Article
Reproductive Biology
Hwanhee Jang, Soohyung Lee, Inkyu Yoo, Yohan Choi, Jisoo Han, Yugyeong Cheon, Hakhyun Ka
Summary: S100A8, S100A9, and S100A12, calcium-binding proteins, are dynamically expressed in response to conceptus-derived signals at the maternal-conceptus interface, potentially playing a critical role in regulating endometrial epithelial cell function and conceptus implantation to support the establishment and maintenance of pregnancy in pigs.
BIOLOGY OF REPRODUCTION
(2022)
Article
Agriculture, Dairy & Animal Science
Inkyu Yoo, Soohyung Lee, Yugyeong Cheon, Hakhyun Ka
Summary: This study investigated the expression, localization, and regulation of MMP2, MMP8, MMP9, MMP12, and MMP13 in the pig endometrium during the estrous cycle and pregnancy. The results showed that expression levels of these MMPs changed during different reproductive stages, suggesting their important role in endometrial remodeling and processing bioactive molecules in pigs.
Article
Reproductive Biology
Jisoo Han, Inkyu Yoo, Soohyung Lee, Yugyeong Cheon, Cheol-Heui Yun, Hakhyun Ka
Summary: The expression of IL10 and IL12 in the endometrium of pigs shows a specific pattern during pregnancy. IL10 may provide anti-inflammatory conditions for maternal immune tolerance.
BIOLOGY OF REPRODUCTION
(2022)
Article
Reproductive Biology
Piotr Kaczynski, Vera van der Weijden, Ewelina Goryszewska-Szczurek, Monika Baryla, Susanne E. Ulbrich, Agnieszka Waclawik
Summary: This study found that CpG methylation induced by embryonic signals may contribute to regulating endometrial gene expression during early pregnancy in pigs.
BIOLOGY OF REPRODUCTION
(2022)
Article
Veterinary Sciences
Wonchul Jung, Inkyu Yoo, Jisoo Han, Minjeong Kim, Soohyung Lee, Yugeong Cheon, Minsun Hong, Bo-Young Jeon, Hakhyun Ka
Summary: Research has shown that caspases are dynamically expressed in the endometrium throughout the estrous cycle and at the maternal-conceptus interface during pregnancy in pigs. The expression of CASP3, CASP6, and CASP7 changes in a stage-specific manner in the endometrium during different reproductive stages. Additionally, the expression of caspases in endometrial cells is influenced by steroid hormones and cytokines, suggesting that caspase action may play an important role in regulating endometrial and placental function in pigs during the implantation period.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Reproductive Biology
Eleanore O'Neil, Thomas E. Spencer
Summary: This study found that certain lipid components are reduced in the endometrium of pregnant ewes, while certain lipid compounds are elevated in the uterine lumen. Additionally, elevated levels of amino acids, carbohydrates, and energy substrates were observed in the uterine lumen of pregnant ewes.
BIOLOGY OF REPRODUCTION
(2021)
Article
Reproductive Biology
Destiny N. Johns, Caroline G. Lucas, Caroline A. Pfeiffer, Paula R. Chen, Ashley E. Meyer, Shelbi D. Perry, Lee D. Spate, Raissa F. Cecil, Melissa A. Fudge, Melissa S. Samuel, Christine M. Spinka, Hongyu Liu, Matthew C. Lucy, Kevin D. Wells, Randall S. Prather, Thomas E. Spencer, Rodney D. Geisert
Summary: The study revealed that production of IFNG by the conceptus is crucial for attachment and survival in pigs, as lack of IFNG resulted in degeneration and endometrial inflammation.
BIOLOGY OF REPRODUCTION
(2021)
Article
Agriculture, Dairy & Animal Science
Soohyung Lee, Inkyun Yoo, Yugyeong Cheon, Hakhyun Ka
Summary: This study investigated the expression of SAA3 in the pig endometrium during the estrous cycle and pregnancy. It was found that SAA3 had the highest expression on day 12 of pregnancy and was primarily localized to the luminal epithelial cells. In vitro cultures showed that SAA3 expression was induced by IL1B and IFNG. These findings suggest that SAA3 expression in the endometrium is regulated by conceptus-derived IL1B and IFNG, and it could serve as a specific marker for conceptus implantation in pigs.
Article
Agriculture, Dairy & Animal Science
Agnieszka Blitek, Mateusz Luba, Magdalena Szymanska
Summary: The study reveals changes in PGI2 synthesis in the myometrium of pigs during the estrous cycle and early pregnancy, with PGI2 playing a role in regulating smooth muscle activity and inflammatory response factors. The results suggest a significant role of PGI2 in porcine myometrium function modulation.
Article
Agriculture, Dairy & Animal Science
Benjamin R. Crites, Sarah N. Carr, Leslie H. Anderson, James C. Matthews, Phillip J. Bridges
Summary: This study investigated the effects of different forms of selenium supplementation on endometrial function and conceptus development in heifers. The results showed that a mixed form of selenium supplement could improve fertility in cattle. This is important for cattle in regions with selenium-deficient soils for optimal immune function, growth, and fertility.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Veterinary Sciences
Charlotte Gibson, M. de Ruijter-Villani, Tom A. E. Stout
Summary: In the equine pre-implantation development, the insulin-like growth factors (IGFs) system plays an important role in preparing the endometrium for implantation and regulating conceptus growth and development. The presence and down-regulation of IGFs, their receptors, and IGF binding proteins (IGFBPs) in both the endometrium and conceptus suggest their involvement in conceptus-maternal communication during the preparation for implantation.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Piotr Kaczynski, Ewelina Goryszewska-Szczurek, Monika Baryla, Agnieszka Waclawik
Summary: The establishment of pregnancy in mammals, including pigs, requires communication between developing embryos and the maternal organism. Pigs signalize their presence by secreting factors such as estradiol-17 beta (E2), initiating the maternal recognition of pregnancy. Prostaglandin (PGF2 alpha) and luteoprotective factors contribute to the maintenance of progesterone secretion, preparing the uterus for implantation. Embryo implantation involves a proinflammatory environment coordinated by the secretion of cytokines, growth factors, and lipid mediators. Recent studies have identified novel factors and revealed complex relationships with established factors. Early pregnancy is a sequence of processes orchestrated by pleiotropic factors, preserving essential functions critical for implantation and placenta formation.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2023)
Article
Reproductive Biology
Heather L. Chaney, Lindsay F. Grose, Jeanna M. LaBarbara, Adam W. Sirk, Alyssa M. Blancke, Jose M. Sanchez, Claudia Passaro, Patrick Lonergan, Daniel J. Mathew
Summary: Bovine and ovine conceptuses express galectin-1, which induces endometrial gene expression related to maternal immune tolerance in cattle. Expression of galectins in the endometrium varies during pregnancy in cows and sheep, suggesting important roles in maternal-conceptus immune tolerance in ruminants.
BIOLOGY OF REPRODUCTION
(2022)
Article
Reproductive Biology
James W. Frank, Chelsie B. Steinhauser, Xiaoqiu Wang, Robert C. Burghardt, Fuller W. Bazer, Greg A. Johnson
Summary: During the peri-implantation period of pregnancy in sheep, blocking ITGB3 expression in conceptuses did not prevent elongation and implantation, but decreased embryo growth and expression of SPP1 and NOS3. ITGB3 may play a role in regulating vasculature development within the allantois, impacting nutrient transport and embryo growth.
BIOLOGY OF REPRODUCTION
(2021)
Article
Reproductive Biology
Marlena Gudelska, Kamil Dobrzyn, Marta Kiezun, Katarzyna Kisielewska, Edyta Rytelewska, Tadeusz Kaminski, Nina Smolinska
Summary: This study aimed to investigate the influence of steroid hormones on the expression of the chemerin system in the uterus of domestic pigs. The results showed that both estradiol and progesterone can modulate the expression of chemerin receptor genes and proteins, as well as the abundance of RARRES2 mRNA transcripts. Additionally, estradiol increased chemerin protein secretion during the maternal recognition of pregnancy and decreased its release during embryo implantation.
Article
Agriculture, Dairy & Animal Science
A. Zlotkowska, A. Andronowska
DOMESTIC ANIMAL ENDOCRINOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Mohammad Mehedi Hasan, Janeli Viil, Freddy Lattekivi, James Ord, Qurat Ul Ain Reshi, Kersti Jaager, Agne Velthut-Meikas, Aneta Andronowska, Ulle Jaakma, Andres Salumets, Alireza Fazeli
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Nina Schmid, Kim-Gwendolyn Dietrich, Ignasi Forne, Alexander Burges, Magdalena Szymanska, Rina Meidan, Doris Mayr, Artur Mayerhofer
Summary: SIRT1 and SIRT3 are involved in the growth regulation of granulosa cell tumor cells, and SIRTs may represent novel drug targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Izabela Malysz-Cymborska, Dominika Golubczyk, Lukasz Kalkowski, Joanna Kwiatkowska, Michal Zawadzki, Joanna Glodek, Piotr Holak, Joanna Sanford, Kamila Milewska, Zbigniew Adamiak, Piotr Walczak, Miroslaw Janowski
Summary: Stem cell transplantation through the intra-arterial route for treating neurodegenerative diseases like ALS was investigated for feasibility and safety, showing promising results in canine and porcine models.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Magdalena Szymanska, Ketan Shrestha, Eliezer Girsh, Avi Harlev, Iris Eisenberg, Tal Imbar, Rina Meidan
Summary: Granulosa-lutein cells (GLCs) from PCOS women exhibit reduced levels of HIF-1 alpha and EDN2, with microRNA-210 playing a key role in regulating this process. The disrupted hypoxic response in the GLCs of periovulatory follicles in PCOS may contribute to ovulation failure and reduced fertility in this syndrome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Mohammad Mehedi Hasan, Qurat Ul Ain Re, Freddy Lattekivi, Janeli Viil, Kasun Godakumara, Keerthie Dissanayake, Aneta Andronowska, Ulle Jaakma, Alireza Fazeli
Summary: Before the union of an egg and spermatozoon, multiple vital processes occur in the female reproductive system. The maturation of spermatozoa in the female reproductive tract is crucial for fertilization. Follicular fluid (FF) and its extracellular vesicles (EVs) play a significant role in supporting sperm functions, with specific effects based on species and source of FF EVs. This study demonstrates that bovine FF EVs can support sperm function and contribute to a favorable periconceptional microenvironment.
Article
Biochemistry & Molecular Biology
Freddy Lattekivi, Irina Guljavina, Getnet Midekessa, Janeli Viil, Paul R. Heath, Rikke Baek, Malene Moller Jorgensen, Aneta Andronowska, Kulli Kingo, Alireza Fazeli
Summary: Psoriasis vulgaris (PsV) and psoriatic arthritis (PsA) are inflammatory diseases with unresolved pathophysiological aspects. In this study, the miRNA contents and surface proteome of blood serum extracellular vesicles (EVs) were compared between PsV and PsA patients and healthy controls. It was found that there were differentially enriched EV-bound miRNAs in both PsV and PsA groups, suggesting that EVs may play a role in the pathophysiological processes of PsV and PsA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Endocrinology & Metabolism
Jackson Sapuleni, Magdalena Szymanska, Rina Meidan
Summary: By activating SIRT1, key ovulatory and angiogenic genes are upregulated in human granulosa-lutein cells, but this also leads to the advancement of cell death pathways. These effects appear to be regulated by cAMP.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Agnieszka Blitek, Mateusz Luba, Magdalena Szymanska
Summary: The study reveals changes in PGI2 synthesis in the myometrium of pigs during the estrous cycle and early pregnancy, with PGI2 playing a role in regulating smooth muscle activity and inflammatory response factors. The results suggest a significant role of PGI2 in porcine myometrium function modulation.
Article
Veterinary Sciences
Johanna Piibor, Keerthie Dissanayake, Getnet Midekessa, Aneta Andronowska, Ants Kavak, Andres Waldmann, Alireza Fazeli
Summary: A comparison was made between the follicular and luteal phases of the oestrous cycle in bovine uterine fluid (UF), revealing differences in the concentration and protein profile of extracellular vesicles (EV). These findings suggest that EV modulate the uterine microenvironment throughout the oestrous cycle.
VETERINARY RESEARCH COMMUNICATIONS
(2023)
Article
Cell Biology
Qurat Ul Ain Reshi, Kasun Godakumara, James Ord, Keerthie Dissanayake, Mohammad Mehedi Hasan, Aneta Andronowska, Paul Heath, Alireza Fazeli
Summary: Spermatozoa can alter the gene expression in female reproductive tract epithelial cells both remotely and through contact, and can also change the cargo of extracellular vesicles derived from these cells, which may play an important role in pre and post-fertilization events.
JOURNAL OF CELL COMMUNICATION AND SIGNALING
(2023)
Article
Multidisciplinary Sciences
Magdalena Szymanska, Agnieszka Blitek
Summary: In this study, the researchers investigated the regulation of prostacyclin (PGI2) synthesis and PGI2 receptor (PTGIR) expression by cytokines, and evaluated the effect of PGI2 on pro-angiogenic gene expression, cell proliferation and migration, cell cycle distribution, and capillary-like structure formation in porcine endometrial cells. They found that IL1ss, IFNgimel, and/or TNFα increased PGI2 secretion and PTGIR expression. PGI2 enhanced pro-angiogenic gene expression, cell proliferation, and cell cycle progression.
SCIENTIFIC REPORTS
(2023)
Article
Agriculture, Dairy & Animal Science
Magdalena Szymanska, Agnieszka Blitek
Summary: The study examined the impact of impaired progesterone synthesis on uterine receptor expression in pigs. It was found that impaired progesterone synthesis led to decreased luteal progesterone levels during pregnancy and affected receptor expression in both the endometrium and myometrium, with the endometrium being more sensitive to impaired progesterone synthesis.
ANNALS OF ANIMAL SCIENCE
(2023)
Article
Reproductive Biology
Magdalena Szymanska, Sarah Manthe, Ketan Shrestha, Eliezer Girsh, Avi Harlev, Tatiana Kisliouk, Rina Meidan
Summary: The study indicated that SIRT1 can suppress the expression of EDN2 in granulosa-lutein cells by regulating HIF1A and histone modification, thereby affecting cell survival.
BIOLOGY OF REPRODUCTION
(2021)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.