda Li, Ziyun Ruan, Xin Zhao, Xiling Qin, Jun Zhang, Yun Feng, Jiaka Lu, Deshun Shi, Fenghua Lu
Summary: This study reveals the importance of regulating Xist gene expression in improving the developmental ability of buffalo SCNT-female embryos, providing further insights into the role of Xist in embryonic development.
Article
Biochemistry & Molecular Biology
Kyungjun Uh, Junghyun Ryu, Kayla Farrell, Noah Wax, Kiho Lee
Article
Multidisciplinary Sciences
Katy S. Stoecklein, M. Sofia Ortega, Lee D. Spate, Clifton N. Murphy, Randall S. Prather
Summary: This study investigated the potential of adding a combination of three cytokines (FGF2, LIF, and IGF1) named FLI to improve the development and quality of in vitro produced bovine embryos. Results showed that the addition of FLI increased the success rate of oocyte maturation and blastocyst development, as well as improved the quality of embryos by reducing lipid content and enhancing cytoskeleton integrity. This supplementation also decreased cell apoptosis post-thaw, indicating its potential to alleviate challenges associated with cryo-survival of in vitro produced bovine embryos.
Article
Multidisciplinary Sciences
Krishanu Saha, Erik J. Sontheimer, P. J. Brooks, Melinda R. Dwinell, Charles A. Gersbach, David R. Liu, Stephen A. Murray, Shengdar Q. Tsai, Ross C. Wilson, Daniel G. Anderson, Aravind Asokan, Jillian F. Banfield, Krystof S. Bankiewicz, Gang Bao, Jeff W. M. Bulte, Nenad Bursac, Jarryd M. Campbell, Daniel F. Carlson, Elliot L. Chaikof, Zheng-Yi Chen, R. Holland Cheng, Karl J. Clark, David T. Curiel, James E. Dahlman, Benjamin E. Deverman, Mary E. Dickinson, Jennifer A. Doudna, Stephen C. Ekker, Marina E. Emborg, Guoping Feng, Benjamin S. Freedman, David M. Gamm, Guangping Gao, Ionita C. Ghiran, Peter M. Glazer, Shaoqin Gong, Jason D. Heaney, Jon D. Hennebold, John T. Hinson, Anastasia Khvorova, Samira Kiani, William R. Lagor, Kit S. Lam, Kam W. Leong, Jon E. Levine, Jennifer A. Lewis, Cathleen M. Lutz, Danith H. Ly, Samantha Maragh, Paul B. McCray, Todd C. McDevitt, Oleg Mirochnitchenko, Ryuji Morizane, Niren Murthy, Randall S. Prather, John A. Ronald, Subhojit Roy, Sushmita Roy, Venkata Sabbisetti, W. Mark Saltzman, Philip J. Santangelo, David J. Segal, Mary Shimoyama, Melissa C. Skala, Alice F. Tarantal, John C. Tilton, George A. Truskey, Moriel Vandsburger, Jonathan K. Watts, Kevin D. Wells, Scot A. Wolfe, Qiaobing Xu, Wen Xue, Guohua Yi, Jiangbing Zhou
Summary: The NIH's SCGE Consortium aims to develop safer and more effective methods to edit disease-relevant somatic cell genomes in patients, even in hard-to-reach tissues. Their approach includes rigorous validation of technology through third-party testing in animals to accelerate clinical development of new therapies.
Review
Developmental Biology
Rodney D. Geisert, Ashley E. Meyer, Caroline A. Pfeiffer, Destiny N. Johns, Kiho Lee, Kevin D. Wells, Thomas E. Spencer, Randall S. Prather
Summary: The development of viviparity in mammals requires the evolution of the placenta as an intermediate interface between the fetus and maternal uterus, as well as the modification or inhibition of the maternal immune system. CRISPR-Cas9 gene-editing technology has provided a direct approach to investigate the specific role of conceptus factors in pregnancy establishment and maintenance, altering our understanding of maternal recognition and maintenance of pregnancy in pigs.
Article
Multidisciplinary Sciences
Sweta Ravisankar, Kelsey E. Brooks, Melinda J. Murphy, Nash Redmayne, Junghyun Ryu, Jason M. Kinchen, Shawn L. Chavez, Jon D. Hennebold
Summary: The administration of gonadotropins during infertility treatment affects the development of ovarian follicles and the competency of oocytes. Metabolomics analysis identified unique metabolites in follicular fluid that were associated with different embryo classifications, with an increase in the cortisol to cortisone ratio observed in the blastocyst group. The glucocorticoid receptor NR3C1 plays a role in regulating follicular processes through paracrine signaling, but further studies are needed to understand its specific mechanisms.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Paula R. Chen, Caroline G. Lucas, Lee D. Spate, Randall S. Prather
Summary: Supplementation with glutamine in porcine embryo culture medium improves development, increases leucine consumption, and enhances mitochondrial activity. Glutaminolysis is involved in the activation of mTORC1 in porcine embryos, as evidenced by changes in phosphorylated MTOR levels and colocalization with lysosomal markers. Further studies are needed to understand the downstream effects on development.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2021)
Correction
Multidisciplinary Sciences
Sweta Ravisankar, Carol B. Hanna, Kelsey E. Brooks, Melinda J. Murphy, Nash Redmayne, Junghyun Ryu, Jason M. Kinchen, Shawn L. Chavez, Jon D. Hennebold
SCIENTIFIC REPORTS
(2021)
Article
Cardiac & Cardiovascular Systems
George C. Gabriel, William Devine, Bethany K. Redel, Kristin M. Whitworth, Melissa Samuel, Lee D. Spate, Raissa F. Cecil, Randall S. Prather, Yijen Wu, Kevin D. Wells, Cecilia W. Lo
Summary: Modeling cardiovascular diseases in pigs has revealed similar cardiac development processes to humans and mice, showing potential for preclinical studies focusing on congenital heart disease improvement. Genetically edited pigs with congenital heart defects could serve as valuable models for further research.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2021)
Article
Reproductive Biology
C. G. Lucas, B. K. Redel, P. R. Chen, L. D. Spate, R. S. Prather, K. D. Wells
Summary: Pigs have become important models for agricultural and biomedical purposes, with the use of genomic engineering tools like CRISPR/Cas9 to produce livestock models with desired modifications. Enhancing HDR events in pig embryos using the compound RS-1 has shown promising results, with no adverse effects on early embryo development. Combining CRISPR/Cas9 with HDR strategies, like utilizing RS-1, is a key tool to simplify and accelerate the production of genetically modified livestock models.
REPRODUCTIVE TOXICOLOGY
(2021)
Review
Cell Biology
Paula R. Chen, Bethany K. Redel, Karl C. Kerns, Lee D. Spate, Randall S. Prather
Summary: The review discusses the application of genetically modified pigs in animal agriculture and human medicine, focusing on the importance of in vitro production and manipulation of embryos. To improve porcine embryo culture systems, consideration of metabolic characteristics, comparisons with other mammalian species, and addressing stressors from environment and parental factors are essential. Progressing stepwise through in vitro oocyte maturation, fertilization, and embryo culture in pigs can help assess current systems and identify areas for improvement.
Article
Medicine, Research & Experimental
Junghyun Ryu, William Chan, Jochen M. Wettengel, Carol B. Hanna, Benjamin J. Burwitz, Jon D. Hennebold, Benjamin N. Bimber
Summary: In this study, the authors present a method called esTag-PCR for mapping transgene integration and copy number. They demonstrate that esTag-PCR has higher accuracy and efficiency compared to alternative methods and can be used to validate and screen edited embryos before implantation. This technique represents an important advancement in the field of genome engineering.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2022)
Review
Biochemistry & Molecular Biology
Rodney D. Geisert, Destiny N. Johns, Caroline A. Pfeiffer, Riley M. Sullivan, Caroline G. Lucas, Constantine A. Simintiras, Bethany K. Redel, Kevin D. Wells, Thomas E. Spencer, Randall S. Prather
Summary: CRISPR-Cas9 gene editing technology allows for the study of specific genes involved in reproductive regulation, particularly in the establishment and maintenance of pregnancy. Research on gene editing of porcine conceptuses provides new insights into the regulation of endometrial function, while in vitro studies using endometrial organoids offer an alternative method for studying specific endometrial genes.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2023)
Article
Multidisciplinary Sciences
Junghyun Ryu, John P. Statz, William Chan, Fernanda C. Burch, John V. Brigande, Beth Kempton, Edward V. Porsov, Lauren Renner, Trevor McGill, Benjamin J. Burwitz, Carol B. Hanna, Martha Neuringer, Jon D. Hennebold
Summary: In this study, a nonhuman primate model of Usher syndrome type 1B (USH1B) was created using CRISPR/Cas9 technology. The efficiency of different CRISPR components was compared, and the desired mutations were confirmed by preimplantation genetic testing (PGT). This study demonstrates the feasibility of using CRISPR technologies to create NHP models of human diseases.
SCIENTIFIC REPORTS
(2022)
Article
Cardiac & Cardiovascular Systems
Katherine Bishara, Jennie H. H. Kwon, Morgan A. A. Hill, Kristi L. L. Helke, Russell A. A. Norris, Kristin Whitworth, Randall S. S. Prather, Taufiek Konrad Rajab
Summary: A transgenic strain of pigs expressing green fluorescent protein (GFP) was created, and GFP expression in the semilunar valves and great arteries was characterized. Immunofluorescence was used to visualize and quantify GFP expression and its colocalization with nuclear staining. GFP expression was confirmed in both the semilunar valves and great arteries of the transgenic pigs.
JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE
(2023)
Review
Agriculture, Multidisciplinary
Kristin M. M. Whitworth, Jonathan A. A. Green, Bethany K. K. Redel, Rodney D. D. Geisert, Kiho Lee, Bhanu P. P. Telugu, Kevin D. D. Wells, Randall S. S. Prather
Summary: Genetic modification of animals through selective breeding is essential for modern agriculture, but it has limitations. In order to sustainably improve animal production and feed the growing global population, responsive and precise breeding strategies are needed. The emergence of genome editing tools provides a solution, and germline engineering in pigs is a significant development in this field.
CABI AGRICULTURE & BIOSCIENCE
(2022)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.