Journal
OPTICS EXPRESS
Volume 20, Issue 13, Pages 13958-13965Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.20.013958
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- Grants-in-Aid for Scientific Research [21248040, 23656048] Funding Source: KAKEN
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We propose the collinear balanced detection (CBD) technique for noise suppression in fiber laser (FL)-based stimulated Raman scattering (SRS) microscopy. This technique reduces the effect of laser intensity noise at a specific frequency by means of pulse splitting and recombination with a time delay difference. We experimentally confirm that CBD can suppress the intensity noise of second harmonic (SH) of Er-FL pulses by 13dB. The measured noise level including the thermal noise is higher by only similar to 1.4 dB than the shot noise limit. To demonstrate SRS imaging, we use 4-ps SH pulses and 3-ps Yb-FL pulses, which are synchronized subharmonically with a jitter of 227 fs. The effectiveness of the CBD technique is confirmed through SRS imaging of a cultured HeLa cell. (C) 2012 Optical Society of America
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