Article
Agriculture, Dairy & Animal Science
Adel R. Moawad, Ali Salama, Magdy R. Badr, Mohamed Fathi
Summary: Supplementation of L-Carnitine (LC) during in vitro maturation (IVM) of canine oocytes improves maturation, fertilization, and preimplantation embryo development rates following IVF and in vitro culture (IVC).
Review
Reproductive Biology
Gabriela Ramos Leal, Clara Ana Santos Monteiro, Luciano de Rezende Carvalheira, Joanna M. G. Souza-Fabjan
Summary: Simulated Physiological Oocyte Maturation (SPOM) is an in vitro method that mimics the physiological events of oocyte maturation using cAMP modulators. This study systematically analyzes the literature on the use of SPOM and compares the outcomes to the original study. The results indicate difficulties in reproducing the SPOM system worldwide and suggest that meiosis arrest alone is not sufficient for successful SPOM application. The different supplements used in the IVM medium and their interaction with modulators may impact experimental success.
Article
Biochemistry & Molecular Biology
Ralf Poehland, Jens Vanselow, Fabiana Melo Sterza
Summary: This study investigated the presence of HES1 in both compartments of bovine cumulus oocyte complexes (COCs) and its distribution after in vitro maturation (IVM). HES1 mRNA was detectable in oocytes but not in cumulus cells, while the protein was mainly visible in cumulus cells. GFP-HES1-mRNA injection showed signal in both oocytes and cumulus cells, suggesting protein transfer from oocytes to cumulus cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Obstetrics & Gynecology
Robert B. Gilchrist, Tuong M. Ho, Michel De Vos, Flor Sanchez, Sergio Romero, William L. Ledger, Ellen Anckaert, Lan N. Vuong, Johan Smitz
Summary: Recent advances in understanding oocyte biology and ovulation from animal studies have led to the development of biphasic IVM approaches, which involve pre-culturing immature oocytes before routine in vitro maturation. This novel procedure has the potential to significantly impact human ART practices. This review examines the scientific advances in ovarian biology, biphasic IVM procedures, and reports on outcomes from animal and clinical human data.
HUMAN REPRODUCTION UPDATE
(2023)
Article
Biology
Daniela Nogueira, Carole Fajau-Prevot, Muriel Clouet, Patrick Assouline, Marion Deslandres, Marie Montagut
Summary: To ensure patient care in an oncological fertility preservation programme, the best technology must be provided to suit the patients' clinical conditions. In vitro oocyte maturation (IVM) and ovarian tissue cryopreservation (OTC) are effective methods for fertility preservation in women requiring urgent oncological treatment. Further research is needed to fully assess the technical consistency, efficacy, and safety of IVM using immature oocytes collected through different methods.
Article
Multidisciplinary Sciences
Kaori Nozawa, Zian Liao, Yuhkoh Satouh, Ting Geng, Masahito Ikawa, Diana Monsivais, Martin M. Matzuk
Summary: Wee1-like protein kinase 2 (WEE2) is an oocyte-specific protein tyrosine kinase involved in the regulation of oocyte meiotic arrest. Knockout mouse models were developed to test the impact of Wee2 loss-of-function on female fertility. While a frameshift mutation had no effect, a larger deletion resulted in reduced litter size in homozygous Wee2 female knockout mice. Overall, loss of Wee2 had only minor effects on fertility in mice, suggesting alternative approaches should be considered for contraceptive development.
Article
Reproductive Biology
Dulama Richani, Robert B. Gilchrist
Summary: Oocytes are maintained in a state of meiotic arrest until ovulation is triggered. Manipulation of cyclic nucleotides such as cGMP and cAMP can influence meiotic arrest and improve oocyte developmental competence. Pre-IVM modulation of cGMP and/or cAMP has shown consistent improvement in oocyte developmental competence.
BIOLOGY OF REPRODUCTION
(2022)
Article
Reproductive Biology
Nazli Akin, Lucia von Mengden, Anamaria-Cristina Herta, Katy Billooye, Julia van Leersum, Berta Cava-Cami, Laura Saucedo-Cuevas, Fabio Klamt, Johan Smitz, Ellen Anckaert
Summary: The novel two-step IVM method CAPA-IVM has improved embryology outcomes by synchronizing oocyte nuclear and cytoplasmic maturation, although there is still a noticeable efficiency gap compared with traditional IVF. Glucose metabolism studies have revealed metabolic shortcomings within CAPA-IVM COCs, with specific alterations in mouse COC glucose metabolism characterized for the first time. Future optimization strategies for CAPA-IVM could focus on adjusting culture media energy substrate concentrations and/or implementing co-culture strategies.
BIOLOGY OF REPRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Ahmed Atef Mesalam, Marwa El-Sheikh, Myeong-Don Joo, Atif Ali Khan Khalil, Ayman Mesalam, Mi-Jeong Ahn, Il-Keun Kong
Summary: Exposing bovine oocytes to juglone at concentrations of 12.5-50.0 μM significantly impairs their development by inducing ROS accumulation, apoptosis, and mitochondrial dysfunction. This study provides insights into the adverse effects of juglone treatment on oocyte maturation and embryo development, highlighting the importance of understanding the impact of traditional medicinal components on reproductive processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Huaxing Zhao, Yazheng Dong, Yuxing Zhang, Xiao Wu, Xianjun Zhang, Yalin Liang, Yanan Li, Fang Zeng, Junsong Shi, Rong Zhou, Linjun Hong, Gengyuan Cai, Zhenfang Wu, Zicong Li
Summary: The addition of 20 ng/mL SDF1 during pig oocyte IVM improves the maturation rate of oocytes and the cleavage and blastocyst rates of resulting embryos. SDF1 supplementation decreases oxidative stress, enhances mitochondrial function, and influences gene expression related to apoptosis in pig oocytes.
Review
Veterinary Sciences
Shimaa I. Rakha, Mohammed A. Elmetwally, Hossam El-Sheikh Ali, Ahmed Balboula, Abdelmonem Montaser Mahmoud, Samy M. Zaabel
Summary: In vitro embryo production (IVEP) is a widely used technique in reproductive biology, with in vitro maturation (IVM) being the critical initial step. The quality of in vitro matured oocytes is lower than those matured in vivo, mainly due to the accumulation of reactive oxygen species (ROS) during IVM. Supplementation of antioxidants in the IVM medium has shown promise in preventing ROS accumulation and improving oocyte quality.
VETERINARY SCIENCES
(2022)
Article
Environmental Sciences
Zhao-Bo Luo, Mei-Fu Xuan, Sheng-Zhong Han, Zhou-Yan Li, Nasar Khan, Biao-Hu Quan, Xi-Jun Yin, Jin-Dan Kang
Summary: The study demonstrated that ginsenoside Rb1 can protect porcine oocytes from the detrimental effects of methylglyoxal, enhancing maturation rate and improving in vitro developmental capacity, offering novel perspectives for the protection of the reproductive system through the use of ginseng as a functional food.
ENVIRONMENTAL TOXICOLOGY
(2021)
Article
Obstetrics & Gynecology
Jade Raad, Charlotte Sonigo, Alexandra Benoit, Isabelle Cedrin-Durnerin, Christophe Sifer, Nathalie Sermondade, Michael Grynberg
Summary: This study investigates the influence of breast cancer (BC) characteristics on in vitro maturation (IVM) outcomes of oocytes in patients undergoing fertility preservation (FP). The study found that Scarff-Bloom-Richardson (SBR) III grade, triple-negative BC, and HER2 overexpression are independent predictors of fewer oocytes or poor IVM outcomes in young women seeking FP.
HUMAN REPRODUCTION
(2022)
Article
Cell Biology
Jing Wang, Zhiyong Zhuo, Xiao Ma, Yunjie Liu, Jing Xu, Changjiu He, Yao Fu, Feng Wang, Pengyun Ji, Lu Zhang, Guoshi Liu
Summary: The study found that melatonin protects against nuclear inhibition caused by hypoxanthine and restores oocyte meiosis via MT1 by reducing the intracellular concentration of cAMP.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Yuting Xiang, Chuanchuan Zhou, Yanyan Zeng, Qi Guo, Jiana Huang, Taibao Wu, Jiawen Liu, Qiqi Liang, Haitao Zeng, Xiaoyan Liang
Summary: N4-acetylcytidine (ac4C), an epigenetic modification within mRNA, is crucial in regulating mRNA stability and translation efficiency. NAT10 is the enzyme responsible for ac4C production in mammals, and its knockdown delays meiotic maturation in vitro. Identification of TBL3 as a potential ac4C-binding protein suggests its role in downstream cellular activities.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.